Michael McManus, PhD
Professor
Diabetes Center
School of Medicine

The McManus lab studies fundamental processes relating to the regulation of gene expression. We take high-throughput approaches, analyzing hundreds of thousands to millions of experiments at once, using unique and complex libraries coupled to deep sequencing. Our systems span from cell culture to in vivo models, focusing on a broad array of disease relevant tissues.

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From cancer to diabetes, we develop novel technologies to help us better understand how genes are regulated and how they function in cells. We aim to uncover the dark matter of the genome, to help unravel the beautiful genomic complexity of pathways and how genes interact in development and disease.

Websites

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• McManus Lab (mcmanuslab.ucsf.edu)
• RNA Journal Club (rnajc.ucsf.edu)
• The ViraCore at UCSF (viracore.ucsf.edu)
• Genomic Dark Matter (darkmatter.ucsf.edu)
• Bay Area RNA Club (barc.ucsf.edu)
• Cancer Target Discovery and Development (ctdd.ucsf.edu)
• Parnassus Center for Advanced Technologies (pcat.ucsf.edu)

Videos

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• UCSF Alumni Weekend (youtube.com)

Grants and Projects

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• Illuminating Druggable Dark Matter, NIH, 2017-2023
• The Cancer Target Discovery and Development Network at UCSF, NIH, 2017-2022
• Functional Networks for Persister Cell Sensitivities, NIH, 2017-2022
• Bay Area CRISPR Reporters, NIH, 2018-2021
• Diabetes Research Center, NIH, 2002-2021
• Regulation of developmental potency by the transposon LINE1, NIH/NIGMS, 2017-2021
• Tracing Cell Lineages, NIH, 2014-2019
• Empiric deconvolution of functional RNA elements, NIH, 2014-2019
• Genetic models for exRNA communication, NIH, 2013-2018
• Unlocking the Hidden Kinome, NIH, 2014-2017
• Bay Area Cancer Target Discovery and Development Network, NIH, 2012-2017
• New Frontiers for Small RNA Therapies, NIH, 2007-2014
• Whole genome RNA-interference screen for novel regulators of the insulin promoter, NIH, 2009-2012
• Resolving MicroRNA Targets, NIH, 2009-2011
• The Epigenetics of Small RNAs in the Mammalian Brain, NIH, 2006-2009

Publications (122)

Top publication keywords:
Lacrimal ApparatusCRISPR-Cas SystemsOrganogenesisDEAD-box RNA HelicasesEndoribonucleasesInsulin-Secreting CellsRNAMammalsGlutathione PeroxidaseEpistasis, GeneticRNA InterferenceMolecular TypingRibonuclease IIIRNA, Small InterferingMicroRNAs

• miR-200 deficiency promotes lung cancer metastasis by activating Notch signaling in cancer-associated fibroblasts.
  Genes & development 2021 Xue B, Chuang CH, Prosser HM, Fuziwara CS, Chan C, Sahasrabudhe N, Kühn M, Wu Y, Chen J, Biton A, Chen C, Wilkinson JE, McManus MT, Bradley A, Winslow MM, Su B, He L
• Examining the evidence for extracellular RNA function in mammals.
  Nature reviews. Genetics 2021 Gruner HN, McManus MT
• High-Throughput CRISPR Screening Identifies Genes Involved in Macrophage Viability and Inflammatory Pathways.
  Cell reports 2020 Covarrubias S, Vollmers AC, Capili A, Boettcher M, Shulkin A, Correa MR, Halasz H, Robinson EK, O'Briain L, Vollmers C, Blau J, Katzman S, McManus MT, Carpenter S
• miR-29 Sustains B Cell Survival and Controls Terminal Differentiation via Regulation of PI3K Signaling.
  Cell reports 2020 Hines MJ, Coffre M, Mudianto T, Panduro M, Wigton EJ, Tegla C, Osorio-Vasquez V, Kageyama R, Benhamou D, Perez O, Bajwa S, McManus MT, Ansel KM, Melamed D, Koralov SB
• Macrophage Exosomes Resolve Atherosclerosis by Regulating Hematopoiesis and Inflammation via MicroRNA Cargo.
  Cell reports 2020 Bouchareychas L, Duong P, Covarrubias S, Alsop E, Phu TA, Chung A, Gomes M, Wong D, Meechoovet B, Capili A, Yamamoto R, Nakauchi H, McManus MT, Carpenter S, Van Keuren-Jensen K, Raffai RL

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• CD81 Controls Beige Fat Progenitor Cell Growth and Energy Balance via FAK Signaling.
