Shaun Coughlin, MD, PhD
Professor Emeritus
Cardiovascular Research Inst
School of Medicine

Education & Training

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• Post-Doc Fellow/Scholar School of Medicine University of California, San Francisco 1986
• Residency School of Medicine University of California, San Francisco

Websites

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• Profile at UCSF Cancer Center (cancer.ucsf.edu)

Grants and Projects

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• Molecular and Cellular Basis of Cardiovascular Disease, NIH, 1992-2023
• Structural Basis of Protease-Activated Receptor Function, NIH, 2014-2017
• Structure-Function and Roles of Protease-Activated Receptors, NIH, 2017
• Molecular Genetics of Cardiac Cell Differentiation, NIH, 1996-2016
• PARs and S1P receptors in endothelial biology, NIH, 2000-2015
• Thrombin signaling in Hemostatis and thrombosis, NIH, 2000-2012
• Molecular Mechanisms of Protease Signaling, NIH, 1991-2010
• Protease-activated receptors in inflammation, NIH, 1998-2008
• Murine Models to Investigate the Hematologic System, NIH, 1995-2001
• Gordon Conference--Atherosclerosis 1993, NIH, 1993-1994
• Cell and Molecules Mediating Graft Atherosclerosis, NIH, 1989-1994

Publications (165)

Top publication keywords:
Receptors, Proteinase-ActivatedZebrafishBlood PlateletsThrombinReceptors, LysosphingolipidReceptor, PAR-1HemostasisPlatelet ActivationSignal TransductionThrombosisReceptors, ThrombinFactor XIaEndothelium, VascularReceptors, Cell SurfaceReceptor, PAR-2

• Lack of authentic atrial fibrillation in commonly used murine atrial fibrillation models.
  PloS one 2022 Fu F, Pietropaolo M, Cui L, Pandit S, Li W, Tarnavski O, Shetty SS, Liu J, Lussier JM, Murakami Y, Grewal PK, Deyneko G, Turner GM, Taggart AKP, Waters MG, Coughlin S, Adachi Y
• Microglial Gi-dependent dynamics regulate brain network hyperexcitability.
  Nature neuroscience 2020 Merlini M, Rafalski VA, Ma K, Kim KY, Bushong EA, Rios Coronado PE, Yan Z, Mendiola AS, Sozmen EG, Ryu JK, Haberl MG, Madany M, Sampson DN, Petersen MA, Bardehle S, Tognatta R, Dean T, Acevedo RM, …
• Protease-Activated Receptor-2 Regulates Neuro-Epidermal Communication in Atopic Dermatitis.
  Frontiers in immunology 2020 Buhl T, Ikoma A, Kempkes C, Cevikbas F, Sulk M, Buddenkotte J, Akiyama T, Crumrine D, Camerer E, Carstens E, Schön MP, Elias P, Coughlin SR, Steinhoff M
• Designing a Green Fluorogenic Protease Reporter by Flipping a Beta Strand of GFP for Imaging Apoptosis in Animals.
  Journal of the American Chemical Society 2019 Zhang Q, Schepis A, Huang H, Yang J, Ma W, Torra J, Zhang SQ, Yang L, Wu H, Nonell S, Dong Z, Kornberg TB, Coughlin SR, Shu X
• Entry from the Lipid Bilayer: A Possible Pathway for Inhibition of a Peptide G Protein-Coupled Receptor by a Lipophilic Small Molecule.
  Biochemistry 2018 Bokoch MP, Jo H, Valcourt JR, Srinivasan Y, Pan AC, Capponi S, Grabe M, Dror RO, Shaw DE, DeGrado WF, Coughlin SR

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• A Rapid Method for Directed Gene Knockout for Screening in G0 Zebrafish.
  Developmental cell 2018 Wu RS, Lam II, Clay H, Duong DN, Deo RC, Coughlin SR
• A rapid fluorogenic GPCR-β-arrestin interaction assay.
  Protein science : a publication of the Protein Society 2018 Zhang Q, Zheng YW, Coughlin SR, Shu X
• Visualizing Dynamics of Cell Signaling In Vivo with a Phase Separation-Based Kinase Reporter.
  Molecular cell 2018 Zhang Q, Huang H, Zhang L, Wu R, Chung CI, Zhang SQ, Torra J, Schepis A, Coughlin SR, Kornberg TB, Shu X
• Inactivating mutations in Drosha mediate vascular abnormalities similar to hereditary hemorrhagic telangiectasia.
  Science signaling 2018 Jiang X, Wooderchak-Donahue WL, McDonald J, Ghatpande P, Baalbaki M, Sandoval M, Hart D, Clay H, Coughlin S, Lagna G, Bayrak-Toydemir P, Hata A
• Structural Basis for Activity and Specificity of an Anticoagulant Anti-FXIa Monoclonal Antibody and a Reversal Agent.
  Structure (London, England : 1993) 2018 Ely LK, Lolicato M, David T, Lowe K, Kim YC, Samuel D, Bessette P, Garcia JL, Mikita T, Minor DL, Coughlin SR
• Protease signaling regulates apical cell extrusion, cell contacts, and proliferation in epithelia.
  The Journal of cell biology 2018 Schepis A, Barker A, Srinivasan Y, Balouch E, Zheng Y, Lam I, Clay H, Hsiao CD, Coughlin SR
• Visualizing Dynamics of Cell Signaling In Vivo with a Phase Separation-Based Kinase Reporter.
  Molecular cell 2018 Zhang Q, Huang H, Zhang L, Wu R, Chung CI, Zhang SQ, Torra J, Schepis A, Coughlin SR, Kornberg TB, Shu X
• The lung is a site of platelet biogenesis and a reservoir for haematopoietic progenitors.
