Richard Ward
Sr. Clinical Research Coord.
Medicine - Fresno
School of Medicine
Education & Training
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- Diversity, Equity, and Inclusion Champion Training University of California 2018
Publications (152)
Top publication keywords:
Protein EngineeringCrotalid VenomsPolygalacturonaseGlucansPhospholipases AEndo-1,4-beta XylanasesEnzyme Stabilitybeta-GalactosidaseProtein MultimerizationXylansEscherichia coliReceptors, G-Protein-CoupledBothropsGroup II Phospholipases A2Bacillus subtilis
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Microenzymes: Is There Anybody Out There?
The protein journal 2024 Salgado JCS, Alnoch RC, Polizeli MLTM, Ward RJ -
Mapping secondary substrate-binding sites on the GH11 xylanase from Bacillus subtilis.
FEBS letters 2024 Molina GA, Mendes LFS, Fuzo CA, Costa-Filho AJ, Ward RJ -
New animal model of chronic gout reproduces pathological features of the disease in humans.
RMD open 2023 Wang J, Hao P, Sun X, Ward R, Tang T, Chen X, Liu Y, Luo G, Yang Y, Xiang C, An S, Xu TR -
Xylose isomerase from Piromyces sp. E2 is a promiscuous enzyme with epimerase activity.
Enzyme and microbial technology 2023 Barreto MQ, Garbelotti CV, de Moura Soares J, Grandis A, Buckeridge MS, Leone FA, Ward RJ -
Biocatalytic potential of Pseudolycoriella CAZymes (Sciaroidea, Diptera) in degrading plant and fungal cell wall polysaccharides.
iScience 2023 Trinca V, Carli S, Uliana JVC, Garbelotti CV, Mendes da Silva M, Kunes V, Meleiro LP, Brancini GTP, Menzel F, Andrioli LPM, Torres TT, Ward RJ, Monesi N
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The Glycoside Hydrolase Family 35 β-galactosidase from Trichoderma reesei debranches xyloglucan oligosaccharides from tamarind and jatobá.
Biochimie 2023 Carneiro LABC, Wurman J, Dupree P, Ward RJ -
Quantification of Liver Iron Overload with MRI: Review and Guidelines from the ESGAR and SAR.
Radiology 2023 Reeder SB, Yokoo T, França M, Hernando D, Alberich-Bayarri Á, Alústiza JM, Gandon Y, Henninger B, Hillenbrand C, Jhaveri K, Karçaaltincaba M, Kühn JP, Mojtahed A, Serai SD, Ward R, Wood JC, Yamamura J… -
Glycomic profiling identifies key-structural differences in three arabinoxylan fractions from sugarcane culms.
Carbohydrate polymers 2023 Garbelotti CV, Grandis A, Crevelin E, Buckeridge MS, de Moraes LAB, Ward RJ -
The feruloyl esterase from Thermobacillus xylanilyticus shows broad specificity for processing pre-biotic feruloylated xylooligosaccharides at high temperatures.
Food chemistry 2022 Garbelotti CV, Bulmer GS, Ward RJ, van Munster JM -
The M1 muscarinic receptor is present in situ as a ligand-regulated mixture of monomers and oligomeric complexes.
Proceedings of the National Academy of Sciences of the United States of America 2022 Marsango S, Jenkins L, Pediani JD, Bradley SJ, Ward RJ, Hesse S, Biener G, Stoneman MR, Tobin AB, Raicu V, Milligan G -
Selective phosphorylation of threonine residues defines GPR84-arrestin interactions of biased ligands.
The Journal of biological chemistry 2022 Marsango S, Ward RJ, Jenkins L, Butcher AJ, Al Mahmud Z, Dwomoh L, Nagel F, Schulz S, Tikhonova IG, Tobin AB, Milligan G -
Selective xyloglucan oligosaccharide hydrolysis by a GH31 α-xylosidase from Escherichia coli.
Carbohydrate polymers 2022 Carneiro LABC, Fuzo CA, Meleiro LP, Carli S, Barreto MQ, Lourenzoni MR, Buckeridge MS, Ward RJ -
Dictamnine, a novel c-Met inhibitor, suppresses the proliferation of lung cancer cells by downregulating the PI3K/AKT/mTOR and MAPK signaling pathways.
Biochemical pharmacology 2021 Yu J, Zhang L, Peng J, Ward R, Hao P, Wang J, Zhang N, Yang Y, Guo X, Xiang C, An S, Xu TR -
Covalent Immobilization of Chondrostereum purpureum Endopolygalacturonase on Ferromagnetic Nanoparticles: Catalytic Properties and Biotechnological Application.
Applied biochemistry and biotechnology 2021 Carli S, Salgado JCS, Meleiro LP, Ward RJ -
Breast adipose regulation of premenopausal breast epithelial phenotype involves interleukin 10.
Journal of molecular endocrinology 2021 Alhallak I, Wolter KG, Castro Munoz A, Simmen FA, Ward RJ, Petty SA, Li LX, Simmen RCM -
The monocyte-to-lymphocyte ratio: Sex-specific differences in the tuberculosis disease spectrum, diagnostic indices and defining normal ranges.