  Cell 2020 Oguri Y, Shinoda K, Kim H, Alba DL, Bolus WR, Wang Q, Brown Z, Pradhan RN, Tajima K, Yoneshiro T, Ikeda K, Chen Y, Cheang RT, Tsujino K, Kim CR, Greiner VJ, Datta R, Yang CD, Atabai K, McManus MT, …
• Host-Receptor Post-Translational Modifications Refine Staphylococcal Leukocidin Cytotoxicity.
  Toxins 2020 Tromp AT, Van Gent M, Jansen JP, Scheepmaker LM, Velthuizen A, De Haas CJC, Van Kessel KPM, Bardoel BW, Boettcher M, McManus MT, Van Strijp JAG, Lebbink RJ, Haas PA, Spaan AN
• BCAA catabolism in brown fat controls energy homeostasis through SLC25A44.
  Nature 2019 Yoneshiro T, Wang Q, Tajima K, Matsushita M, Maki H, Igarashi K, Dai Z, White PJ, McGarrah RW, Ilkayeva OR, Deleye Y, Oguri Y, Kuroda M, Ikeda K, Li H, Ueno A, Ohishi M, Ishikawa T, Kim K, Chen Y, …
• Genomic Resolution of DLX-Orchestrated Transcriptional Circuits Driving Development of Forebrain GABAergic Neurons.
  Cell reports 2019 Lindtner S, Catta-Preta R, Tian H, Su-Feher L, Price JD, Dickel DE, Greiner V, Silberberg SN, McKinsey GL, McManus MT, Pennacchio LA, Visel A, Nord AS, Rubenstein JLR
• miR-15/16 Restrain Memory T Cell Differentiation, Cell Cycle, and Survival.
  Cell reports 2019 Gagnon JD, Kageyama R, Shehata HM, Fassett MS, Mar DJ, Wigton EJ, Johansson K, Litterman AJ, Odorizzi P, Simeonov D, Laidlaw BJ, Panduro M, Patel S, Jeker LT, Feeney ME, McManus MT, Marson A, …
• Thermoregulation via Temperature-Dependent PGD2 Production in Mouse Preoptic Area.
  Neuron 2019 Wang TA, Teo CF, Åkerblom M, Chen C, Tynan-La Fontaine M, Greiner VJ, Diaz A, McManus MT, Jan YN, Jan LY
• Thermoregulation via Temperature-Dependent PGD2 Production in Mouse Preoptic Area.
  Neuron 2019 Wang TA, Teo CF, Åkerblom M, Chen C, Tynan-La Fontaine M, Greiner VJ, Diaz A, McManus MT, Jan YN, Jan LY
• High-Complexity shRNA Libraries and PI3 Kinase Inhibition in Cancer: High-Fidelity Synthetic Lethality Predictions.
  Cell reports 2019 Mues M, Karra L, Romero-Moya D, Wandler A, Hangauer MJ, Ksionda O, Thus Y, Lindenbergh M, Shannon K, McManus MT, Roose JP
• The Extracellular RNA Communication Consortium: Establishing Foundational Knowledge and Technologies for Extracellular RNA Research.
  Cell 2019 Das S, Extracellular RNA Communication Consortium, Ansel KM, Bitzer M, Breakefield XO, Charest A, Galas DJ, Gerstein MB, Gupta M, Milosavljevic A, McManus MT, Patel T, Raffai RL, Rozowsky J, Roth ME, …
• Tracing cellular heterogeneity in pooled genetic screens via multi-level barcoding.
  BMC genomics 2019 Boettcher M, Covarrubias S, Biton A, Blau J, Wang H, Zaitlen N, McManus MT
• Far away from the lamppost.
  PLoS biology 2018 Oprea TI, Jan L, Johnson GL, Roth BL, Ma'ayan A, Schürer S, Shoichet BK, Sklar LA, McManus MT
• Genetic Models Reveal cis and trans Immune-Regulatory Activities for lincRNA-Cox2.
  Cell reports 2018 Elling R, Robinson EK, Shapleigh B, Liapis SC, Covarrubias S, Katzman S, Groff AF, Jiang Z, Agarwal S, Motwani M, Chan J, Sharma S, Hennessy EJ, FitzGerald GA, McManus MT, Rinn JL, Fitzgerald KA, …
• miR-205 Regulates Basal Cell Identity and Stem Cell Regenerative Potential During Mammary Reconstitution.
