Petr Kasparek, PhD
Research Associate
Orofacial Sciences
School of Dentistry

Interests

Show all (3) Hide

• synthetic biology
• molecular biology
• genome engineering

Publications (51)

Top publication keywords:
KallikreinsSerpinsCRISPR-Cas SystemsADAM10 ProteinNetherton SyndromeZygoteAngelman SyndromeGene SilencingADAM17 ProteinMosaicismSerine Peptidase Inhibitor Kazal-Type 5HematopoiesisMiceEndonucleasesGene Targeting

• Intrinsically disordered proteins drive enamel formation via an evolutionarily conserved self-assembly motif.
  Proceedings of the National Academy of Sciences of the United States of America 2017 Wald T, Spoutil F, Osickova A, Prochazkova M, Benada O, Kasparek P, Bumba L, Klein OD, Sedlacek R, Sebo P, Prochazka J, Osicka R
• KLK5 and KLK7 Ablation Fully Rescues Lethality of Netherton Syndrome-Like Phenotype.
  PLoS genetics 2017 Kasparek P, Ileninova Z, Zbodakova O, Kanchev I, Benada O, Chalupsky K, Brattsand M, Beck IM, Sedlacek R
• A viable mouse model for Netherton syndrome based on mosaic inactivation of the Spink5 gene.
  Biological chemistry 2016 Kasparek P, Ileninova Z, Haneckova R, Kanchev I, Jenickova I, Sedlacek R
• Efficient gene targeting of the Rosa26 locus in mouse zygotes using TALE nucleases.
  FEBS letters 2014 Kasparek P, Krausova M, Haneckova R, Kriz V, Zbodakova O, Korinek V, Sedlacek R
• Open or closed? Structural characterization of plasma-derived and recombinant human plasminogen across multiple resolution scales.
  International journal of biological macromolecules 2026 Kasiarova L, Verma N, Pinkas M, Henek T, Klumpler T, Chovancikova M, Reutova H, Kasparek P, Hlozankova M, Damborsky J, Novacek J, Hernychova L, Marek M

