Nico Stuurman, PhD
Associate Professor
Biochemistry and Biophysics
School of Medicine

Websites

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• CALM (calm.ucsf.edu)
• Github (github.com)

Videos

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• Video (youtube.com)
• Video (youtube.com)
• Video (youtube.com)

Publications (68)

Top publication keywords:
MicroscopyDrosophilaFluorescence Resonance Energy TransferCellsDrosophila ProteinsSpindle ApparatusLaminsImage Processing, Computer-AssistedNuclear ProteinsMicroscopy, InterferenceMicroscopy, Fluorescence, MultiphotonIonophoresMicroscopy, FluorescenceCell BiologyFluorescent Dyes

• Nucleolar dynamics are determined by the ordered assembly of the ribosome.
  The Journal of cell biology 2026 Sheu-Gruttadauria J, Yan X, Stuurman N, Ogrean RO, Lin S, Floor SN, Vale RD
• Nucleolar dynamics are determined by the ordered assembly of the ribosome.
  bioRxiv : the preprint server for biology 2024 Sheu-Gruttadauria J, Yan X, Stuurman N, Vale RD, Floor SN
• Three-color single-molecule imaging reveals conformational dynamics of dynein undergoing motility.
  Proceedings of the National Academy of Sciences of the United States of America 2021 Niekamp S, Stuurman N, Zhang N, Vale RD
• Pycro-Manager: open-source software for customized and reproducible microscope control.
  Nature methods 2021 Pinkard H, Stuurman N, Ivanov IE, Anthony NM, Ouyang W, Li B, Yang B, Tsuchida MA, Chhun B, Zhang G, Mei R, Anderson M, Shepherd DP, Hunt-Isaak I, Dunn RL, Jahr W, Kato S, Royer LA, Thiagarajah JR, …
• High-content imaging-based pooled CRISPR screens in mammalian cells.
  The Journal of cell biology 2021 Yan X, Stuurman N, Ribeiro SA, Tanenbaum ME, Horlbeck MA, Liem CR, Jost M, Weissman JS, Vale RD