PloS one 2021 Buttle TS, Hummerstone CY, Billahalli T, Ward RJB, Barnes KE, Marshall NJ, Spong VC, Bothamley GH -
Enhanced hydrolytic efficiency of an engineered CBM11-glucanase enzyme chimera against barley β-d-glucan extracts.
Food chemistry 2021 Furtado GP, Carli S, Meleiro LP, Salgado JCS, Ward RJ -
The C-terminal of the α1b-adreneroceptor is a key determinant for its structure integrity and biological functions.
Bioscience, biotechnology, and biochemistry 2021 Liu Y, Shao YT, Ward R, Ma L, Gui HX, Hao Q, Mu X, Yang Y, An S, Guo XX, Xu TR -
Plant cell wall architecture guided design of CBM3-GH11 chimeras with enhanced xylanase activity using a tandem repeat left-handed β-3-prism scaffold.
Computational and structural biotechnology journal 2021 Pinheiro MP, Reis RAG, Dupree P, Ward RJ -
Chemokine receptor CXCR4 oligomerization is disrupted selectively by the antagonist ligand IT1t.
The Journal of biological chemistry 2020 Ward RJ, Pediani JD, Marsango S, Jolly R, Stoneman MR, Biener G, Handel TM, Raicu V, Milligan G -
Eukaryotic translation initiation factors as promising targets in cancer therapy.
Cell communication and signaling : CCS 2020 Hao P, Yu J, Ward R, Liu Y, Hao Q, An S, Xu T -
A Highly Glucose Tolerant ß-Glucosidase from Malbranchea pulchella (MpBg3) Enables Cellulose Saccharification.
Scientific reports 2020 Monteiro LMO, Vici AC, Pinheiro MP, Heinen PR, de Oliveira AHC, Ward RJ, Prade RA, Buckeridge MS, Polizeli MLTM -
A Halotolerant Endo-1,4-β-Xylanase from Aspergillus clavatus with Potential Application for Agroindustrial Residues Saccharification.
Applied biochemistry and biotechnology 2020 Pasin TM, Salgado JCS, Scarcella ASA, de Oliveira TB, de Lucas RC, Cereia M, Rosa JC, Ward RJ, Buckeridge MS, Polizeli MLTM -
A novel Trichoderma reesei mutant RP698 with enhanced cellulase production.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 2019 Silva JCR, Salgado JCS, Vici AC, Ward RJ, Polizeli MLTM, Guimarães LHS, Furriel RPM, Jorge JA -
Nutrient and drought stress: implications for phenology and biomass quality in miscanthus.
Annals of botany 2019 da Costa RMF, Simister R, Roberts LA, Timms-Taravella E, Cambler AB, Corke FMK, Han J, Ward RJ, Buckeridge MS, Gomez LD, Bosch M -
Biochemical and kinetic characterization of the recombinant GH28 Stereum purpureum endopolygalacturonase and its biotechnological application.
International journal of biological macromolecules 2019 Carli S, Meleiro LP, Ward RJ -
A general method to quantify ligand-driven oligomerization from fluorescence-based images.
Nature methods 2019 Stoneman MR, Biener G, Ward RJ, Pediani JD, Badu D, Eis A, Popa I, Milligan G, Raicu V -
Immobilization of a β-glucosidase and an endoglucanase in ferromagnetic nanoparticles: A study of synergistic effects.
Protein expression and purification 2019 Carli S, Carneiro LABC, Ward RJ, Meleiro LP -
Design, Synthesis, and Evaluation of a Diazirine Photoaffinity Probe for Ligand-Based Receptor Capture Targeting G Protein-Coupled Receptors.
Molecular pharmacology 2018 Müskens FM, Ward RJ, Herkt D, van de Langemheen H, Tobin AB, Liskamp RMJ, Milligan G -
GPCR homo-oligomerization.
Current opinion in cell biology 2018 Milligan G, Ward RJ, Marsango S -
Engineering the affinity of a family 11 carbohydrate binding module to improve binding of branched over unbranched polysaccharides.
International journal of biological macromolecules 2018 Furtado GP, Lourenzoni MR, Fuzo CA, Fonseca-Maldonado R, Guazzaroni ME, Ribeiro LF, Ward RJ -
Protein Engineering Strategies to Expand CRISPR-Cas9 Applications.
International journal of genomics 2018 Ribeiro LF, Ribeiro LFC, Barreto MQ, Ward RJ -
Glucose tolerant and glucose stimulated β-glucosidases - A review.
Bioresource technology 2018 Salgado JCS, Meleiro LP, Carli S, Ward RJ -
Characterization of a β-galactosidase from Bacillus subtilis with transgalactosylation activity.
International journal of biological macromolecules 2018 Carneiro LABC, Yu L, Dupree P, Ward RJ -
Visualization of the activation of the histamine H3 receptor (H3R) using novel fluorescence resonance energy transfer biosensors and their potential application to the study of H3R pharmacology.