  Nature 2017 Lefrançais E, Ortiz-Muñoz G, Caudrillier A, Mallavia B, Liu F, Sayah DM, Thornton EE, Headley MB, David T, Coughlin SR, Krummel MF, Leavitt AD, Passegué E, Looney MR
• Platelet and Erythrocyte Sources of S1P Are Redundant for Vascular Development and Homeostasis, but Both Rendered Essential After Plasma S1P Depletion in Anaphylactic Shock.
  Circulation research 2016 Gazit SL, Mariko B, Thérond P, Decouture B, Xiong Y, Couty L, Bonnin P, Baudrie V, Le Gall SM, Dizier B, Zoghdani N, Ransinan J, Hamilton JR, Gaussem P, Tharaux PL, Chun J, Coughlin SR, Bachelot-Loza …
• Factor XIa-specific IgG and a reversal agent to probe factor XI function in thrombosis and hemostasis.
  Science translational medicine 2016 David T, Kim YC, Ely LK, Rondon I, Gao H, O'Brien P, Bolt MW, Coyle AJ, Garcia JL, Flounders EA, Mikita T, Coughlin SR
• Rational Design of a GFP-Based Fluorogenic Caspase Reporter for Imaging Apoptosis In Vivo.
  Cell chemical biology 2016 To TL, Schepis A, Ruiz-González R, Zhang Q, Yu D, Dong Z, Coughlin SR, Shu X
• Sphingosine 1-phosphate receptor-1 in cardiomyocytes is required for normal cardiac development.
  Developmental biology 2016 Clay H, Wilsbacher LD, Wilson SJ, Duong DN, McDonald M, Lam I, Park KE, Chun J, Coughlin SR
• A mutation in the atrial-specific myosin light chain gene (MYL4) causes familial atrial fibrillation.
  Nature communications 2016 Orr N, Arnaout R, Gula LJ, Spears DA, Leong-Sit P, Li Q, Tarhuni W, Reischauer S, Chauhan VS, Borkovich M, Uppal S, Adler A, Coughlin SR, Stainier DYR, Gollob MH
• Identifying and quantifying two ligand-binding sites while imaging native human membrane receptors by AFM.
  Nature communications 2015 Pfreundschuh M, Alsteens D, Wieneke R, Zhang C, Coughlin SR, Tampé R, Kobilka BK, Müller DJ
• An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish.
  eLife 2015 Zou J, Tran D, Baalbaki M, Tang LF, Poon A, Pelonero A, Titus EW, Yuan C, Shi C, Patchava S, Halper E, Garg J, Movsesyan I, Yin C, Wu R, Wilsbacher LD, Liu J, Hager RL, Coughlin SR, Jinek M, Pullinger…
• Non-invasive imaging and cellular tracking of pulmonary emboli by near-infrared fluorescence and positron-emission tomography.
  Nature communications 2015 Page MJ, Lourenço AL, David T, LeBeau AM, Cattaruzza F, Castro HC, VanBrocklin HF, Coughlin SR, Craik CS
• Mice expressing a mutant form of fibrinogen that cannot support fibrin formation exhibit compromised antimicrobial host defense.
  Blood 2015 Prasad JM, Gorkun OV, Raghu H, Thornton S, Mullins ES, Palumbo JS, Ko YP, Höök M, David T, Coughlin SR, Degen JL, Flick MJ
• Imaging G protein-coupled receptors while quantifying their ligand-binding free-energy landscape.
  Nature methods 2015 Alsteens D, Pfreundschuh M, Zhang C, Spoerri PM, Coughlin SR, Kobilka BK, Müller DJ
• Analysis of cardiomyocyte development using immunofluorescence in embryonic mouse heart.
  Journal of visualized experiments : JoVE 2015 Wilsbacher LD, Coughlin SR
• Gαi/o-coupled receptor signaling restricts pancreatic β-cell expansion.
  Proceedings of the National Academy of Sciences of the United States of America 2015 Berger M, Scheel DW, Macias H, Miyatsuka T, Kim H, Hoang P, Ku GM, Honig G, Liou A, Tang Y, Regard JB, Sharifnia P, Yu L, Wang J, Coughlin SR, Conklin BR, Deneris ES, Tecott LH, German MS
• Mechanical vessel injury in zebrafish embryos.
  Journal of visualized experiments : JoVE 2015 Clay H, Coughlin SR
• Common variants in the human platelet PAR4 thrombin receptor alter platelet function and differ by race.
  Blood 2014 Edelstein LC, Simon LM, Lindsay CR, Kong X, Teruel-Montoya R, Tourdot BE, Chen ES, Ma L, Coughlin S, Nieman M, Holinstat M, Shaw CA, Bray PF
• Redundancy and interaction of thrombin- and collagen-mediated platelet activation in tail bleeding and carotid thrombosis in mice.
  Arteriosclerosis, thrombosis, and vascular biology 2014 Bynagari-Settipalli YS, Cornelissen I, Palmer D, Duong D, Concengco C, Ware J, Coughlin SR
• The imperative to invest in science has never been greater.
  The Journal of clinical investigation 2014 Carethers JM, Coughlin S, Diamond B, Erzurum S, Fried LP, Jameson JL, Kaushansky K, Klotman ME, Lemon S, Mitchell B, Rothman P, Sawyers C, Seidman C, Somlo S
• Podoplanin maintains high endothelial venule integrity by interacting with platelet CLEC-2.