  Stem cells (Dayton, Ohio) 2018 Lu Y, Cao J, Napoli M, Xia Z, Zhao N, Creighton CJ, Li W, Chen X, Flores ER, McManus MT, Rosen JM
• Publisher Correction: Human CD45 is an F-component-specific receptor for the staphylococcal toxin Panton-Valentine leukocidin.
  Nature microbiology 2018 Tromp AT, Van Gent M, Abrial P, Martin A, Jansen JP, De Haas CJC, Van Kessel KPM, Bardoel BW, Kruse E, Bourdonnay E, Boettcher M, McManus MT, Day CJ, Jennings MP, Lina G, Vandenesch F, Van Strijp JAG,…
• A Genetic Interaction Map of Insulin Production Identifies Mfi as an Inhibitor of Mitochondrial Fission.
  Endocrinology 2018 Lee J, Pappalardo Z, Chopra DG, Hennings TG, Vaughn I, Lan C, Choe JJ, Ang K, Chen S, Arkin M, McManus MT, German MS, Ku GM
• Human CD45 is an F-component-specific receptor for the staphylococcal toxin Panton-Valentine leukocidin.
  Nature microbiology 2018 Tromp AT, Van Gent M, Abrial P, Martin A, Jansen JP, De Haas CJC, Van Kessel KPM, Bardoel BW, Kruse E, Bourdonnay E, Boettcher M, McManus MT, Day CJ, Jennings MP, Lina G, Vandenesch F, Van Strijp JAG,…
• Corrigendum: A systematic comparison reveals substantial differences in chromosomal versus episomal encoding of enhancer activity.
  Genome research 2018 Inoue F, Kircher M, Martin B, Cooper GM, Witten DM, McManus MT, Ahituv N, Shendure J
• Genome-wide CRISPR screen identifies FAM49B as a key regulator of actin dynamics and T cell activation.
  Proceedings of the National Academy of Sciences of the United States of America 2018 Shang W, Jiang Y, Boettcher M, Ding K, Mollenauer M, Liu Z, Wen X, Liu C, Hao P, Zhao S, McManus MT, Wei L, Weiss A, Wang H
• Unexplored therapeutic opportunities in the human genome.
  Nature reviews. Drug discovery 2018 Oprea TI, Bologa CG, Brunak S, Campbell A, Gan GN, Gaulton A, Gomez SM, Guha R, Hersey A, Holmes J, Jadhav A, Jensen LJ, Johnson GL, Karlson A, Leach AR, Ma'ayan A, Malovannaya A, Mani S, Mathias SL, …
• Unexplored therapeutic opportunities in the human genome.
  Nature reviews. Drug discovery 2018 Oprea TI, Bologa CG, Brunak S, Campbell A, Gan GN, Gaulton A, Gomez SM, Guha R, Hersey A, Holmes J, Jadhav A, Jensen LJ, Johnson GL, Karlson A, Leach AR, Ma'ayan A, Malovannaya A, Mani S, Mathias SL, …
• The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output.
  Cell stem cell 2018 Bulut-Karslioglu A, Macrae TA, Oses-Prieto JA, Covarrubias S, Percharde M, Ku G, Diaz A, McManus MT, Burlingame AL, Ramalho-Santos M
• Dual Strategies for Argonaute2-Mediated Biogenesis of Erythroid miRNAs Underlie Conserved Requirements for Slicing in Mammals.
  Molecular cell 2018 Jee D, Yang JS, Park SM, Farmer DT, Wen J, Chou T, Chow A, McManus MT, Kharas MG, Lai EC
• Dual gene activation and knockout screen reveals directional dependencies in genetic networks.
  Nature biotechnology 2018 Boettcher M, Tian R, Blau JA, Markegard E, Wagner RT, Wu D, Mo X, Biton A, Zaitlen N, Fu H, McCormick F, Kampmann M, McManus MT
• Drug-tolerant persister cancer cells are vulnerable to GPX4 inhibition.
  Nature 2017 Hangauer MJ, Viswanathan VS, Ryan MJ, Bole D, Eaton JK, Matov A, Galeas J, Dhruv HD, Berens ME, Schreiber SL, McCormick F, McManus MT
• CRISPR/Cas-based screening of long non-coding RNAs (lncRNAs) in macrophages with an NF-κB reporter.