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• Claudin 1-mediated positioning of DC1 to mTECs is essential for maintenance of central tolerance.
  The Journal of experimental medicine 2026 Brezina J, Brabec T, Machac D, Voboril M, Ballek O, Paces J, Sýkora V, Jancovicová K, Valter E, Kovácová K, Manning J, Tahtahová V, Cepková A, Dobešová M, Dobeš J, Kubovciak J, Kolár M, Kašpárek P, …
• Germline Jak2-R1063H mutation interferes with normal hematopoietic development and increases risk of thrombosis and leukemic transformation.
  Leukemia 2025 Zimolova V, Burocziova M, Berkova L, Grusanovic S, Gursky J, Janotka L, Kasparek P, Pecinova A, Kundrat D, Hrckulak D, Onhajzer J, Jeziskova I, Nekvindova L, Weinbergerova B, Pospisilova S, Doubek M, …
• Increased thermal stability of FGF10 leads to ectopic signaling during development.
  Cellular and molecular life sciences : CMLS 2025 Czyrek AA, Baran K, Hruba E, Horackova A, Bosakova V, Chudzian J, Fafilek B, Laskova V, Stepankova V, Bednar D, Karl K, Kasparek P, Bosakova M, Killinger M, Szotkowska T, Prochazka J, Zieba JT, Rico-…
• Characterisation of SLC38A8 and Its Role in Retinal Pathways and Disease.
  Clinical & experimental ophthalmology 2025 Weiner C, Hecht I, Lindovsky J, Palkova M, Krupkova M, Kasparek P, Prochazka J, Sedlacek R, Kotlyar A, Raini N, Zehavi Y, Yegorov Y, Hilman P, Basel R, Abu-Hamed R, Shomron N, Pras E
• Understanding PACS2 syndrome's pathomechanism by studying E209K and E211K mutations.
  Mammalian genome : official journal of the International Mammalian Genome Society 2024 Zbikowski A, Kowalczyk T, Kasparek P, Prohazka J, Sedlacek R, Ciborowski M, Cysewski D, Lukasiewicz K
• Synthetic organizer cells guide development via spatial and biochemical instructions.
  Cell 2024 Yamada T, Trentesaux C, Brunger JM, Xiao Y, Stevens AJ, Martyn I, Kasparek P, Shroff NP, Aguilar A, Bruneau BG, Boffelli D, Klein OD, Lim WA
• TBK1-associated adapters TANK and AZI2 protect mice against TNF-induced cell death and severe autoinflammatory diseases.
  Nature communications 2024 Ujevic A, Knizkova D, Synackova A, Pribikova M, Trivic T, Dalinskaya A, Drobek A, Niederlova V, Paprckova D, De Guia R, Kasparek P, Prochazka J, Labaj J, Fedosieieva O, Roeck BF, Mihola O, Trachtulec …
• Impact of essential genes on the success of genome editing experiments generating 3313 new genetically engineered mouse lines.
  Scientific reports 2024 Elrick H, Peterson KA, Willis BJ, Lanza DG, Acar EF, Ryder EJ, Teboul L, Kasparek P, Birling MC, Adams DJ, Bradley A, Braun RE, Brown SD, Caulder A, Codner GF, DeMayo FJ, Dickinson ME, Doe B, Duddy G,…
• DDI2 protease controls embryonic development and inflammation via TCF11/NRF1.
  iScience 2024 Nedomova M, Haberecht-Müller S, Möller S, Venz S, Prochazkova M, Prochazka J, Sedlak F, Chawengsaksophak K, Hammer E, Kasparek P, Adamek M, Sedlacek R, Konvalinka J, Krüger E, Grantz Saskova K
• Multistep allelic conversion in mouse pre-implantation embryos by AAV vectors.
  Scientific reports 2024 Nickl P, Jenickova I, Elias J, Kasparek P, Barinka C, Kopkanova J, Sedlacek R
• Characterization of Mice Carrying a Neurodevelopmental Disease-Associated GluN2B(L825V) Variant.
  The Journal of neuroscience : the official journal of the Society for Neuroscience 2024 Candelas Serra M, Kuchtiak V, Kubik-Zahorodna A, Kysilov B, Fili K, Hrcka Krausova B, Abramova V, Dobrovolski M, Harant K, Bozikova P, Cerny J, Prochazka J, Kasparek P, Sedlacek R, Balik A, Smejkalova…
• Ovol2 promoter mutations in mice and human illuminate species-specific phenotypic divergence.
  Human molecular genetics 2024 Sunny SS, Lachova J, Kasparek P, Palkova M, Spoutil F, Prochazka J, Sedlacek R, Liskova P, Kozmik Z
• Epithelial zonation along the mouse and human small intestine defines five discrete metabolic domains.
  Nature cell biology 2024 Zwick RK, Kasparek P, Palikuqi B, Viragova S, Weichselbaum L, McGinnis CS, McKinley KL, Rathnayake A, Vaka D, Nguyen V, Trentesaux C, Reyes E, Gupta AR, Gartner ZJ, Locksley RM, Gardner JM, Itzkovitz …
• Epithelial zonation along the mouse and human small intestine defines five discrete metabolic domains.
  bioRxiv : the preprint server for biology 2023 Zwick RK, Kasparek P, Palikuqi B, Viragova S, Weichselbaum L, McGinnis CS, McKinley KL, Rathnayake A, Vaka D, Nguyen V, Trentesaux C, Reyes E, Gupta AR, Gartner ZJ, Locksley RM, Gardner JM, Itzkovitz …