  Nature 2013 Herzog BH, Fu J, Wilson SJ, Hess PR, Sen A, McDaniel JM, Pan Y, Sheng M, Yago T, Silasi-Mansat R, McGee S, May F, Nieswandt B, Morris AJ, Lupu F, Coughlin SR, McEver RP, Chen H, Kahn ML, Xia L
• IL-7 production in murine lymphatic endothelial cells and induction in the setting of peripheral lymphopenia.
  International immunology 2013 Miller CN, Hartigan-O'Connor DJ, Lee MS, Laidlaw G, Cornelissen IP, Matloubian M, Coughlin SR, McDonald DM, McCune JM
• High-resolution crystal structure of human protease-activated receptor 1.
  Nature 2012 Zhang C, Srinivasan Y, Arlow DH, Fung JJ, Palmer D, Zheng Y, Green HF, Pandey A, Dror RO, Shaw DE, Weis WI, Coughlin SR, Kobilka BK
• PAR1 contributes to influenza A virus pathogenicity in mice.
  The Journal of clinical investigation 2012 Khoufache K, Berri F, Nacken W, Vogel AB, Delenne M, Camerer E, Coughlin SR, Carmeliet P, Lina B, Rimmelzwaan GF, Planz O, Ludwig S, Riteau B
• TMEM16F forms a Ca2+-activated cation channel required for lipid scrambling in platelets during blood coagulation.
  Cell 2012 Yang H, Kim A, David T, Palmer D, Jin T, Tien J, Huang F, Cheng T, Coughlin SR, Jan YN, Jan LY
• Canadian Surgery Forum.
  Canadian journal of surgery. Journal canadien de chirurgie 2012 Fayez R, Roy M, Villeneuve S, AlMuntashery A, Demyttenaere S, Christou N, Court O, AlMuntashery A, Fayez R, Demyttenaere S, Christou N, Court O, Bodie G, Bonrath E, Hagen J, Okrainec A, Sullivan P, …
• Wnt/β-catenin signaling is differentially regulated by Gα proteins and contributes to fibrous dysplasia.
  Proceedings of the National Academy of Sciences of the United States of America 2011 Regard JB, Cherman N, Palmer D, Kuznetsov SA, Celi FS, Guettier JM, Chen M, Bhattacharyya N, Wess J, Coughlin SR, Weinstein LS, Collins MT, Robey PG, Yang Y
• GRK2-dependent S1PR1 desensitization is required for lymphocytes to overcome their attraction to blood.
  Science (New York, N.Y.) 2011 Arnon TI, Xu Y, Lo C, Pham T, An J, Coughlin S, Dorn GW, Cyster JG
• Carcinoma mucins trigger reciprocal activation of platelets and neutrophils in a murine model of Trousseau syndrome.
  Blood 2011 Shao B, Wahrenbrock MG, Yao L, David T, Coughlin SR, Xia L, Varki A, McEver RP
• Canadian Surgery Forum: Abstracts of presentations to the Annual Meetings of the Canadian Association of Bariatric Physicians and Surgeons, Canadian Association of General Surgeons, Canadian Association of Thoracic Surgeons, Canadian Hepato-Pancreato-Biliary Society, Canadian Society of Surgical Oncology, Canadian Society of Colon and Rectal Surgeons, London, Ont. Sept. 15-18, 2011.
  Canadian journal of surgery. Journal canadien de chirurgie 2011 Chiu JC, Shi X, Karmali S, Birch DW, Apriasz I, Alkhamesi NA, Lal A, Schlachta CM, Christou NV, Elkassem S, Lindsay D, Smith L, Sullivan P, Sockalingam S, Hawa R, Wnuk S, Jackson T, Okrainec A, Fayez …
• The sphingosine 1-phosphate receptor S1P₂ maintains the homeostasis of germinal center B cells and promotes niche confinement.
  Nature immunology 2011 Green JA, Suzuki K, Cho B, Willison LD, Palmer D, Allen CD, Schmidt TH, Xu Y, Proia RL, Coughlin SR, Cyster JG
• PAR2 absence completely rescues inflammation and ichthyosis caused by altered CAP1/Prss8 expression in mouse skin.
  Nature communications 2011 Frateschi S, Camerer E, Crisante G, Rieser S, Membrez M, Charles RP, Beermann F, Stehle JC, Breiden B, Sandhoff K, Rotman S, Haftek M, Wilson A, Ryser S, Steinhoff M, Coughlin SR, Hummler E
• Roles and interactions among protease-activated receptors and P2ry12 in hemostasis and thrombosis.
  Proceedings of the National Academy of Sciences of the United States of America 2010 Cornelissen I, Palmer D, David T, Wilsbacher L, Concengco C, Conley P, Pandey A, Coughlin SR
• Canadian Surgery Forum.
  Canadian journal of surgery. Journal canadien de chirurgie 2010 Atlas H, Safa N, Denis R, Garneau P, Moustarah F, Marceau S, Lebel S, Biertho L, Hould F, Marceau P, Biron S, Anvari M, Sharma A, Goldsmith CH, Lacobellis G, Cadeddu M, Misra M, Taylor V, Tarride J, …
• Identifying patients at high risk of a cardiovascular event in the near future: current status and future directions: report of a national heart, lung, and blood institute working group.
  Circulation 2010 Eagle KA, Ginsburg GS, Musunuru K, Aird WC, Balaban RS, Bennett SK, Blumenthal RS, Coughlin SR, Davidson KW, Frohlich ED, Greenland P, Jarvik GP, Libby P, Pepine CJ, Ruskin JN, Stillman AE, Van Eyk JE…
• Local protease signaling contributes to neural tube closure in the mouse embryo.