  The Journal of biological chemistry 2017 Covarrubias S, Robinson EK, Shapleigh B, Vollmers A, Katzman S, Hanley N, Fong N, McManus MT, Carpenter S
• Defining epithelial cell dynamics and lineage relationships in the developing lacrimal gland.
  Development (Cambridge, England) 2017 Farmer DT, Nathan S, Finley JK, Shengyang Yu K, Emmerson E, Byrnes LE, Sneddon JB, McManus MT, Tward AD, Knox SM
• miR-205 is a critical regulator of lacrimal gland development.
  Developmental biology 2017 Farmer DT, Finley JK, Chen FY, Tarifeño-Saldivia E, McNamara NA, Knox SM, McManus MT
• A Whole-Genome RNA Interference Screen Reveals a Role for Spry2 in Insulin Transcription and the Unfolded Protein Response.
  Diabetes 2017 Pappalardo Z, Gambhir Chopra D, Hennings TG, Richards H, Choe J, Yang K, Baeyens L, Ang K, Chen S, Arkin M, German MS, McManus MT, Ku GM
• MicroRNAs in ectodermal appendages.
  Current opinion in genetics & development 2017 Farmer DT, McManus MT
• A systematic comparison reveals substantial differences in chromosomal versus episomal encoding of enhancer activity.
  Genome research 2016 Inoue F, Kircher M, Martin B, Cooper GM, Witten DM, McManus MT, Ahituv N, Shendure J
• PIM1 kinase inhibition as a targeted therapy against triple-negative breast tumors with elevated MYC expression.
  Nature medicine 2016 Horiuchi D, Camarda R, Zhou AY, Yau C, Momcilovic O, Balakrishnan S, Corella AN, Eyob H, Kessenbrock K, Lawson DA, Marsh LA, Anderton BN, Rohrberg J, Kunder R, Bazarov AV, Yaswen P, McManus MT, Rugo …
• MicroRNAs 24 and 27 Suppress Allergic Inflammation and Target a Network of Regulators of T Helper 2 Cell-Associated Cytokine Production.
  Immunity 2016 Pua HH, Steiner DF, Patel S, Gonzalez JR, Ortiz-Carpena JF, Kageyama R, Chiou NT, Gallman A, de Kouchkovsky D, Jeker LT, McManus MT, Erle DJ, Ansel KM
• Biogenesis, delivery, and function of extracellular RNA.
  Journal of extracellular vesicles 2015 Patton JG, Franklin JL, Weaver AM, Vickers K, Zhang B, Coffey RJ, Ansel KM, Blelloch R, Goga A, Huang B, L'Etoille N, Raffai RL, Lai CP, Krichevsky AM, Mateescu B, Greiner VJ, Hunter C, Voinnet O, …
• Identification of MiR-205 As a MicroRNA That Is Highly Expressed in Medullary Thymic Epithelial Cells.
  PloS one 2015 Khan IS, Park CY, Mavropoulos A, Shariat N, Pollack JL, Barczak AJ, Erle DJ, McManus MT, Anderson MS, Jeker LT
• Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR.
  Molecular cell 2015 Boettcher M, McManus MT
• Integrative analysis of 111 reference human epigenomes.
  Nature 2015 Roadmap Epigenomics Consortium, Kundaje A, Meuleman W, Ernst J, Bilenky M, Yen A, Heravi-Moussavi A, Kheradpour P, Zhang Z, Wang J, Ziller MJ, Amin V, Whitaker JW, Schultz MD, Ward LD, Sarkar A, Quon …
• Epigenetic and transcriptional determinants of the human breast.
  Nature communications 2015 Gascard P, Bilenky M, Sigaroudinia M, Zhao J, Li L, Carles A, Delaney A, Tam A, Kamoh B, Cho S, Griffith M, Chu A, Robertson G, Cheung D, Li I, Heravi-Moussavi A, Moksa M, Mingay M, Hussainkhel A, …
• A screen in mice uncovers repression of lipoprotein lipase by microRNA-29a as a mechanism for lipid distribution away from the liver.
  Hepatology (Baltimore, Md.) 2014 Mattis AN, Song G, Hitchner K, Kim RY, Lee AY, Sharma AD, Malato Y, McManus MT, Esau CC, Koller E, Koliwad S, Lim LP, Maher JJ, Raffai RL, Willenbring H
• Systematic identification of barriers to human iPSC generation.
