Un Seng Chio, PhD
Postdoctoral Scholar
Biochemistry and Biophysics
School of Medicine

Education & Training

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• PhD Chemistry (Biochemistry) Caltech 01/2019
• B.A. Chemistry and Biology Cornell University 05/2012

Grants and Projects

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• Molecular mechanisms of the ACF chromatin remodeling complex, NIH, 2020-2023

Publications (20)

Top publication keywords:
Guanine Nucleotide Exchange FactorsAdaptor Proteins, Vesicular TransportProtein TransportSaccharomyces cerevisiae ProteinsMolecular ChaperonesProtein BindingEndoplasmic ReticulumCryoelectron MicroscopyMembrane ProteinsCell MembraneAdenosine TriphosphatasesNucleosomesSaccharomyces cerevisiaeSirtuinsGraphite

• ATP-dependent remodeling of chromatin condensates uncovers distinct mesoscale effects of two remodelers.
  bioRxiv : the preprint server for biology 2024 Moore C, Wong E, Kaur U, Chio US, Zhou Z, Ostrowski M, Wu K, Irkliyenko I, Wang S, Ramani V, Narlikar GJ
• HMGB1 restores a dynamic chromatin environment in the presence of linker histone by deforming nucleosomal DNA.
  bioRxiv : the preprint server for biology 2024 Saunders HS, Chio US, Moore CM, Ramani V, Cheng Y, Narlikar GJ
• Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking.
  Nature communications 2024 Chio US, Palovcak E, Smith AAA, Autzen H, Muñoz EN, Yu Z, Wang F, Agard DA, Armache JP, Narlikar GJ, Cheng Y
• Nucleosome density shapes kilobase-scale regulation by a mammalian chromatin remodeler.
  Nature structural & molecular biology 2023 Abdulhay NJ, Hsieh LJ, McNally CP, Ostrowski MS, Moore CM, Ketavarapu M, Kasinathan S, Nanda AS, Wu K, Chio US, Zhou Z, Goodarzi H, Narlikar GJ, Ramani V
• Reorientation of INO80 on hexasomes reveals basis for mechanistic versatility.
  Science (New York, N.Y.) 2023 Wu H, Muñoz EN, Hsieh LJ, Chio US, Gourdet MA, Narlikar GJ, Cheng Y

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• Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking.
  bioRxiv : the preprint server for biology 2023 Chio US, Palovcak E, Autzen AAA, Autzen HE, Muñoz EN, Yu Z, Wang F, Agard DA, Armache JP, Narlikar GJ, Cheng Y
• Dodecamer assembly of a metazoan AAA+ chaperone couples substrate extraction to refolding.
  Science advances 2023 Gupta A, Lentzsch AM, Siegel A, Yu Z, Chio US, Cheng Y, Shan SO
• Cryo-EM structure of the human Sirtuin 6-nucleosome complex.
  Science advances 2023 Chio US, Rechiche O, Bryll AR, Zhu J, Leith EM, Feldman JL, Peterson CL, Tan S, Armache JP
• Computational pipeline provides mechanistic understanding of Omicron variant of concern neutralizing engineered ACE2 receptor traps.
  Structure (London, England : 1993) 2023 Remesh SG, Merz GE, Brilot AF, Chio US, Rizo AN, Pospiech TH, Lui I, Laurie MT, Glasgow J, Le CQ, Zhang Y, Diwanji D, Hernandez E, Lopez J, Mehmood H, Pawar KI, Pourmal S, Smith AM, Zhou F, QCRG …
• Subunit cooperation in the Get1/2 receptor promotes tail-anchored membrane protein insertion.
  The Journal of cell biology 2021 Chio US, Liu Y, Chung S, Shim WJ, Chandrasekar S, Weiss S, Shan SO
• CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes.
  Research square 2021 Gupta M, Azumaya CM, Moritz M, Pourmal S, Diallo A, Merz GE, Jang G, Bouhaddou M, Fossati A, Brilot AF, Diwanji D, Hernandez E, Herrera N, Kratochvil HT, Lam VL, Li F, Li Y, Nguyen HC, Nowotny C, …
• CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes.
  bioRxiv : the preprint server for biology 2021 Gupta M, Azumaya CM, Moritz M, Pourmal S, Diallo A, Merz GE, Jang G, Bouhaddou M, Fossati A, Brilot AF, Diwanji D, Hernandez E, Herrera N, Kratochvil HT, Lam VL, Li F, Li Y, Nguyen HC, Nowotny C, …
• An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike.
  Science (New York, N.Y.) 2020 Schoof M, Faust B, Saunders RA, Sangwan S, Rezelj V, Hoppe N, Boone M, Billesbølle CB, Puchades C, Azumaya CM, Kratochvil HT, Zimanyi M, Deshpande I, Liang J, Dickinson S, Nguyen HC, Chio CM, Merz GE,…
• Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms.
  Science (New York, N.Y.) 2020 Gordon DE, Hiatt J, Bouhaddou M, Rezelj VV, Ulferts S, Braberg H, Jureka AS, Obernier K, Guo JZ, Batra J, Kaake RM, Weckstein AR, Owens TW, Gupta M, Pourmal S, Titus EW, Cakir M, Soucheray M, McGregor…
• A Chaperone Lid Ensures Efficient and Privileged Client Transfer during Tail-Anchored Protein Targeting.
  Cell reports 2019 Chio US, Chung S, Weiss S, Shan SO
• In vitro Assays for Targeting and Insertion of Tail-Anchored Proteins Into the ER Membrane.
  Current protocols in cell biology 2018 Cho H, Chio US, Shan SO
• Mechanisms of Tail-Anchored Membrane Protein Targeting and Insertion.
  Annual review of cell and developmental biology 2017 Chio US, Cho H, Shan SO
• A protean clamp guides membrane targeting of tail-anchored proteins.
  Proceedings of the National Academy of Sciences of the United States of America 2017 Chio US, Chung S, Weiss S, Shan SO
• Multiple selection filters ensure accurate tail-anchored membrane protein targeting.
  eLife 2016 Rao M, Okreglak V, Chio US, Cho H, Walter P, Shan SO
• Differential gradients of interaction affinities drive efficient targeting and recycling in the GET pathway.
  Proceedings of the National Academy of Sciences of the United States of America 2014 Rome ME, Chio US, Rao M, Gristick H, Shan SO