  Developmental cell 2010 Camerer E, Barker A, Duong DN, Ganesan R, Kataoka H, Cornelissen I, Darragh MR, Hussain A, Zheng YW, Srinivasan Y, Brown C, Xu SM, Regard JB, Lin CY, Craik CS, Kirchhofer D, Coughlin SR
• Lymphatic endothelial cell sphingosine kinase activity is required for lymphocyte egress and lymphatic patterning.
  The Journal of experimental medicine 2009 Pham TH, Baluk P, Xu Y, Grigorova I, Bankovich AJ, Pappu R, Coughlin SR, McDonald DM, Schwab SR, Cyster JG
• Abstracts of presentations to the Annual Meetings of the Canadian Association of General Surgeons Canadian Association of Thoracic Surgeons Canadian Hepato-Pancreato-Biliary Society Canadian Society of Surgical Oncology Canadian Society of Colon and Rectal Surgeons: Victoria, BC Sept. 10-13, 2009.
  Canadian journal of surgery. Journal canadien de chirurgie 2009 Nenshi R, Kennedy E, Baxter NN, Saskin R, Sutradhar R, Urbach DR, Sroka G, Feldman LS, Vassiliou MC, Kaneva PA, Fayez R, Fried GM, Krajewski SA, Brown CJ, Hur C, McCrea PH, Mitchell A, Porter G, …
• Sphingosine-1-phosphate in the plasma compartment regulates basal and inflammation-induced vascular leak in mice.
  The Journal of clinical investigation 2009 Camerer E, Regard JB, Cornelissen I, Srinivasan Y, Duong DN, Palmer D, Pham TH, Wong JS, Pappu R, Coughlin SR
• Atherosclerosis proceeds independently of thrombin-induced platelet activation in ApoE-/- mice.
  Atherosclerosis 2009 Hamilton JR, Cornelissen I, Mountford JK, Coughlin SR
• Identification of a transient subpial neurogenic zone in the developing dentate gyrus and its regulation by Cxcl12 and reelin signaling.
  Development (Cambridge, England) 2009 Li G, Kataoka H, Coughlin SR, Pleasure SJ
• Anatomical profiling of G protein-coupled receptor expression.
  Cell 2008 Regard JB, Sato IT, Coughlin SR
• Mechanisms and consequences of agonist-induced talin recruitment to platelet integrin alphaIIbbeta3.
  The Journal of cell biology 2008 Watanabe N, Bodin L, Pandey M, Krause M, Coughlin S, Boussiotis VA, Ginsberg MH, Shattil SJ
• Maternal Par4 and platelets contribute to defective placenta formation in mouse embryos lacking thrombomodulin.
  Blood 2008 Sood R, Sholl L, Isermann B, Zogg M, Coughlin SR, Weiler H
• A critical role for extracellular protein disulfide isomerase during thrombus formation in mice.
  The Journal of clinical investigation 2008 Cho J, Furie BC, Coughlin SR, Furie B
• Probing cell type-specific functions of Gi in vivo identifies GPCR regulators of insulin secretion.
  The Journal of clinical investigation 2007 Regard JB, Kataoka H, Cano DA, Camerer E, Yin L, Zheng YW, Scanlan TS, Hebrok M, Coughlin SR
• Fetal gene defects precipitate platelet-mediated pregnancy failure in factor V Leiden mothers.
  The Journal of experimental medicine 2007 Sood R, Zogg M, Westrick RJ, Guo YH, Kerschen EJ, Girardi G, Salmon JE, Coughlin SR, Weiler H
• Protease-activated receptor 2, dipeptidyl peptidase I, and proteases mediate Clostridium difficile toxin A enteritis.
  Gastroenterology 2007 Cottrell GS, Amadesi S, Pikios S, Camerer E, Willardsen JA, Murphy BR, Caughey GH, Wolters PJ, Coughlin SR, Peterson A, Knecht W, Pothoulakis C, Bunnett NW, Grady EF
• Promotion of lymphocyte egress into blood and lymph by distinct sources of sphingosine-1-phosphate.
  Science (New York, N.Y.) 2007 Pappu R, Schwab SR, Cornelissen I, Pereira JP, Regard JB, Xu Y, Camerer E, Zheng YW, Huang Y, Cyster JG, Coughlin SR
• Par4 is required for platelet thrombus propagation but not fibrin generation in a mouse model of thrombosis.
  Proceedings of the National Academy of Sciences of the United States of America 2006 Vandendries ER, Hamilton JR, Coughlin SR, Furie B, Furie BC
• Defective platelet responsiveness to thrombin and protease-activated receptors agonists in a novel case of gray platelet syndrome: correlation between the platelet defect and the alpha-granule content in the patient and four relatives.
  Journal of thrombosis and haemostasis : JTH 2006 De Candia E, Pecci A, Ciabattoni G, De Cristofaro R, Rutella S, Yao-Wu Z, Lazzareschi I, Landolfi R, Coughlin S, Balduini CL
• Inhibition of coxsackievirus B3 in cell cultures and in mice by peptide-conjugated morpholino oligomers targeting the internal ribosome entry site.
  Journal of virology 2006 Yuan J, Stein DA, Lim T, Qiu D, Coughlin S, Liu Z, Wang Y, Blouch R, Moulton HM, Iversen PL, Yang D
• Roles of protease-activated receptors in a mouse model of endotoxemia.
  Blood 2006 Camerer E, Cornelissen I, Kataoka H, Duong DN, Zheng YW, Coughlin SR
• Protease-activated receptor-2 activation induces acute lung inflammation by neuropeptide-dependent mechanisms.