  Cell 2014 Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust EM, McManus MT, Song JS, Ramalho-Santos M
• A high-coverage shRNA screen identifies TMEM129 as an E3 ligase involved in ER-associated protein degradation.
  Nature communications 2014 van de Weijer ML, Bassik MC, Luteijn RD, Voorburg CM, Lohuis MA, Kremmer E, Hoeben RC, LeProust EM, Chen S, Hoelen H, Ressing ME, Patena W, Weissman JS, McManus MT, Wiertz EJ, Lebbink RJ
• Discovering the complexity of the metazoan transcriptome.
  Genome biology 2014 Hangauer MJ, Carpenter S, McManus MT
• Massively parallel functional annotation of 3' untranslated regions.
  Nature biotechnology 2014 Zhao W, Pollack JL, Blagev DP, Zaitlen N, McManus MT, Erle DJ
• Negative regulation of Hif1a expression and TH17 differentiation by the hypoxia-regulated microRNA miR-210.
  Nature immunology 2014 Wang H, Flach H, Onizawa M, Wei L, McManus MT, Weiss A
• Next-generation NAMPT inhibitors identified by sequential high-throughput phenotypic chemical and functional genomic screens.
  Chemistry & biology 2013 Matheny CJ, Wei MC, Bassik MC, Donnelly AJ, Kampmann M, Iwasaki M, Piloto O, Solow-Cordero DE, Bouley DM, Rau R, Brown P, McManus MT, Weissman JS, Cleary ML
• Partially penetrant postnatal lethality of an epithelial specific MicroRNA in a mouse knockout.
  PloS one 2013 Farmer DT, Shariat N, Park CY, Liu HJ, Mavropoulos A, McManus MT
• The Pitx2:miR-200c/141:noggin pathway regulates Bmp signaling and ameloblast differentiation.
  Development (Cambridge, England) 2013 Cao H, Jheon A, Li X, Sun Z, Wang J, Florez S, Zhang Z, McManus MT, Klein OD, Amendt BA
• Pervasive transcription of the human genome produces thousands of previously unidentified long intergenic noncoding RNAs.
  PLoS genetics 2013 Hangauer MJ, Vaughn IW, McManus MT
• Renewable RNAi.
  Nature biotechnology 2013 Blau JA, McManus MT
• A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility.
  Cell 2013 Bassik MC, Kampmann M, Lebbink RJ, Wang S, Hein MY, Poser I, Weibezahn J, Horlbeck MA, Chen S, Mann M, Hyman AA, Leproust EM, McManus MT, Weissman JS
• T cell activation induces proteasomal degradation of Argonaute and rapid remodeling of the microRNA repertoire.
  The Journal of experimental medicine 2013 Bronevetsky Y, Villarino AV, Eisley CJ, Barbeau R, Barczak AJ, Heinz GA, Kremmer E, Heissmeyer V, McManus MT, Erle DJ, Rao A, Ansel KM
• IRE1α cleaves select microRNAs during ER stress to derepress translation of proapoptotic Caspase-2.
  Science (New York, N.Y.) 2012 Upton JP, Wang L, Han D, Wang ES, Huskey NE, Lim L, Truitt M, McManus MT, Ruggero D, Goga A, Papa FR, Oakes SA
• Research resource: RNA-Seq reveals unique features of the pancreatic β-cell transcriptome.
  Molecular endocrinology (Baltimore, Md.) 2012 Ku GM, Kim H, Vaughn IW, Hangauer MJ, Myung Oh C, German MS, McManus MT
• Let-7b/c enhance the stability of a tissue-specific mRNA during mammalian organogenesis as part of a feedback loop involving KSRP.
  PLoS genetics 2012 Repetto E, Briata P, Kuziner N, Harfe BD, McManus MT, Gherzi R, Rosenfeld MG, Trabucchi M
• Right- and left-loop short shRNAs have distinct and unusual mechanisms of gene silencing.
  Nucleic acids research 2012 Dallas A, Ilves H, Ge Q, Kumar P, Shorenstein J, Kazakov SA, Cuellar TL, McManus MT, Behlke MA, Johnston BH
• A resource for the conditional ablation of microRNAs in the mouse.
  Cell reports 2012 Park CY, Jeker LT, Carver-Moore K, Oh A, Liu HJ, Cameron R, Richards H, Li Z, Adler D, Yoshinaga Y, Martinez M, Nefadov M, Abbas AK, Weiss A, Lanier LL, de Jong PJ, Bluestone JA, Srivastava D, McManus…
• Precursor microRNA-programmed silencing complex assembly pathways in mammals.