The FEBS journal 2018 Liu Y, Zeng H, Pediani JD, Ward RJ, Chen LY, Wu N, Ma L, Tang M, Yang Y, An S, Guo XX, Hao Q, Xu TR -
Assessing the real-time activation of the cannabinoid CB1 receptor and the associated structural changes using a FRET biosensor.
The international journal of biochemistry & cell biology 2018 Liu Y, Chen LY, Zeng H, Ward R, Wu N, Ma L, Mu X, Li QL, Yang Y, An S, Guo XX, Hao Q, Xu TR -
Functionalization of paramagnetic nanoparticles for protein immobilization and purification.
Analytical biochemistry 2017 Carneiro LABC, Ward RJ -
Overexpression of a Cellobiose-Glucose-Halotolerant Endoglucanase from Scytalidium thermophilum.
Applied biochemistry and biotechnology 2017 Meleiro LP, Carli S, Fonseca-Maldonado R, da Silva Torricillas M, Zimbardi ALRL, Ward RJ, Jorge JA, Furriel RPM -
Engineering the GH1 β-glucosidase from Humicola insolens: Insights on the stimulation of activity by glucose and xylose.
PloS one 2017 Meleiro LP, Salgado JCS, Maldonado RF, Carli S, Moraes LAB, Ward RJ, Jorge JA, Furriel RPM -
Lignocellulose binding of a Cel5A-RtCBM11 chimera with enhanced β-glucanase activity monitored by electron paramagnetic resonance.
Biotechnology for biofuels 2017 Fonseca-Maldonado R, Meleiro LP, Mendes LFS, Alves LF, Carli S, Morero LD, Basso LGM, Costa-Filho AJ, Ward RJ -
Muscarinic receptor oligomerization.
Neuropharmacology 2017 Marsango S, Ward RJ, Alvarez-Curto E, Milligan G -
Spatial Intensity Distribution Analysis: Studies of G Protein-Coupled Receptor Oligomerisation.
Trends in pharmacological sciences 2017 Pediani JD, Ward RJ, Marsango S, Milligan G -
Spatial intensity distribution analysis quantifies the extent and regulation of homodimerization of the secretin receptor.
The Biochemical journal 2017 Ward RJ, Pediani JD, Harikumar KG, Miller LJ, Milligan G -
A Molecular Basis for Selective Antagonist Destabilization of Dopamine D3 Receptor Quaternary Organization.
Scientific reports 2017 Marsango S, Caltabiano G, Jiménez-Rosés M, Millan MJ, Pediani JD, Ward RJ, Milligan G -
Methods used to study the oligomeric structure of G-protein-coupled receptors.
Bioscience reports 2017 Guo H, An S, Ward R, Yang Y, Liu Y, Guo XX, Hao Q, Xu TR -
Structural determinants of the hyperalgesic activity of myotoxic Lys49-phospholipase A2.
The journal of venomous animals and toxins including tropical diseases 2017 Zambelli VO, Chioato L, Gutierrez VP, Ward RJ, Cury Y -
The role of local residue environmental changes in thermostable mutants of the GH11 xylanase from Bacillus subtilis.
International journal of biological macromolecules 2017 Silva SB, Pinheiro MP, Fuzo CA, Silva SR, Ferreira TL, Lourenzoni MR, Nonato MC, Vieira DS, Ward RJ -
A xylose-stimulated xylanase-xylose binding protein chimera created by random nonhomologous recombination.
Biotechnology for biofuels 2016 Ribeiro LF, Tullman J, Nicholes N, Silva SR, Vieira DS, Ostermeier M, Ward RJ -
Dynamic Regulation of Quaternary Organization of the M1 Muscarinic Receptor by Subtype-selective Antagonist Drugs.
The Journal of biological chemistry 2016 Pediani JD, Ward RJ, Godin AG, Marsango S, Milligan G -
G protein-coupled receptors as promising cancer targets.
Cancer letters 2016 Liu Y, An S, Ward R, Yang Y, Guo XX, Li W, Xu TR -
Thermostabilization of Bacillus subtilis GH11 xylanase by surface charge engineering.
International journal of biological macromolecules 2016 Alponti JS, Fonseca Maldonado R, Ward RJ -
GH53 Endo-Beta-1,4-Galactanase from a Newly Isolated Bacillus licheniformis CBMAI 1609 as an Enzymatic Cocktail Supplement for Biomass Saccharification.
Applied biochemistry and biotechnology 2016 de Lima EA, Machado CB, Zanphorlin LM, Ward RJ, Sato HH, Ruller R -
Social immunity of the family: parental contributions to a public good modulated by brood size.
Evolutionary ecology 2015 Duarte A, Cotter SC, Reavey CE, Ward RJ, De Gasperin O, Kilner RM -
A-Raf: A new star of the family of raf kinases.
Critical reviews in biochemistry and molecular biology 2015 An S, Yang Y, Ward R, Liu Y, Guo XX, Xu TR -
Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis.
Frontiers in microbiology 2015 Vici AC, da Cruz AF, Facchini FD, de Carvalho CC, Pereira MG, Fonseca-Maldonado R, Ward RJ, Pessela BC, Fernandez-Lorente G, Torres FA, Jorge JA, Polizeli ML -
Biased signalling: the instinctive skill of the cell in the selection of appropriate signalling pathways.