  Journal of immunology (Baltimore, Md. : 1950) 2005 Su X, Camerer E, Hamilton JR, Coughlin SR, Matthay MA
• Protease-activated receptors in hemostasis, thrombosis and vascular biology.
  Journal of thrombosis and haemostasis : JTH 2005 Coughlin SR
• Essential role for Galpha13 in endothelial cells during embryonic development.
  Proceedings of the National Academy of Sciences of the United States of America 2005 Ruppel KM, Willison D, Kataoka H, Wang A, Zheng YW, Cornelissen I, Yin L, Xu SM, Coughlin SR
• PAR1 cleavage and signaling in response to activated protein C and thrombin.
  The Journal of biological chemistry 2005 Ludeman MJ, Kataoka H, Srinivasan Y, Esmon NL, Esmon CT, Coughlin SR
• Impaired hemostasis and protection against thrombosis in protease-activated receptor 4-deficient mice is due to lack of thrombin signaling in platelets.
  Journal of thrombosis and haemostasis : JTH 2004 Hamilton JR, Cornelissen I, Coughlin SR
• Platelets, protease-activated receptors, and fibrinogen in hematogenous metastasis.
  Blood 2004 Camerer E, Qazi AA, Duong DN, Cornelissen I, Advincula R, Coughlin SR
• Regulated shedding of PAR1 N-terminal exodomain from endothelial cells.
  The Journal of biological chemistry 2004 Ludeman MJ, Zheng YW, Ishii K, Coughlin SR
• Combined deficiency of protease-activated receptor-4 and fibrinogen recapitulates the hemostatic defect but not the embryonic lethality of prothrombin deficiency.
  Blood 2003 Camerer E, Duong DN, Hamilton JR, Coughlin SR
• Protease-activated receptors 1 and 4 mediate thrombin signaling in endothelial cells.
  Blood 2003 Kataoka H, Hamilton JR, McKemy DD, Camerer E, Zheng YW, Cheng A, Griffin C, Coughlin SR
• The Rho guanine nucleotide exchange factor Lsc homo-oligomerizes and is negatively regulated through domains in its carboxyl terminus that are absent in novel splenic isoforms.
  The Journal of biological chemistry 2003 Eisenhaure TM, Francis SA, Willison LD, Coughlin SR, Lerner DJ
• PARticipation in inflammation.
  The Journal of clinical investigation 2003 Coughlin SR, Camerer E
• Overview of the Alliance for Cellular Signaling.
  Nature 2002 Gilman AG, Simon MI, Bourne HR, Harris BA, Long R, Ross EM, Stull JT, Taussig R, Bourne HR, Arkin AP, Cobb MH, Cyster JG, Devreotes PN, Ferrell JE, Fruman D, Gold M, Weiss A, Stull JT, Berridge MJ, …
• Protection against thrombosis in mice lacking PAR3.
  Blood 2002 Weiss EJ, Hamilton JR, Lease KE, Coughlin SR
• Genetic evidence that protease-activated receptors mediate factor Xa signaling in endothelial cells.
  The Journal of biological chemistry 2002 Camerer E, Kataoka H, Kahn M, Lease K, Coughlin SR
• Protease-activated receptors in the cardiovascular system.
  Cold Spring Harbor symposia on quantitative biology 2002 Coughlin SR
• Role of thrombin signalling in platelets in haemostasis and thrombosis.
  Nature 2001 Sambrano GR, Weiss EJ, Zheng YW, Huang W, Coughlin SR
• A role for thrombin receptor signaling in endothelial cells during embryonic development.
  Science (New York, N.Y.) 2001 Griffin CT, Srinivasan Y, Zheng YW, Huang W, Coughlin SR
• Protease-activated receptors in vascular biology.
  Thrombosis and haemostasis 2001 Coughlin SR
• Delayed onset of inflammation in protease-activated receptor-2-deficient mice.
  Journal of immunology (Baltimore, Md. : 1950) 2000 Lindner JR, Kahn ML, Coughlin SR, Sambrano GR, Schauble E, Bernstein D, Foy D, Hafezi-Moghadam A, Ley K
• Protease-activated receptor-1 down-regulation: a mutant HeLa cell line suggests novel requirements for PAR1 phosphorylation and recruitment to clathrin-coated pits.
  The Journal of biological chemistry 2000 Trejo J, Altschuler Y, Fu HW, Mostov KE, Coughlin SR
• Thrombin signalling and protease-activated receptors.
  Nature 2000 Coughlin SR
• Cellular localization of membrane-type serine protease 1 and identification of protease-activated receptor-2 and single-chain urokinase-type plasminogen activator as substrates.
  The Journal of biological chemistry 2000 Takeuchi T, Harris JL, Huang W, Yan KW, Coughlin SR, Craik CS
• Protease-activated receptors 1 and 4 are shut off with distinct kinetics after activation by thrombin.
  The Journal of biological chemistry 2000 Shapiro MJ, Weiss EJ, Faruqi TR, Coughlin SR
• Structure-function analysis of protease-activated receptor 4 tethered ligand peptides. Determinants of specificity and utility in assays of receptor function.
  The Journal of biological chemistry 2000 Faruqi TR, Weiss EJ, Shapiro MJ, Huang W, Coughlin SR
• Tissue factor- and factor X-dependent activation of protease-activated receptor 2 by factor VIIa.
  Proceedings of the National Academy of Sciences of the United States of America 2000 Camerer E, Huang W, Coughlin SR
• Prospective randomized trial of docetaxel versus best supportive care in patients with non-small-cell lung cancer previously treated with platinum-based chemotherapy.
  Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2000 Shepherd FA, Dancey J, Ramlau R, Mattson K, Gralla R, O'Rourke M, Levitan N, Gressot L, Vincent M, Burkes R, Coughlin S, Kim Y, Berille J
• PAR3 is a cofactor for PAR4 activation by thrombin.
  Nature 2000 Nakanishi-Matsui M, Zheng YW, Sulciner DJ, Weiss EJ, Ludeman MJ, Coughlin SR
• Cathepsin G activates protease-activated receptor-4 in human platelets.
  The Journal of biological chemistry 2000 Sambrano GR, Huang W, Faruqi T, Mahrus S, Craik C, Coughlin SR
• Protease-activated receptor 1 mediates thrombin-dependent, cell-mediated renal inflammation in crescentic glomerulonephritis.
  The Journal of experimental medicine 2000 Cunningham MA, Rondeau E, Chen X, Coughlin SR, Holdsworth SR, Tipping PG
• Glycoprotein V-deficient platelets have undiminished thrombin responsiveness and Do not exhibit a Bernard-Soulier phenotype.
  Blood 1999 Kahn ML, Diacovo TG, Bainton DF, Lanza F, Trejo J, Coughlin SR
• How the protease thrombin talks to cells.
  Proceedings of the National Academy of Sciences of the United States of America 1999 Coughlin SR
• Protease-activated receptors and platelet function.
  Thrombosis and haemostasis 1999 Coughlin SR
• Shutoff and agonist-triggered internalization of protease-activated receptor 1 can be separated by mutation of putative phosphorylation sites in the cytoplasmic tail.
  Biochemistry 1999 Hammes SR, Shapiro MJ, Coughlin SR
• The carboxyl tail of protease-activated receptor-1 is required for chemotaxis. Correlation of signal termination and directional migration.
  The Journal of biological chemistry 1999 Sambrano GR, Coughlin SR
• Protease-activated receptors 1 and 4 mediate activation of human platelets by thrombin.
  The Journal of clinical investigation 1999 Kahn ML, Nakanishi-Matsui M, Shapiro MJ, Ishihara H, Coughlin SR
• Protease-activated receptor-1 can mediate responses to SFLLRN in thrombin-desensitized cells: evidence for a novel mechanism for preventing or terminating signaling by PAR1's tethered ligand.
  Biochemistry 1999 Hammes SR, Coughlin SR
• The cytoplasmic tails of protease-activated receptor-1 and substance P receptor specify sorting to lysosomes versus recycling.
  The Journal of biological chemistry 1999 Trejo J, Coughlin SR
• Termination of signaling by protease-activated receptor-1 is linked to lysosomal sorting.
  Proceedings of the National Academy of Sciences of the United States of America 1998 Trejo J, Hammes SR, Coughlin SR
• Separate signals for agonist-independent and agonist-triggered trafficking of protease-activated receptor 1.
  The Journal of biological chemistry 1998 Shapiro MJ, Coughlin SR
• Gene and locus structure and chromosomal localization of the protease-activated receptor gene family.
  The Journal of biological chemistry 1998 Kahn ML, Hammes SR, Botka C, Coughlin SR
• A dual thrombin receptor system for platelet activation.
  Nature 1998 Kahn ML, Zheng YW, Huang W, Bigornia V, Zeng D, Moff S, Farese RV, Tam C, Coughlin SR
• Antibodies to protease-activated receptor 3 inhibit activation of mouse platelets by thrombin.
  Blood 1998 Ishihara H, Zeng D, Connolly AJ, Tam C, Coughlin SR
• Sol Sherry lecture in thrombosis: how thrombin 'talks' to cells: molecular mechanisms and roles in vivo.
  Arteriosclerosis, thrombosis, and vascular biology 1998 Coughlin SR
• A mammalian homolog of fission yeast Cdc5 regulates G2 progression and mitotic entry.
  The Journal of biological chemistry 1998 Bernstein HS, Coughlin SR
• Identification of cDNAs encoding two G protein-coupled receptors for lysosphingolipids.
  FEBS letters 1997 An S, Bleu T, Huang W, Hallmark OG, Coughlin SR, Goetzl EJ
• Mice lacking the thrombin receptor, PAR1, have normal skin wound healing.
  The American journal of pathology 1997 Connolly AJ, Suh DY, Hunt TK, Coughlin SR
• Protease-activated receptor 3 is a second thrombin receptor in humans.
  Nature 1997 Ishihara H, Connolly AJ, Zeng D, Kahn ML, Zheng YW, Timmons C, Tram T, Coughlin SR
• The thrombin receptor second cytoplasmic loop confers coupling to Gq-like G proteins in chimeric receptors. Additional evidence for a common transmembrane signaling and G protein coupling mechanism in G protein-coupled receptors.
  The Journal of biological chemistry 1997 Verrall S, Ishii M, Chen M, Wang L, Tram T, Coughlin SR
• Pombe Cdc5-related protein. A putative human transcription factor implicated in mitogen-activated signaling.
  The Journal of biological chemistry 1997 Bernstein HS, Coughlin SR
• Thrombin receptors on human platelets. Initial localization and subsequent redistribution during platelet activation.
  The Journal of biological chemistry 1997 Molino M, Bainton DF, Hoxie JA, Coughlin SR, Brass LF
• Role of the thrombin receptor's cytoplasmic tail in intracellular trafficking. Distinct determinants for agonist-triggered versus tonic internalization and intracellular localization.
  The Journal of biological chemistry 1996 Shapiro MJ, Trejo J, Zeng D, Coughlin SR
• Phosphorylation by a G protein-coupled kinase inhibits signaling and promotes internalization of the monocyte chemoattractant protein-1 receptor. Critical role of carboxyl-tail serines/threonines in receptor function.