  Molecular cell 2012 Liu X, Jin DY, McManus MT, Mourelatos Z
• Widespread RNA 3'-end oligouridylation in mammals.
  RNA (New York, N.Y.) 2012 Choi YS, Patena W, Leavitt AD, McManus MT
• An siRNA screen in pancreatic beta cells reveals a role for Gpr27 in insulin production.
  PLoS genetics 2012 Ku GM, Pappalardo Z, Luo CC, German MS, McManus MT
• Polymerase II promoter strength determines efficacy of microRNA adapted shRNAs.
  PloS one 2011 Lebbink RJ, Lowe M, Chan T, Khine H, Wang X, McManus MT
• The microRNA-processing enzyme Dicer is dispensable for somite segmentation but essential for limb bud positioning.
  Developmental biology 2011 Zhang Z, O'Rourke JR, McManus MT, Lewandoski M, Harfe BD, Sun X
• Posttranscriptional silencing of effector cytokine mRNA underlies the anergic phenotype of self-reactive T cells.
  Immunity 2011 Villarino AV, Katzman SD, Gallo E, Miller O, Jiang S, McManus MT, Abbas AK
• Analysis of microRNA knockouts in mice.
  Human molecular genetics 2010 Park CY, Choi YS, McManus MT
• Dicer1 and miR-219 Are required for normal oligodendrocyte differentiation and myelination.
  Neuron 2010 Dugas JC, Cuellar TL, Scholze A, Ason B, Ibrahim A, Emery B, Zamanian JL, Foo LC, McManus MT, Barres BA
• Dicer ablation in oligodendrocytes provokes neuronal impairment in mice.
  Annals of neurology 2009 Shin D, Shin JY, McManus MT, Ptácek LJ, Fu YH
• Expanded RNA-binding activities of mammalian Argonaute 2.
  Nucleic acids research 2009 Tan GS, Garchow BG, Liu X, Yeung J, Morris JP, Cuellar TL, McManus MT, Kiriakidou M
• Dicer is required for proper liver zonation.
  The Journal of pathology 2009 Sekine S, Ogawa R, Mcmanus MT, Kanai Y, Hebrok M
• Lentivirus production.
  Journal of visualized experiments : JoVE 2009 Wang X, McManus M
• Chd1 regulates open chromatin and pluripotency of embryonic stem cells.
  Nature 2009 Gaspar-Maia A, Alajem A, Polesso F, Sridharan R, Mason MJ, Heidersbach A, Ramalho-Santos J, McManus MT, Plath K, Meshorer E, Ramalho-Santos M
• Rapid creation and quantitative monitoring of high coverage shRNA libraries.
  Nature methods 2009 Bassik MC, Lebbink RJ, Churchman LS, Ingolia NT, Patena W, LeProust EM, Schuldiner M, Weissman JS, McManus MT
• LPS induces KH-type splicing regulatory protein-dependent processing of microRNA-155 precursors in macrophages.
  FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Ruggiero T, Trabucchi M, De Santa F, Zupo S, Harfe BD, McManus MT, Rosenfeld MG, Briata P, Gherzi R
• Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion.
  The Journal of biological chemistry 2009 Huang TH, Wu F, Loeb GB, Hsu R, Heidersbach A, Brincat A, Horiuchi D, Lebbink RJ, Mo YY, Goga A, McManus MT
• Disruption of Dicer1 induces dysregulated fetal gene expression and promotes hepatocarcinogenesis.
  Gastroenterology 2009 Sekine S, Ogawa R, Ito R, Hiraoka N, McManus MT, Kanai Y, Hebrok M
• Characterization of the 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase multigene family of Malus domestica Borkh.
  Phytochemistry 2009 Binnie JE, McManus MT
• Residual microRNA expression dictates the extent of inner ear development in conditional Dicer knockout mice.
  Developmental biology 2009 Soukup GA, Fritzsch B, Pierce ML, Weston MD, Jahan I, McManus MT, Harfe BD
• Genetic analyses reveal a requirement for Dicer1 in the mouse urogenital tract.
  Mammalian genome : official journal of the International Mammalian Genome Society 2009 Pastorelli LM, Wells S, Fray M, Smith A, Hough T, Harfe BD, McManus MT, Smith L, Woolf AS, Cheeseman M, Greenfield A
• Sertoli cell Dicer is essential for spermatogenesis in mice.