The Biochemical journal 2015 Liu Y, Yang Y, Ward R, An S, Guo XX, Li W, Xu TR -
Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase.
Biotechnology for biofuels 2015 Ribeiro LF, Nicholes N, Tullman J, Ribeiro LF, Fuzo CA, Vieira DS, Furtado GP, Ostermeier M, Ward RJ -
Regulation of oligomeric organization of the serotonin 5-hydroxytryptamine 2C (5-HT2C) receptor observed by spatial intensity distribution analysis.
The Journal of biological chemistry 2015 Ward RJ, Pediani JD, Godin AG, Milligan G -
Increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from Bacillus licheniformis.
Biotechnology letters 2015 Machado CB, Citadini AP, Goldbeck R, de Lima EA, Figueiredo FL, da Silva TM, Hoffmam ZB, de Sousa AS, Squina FM, de Lourdes Teixeira de Moraes Polizeli M, Ruller R, Ward RJ -
The molecular basis of oligomeric organization of the human M3 muscarinic acetylcholine receptor.
Molecular pharmacology 2015 Liste MJ, Caltabiano G, Ward RJ, Alvarez-Curto E, Marsango S, Milligan G -
Enhanced xyloglucan-specific endo-β-1,4-glucanase efficiency in an engineered CBM44-XegA chimera.
Applied microbiology and biotechnology 2015 Furtado GP, Santos CR, Cordeiro RL, Ribeiro LF, de Moraes LA, Damásio AR, Polizeli Mde L, Lourenzoni MR, Murakami MT, Ward RJ -
Raf-interactome in tuning the complexity and diversity of Raf function.
The FEBS journal 2014 An S, Yang Y, Ward R, Liu Y, Guo XX, Xu TR -
Characterization of VPS34-IN1, a selective inhibitor of Vps34, reveals that the phosphatidylinositol 3-phosphate-binding SGK3 protein kinase is a downstream target of class III phosphoinositide 3-kinase.
The Biochemical journal 2014 Bago R, Malik N, Munson MJ, Prescott AR, Davies P, Sommer E, Shpiro N, Ward R, Cross D, Ganley IG, Alessi DR -
Synthetic biology approaches to improve biocatalyst identification in metagenomic library screening.
Microbial biotechnology 2014 Guazzaroni ME, Silva-Rocha R, Ward RJ -
Concommitant adaptation of a GH11 xylanase by directed evolution to create an alkali-tolerant/thermophilic enzyme.
Protein engineering, design & selection : PEDS 2014 Ruller R, Alponti J, Deliberto LA, Zanphorlin LM, Machado CB, Ward RJ -
A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology.
Biotechnology for biofuels 2014 Ribeiro LF, De Lucas RC, Vitcosque GL, Ribeiro LF, Ward RJ, Rubio MV, Damásio AR, Squina FM, Gregory RC, Walton PH, Jorge JA, Prade RA, Buckeridge MS, Polizeli Mde L -
Roundabout 1 exists predominantly as a basal dimeric complex and this is unaffected by binding of the ligand Slit2.
The Biochemical journal 2014 Zakrys L, Ward RJ, Pediani JD, Godin AG, Graham GJ, Milligan G -
Structural basis for glucose tolerance in GH1 β-glucosidases.
Acta crystallographica. Section D, Biological crystallography 2014 de Giuseppe PO, Souza Tde A, Souza FH, Zanphorlin LM, Machado CB, Ward RJ, Jorge JA, Furriel Rdos P, Murakami MT -
The histone chaperones Vps75 and Nap1 form ring-like, tetrameric structures in solution.
Nucleic acids research 2014 Bowman A, Hammond CM, Stirling A, Ward R, Shang W, El-Mkami H, Robinson DA, Svergun DI, Norman DG, Owen-Hughes T -
Biochemical properties of glycosylation and characterization of a histidine acid phosphatase (phytase) expressed in Pichia pastoris.
Protein expression and purification 2014 Fonseca-Maldonado R, Maller A, Bonneil E, Thibault P, Botelho-Machado C, Ward RJ, Polizeli Mde L -
Characterization of a periplasmic nitrate reductase in complex with its biosynthetic chaperone.
The FEBS journal 2013 Dow JM, Grahl S, Ward R, Evans R, Byron O, Norman DG, Palmer T, Sargent F -
D-xylose detection in Escherichia coli by a xylose binding protein-dependent response.
Journal of biotechnology 2013 Ribeiro LF, Bressan F, Furtado GP, Meireles F, Ward RJ -
Novel oxytocin receptor agonists and antagonists: a patent review (2002 - 2013).
Expert opinion on therapeutic patents 2013 Yang Y, Li H, Ward R, Gao L, Wei JF, Xu TR -
Orexin receptors: multi-functional therapeutic targets for sleeping disorders, eating disorders, drug addiction, cancers and other physiological disorders.
Cellular signalling 2013 Xu TR, Yang Y, Ward R, Gao L, Liu Y -
Engineering the pattern of protein glycosylation modulates the thermostability of a GH11 xylanase.