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• A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging.
  Nature methods 2020 Niekamp S, Stuurman N, Vale RD
• Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution.
  Nature methods 2019 Yang B, Chen X, Wang Y, Feng S, Pessino V, Stuurman N, Cho NH, Cheng KW, Lord SJ, Xu L, Xie D, Mullins RD, Leonetti MD, Huang B
• Nanometer-accuracy distance measurements between fluorophores at the single-molecule level.
  Proceedings of the National Academy of Sciences of the United States of America 2019 Niekamp S, Sung J, Huynh W, Bhabha G, Vale RD, Stuurman N
• Cellular aspect ratio and cell division mechanics underlie the patterning of cell progeny in diverse mammalian epithelia.
  eLife 2018 McKinley KL, Stuurman N, Royer LA, Schartner C, Castillo-Azofeifa D, Delling M, Klein OD, Vale RD
• Visualizing Calcium Flux in Freely Moving Nematode Embryos.
  Biophysical journal 2017 Ardiel EL, Kumar A, Marbach J, Christensen R, Gupta R, Duncan W, Daniels JS, Stuurman N, Colón-Ramos D, Shroff H
• An acquisition and analysis pipeline for scanning angle interference microscopy.
  Nature methods 2016 Carbone CB, Vale RD, Stuurman N
• Micro-Magellan: open-source, sample-adaptive, acquisition software for optical microscopy.
  Nature methods 2016 Pinkard H, Stuurman N, Corbin K, Vale R, Krummel MF
• Impact of New Camera Technologies on Discoveries in Cell Biology.
  The Biological bulletin 2016 Stuurman N, Vale RD
• Quantitative evaluation of software packages for single-molecule localization microscopy.
  Nature methods 2015 Sage D, Kirshner H, Pengo T, Stuurman N, Min J, Manley S, Unser M
• High-resolution imaging of cardiomyocyte behavior reveals two distinct steps in ventricular trabeculation.
  Development (Cambridge, England) 2014 Staudt DW, Liu J, Thorn KS, Stuurman N, Liebling M, Stainier DY
• Advanced methods of microscope control using μManager software.
  Journal of biological methods 2014 Edelstein AD, Tsuchida MA, Amodaj N, Pinkard H, Vale RD, Stuurman N
• Genes involved in centrosome-independent mitotic spindle assembly in Drosophila S2 cells.
  Proceedings of the National Academy of Sciences of the United States of America 2013 Moutinho-Pereira S, Stuurman N, Afonso O, Hornsveld M, Aguiar P, Goshima G, Vale RD, Maiato H
• Chromophore-assisted laser inactivation in neural development.
  Neuroscience bulletin 2012 Li W, Stuurman N, Ou G
• Biological imaging software tools.
  Nature methods 2012 Eliceiri KW, Berthold MR, Goldberg IG, Ibáñez L, Manjunath BS, Martone ME, Murphy RF, Peng H, Plant AL, Roysam B, Stuurman N, Stuurmann N, Swedlow JR, Tomancak P, Carpenter AE
• Software tools, data structures, and interfaces for microscope imaging.
  Cold Spring Harbor protocols 2012 Stuurman N, Swedlow JR
• Computer control of microscopes using µManager.
  Current protocols in molecular biology 2010 Edelstein A, Amodaj N, Hoover K, Vale R, Stuurman N
• Polarized myosin produces unequal-size daughters during asymmetric cell division.
  Science (New York, N.Y.) 2010 Ou G, Stuurman N, D'Ambrosio M, Vale RD
• Determining single-molecule intensity as a function of power density.
  Cold Spring Harbor protocols 2010 Reck-Peterson SL, Derr ND, Stuurman N
• Imaging single molecular motor motility with total internal reflection fluorescence microscopy (TIRFM).
  Cold Spring Harbor protocols 2010 Reck-Peterson SL, Derr ND, Stuurman N
• Imaging single molecules using total internal reflection fluorescence microscopy (TIRFM).
  Cold Spring Harbor protocols 2010 Reck-Peterson SL, Derr ND, Stuurman N
• Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle.
  The Journal of cell biology 2008 Goshima G, Mayer M, Zhang N, Stuurman N, Vale RD
• Functional genomic screen reveals genes involved in lipid-droplet formation and utilization.
  Nature 2008 Guo Y, Walther TC, Rao M, Stuurman N, Goshima G, Terayama K, Wong JS, Vale RD, Walter P, Farese RV
• High throughput microscopy: from raw images to discoveries.
  Journal of cell science 2007 Wollman R, Stuurman N
• Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore.
  The Journal of cell biology 2007 Griffis ER, Stuurman N, Vale RD
• Genes required for mitotic spindle assembly in Drosophila S2 cells.
  Science (New York, N.Y.) 2007 Goshima G, Wollman R, Goodwin SS, Zhang N, Scholey JM, Vale RD, Stuurman N
• Single-molecule observations of neck linker conformational changes in the kinesin motor protein.
  Nature structural & molecular biology 2006 Tomishige M, Stuurman N, Vale RD
• Length control of the metaphase spindle.
  Current biology : CB 2005 Goshima G, Wollman R, Stuurman N, Scholey JM, Vale RD
• Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen.
  Proceedings of the National Academy of Sciences of the United States of America 2005 Cheng LW, Viala JP, Stuurman N, Wiedemann U, Vale RD, Portnoy DA
• Distinct pathways control recruitment and maintenance of myosin II at the cleavage furrow during cytokinesis.
  Proceedings of the National Academy of Sciences of the United States of America 2005 Dean SO, Rogers SL, Stuurman N, Vale RD, Spudich JA
• Molecular requirements for actin-based lamella formation in Drosophila S2 cells.
  The Journal of cell biology 2003 Rogers SL, Wiedemann U, Stuurman N, Vale RD
• Mitochondrial positioning in fission yeast is driven by association with dynamic microtubules and mitotic spindle poles.
  Proceedings of the National Academy of Sciences of the United States of America 2003 Yaffe MP, Stuurman N, Vale RD
• Role of phosphatidylinositol(4,5)bisphosphate organization in membrane transport by the Unc104 kinesin motor.
  Cell 2002 Klopfenstein DR, Tomishige M, Stuurman N, Vale RD
• Simultaneous imaging of Pseudomonas fluorescens WCS365 populations expressing three different autofluorescent proteins in the rhizosphere: new perspectives for studying microbial communities.