  Developmental biology 2008 Papaioannou MD, Pitetti JL, Ro S, Park C, Aubry F, Schaad O, Vejnar CE, Kühne F, Descombes P, Zdobnov EM, McManus MT, Guillou F, Harfe BD, Yan W, Jégou B, Nef S
• Podocyte-specific deletion of dicer alters cytoskeletal dynamics and causes glomerular disease.
  Journal of the American Society of Nephrology : JASN 2008 Harvey SJ, Jarad G, Cunningham J, Goldberg S, Schermer B, Harfe BD, McManus MT, Benzing T, Miner JH
• Selective miRNA disruption in T reg cells leads to uncontrolled autoimmunity.
  The Journal of experimental medicine 2008 Zhou X, Jeker LT, Fife BT, Zhu S, Anderson MS, McManus MT, Bluestone JA
• Dicer1 is required for differentiation of the mouse male germline.
  Biology of reproduction 2008 Maatouk DM, Loveland KL, McManus MT, Moore K, Harfe BD
• Mouse let-7 miRNA populations exhibit RNA editing that is constrained in the 5'-seed/ cleavage/anchor regions and stabilize predicted mmu-let-7a:mRNA duplexes.
  Genome research 2008 Reid JG, Nagaraja AK, Lynn FC, Drabek RB, Muzny DM, Shaw CA, Weiss MK, Naghavi AO, Khan M, Zhu H, Tennakoon J, Gunaratne GH, Corry DB, Miller J, McManus MT, German MS, Gibbs RA, Matzuk MM, Gunaratne …
• Dicer inactivation leads to progressive functional and structural degeneration of the mouse retina.
  The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Damiani D, Alexander JJ, O'Rourke JR, McManus M, Jadhav AP, Cepko CL, Hauswirth WW, Harfe BD, Strettoi E
• Conditional loss of Dicer disrupts cellular and tissue morphogenesis in the cortex and hippocampus.
  The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Davis TH, Cuellar TL, Koch SM, Barker AJ, Harfe BD, McManus MT, Ullian EM
• Dicer loss in striatal neurons produces behavioral and neuroanatomical phenotypes in the absence of neurodegeneration.
  Proceedings of the National Academy of Sciences of the United States of America 2008 Cuellar TL, Davis TH, Nelson PT, Loeb GB, Harfe BD, Ullian E, McManus MT
• Michael T. McManus: Interrupting biology [interview by Hema Bashyam].
  The Journal of experimental medicine 2008 McManus MT
• Unintentional miRNA ablation is a risk factor in gene knockout studies: a short report.
  PLoS genetics 2008 Osokine I, Hsu R, Loeb GB, McManus MT
• Members of the miRNA-200 family regulate olfactory neurogenesis.
  Neuron 2008 Choi PS, Zakhary L, Choi WY, Caron S, Alvarez-Saavedra E, Miska EA, McManus M, Harfe B, Giraldez AJ, Horvitz HR, Schier AF, Dulac C
• Behind the scenes of a small RNA gene-silencing pathway.
  Human gene therapy 2008 Ku G, McManus MT
• Further examination of abscission zone cells as ethylene target cells in higher plants.
  Annals of botany 2007 McManus MT
• MicroRNA expression is required for pancreatic islet cell genesis in the mouse.
  Diabetes 2007 Lynn FC, Skewes-Cox P, Kosaka Y, McManus MT, Harfe BD, German MS
• Essential role for Dicer during skeletal muscle development.
  Developmental biology 2007 O'Rourke JR, Georges SA, Seay HR, Tapscott SJ, McManus MT, Goldhamer DJ, Swanson MS, Harfe BD
• Complex formation between recombinant ATP sulfurylase and APS reductase of Allium cepa (L.).
  FEBS letters 2007 Cumming M, Leung S, McCallum J, McManus MT
• Extracellular sulfatases, elements of the Wnt signaling pathway, positively regulate growth and tumorigenicity of human pancreatic cancer cells.
  PloS one 2007 Nawroth R, van Zante A, Cervantes S, McManus M, Hebrok M, Rosen SD
• Lentiviral strategies for RNAi knockdown of neuronal genes.
  Current protocols in neuroscience 2007 Crittenden JR, Heidersbach A, McManus MT
• Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2.
  Cell 2007 Zhao Y, Ransom JF, Li A, Vedantham V, von Drehle M, Muth AN, Tsuchihashi T, McManus MT, Schwartz RJ, Srivastava D
• Duox2 exhibits potent heme peroxidase activity in human respiratory tract epithelium.