The Journal of biological chemistry 2013 Fonseca-Maldonado R, Vieira DS, Alponti JS, Bonneil E, Thibault P, Ward RJ -
Defining the molecular basis for the first potent and selective orthosteric agonists of the FFA2 free fatty acid receptor.
The Journal of biological chemistry 2013 Hudson BD, Due-Hansen ME, Christiansen E, Hansen AM, Mackenzie AE, Murdoch H, Pandey SK, Ward RJ, Marquez R, Tikhonova IG, Ulven T, Milligan G -
Structural and biophysical characterisation of G protein-coupled receptor ligand binding using resonance energy transfer and fluorescent labelling techniques.
Biochimica et biophysica acta 2013 Ward RJ, Milligan G -
GPCR oligomerization and receptor trafficking.
Methods in enzymology 2013 Ward RJ, Xu TR, Milligan G -
Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double resonance (PELDOR) spectroscopy.
Proceedings of the National Academy of Sciences of the United States of America 2012 Pliotas C, Ward R, Branigan E, Rasmussen A, Hagelueken G, Huang H, Black SS, Booth IR, Schiemann O, Naismith JH -
Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis.
The EMBO journal 2012 Laarman AJ, Mijnheer G, Mootz JM, van Rooijen WJ, Ruyken M, Malone CL, Heezius EC, Ward R, Milligan G, van Strijp JA, de Haas CJ, Horswill AR, van Kessel KP, Rooijakkers SH -
The bactericidal effect of human secreted group IID phospholipase A2 results from both hydrolytic and non-hydrolytic activities.
Biochimie 2012 Fonseca-Maldonado R, Ferreira TL, Ward RJ -
Intramolecular fluorescence resonance energy transfer (FRET) sensors of the orexin OX1 and OX2 receptors identify slow kinetics of agonist activation.
The Journal of biological chemistry 2012 Xu TR, Ward RJ, Pediani JD, Milligan G -
Characterization of suramin binding sites on the human group IIA secreted phospholipase A2 by site-directed mutagenesis and molecular dynamics simulation.
Archives of biochemistry and biophysics 2012 Aragão EA, Vieira DS, Chioato L, Ferreira TL, Lourenzoni MR, Silva SR, Ward RJ -
Dissecting structure-function-stability relationships of a thermostable GH5-CBM3 cellulase from Bacillus subtilis 168.
The Biochemical journal 2012 Santos CR, Paiva JH, Sforça ML, Neves JL, Navarro RZ, Cota J, Akao PK, Hoffmam ZB, Meza AN, Smetana JH, Nogueira ML, Polikarpov I, Xavier-Neto J, Squina FM, Ward RJ, Ruller R, Zeri AC, Murakami MT -
Functional characterization and oligomerization of a recombinant xyloglucan-specific endo-β-1,4-glucanase (GH12) from Aspergillus niveus.
Biochimica et biophysica acta 2011 Damásio AR, Ribeiro LF, Ribeiro LF, Furtado GP, Segato F, Almeida FB, Crivellari AC, Buckeridge MS, Souza TA, Murakami MT, Ward RJ, Prade RA, Polizeli ML -
Analysis of conformational changes in the DNA junction-resolving enzyme T7 endonuclease I on binding a four-way junction using EPR.
Biochemistry 2011 Freeman AD, Ward R, El Mkami H, Lilley DM, Norman DG -
Engineering bifunctional laccase-xylanase chimeras for improved catalytic performance.
The Journal of biological chemistry 2011 Ribeiro LF, Furtado GP, Lourenzoni MR, Costa-Filho AJ, Santos CR, Nogueira SC, Betini JA, Polizeli Mde L, Murakami MT, Ward RJ -
Active site mutants of human secreted Group IIA Phospholipase A2 lacking hydrolytic activity retain their bactericidal effect.
Biochimie 2011 Chioato L, Aragão EA, Ferreira TL, Ward RJ -
The orexin OX(1) receptor exists predominantly as a homodimer in the basal state: potential regulation of receptor organization by both agonist and antagonist ligands.
The Biochemical journal 2011 Xu TR, Ward RJ, Pediani JD, Milligan G -
Heteromultimerization of cannabinoid CB(1) receptor and orexin OX(1) receptor generates a unique complex in which both protomers are regulated by orexin A.
The Journal of biological chemistry 2011 Ward RJ, Pediani JD, Milligan G -
Conformation analysis of a surface loop that controls active site access in the GH11 xylanase A from Bacillus subtilis.
Journal of molecular modeling 2011 Vieira DS, Ward RJ -
Molecular adaptability of nucleoside diphosphate kinase b from trypanosomatid parasites: stability, oligomerization and structural determinants of nucleotide binding.
Molecular bioSystems 2011 Souza TA, Trindade DM, Tonoli CC, Santos CR, Ward RJ, Arni RK, Oliveira AH, Murakami MT -
Extracellular loop 2 of the free fatty acid receptor 2 mediates allosterism of a phenylacetamide ago-allosteric modulator.