  Journal of immunology (Baltimore, Md. : 1950) 1996 Franci C, Gosling J, Tsou CL, Coughlin SR, Charo IF
• The cloned thrombin receptor is necessary and sufficient for activation of mitogen-activated protein kinase and mitogenesis in mouse lung fibroblasts. Loss of responses in fibroblasts from receptor knockout mice.
  The Journal of biological chemistry 1996 Trejo J, Connolly AJ, Coughlin SR
• Thrombin potently stimulates cytokine production in human vascular smooth muscle cells but not in mononuclear phagocytes.
  Circulation research 1996 Kranzhöfer R, Clinton SK, Ishii K, Coughlin SR, Fenton JW, Libby P
• Agonist recognition by proteinase-activated receptor 2 and thrombin receptor. Importance of extracellular loop interactions for receptor function.
  The Journal of biological chemistry 1996 Lerner DJ, Chen M, Tram T, Coughlin SR
• Role of the thrombin receptor in development and evidence for a second receptor.
  Nature 1996 Connolly AJ, Ishihara H, Kahn ML, Farese RV, Coughlin SR
• Conserved structure and adjacent location of the thrombin receptor and protease-activated receptor 2 genes define a protease-activated receptor gene cluster.
  Molecular medicine (Cambridge, Mass.) 1996 Kahn M, Ishii K, Kuo WL, Piper M, Connolly A, Shi YP, Wu R, Lin CC, Coughlin SR
• Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway.
  Current biology : CB 1996 Brown JL, Stowers L, Baer M, Trejo J, Coughlin S, Chant J
• Molecular cloning, expression and potential functions of the human proteinase-activated receptor-2.
  The Biochemical journal 1996 Bohm SK, Kong W, Bromme D, Smeekens SP, Anderson DC, Connolly A, Kahn M, Nelken NA, Coughlin SR, Payan DG, Bunnett NW
• Thrombin receptor activating mutations. Alteration of an extracellular agonist recognition domain causes constitutive signaling.
  The Journal of biological chemistry 1996 Nanevicz T, Wang L, Chen M, Ishii M, Coughlin SR
• Tethered ligand library for discovery of peptide agonists.
  The Journal of biological chemistry 1995 Chen J, Bernstein HS, Chen M, Wang L, Ishii M, Turck CW, Coughlin SR
• Mechanisms of thrombin receptor agonist specificity. Chimeric receptors and complementary mutations identify an agonist recognition site.
  The Journal of biological chemistry 1995 Nanevicz T, Ishii M, Wang L, Chen M, Chen J, Turck CW, Cohen FE, Coughlin SR
• Determinants of thrombin receptor cleavage. Receptor domains involved, specificity, and role of the P3 aspartate.
  The Journal of biological chemistry 1995 Ishii K, Gerszten R, Zheng YW, Welsh JB, Turck CW, Coughlin SR
• Intracellular targeting and trafficking of thrombin receptors. A novel mechanism for resensitization of a G protein-coupled receptor.
  The Journal of biological chemistry 1994 Hein L, Ishii K, Coughlin SR, Kobilka BK
• Molecular mechanisms of thrombin signaling.
  Seminars in hematology 1994 Coughlin SR
• Protease-activated receptors start a family.
  Proceedings of the National Academy of Sciences of the United States of America 1994 Coughlin SR
• Thrombin receptor activation. Confirmation of the intramolecular tethered liganding hypothesis and discovery of an alternative intermolecular liganding mode.
  The Journal of biological chemistry 1994 Chen J, Ishii M, Wang L, Ishii K, Coughlin SR
• Specificity of the thrombin receptor for agonist peptide is defined by its extracellular surface.
  Nature 1994 Gerszten RE, Chen J, Ishii M, Ishii K, Wang L, Nanevicz T, Turck CW, Vu TK, Coughlin SR
• Expanding horizons for receptors coupled to G proteins: diversity and disease.
  Current opinion in cell biology 1994 Coughlin SR
• Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternative splicing of the carboxyl-terminal tails.
  Proceedings of the National Academy of Sciences of the United States of America 1994 Charo IF, Myers SJ, Herman A, Franci C, Connolly AJ, Coughlin SR
• Crystallographic structures of thrombin complexed with thrombin receptor peptides: existence of expected and novel binding modes.
  Biochemistry 1994 Mathews II, Padmanabhan KP, Ganesh V, Tulinsky A, Ishii M, Chen J, Turck CW, Coughlin SR, Fenton JW
• Thrombin receptor function and cardiovascular disease.
  Trends in cardiovascular medicine 1994 Coughlin SR
• Inhibition of thrombin receptor signaling by a G-protein coupled receptor kinase. Functional specificity among G-protein coupled receptor kinases.
  The Journal of biological chemistry 1994 Ishii K, Chen J, Ishii M, Koch WJ, Freedman NJ, Lefkowitz RJ, Coughlin SR
• Disparate temporal expression of the prothrombin and thrombin receptor genes during mouse development.
  The American journal of pathology 1994 Soifer SJ, Peters KG, O'Keefe J, Coughlin SR
• Thrombin receptor structure and function.
  Thrombosis and haemostasis 1993 Coughlin SR
• Kinetics of thrombin receptor cleavage on intact cells. Relation to signaling.
  The Journal of biological chemistry 1993 Ishii K, Hein L, Kobilka B, Coughlin SR
• Thrombin stimulates proliferation of cultured rat aortic smooth muscle cells by a proteolytically activated receptor.
  The Journal of clinical investigation 1993 McNamara CA, Sarembock IJ, Gimple LW, Fenton JW, Coughlin SR, Owens GK
• The cloned platelet thrombin receptor couples to at least two distinct effectors to stimulate phosphoinositide hydrolysis and inhibit adenylyl cyclase.