  FEBS letters 2006 Harper RW, Xu C, McManus M, Heidersbach A, Eiserich JP
• RNA interference in embryonic stem cells and the prospects for future therapies.
  Gene therapy 2006 Heidersbach A, Gaspar-Maia A, McManus MT, Ramalho-Santos M
• Dicer function is essential for lung epithelium morphogenesis.
  Proceedings of the National Academy of Sciences of the United States of America 2006 Harris KS, Zhang Z, McManus MT, Harfe BD, Sun X
• MicroRNAs and endocrine biology.
  The Journal of endocrinology 2005 Cuellar TL, McManus MT
• The microRNA miR-196 acts upstream of Hoxb8 and Shh in limb development.
  Nature 2005 Hornstein E, Mansfield JH, Yekta S, Hu JK, Harfe BD, McManus MT, Baskerville S, Bartel DP, Tabin CJ
• Slowing down the Ras lane: miRNAs as tumor suppressors?
  Science's STKE : signal transduction knowledge environment 2005 Morris JP, McManus MT
• TAZ, a transcriptional modulator of mesenchymal stem cell differentiation.
  Science (New York, N.Y.) 2005 Hong JH, Hwang ES, McManus MT, Amsterdam A, Tian Y, Kalmukova R, Mueller E, Benjamin T, Spiegelman BM, Sharp PA, Hopkins N, Yaffe MB
• The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb.
  Proceedings of the National Academy of Sciences of the United States of America 2005 Harfe BD, McManus MT, Mansfield JH, Hornstein E, Tabin CJ
• Small RNAs and immunity.
  Immunity 2004 McManus MT
• MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression.
  Nature genetics 2004 Mansfield JH, Harfe BD, Nissen R, Obenauer J, Srineel J, Chaudhuri A, Farzan-Kashani R, Zuker M, Pasquinelli AE, Ruvkun G, Sharp PA, Tabin CJ, McManus MT
• Cre-lox-regulated conditional RNA interference from transgenes.
  Proceedings of the National Academy of Sciences of the United States of America 2004 Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L, Jaenisch R, Jacks T
• MicroRNAs and cancer.
  Seminars in cancer biology 2003 McManus MT
• RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription.
  Proceedings of the National Academy of Sciences of the United States of America 2003 Ge Q, McManus MT, Nguyen T, Shen CH, Sharp PA, Eisen HN, Chen J
• A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference.
  Nature genetics 2003 Rubinson DA, Dillon CP, Kwiatkowski AV, Sievers C, Yang L, Kopinja J, Rooney DL, Zhang M, Ihrig MM, McManus MT, Gertler FB, Scott ML, Van Parijs L
• Small interfering RNA-mediated gene silencing in T lymphocytes.
  Journal of immunology (Baltimore, Md. : 1950) 2002 McManus MT, Haines BB, Dillon CP, Whitehurst CE, van Parijs L, Chen J, Sharp PA
• Gene silencing in mammals by small interfering RNAs.
  Nature reviews. Genetics 2002 McManus MT, Sharp PA
• CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells.
  Developmental cell 2002 Chen Z, Indjeian VB, McManus M, Wang L, Dynlacht BD
• Gene silencing using micro-RNA designed hairpins.
  RNA (New York, N.Y.) 2002 McManus MT, Petersen CP, Haines BB, Chen J, Sharp PA
• Identification of candidate mitochondrial RNA editing ligases from Trypanosoma brucei.
  RNA (New York, N.Y.) 2001 McManus MT, Shimamura M, Grams J, Hajduk SL
• Processing of polycistronic guide RNAs is associated with RNA editing complexes in Trypanosoma brucei.
  The EMBO journal 2000 Grams J, McManus MT, Hajduk SL
• Trypanosoma brucei guide RNA poly(U) tail formation is stabilized by cognate mRNA.
  Molecular and cellular biology 2000 McManus MT, Adler BK, Pollard VW, Hajduk SL
• Biochemical methods for analysis of kinetoplastid RNA editing.
  Methods (San Diego, Calif.) 1998 Sabatini RS, Adler BK, Madison-Antenucci S, McManus MT, Hajduk SL
• Insertional and deletional RNA editing in trypanosome mitochondria.
  Nucleic acids symposium series 1997 Hajduk SL, Adler B, Madison-Antenucci S, McManus M, Sabatini R