Molecular pharmacology 2011 Smith NJ, Ward RJ, Stoddart LA, Hudson BD, Kostenis E, Ulven T, Morris JC, Tränkle C, Tikhonova IG, Adams DR, Milligan G -
Ligand-induced internalization of the orexin OX(1) and cannabinoid CB(1) receptors assessed via N-terminal SNAP and CLIP-tagging.
British journal of pharmacology 2011 Ward RJ, Pediani JD, Milligan G -
The histone chaperones Nap1 and Vps75 bind histones H3 and H4 in a tetrameric conformation.
Molecular cell 2011 Bowman A, Ward R, Wiechens N, Singh V, El-Mkami H, Norman DG, Owen-Hughes T -
Selective orthosteric free fatty acid receptor 2 (FFA2) agonists: identification of the structural and chemical requirements for selective activation of FFA2 versus FFA3.
The Journal of biological chemistry 2011 Schmidt J, Smith NJ, Christiansen E, Tikhonova IG, Grundmann M, Hudson BD, Ward RJ, Drewke C, Milligan G, Kostenis E, Ulven T -
A practical teaching course in directed protein evolution using the green fluorescent protein as a model.
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology 2011 Ruller R, Silva-Rocha R, Silva A, Cruz Schneider MP, Ward RJ -
Using the Flp-In™ T-Rex™ system to regulate GPCR expression.
Methods in molecular biology (Clifton, N.J.) 2011 Ward RJ, Alvarez-Curto E, Milligan G -
Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogeneous time-resolved FRET.
The Journal of biological chemistry 2010 Alvarez-Curto E, Ward RJ, Pediani JD, Milligan G -
A nurse-led response to unmet needs of homeless migrants in inner London.
British journal of nursing (Mark Allen Publishing) 2010 Collinson S, Ward R -
Probing the (H3-H4)2 histone tetramer structure using pulsed EPR spectroscopy combined with site-directed spin labelling.
Nucleic acids research 2009 Bowman A, Ward R, El-Mkami H, Owen-Hughes T, Norman DG -
Purification and biochemical characterization of a novel alpha-glucosidase from Aspergillus niveus.
Antonie van Leeuwenhoek 2009 da Silva TM, Michelin M, Damásio AR, Maller A, Almeida FB, Ruller R, Ward RJ, Rosa JC, Jorge JA, Terenzi HF, Polizeli Mde L -
Production, purification, crystallization and preliminary X-ray diffraction studies of the nucleoside diphosphate kinase b from Leishmania major.
Acta crystallographica. Section F, Structural biology and crystallization communications 2009 Tonoli CC, Vieira PS, Ward RJ, Arni RK, de Oliveira AH, Murakami MT -
Shared structural determinants for the calcium-independent liposome membrane permeabilization and sarcolemma depolarization in Bothropstoxin-I, a LYS49-PLA(2) from the venom of Bothrops jararacussu.
The international journal of biochemistry & cell biology 2009 Aragão EA, Randazzo-Moura P, Rostelato-Ferreira S, Rodrigues-Simioni L, Ward RJ -
Properties of a purified thermostable glucoamylase from Aspergillus niveus.
Journal of industrial microbiology & biotechnology 2009 da Silva TM, Maller A, Damásio AR, Michelin M, Ward RJ, Hirata IY, Jorge JA, Terenzi HF, de Polizeli ML -
The interaction of bothropstoxin-I (Lys49-PLA(2)) with liposome membranes.
Toxicon : official journal of the International Society on Toxinology 2009 Ferreira TL, Ward RJ -
Reduced pH induces an inactive non-native conformation of the monomeric bothropstoxin-I (Lys49-PLA2).
Toxicon : official journal of the International Society on Toxinology 2009 de Oliveira AH, Ferreira TL, Ward RJ -
Characterization of temperature dependent and substrate-binding cleft movements in Bacillus circulans family 11 xylanase: a molecular dynamics investigation.
Biochimica et biophysica acta 2009 Vieira DS, Degrève L, Ward RJ -
Refolding and purification of the human secreted group IID phospholipase A2 expressed as inclusion bodies in Escherichia coli.
Protein expression and purification 2009 Fonseca RG, Ferreira TL, Ward RJ -
Mapping of suramin binding sites on the group IIA human secreted phospholipase A2.
Bioorganic chemistry 2009 Vieira DS, Aragão EA, Lourenzoni MR, Ward RJ -
Selectivity and functional consequences of interactions of family A G protein-coupled receptors with neurochondrin and periplakin.
Journal of neurochemistry 2009 Ward RJ, Jenkins L, Milligan G -
The association of Na,K-ATPase subunits studied by circular dichroism, surface tension and dilatational elasticity.
Journal of colloid and interface science 2008 Rigos CF, Nobre TM, Zaniquelli ME, Ward RJ, Ciancaglini P -
Thermostable variants of the recombinant xylanase A from Bacillus subtilis produced by directed evolution show reduced heat capacity changes.
Proteins 2008 Ruller R, Deliberto L, Ferreira TL, Ward RJ -
Parents influence asymmetric sibling competition: experimental evidence with partially dependent young.