  Molecular plant-microbe interactions : MPMI 2000 Bloemberg GV, Wijfjes AH, Lamers GE, Stuurman N, Lugtenberg BJ
• Use of green fluorescent protein color variants expressed on stable broad-host-range vectors to visualize rhizobia interacting with plants.
  Molecular plant-microbe interactions : MPMI 2000 Stuurman N, Pacios Bras C, Schlaman HR, Wijfjes AH, Bloemberg G, Spaink HP
• B-type granule containing protrusions and interconnections between amyloplasts in developing wheat endosperm revealed by transmission electron microscopy and GFP expression.
  Journal of experimental botany 2000 Langeveld SM, van Wijk R, Stuurman N, Kijne JW, de Pater S
• A Lotus japonicus nodulation system based on heterologous expression of the fucosyl transferase NodZ and the acetyl transferase NoIL in Rhizobium leguminosarum.
  Molecular plant-microbe interactions : MPMI 2000 Pacios Bras C, Jordá MA, Wijfjes AH, Harteveld M, Stuurman N, Thomas-Oates JE, Spaink HP
• Ectopic overexpression of Drosophila lamin C is stage-specific lethal.
  Experimental cell research 1999 Stuurman N, Delbecque JP, Callaerts P, Aebi U
• Phosphorylation of the major Drosophila lamin in vivo: site identification during both M-phase (meiosis) and interphase by electrospray ionization tandem mass spectrometry.
  Biochemistry 1999 Schneider U, Mini T, Jenö P, Fisher PA, Stuurman N
• Interactions between coiled-coil proteins: Drosophila lamin Dm0 binds to the bicaudal-D protein.
  European journal of cell biology 1999 Stuurman N, Häner M, Sasse B, Hübner W, Suter B, Aebi U
• Assembly of Drosophila lamin Dm0 and C mutant proteins studied with the baculovirus system.
  European journal of cell biology 1998 Krohne G, Stuurman N, Kempf A
• A tailless Drosophila lamin Dm0 fragment reveals lateral associations of dimers.
  Journal of structural biology 1998 Sasse B, Aebi U, Stuurman N
• Interactions among Drosophila nuclear envelope proteins lamin, otefin, and YA.
  Molecular and cellular biology 1998 Goldberg M, Lu H, Stuurman N, Ashery-Padan R, Weiss AM, Yu J, Bhattacharyya D, Fisher PA, Gruenbaum Y, Wolfner MF
• In vivo association of lamins with nucleic acids in Drosophila melanogaster.
  Journal of cell science 1998 Rzepecki R, Bogachev SS, Kokoza E, Stuurman N, Fisher PA
• Nuclear lamins: their structure, assembly, and interactions.
  Journal of structural biology 1998 Stuurman N, Heins S, Aebi U
• In vitro assembly of Drosophila lamin Dm0--lamin polymerization properties are conserved.
  European journal of biochemistry 1997 Sasse B, Lustig A, Aebi U, Stuurman N
• Assembly of A- and B-type lamins studied in vivo with the baculovirus system.
  Journal of cell science 1997 Klapper M, Exner K, Kempf A, Gehrig C, Stuurman N, Fisher PA, Krohne G
• Identification of a conserved phosphorylation site modulating nuclear lamin polymerization.
  FEBS letters 1997 Stuurman N
• Intermediate filament protein polymerization: molecular analysis of Drosophila nuclear lamin head-to-tail binding.
  Journal of structural biology 1996 Stuurman N, Sasse B, Fisher PA
• DNA from Drosophila melanogaster beta-heterochromatin binds specifically to nuclear lamins in vitro and the nuclear envelope in situ.
  Gene 1996 Baricheva EA, Berrios M, Bogachev SS, Borisevich IV, Lapik ER, Sharakhov IV, Stuurman N, Fisher PA
• Binding of matrix attachment regions to nuclear lamin is mediated by the rod domain and depends on the lamin polymerization state.
  FEBS letters 1996 Zhao K, Harel A, Stuurman N, Guedalia D, Gruenbaum Y
• Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins.
  Journal of cell science 1995 Riemer D, Stuurman N, Berrios M, Hunter C, Fisher PA, Weber K
• Interphase phosphorylation of the Drosophila nuclear lamin: site-mapping using a monoclonal antibody.
  Journal of cell science 1995 Stuurman N, Maus N, Fisher PA
• Disassembly of the Drosophila nuclear lamina in a homologous cell-free system.
  Journal of cell science 1995 Maus N, Stuurman N, Fisher PA
• The t(15;17) translocation alters a nuclear body in a retinoic acid-reversible fashion.
  The EMBO journal 1994 Koken MH, Puvion-Dutilleul F, Guillemin MC, Viron A, Linares-Cruz G, Stuurman N, de Jong L, Szostecki C, Calvo F, Chomienne C
• Intranuclear filaments containing a nuclear pore complex protein.
  The Journal of cell biology 1993 Cordes VC, Reidenbach S, Köhler A, Stuurman N, van Driel R, Franke WW
• Induction of nuclear lamins A/C during in vitro-induced differentiation of F9 and P19 embryonal carcinoma cells.
  Experimental cell research 1992 Mattia E, Hoff WD, den Blaauwen J, Meijne AM, Stuurman N, van Renswoude J
• Nuclear frameworks: concepts and operational definitions.
  Cell biology international reports 1992 Stuurman N, de Jong L, van Driel R
• Three-dimensional immunogold labeling of nuclear matrix proteins in permeabilized cells.
  Cell biology international reports 1992 de Graaf A, Humbel BM, Stuurman N, van Bergen en Henegouwen PM, Verkleij AJ
• Stabilization of the nuclear matrix by disulfide bridges: identification of matrix polypeptides that form disulfides.
  Experimental cell research 1992 Stuurman N, Floore A, Colen A, de Jong L, van Driel R
• A monoclonal antibody recognizing nuclear matrix-associated nuclear bodies.
  Journal of cell science 1992 Stuurman N, de Graaf A, Floore A, Josso A, Humbel B, de Jong L, van Driel R
• Principles of nuclear organization.
  Cell biology international reports 1990 de Jong L, van Driel R, Stuurman N, Meijne AM, van Renswoude J
• The nuclear matrix from cells of different origin. Evidence for a common set of matrix proteins.
  The Journal of biological chemistry 1990 Stuurman N, Meijne AM, van der Pol AJ, de Jong L, van Driel R, van Renswoude J
• The protein composition of the nuclear matrix of murine P19 embryonal carcinoma cells is differentiation-stage dependent.
  Experimental cell research 1989 Stuurman N, Van Driel R, De Jong L, Meijne AM, Van Renswoude J