  The Journal of biological chemistry 1992 Hung DT, Wong YH, Vu TK, Coughlin SR
• Thrombin receptor expression in normal and atherosclerotic human arteries.
  The Journal of clinical investigation 1992 Nelken NA, Soifer SJ, O'Keefe J, Vu TK, Charo IF, Coughlin SR
• Tethered ligand agonist peptides. Structural requirements for thrombin receptor activation reveal mechanism of proteolytic unmasking of agonist function.
  The Journal of biological chemistry 1992 Scarborough RM, Naughton MA, Teng W, Hung DT, Rose J, Vu TK, Wheaton VI, Turck CW, Coughlin SR
• Cloned platelet thrombin receptor is necessary for thrombin-induced platelet activation.
  The Journal of clinical investigation 1992 Hung DT, Vu TK, Wheaton VI, Ishii K, Coughlin SR
• "Mirror image" antagonists of thrombin-induced platelet activation based on thrombin receptor structure.
  The Journal of clinical investigation 1992 Hung DT, Vu TK, Wheaton VI, Charo IF, Nelken NA, Esmon N, Esmon CT, Coughlin SR
• Characterization of a functional thrombin receptor. Issues and opportunities.
  The Journal of clinical investigation 1992 Coughlin SR, Vu TK, Hung DT, Wheaton VI
• Thrombin-induced events in non-platelet cells are mediated by the unique proteolytic mechanism established for the cloned platelet thrombin receptor.
  The Journal of cell biology 1992 Hung DT, Vu TH, Nelken NA, Coughlin SR
• Expression cloning and characterization of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation.
  Seminars in thrombosis and hemostasis 1992 Coughlin SR, Vu TK, Hung DT, Wheaton VI
• Thrombin receptor structure and function.
  Cold Spring Harbor symposia on quantitative biology 1992 Coughlin SR, Scarborough RM, Vu TK, Hung DT
• Domains specifying thrombin-receptor interaction.
  Nature 1991 Vu TK, Wheaton VI, Hung DT, Charo I, Coughlin SR
• Monocyte chemoattractant protein-1 in human atheromatous plaques.
  The Journal of clinical investigation 1991 Nelken NA, Coughlin SR, Gordon D, Wilcox JN
• The region of the thrombin receptor resembling hirudin binds to thrombin and alters enzyme specificity.
  The Journal of biological chemistry 1991 Liu LW, Vu TK, Esmon CT, Coughlin SR
• cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane-spanning proteins.
  The Journal of biological chemistry 1991 Lanza F, Wolf D, Fox CF, Kieffer N, Seyer JM, Fried VA, Coughlin SR, Phillips DR, Jennings LK
• Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation.
  Cell 1991 Vu TK, Hung DT, Wheaton VI, Coughlin SR
• Role of phosphatidylinositol kinase in PDGF receptor signal transduction.
  Science (New York, N.Y.) 1989 Coughlin SR, Escobedo JA, Williams LT
• Serotonin-induced deoxyribonucleic acid synthesis in vascular smooth muscle cells involves a novel, pertussis toxin-sensitive pathway.
  Molecular endocrinology (Baltimore, Md.) 1988 Kavanaugh WM, Williams LT, Ives HE, Coughlin SR
• A common PDGF receptor is activated by homodimeric A and B forms of PDGF.
  Science (New York, N.Y.) 1988 Escobedo JA, Navankasatussas S, Cousens LS, Coughlin SR, Bell GI, Williams LT
• Acidic and basic fibroblast growth factors stimulate tyrosine kinase activity in vivo.
  The Journal of biological chemistry 1988 Coughlin SR, Barr PJ, Cousens LS, Fretto LJ, Williams LT
• Arachidonic acid and cyclic adenosine monophosphate stimulation of c-fos expression by a pathway independent of phorbol ester-sensitive protein kinase C.
  Molecular endocrinology (Baltimore, Md.) 1988 Kacich RL, Williams LT, Coughlin SR
• Molecular mechanisms of platelet-derived growth factor action.
  Progress in clinical and biological research 1988 Coughlin SR, Escobedo JA, Williams LT
• Signal transduction by the platelet-derived growth factor receptor.
  Cold Spring Harbor symposia on quantitative biology 1988 Williams LT, Escobedo JA, Keating MT, Coughlin SR
• The stimulation of paracrine and autocrine mitogenic pathways by the platelet-derived growth factor receptor.
  Journal of cellular physiology. Supplement 1987 Williams LT, Escobedo JA, Keating MT, Coughlin SR
• Cardiac myocyte hypertrophy is associated with c-myc protooncogene expression.
  Proceedings of the National Academy of Sciences of the United States of America 1986 Starksen NF, Simpson PC, Bishopric N, Coughlin SR, Lee WM, Escobedo JA, Williams LT
• Dual trophic effects of the alpha 1-adrenergic receptor in cultured neonatal rat heart muscle cells.
  Journal of molecular and cellular cardiology 1986 Simpson P, Bishopric N, Coughlin S, Karliner J, Ordahl C, Starksen N, Tsao T, White N, Williams L
• c-myc gene expression is stimulated by agents that activate protein kinase C and does not account for the mitogenic effect of PDGF.
  Cell 1985 Coughlin SR, Lee WM, Williams PW, Giels GM, Williams LT
• Platelet-dependent stimulation of prostacyclin synthesis by platelet-derived growth factor.
  Nature 1980 Coughlin SR, Moskowitz MA, Zetter BR, Antoniades HN, Levine L