Ecology 2007 Smiseth PT, Ward RJ, Moore AJ -
Permeabilization of E. coli K12 inner and outer membranes by bothropstoxin-I, A LYS49 phospholipase A2 from Bothrops jararacussu.
Toxicon : official journal of the International Society on Toxinology 2007 Aragão EA, Chioato L, Ward RJ -
Insights on calcium-independent phospholipid membrane damage by Lys49-PLA2 using tryptophan scanning mutagenesis of bothropstoxin-I from Bothrops jararacussu.
Biochimie 2007 Ferreira TL, Ruller R, Chioato L, Ward RJ -
Purification and biochemical characterization of a thermostable extracellular glucoamylase produced by the thermotolerant fungus Paecilomyces variotii.
Journal of industrial microbiology & biotechnology 2007 Michelin M, Ruller R, Ward RJ, Moraes LA, Jorge JA, Terenzi HF, Polizeli Mde L -
Mapping of the structural determinants of artificial and biological membrane damaging activities of a Lys49 phospholipase A2 by scanning alanine mutagenesis.
Biochimica et biophysica acta 2007 Chioato L, Aragão EA, Lopes Ferreira T, Medeiros AI, Faccioli LH, Ward RJ -
An ultraviolet photoacoustic spectroscopy study of the interaction between Lys49-phospholipase A2 and amphiphilic molecules.
Biochemical and biophysical research communications 2006 Bugs MR, Bortoleto-Bugs RK, Cornélio ML, Ward RJ -
Interaction of neurochondrin with the melanin-concentrating hormone receptor 1 interferes with G protein-coupled signal transduction but not agonist-mediated internalization.
The Journal of biological chemistry 2006 Francke F, Ward RJ, Jenkins L, Kellett E, Richter D, Milligan G, Bächner D -
Molecular modeling of the human eukaryotic translation initiation factor 5A (eIF5A) based on spectroscopic and computational analyses.
Biochemical and biophysical research communications 2006 Costa-Neto CM, Parreiras-E-Silva LT, Ruller R, Oliveira EB, Miranda A, Oliveira L, Ward RJ -
Subproteomic analysis of soluble proteins of the microsomal fraction from two Leishmania species.
Comparative biochemistry and physiology. Part D, Genomics & proteomics 2006 de Oliveira AH, Ruiz JC, Cruz AK, Greene LJ, Rosa JC, Ward RJ -
Expression in E. coli and purification of the nucleoside diphosphate kinase b from Leishmania major.
Protein expression and purification 2006 de Oliveira AH, Ruiz JC, Cruz AK, Greene LJ, Rosa JC, Ward RJ -
Influence of aqueous aluminium on the immune system of the freshwater crayfish Pacifasticus leniusculus.
Aquatic toxicology (Amsterdam, Netherlands) 2006 Ward RJ, McCrohan CR, White KN -
Efficient constitutive expression of Bacillus subtilis xylanase A in Escherichia coli DH5alpha under the control of the Bacillus BsXA promoter.
Biotechnology and applied biochemistry 2006 Ruller R, Rosa JC, Faça VM, Greene LJ, Ward RJ -
Lipid bilayer stabilization of the Na,K-ATPase reconstituted in DPPC/DPPE liposomes.
Cell biochemistry and biophysics 2006 Rigos CF, de Lima Santos H, Ward RJ, Ciancaglini P -
Purification and characterization of two xylanases from alkalophilic and thermophilic Bacillus licheniformis 77-2.
Applied biochemistry and biotechnology 2006 Damiano VB, Ward R, Gomes E, Alves-Prado HF, Da Silva R -
Functional interactions between the alpha1b-adrenoceptor and Galpha11 are compromised by de-palmitoylation of the G protein but not of the receptor.
Cellular signalling 2005 Novotny J, Durchankova D, Ward RJ, Carrillo JJ, Svoboda P, Milligan G -
Correlation of temperature induced conformation change with optimum catalytic activity in the recombinant G/11 xylanase A from Bacillus subtilis strain 168 (1A1).
FEBS letters 2005 Murakami MT, Arni RK, Vieira DS, Degrève L, Ruller R, Ward RJ -
A key serine for the GTPase-activating protein function of regulator of G protein signaling proteins is not a general target for 14-3-3 interactions.
Molecular pharmacology 2005 Ward RJ, Milligan G -
Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: potentiation of hemolytic activity by LipL32.
Biochemical and biophysical research communications 2005 Hauk P, Negrotto S, Romero EC, Vasconcellos SA, Genovez ME, Ward RJ, Schattner M, Goméz RM, Ho PL -
Structural insights for fatty acid binding in a Lys49-phospholipase A2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid.
Biochimie 2005 Watanabe L, Soares AM, Ward RJ, Fontes MR, Arni RK -
A molecular mechanism for Lys49-phospholipase A2 activity based on ligand-induced conformational change.
The Journal of biological chemistry 2004 Ambrosio AL, Nonato MC, de Araújo HS, Arni R, Ward RJ, Ownby CL, de Souza DH, Garratt RC -
Ontogenetic changes in Phoneutria nigriventer (Araneae, Ctenidae) spider venom.