• Author Correction: A previously uncharacterized Factor Associated with Metabolism and Energy (FAME/C14orf105/CCDC198/1700011H14Rik) is related to evolutionary adaptation, energy balance, and kidney physiology.
  Nature communications 2023 Petersen J, Englmaier L, Artemov AV, Poverennaya I, Mahmoud R, Bouderlique T, Tesarova M, Deviatiiarov R, Szilvásy-Szabó A, Akkuratov EE, Pajuelo Reguera D, Zeberg H, Kaucka M, Kastriti ME, Krivanek J…
• A previously uncharacterized Factor Associated with Metabolism and Energy (FAME/C14orf105/CCDC198/1700011H14Rik) is related to evolutionary adaptation, energy balance, and kidney physiology.
  Nature communications 2023 Petersen J, Englmaier L, Artemov AV, Poverennaya I, Mahmoud R, Bouderlique T, Tesarova M, Deviatiiarov R, Szilvásy-Szabó A, Akkuratov EE, Pajuelo Reguera D, Zeberg H, Kaucka M, Kastriti ME, Krivanek J…
• A structural model of the iRhom-ADAM17 sheddase complex reveals functional insights into its trafficking and activity.
  Cellular and molecular life sciences : CMLS 2023 Kahveci-Türköz S, Bläsius K, Wozniak J, Rinkens C, Seifert A, Kasparek P, Ohm H, Oltzen S, Nieszporek M, Schwarz N, Babendreyer A, Preisinger C, Sedlacek R, Ludwig A, Düsterhöft S
• Early evolution of enamel matrix proteins is reflected by pleiotropy of physiological functions.
  Scientific reports 2023 Spoutil F, Aranaz-Novaliches G, Prochazkova M, Wald T, Novosadova V, Kasparek P, Osicka R, Reseland JE, Lyngstadaas SP, Tiainen H, Bousova K, Vondrasek J, Sedlacek R, Prochazka J
• Ablation of Gabra5 Influences Corticosterone Levels and Anxiety-like Behavior in Mice.
  Genes 2023 Syding LA, Kubik-Zahorodna A, Reguera DP, Nickl P, Hruskova B, Kralikova M, Kopkanova J, Novosadova V, Kasparek P, Prochazka J, Rozman J, Turecek R, Sedlacek R
• Molecular interactions of adaptor protein PSTPIP2 control neutrophil-mediated responses leading to autoinflammation.
  Frontiers in immunology 2022 Pavliuchenko N, Duric I, Kralova J, Fabisik M, Spoutil F, Prochazka J, Kasparek P, Pokorna J, Skopcova T, Sedlacek R, Brdicka T
• Generation and characterization of human U-2 OS cell lines with the CRISPR/Cas9-edited protoporphyrinogen oxidase IX gene.
  Scientific reports 2022 Novakova Z, Milosevic M, Kutil Z, Ondrakova M, Havlinova B, Kasparek P, Sandoval-Acuña C, Korandova Z, Truksa J, Vrbacky M, Rohlena J, Barinka C
• Generation and Characterization of a Novel Angelman Syndrome Mouse Model with a Full Deletion of the Ube3a Gene.
  Cells 2022 Syding LA, Kubik-Zahorodna A, Nickl P, Novosadova V, Kopkanova J, Kasparek P, Prochazka J, Sedlacek R
• FBXO38 Ubiquitin Ligase Controls Sertoli Cell Maturation.
  Frontiers in cell and developmental biology 2022 Dibus N, Zobalova E, Monleon MAM, Korinek V, Filipp D, Petrusova J, Sedlacek R, Kasparek P, Cermak L
• Comprehensive Transcriptional Profiling and Mouse Phenotyping Reveals Dispensable Role for Adipose Tissue Selective Long Noncoding RNA Gm15551.
  Non-coding RNA 2022 Engelhard CA, Huang C, Khani S, Kasparek P, Prochazka J, Rozman J, Reguera DP, Sedlacek R, Kornfeld JW
• Novel ADNP Syndrome Mice Reveal Dramatic Sex-Specific Peripheral Gene Expression With Brain Synaptic and Tau Pathologies.
  Biological psychiatry 2021 Karmon G, Sragovich S, Hacohen-Kleiman G, Ben-Horin-Hazak I, Kasparek P, Schuster B, Sedlacek R, Pasmanik-Chor M, Theotokis P, Touloumi O, Zoidou S, Huang L, Wu PY, Shi R, Kapitansky O, Lobyntseva A, …
• Importing genetically altered animals: ensuring quality.
  Mammalian genome : official journal of the International Mammalian Genome Society 2021 Birling MC, Fray MD, Kasparek P, Kopkanova J, Massimi M, Matteoni R, Montoliu L, Nutter LMJ, Raspa M, Rozman J, Ryder EJ, Scavizzi F, Voikar V, Wells S, Pavlovic G, Teboul L
• ADAM10 and ADAM17 regulate EGFR, c-Met and TNF RI signalling in liver regeneration and fibrosis.
  Scientific reports 2021 Zbodakova O, Chalupsky K, Sarnova L, Kasparek P, Jirouskova M, Gregor M, Sedlacek R
• CRL4-DCAF12 Ubiquitin Ligase Controls MOV10 RNA Helicase during Spermatogenesis and T Cell Activation.
  International journal of molecular sciences 2021 Lidak T, Baloghova N, Korinek V, Sedlacek R, Balounova J, Kasparek P, Cermak L
• The iRhom homology domain is indispensable for ADAM17-mediated TNFα and EGF receptor ligand release.
  Cellular and molecular life sciences : CMLS 2021 Düsterhöft S, Kahveci-Türköz S, Wozniak J, Seifert A, Kasparek P, Ohm H, Liu S, Kopkanova J, Lokau J, Garbers C, Preisinger C, Sedlacek R, Freeman M, Ludwig A
• Response to correspondence on "Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation".
