Yoshihiro Ishikawa, PhD
Assistant Professor
Ophthalmology
School of Medicine

He has functionally characterized over ten collagen-related biosynthetic molecules, including rER chaperones and enzymes required for posttranslational modifications. Furthermore, his in vitro studies identified unique patterns on collagen posttranslational modifications in different collagen types and specific molecular interactions between rER proteins, collagens and other extracellular matrix proteins. While many studies on type IV collagen and its mutations using cell biology and model organisms have been reported, it’s still challenging to investigate the details of type IV collagen molecules with biochemical and biophysical approaches. To fill this important gap in our knowledge, he is uniquely qualified to conduct type IV collagen biochemistry and biophysics at UCSF.

• Aberrant binding of mutant HSP47 affects posttranslational modification of type I collagen and leads to osteogenesis imperfecta.
  PLoS genetics 2021 Syx D, Ishikawa Y, Gebauer J, Boudko SP, Guillemyn B, Van Damme T, D'hondt S, Symoens S, Nampoothiri S, Gould DB, Baumann U, Bächinger HP, Malfait F
• The novel missense mutation Met48Lys in FKBP22 changes its structure and functions.
  Scientific reports 2020 Ishikawa Y, Mizuno N, Holden P, Lim PJ, Gould DB, Rohrbach M, Giunta C, Bächinger HP
• The HDAC3-SMARCA4-miR-27a axis promotes expression of the PAX3:FOXO1 fusion oncogene in rhabdomyosarcoma.
  Science signaling 2018 Bharathy N, Berlow NE, Wang E, Abraham J, Settelmeyer TP, Hooper JE, Svalina MN, Ishikawa Y, Zientek K, Bajwa Z, Goros MW, Hernandez BS, Wolff JE, Rudek MA, Xu L, Anders NM, Pal R, Harrold AP, Davies …
• The endoplasmic reticulum-resident collagen chaperone Hsp47 interacts with and promotes the secretion of decorin, fibromodulin, and lumican.
  The Journal of biological chemistry 2018 Ishikawa Y, Rubin K, Bächinger HP, Kalamajski S
• Type III collagen affects dermal and vascular collagen fibrillogenesis and tissue integrity in a mutant Col3a1 transgenic mouse model.
  Matrix biology : journal of the International Society for Matrix Biology 2018 D'hondt S, Guillemyn B, Syx D, Symoens S, De Rycke R, Vanhoutte L, Toussaint W, Lambrecht BN, De Paepe A, Keene DR, Ishikawa Y, Bächinger HP, Janssens S, Bertrand MJM, Malfait F
• Heat shock protein 47 and 65-kDa FK506-binding protein weakly but synergistically interact during collagen folding in the endoplasmic reticulum.
  The Journal of biological chemistry 2017 Ishikawa Y, Holden P, Bächinger HP
• A Novel Antifibrotic Mechanism of Nintedanib and Pirfenidone. Inhibition of Collagen Fibril Assembly.
  American journal of respiratory cell and molecular biology 2017 Knüppel L, Ishikawa Y, Aichler M, Heinzelmann K, Hatz R, Behr J, Walch A, Bächinger HP, Eickelberg O, Staab-Weijnitz CA
• Ziploc-ing the structure 2.0: Endoplasmic reticulum-resident peptidyl prolyl isomerases show different activities toward hydroxyproline.
  The Journal of biological chemistry 2017 Ishikawa Y, Mizuno K, Bächinger HP
• Intracellular mechanisms of molecular recognition and sorting for transport of large extracellular matrix molecules.
  Proceedings of the National Academy of Sciences of the United States of America 2016 Ishikawa Y, Ito S, Nagata K, Sakai LY, Bächinger HP
• An essential receptor for adeno-associated virus infection.
  Nature 2016 Pillay S, Meyer NL, Puschnik AS, Davulcu O, Diep J, Ishikawa Y, Jae LT, Wosen JE, Nagamine CM, Chapman MS, Carette JE
• Ziploc-ing the structure: Triple helix formation is coordinated by rough endoplasmic reticulum resident PPIases.
  Biochimica et biophysica acta 2015 Ishikawa Y, Boudko S, Bächinger HP
• A substrate preference for the rough endoplasmic reticulum resident protein FKBP22 during collagen biosynthesis.
  The Journal of biological chemistry 2014 Ishikawa Y, Bächinger HP
• Excessive transforming growth factor-β signaling is a common mechanism in osteogenesis imperfecta.
  Nature medicine 2014 Grafe I, Yang T, Alexander S, Homan EP, Lietman C, Jiang MM, Bertin T, Munivez E, Chen Y, Dawson B, Ishikawa Y, Weis MA, Sampath TK, Ambrose C, Eyre D, Bächinger HP, Lee B
• Structure of human peptidyl-prolyl cis-trans isomerase FKBP22 containing two EF-hand motifs.
  Protein science : a publication of the Protein Society 2013 Boudko SP, Ishikawa Y, Nix J, Chapman MS, Bächinger HP
• The concave face of decorin mediates reversible dimerization and collagen binding.
  The Journal of biological chemistry 2013 Islam M, Gor J, Perkins SJ, Ishikawa Y, Bächinger HP, Hohenester E
• An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1·cartilage-associated protein·cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro.
  The Journal of biological chemistry 2013 Ishikawa Y, Bächinger HP
• Posttranslational modifications in type I collagen from different tissues extracted from wild type and prolyl 3-hydroxylase 1 null mice.
  The Journal of biological chemistry 2013 Pokidysheva E, Zientek KD, Ishikawa Y, Mizuno K, Vranka JA, Montgomery NT, Keene DR, Kawaguchi T, Okuyama K, Bächinger HP
• A molecular ensemble in the rER for procollagen maturation.
  Biochimica et biophysica acta 2013 Ishikawa Y, Bächinger HP
• Crystal structures of wild-type and mutated cyclophilin B that causes hyperelastosis cutis in the American quarter horse.
  BMC research notes 2012 Boudko SP, Ishikawa Y, Lerch TF, Nix J, Chapman MS, Bächinger HP
• Mutation in cyclophilin B that causes hyperelastosis cutis in American Quarter Horse does not affect peptidylprolyl cis-trans isomerase activity but shows altered cyclophilin B-protein interactions and affects collagen folding.
  The Journal of biological chemistry 2012 Ishikawa Y, Vranka JA, Boudko SP, Pokidysheva E, Mizuno K, Zientek K, Keene DR, Rashmir-Raven AM, Nagata K, Winand NJ, Bächinger HP
• Prolyl 3-hydroxylase 1 null mice display abnormalities in fibrillar collagen-rich tissues such as tendons, skin, and bones.
  The Journal of biological chemistry 2010 Vranka JA, Pokidysheva E, Hayashi L, Zientek K, Mizuno K, Ishikawa Y, Maddox K, Tufa S, Keene DR, Klein R, Bächinger HP
• Biochemical characterization of the prolyl 3-hydroxylase 1.cartilage-associated protein.cyclophilin B complex.
  The Journal of biological chemistry 2009 Ishikawa Y, Wirz J, Vranka JA, Nagata K, Bächinger HP
• The rough endoplasmic reticulum-resident FK506-binding protein FKBP65 is a molecular chaperone that interacts with collagens.
  The Journal of biological chemistry 2008 Ishikawa Y, Vranka J, Wirz J, Nagata K, Bächinger HP