Toxicon : official journal of the International Society on Toxinology 2004 Herzig V, Ward RJ, Dos Santos WF -
Activation of Ca2+-independent membrane-damaging activity in Lys49-phospholipase A2 promoted by amphiphilic molecules.
Biochemical and biophysical research communications 2004 Bortoleto-Bugs RK, Neto AA, Ward RJ -
Topology of the substrate-binding site of a Lys49-phospholipase A2 influences Ca2+-independent membrane-damaging activity.
The Biochemical journal 2004 Sá JM, Chioato L, Ferreira TL, De Oliveira AH, Ruller R, Rosa JC, Greene LJ, Ward RJ -
Identification of a novel site within G protein alpha subunits important for specificity of receptor-G protein interaction.
Molecular pharmacology 2004 Heydorn A, Ward RJ, Jorgensen R, Rosenkilde MM, Frimurer TM, Milligan G, Kostenis E -
Phe120 contributes to the regiospecificity of cytochrome P450 2D6: mutation leads to the formation of a novel dextromethorphan metabolite.
The Biochemical journal 2004 Flanagan JU, Maréchal JD, Ward R, Kemp CA, McLaughlin LA, Sutcliffe MJ, Roberts GC, Paine MJ, Wolf CR -
The biological activity in mammals and insects of the nucleosidic fraction from the spider Parawixia bistriata.
Toxicon : official journal of the International Society on Toxinology 2004 Rodrigues MC, Guizzo R, Gobbo-Neto L, Ward RJ, Lopes NP, dos Santos WF -
Analysis of function of receptor-G-protein and receptor-RGS fusion proteins.
Methods in molecular biology (Clifton, N.J.) 2004 Ward RJ, Milligan G -
Mapping structural determinants of biological activities in snake venom phospholipases A2 by sequence analysis and site directed mutagenesis.
Toxicon : official journal of the International Society on Toxinology 2003 Chioato L, Ward RJ -
Spectroscopic characterization and structural modeling of prolamin from maize and pearl millet.
European biophysics journal : EBJ 2003 Bugs MR, Forato LA, Bortoleto-Bugs RK, Fischer H, Mascarenhas YP, Ward RJ, Colnago LA -
Concerted stimulation and deactivation of pertussis toxin-sensitive G proteins by chimeric G protein-coupled receptor-regulator of G protein signaling 4 fusion proteins: analysis of the contribution of palmitoylated cysteine residues to the GAP activity of RGS4.
Journal of neurochemistry 2003 Bahia DS, Sartania N, Ward RJ, Cavalli A, Jones TL, Druey KM, Milligan G -
Mechanism of action of Gq to inhibit G beta gamma modulation of CaV2.2 calcium channels: probed by the use of receptor-G alpha tandems.
Molecular pharmacology 2003 Bertaso F, Ward RJ, Viard P, Milligan G, Dolphin AC -
Palmitoylation regulates regulator of G-protein signaling (RGS) 16 function. II. Palmitoylation of a cysteine residue in the RGS box is critical for RGS16 GTPase accelerating activity and regulation of Gi-coupled signalling.
The Journal of biological chemistry 2003 Osterhout JL, Waheed AA, Hiol A, Ward RJ, Davey PC, Nini L, Wang J, Milligan G, Jones TL, Druey KM -
Structural and functional analysis of BmjMIP, a phospholipase A2 myotoxin inhibitor protein from Bothrops moojeni snake plasma.
Biochemical and biophysical research communications 2003 Soares AM, Marcussi S, Stábeli RG, França SC, Giglio JR, Ward RJ, Arantes EC -
Chemical denaturation of a homodimeric lysine-49 phospholipase A2: a stable dimer interface and a native monomeric intermediate.
Archives of biochemistry and biophysics 2003 Ruller R, Ferreira TL, de Oliveira AH, Ward RJ -
Isolation, purification, and physicochemical characterization of a D-galactose-binding lectin from seeds of Erythrina speciosa.
Archives of biochemistry and biophysics 2003 Konozy EH, Bernardes ES, Rosa C, Faca V, Greene LJ, Ward RJ -
Purification, some properties of a D-galactose-binding leaf lectin from Erythrina indica and further characterization of seed lectin.
Biochimie 2002 Konozy EH, Mulay R, Faca V, Ward RJ, Greene LJ, Roque-Barriera MC, Sabharwal S, Bhide SV -
Distinct sites for myotoxic and membrane-damaging activities in the C-terminal region of a Lys49-phospholipase A2.
The Biochemical journal 2002 Chioato L, De Oliveira AH, Ruller R, Sá JM, Ward RJ -
Reciprocal mutations of highly conserved residues in transmembrane helices 2 and 7 of the alpha(2A)-adrenoceptor restore agonist activation of G(i1)alpha.
Cellular signalling 2002 Ward RJ, Milligan G -
Intrathecal methadone: a dose-response study and comparison with intrathecal morphine 0.5 mg.
Pain 1990 Jacobson L, Chabal C, Brody MC, Ward RJ, Wasse L