  Genome biology 2021 Gurumurthy CB, O'Brien AR, Quadros RM, Adams J, Alcaide P, Ayabe S, Ballard J, Batra SK, Beauchamp MC, Becker KA, Bernas G, Brough D, Carrillo-Salinas F, Chan W, Chen H, Dawson R, DeMambro V, D'Hont J…
• A resource of targeted mutant mouse lines for 5,061 genes.
  Nature genetics 2021 Birling MC, Yoshiki A, Adams DJ, Ayabe S, Beaudet AL, Bottomley J, Bradley A, Brown SDM, Bürger A, Bushell W, Chiani F, Chin HG, Christou S, Codner GF, DeMayo FJ, Dickinson ME, Doe B, Donahue LR, Fray…
• Bardet-Biedl Syndrome ciliopathy is linked to altered hematopoiesis and dysregulated self-tolerance.
  EMBO reports 2021 Tsyklauri O, Niederlova V, Forsythe E, Prasai A, Drobek A, Kasparek P, Sparks K, Trachtulec Z, Prochazka J, Sedlacek R, Beales P, Huranova M, Stepanek O
• Intronic Determinants Coordinate Charme lncRNA Nuclear Activity through the Interaction with MATR3 and PTBP1.
  Cell reports 2020 Desideri F, Cipriano A, Petrezselyova S, Buonaiuto G, Santini T, Kasparek P, Prochazka J, Janson G, Paiardini A, Calicchio A, Colantoni A, Sedlacek R, Bozzoni I, Ballarino M
• The iRhom2/ADAM17 Axis Attenuates Bacterial Uptake by Phagocytes in a Cell Autonomous Manner.
  International journal of molecular sciences 2020 Seifert A, Wozniak J, Düsterhöft S, Kasparek P, Sedlacek R, Dreschers S, Orlikowsky TW, Yildiz D, Ludwig A
• Efficient allele conversion in mouse zygotes and primary cells based on electroporation of Cre protein.
  Methods (San Diego, Calif.) 2020 Jenickova I, Kasparek P, Petrezselyova S, Elias J, Prochazka J, Kopkanova J, Navratil M, Barinka C, Sedlacek R
• CRISPR/Cas9 Epigenome Editing Potential for Rare Imprinting Diseases: A Review.
  Cells 2020 Syding LA, Nickl P, Kasparek P, Sedlacek R
• CRMP2 mediates Sema3F-dependent axon pruning and dendritic spine remodeling.
  EMBO reports 2020 Ziak J, Weissova R, Jerábková K, Janikova M, Maimon R, Petrasek T, Pukajova B, Kleisnerova M, Wang M, Brill MS, Kasparek P, Zhou X, Alvarez-Bolado G, Sedlacek R, Misgeld T, Stuchlik A, Perlson E, …
• Truncated PPM1D impairs stem cell response to genotoxic stress and promotes growth of APC-deficient tumors in the mouse colon.
  Cell death & disease 2019 Burocziova M, Burdova K, Martinikova AS, Kasparek P, Kleiblova P, Danielsen SA, Borecka M, Jenikova G, Janecková L, Pavel J, Zemankova P, Schneiderova M, Schwarzova L, Ticha I, Sun XF, Jiraskova K, …
• Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation.
  Genome biology 2019 Gurumurthy CB, O'Brien AR, Quadros RM, Adams J, Alcaide P, Ayabe S, Ballard J, Batra SK, Beauchamp MC, Becker KA, Bernas G, Brough D, Carrillo-Salinas F, Chan W, Chen H, Dawson R, DeMambro V, D'Hont J…
• Kallikrein-related peptidase 5 and seasonal influenza viruses, limitations of the experimental models for activating proteases.
  Biological chemistry 2018 Magnen M, Elsässer BM, Zbodakova O, Kasparek P, Gueugnon F, Petit-Courty A, Sedlacek R, Goettig P, Courty Y
• Profiling system for skin kallikrein proteolysis applied in gene-deficient mouse models.
  Biological chemistry 2018 Horn M, Zbodakova O, Kasparek P, Srp J, Haneckova R, Hradilek M, Mares M, Sedlacek R
• In vivo regulation of the A disintegrin and metalloproteinase 10 (ADAM10) by the tetraspanin 15.
  Cellular and molecular life sciences : CMLS 2018 Seipold L, Altmeppen H, Koudelka T, Tholey A, Kasparek P, Sedlacek R, Schweizer M, Bär J, Mikhaylova M, Glatzel M, Saftig P
• Tetraspanin 3: A central endocytic membrane component regulating the expression of ADAM10, presenilin and the amyloid precursor protein.
  Biochimica et biophysica acta. Molecular cell research 2016 Seipold L, Damme M, Prox J, Rabe B, Kasparek P, Sedlacek R, Altmeppen H, Willem M, Boland B, Glatzel M, Saftig P
• MMP-19 deficiency causes aggravation of colitis due to defects in innate immune cell function.
  Mucosal immunology 2015 Brauer R, Tureckova J, Kanchev I, Khoylou M, Skarda J, Prochazka J, Spoutil F, Beck IM, Zbodakova O, Kasparek P, Korinek V, Chalupsky K, Karhu T, Herzig KH, Hajduch M, Gregor M, Sedlacek R
• Transgenic reporter mice with promoter region of murine LRAT specifically marks lens and meiosis spermatocytes.
  Physiological research 2014 Prukova D, Ileninova Z, Antosova B, Kasparek P, Gregor M, Sedlacek R
• Transgenic mouse model expressing tdTomato under involucrin promoter as a tool for analysis of epidermal differentiation and wound healing.
  Transgenic research 2011 Kasparek P, Krenek P, Buryova H, Suchanova S, Beck IM, Sedlacek R