Maxence Nachury, PhD
Professor
Ophthalmology
School of Medicine

The BBSome is an evolutionarily conserved complex of 8 BBS proteins that traffics signaling receptors out of cilia and we aim to decipher the molecular details of BBSome-mediated trafficking and the cellular, biochemical and structural level. A second research direction is aimed at cilia-derived extracellular vesicles (EVs). Ciliary signaling receptors that fail conventional exit are packaged into ciliary EVs. By dissecting the mechanisms of ciliary EV biogenesis, we will gain insight into the roles of these extracellular parcels in cell-cell communication.

• The gymnastics of intraflagellar transport complexes keeps trains running inside cilia.
  Cell 2022 Nachury MV
• Shedding of ciliary vesicles at a glance.
  Journal of cell science 2022 Ojeda Naharros I, Nachury MV
• Localization of GFP-Tagged Proteins Under the Electron Microscope.
  2021 Sara Gil-Perotin, A. Cebrián-Silla, V. Herranz-Pérez, P. García-Belda, S. Gil-García, M. Fil, J. S. Lee, M. V. Nachury, José Manuel García-Verdugo
• Time-resolved proteomics profiling of the ciliary Hedgehog response.
  The Journal of cell biology 2021 May EA, Kalocsay M, D'Auriac IG, Schuster PS, Gygi SP, Nachury MV, Mick DU
• Ubiquitin chains earmark GPCRs for BBSome-mediated removal from cilia.
  The Journal of cell biology 2020 Shinde SR, Nager AR, Nachury MV
• Near-atomic structures of the BBSome reveal the basis for BBSome activation and binding to GPCR cargoes.
  eLife 2020 Yang S, Bahl K, Chou HT, Woodsmith J, Stelzl U, Walz T, Nachury MV
• The Molecular Architecture of Native BBSome Obtained by an Integrated Structural Approach.
  Structure (London, England : 1993) 2019 Chou HT, Apelt L, Farrell DP, White SR, Woodsmith J, Svetlov V, Goldstein JS, Nager AR, Li Z, Muller J, Dollfus H, Nudler E, Stelzl U, DiMaio F, Nachury MV, Walz T
• Establishing and regulating the composition of cilia for signal transduction.
  Nature reviews. Molecular cell biology 2019 Nachury MV, Mick DU
• Effects of α-tubulin acetylation on microtubule structure and stability.
  Proceedings of the National Academy of Sciences of the United States of America 2019 Eshun-Wilson L, Zhang R, Portran D, Nachury MV, Toso DB, Löhr T, Vendruscolo M, Bonomi M, Fraser JS, Nogales E
• The molecular machines that traffic signaling receptors into and out of cilia.
  Current opinion in cell biology 2018 Nachury MV
• BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone.
  The Journal of cell biology 2018 Ye F, Nager AR, Nachury MV
• A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.
  Nature genetics 2018 Breslow DK, Hoogendoorn S, Kopp AR, Morgens DW, Vu BK, Kennedy MC, Han K, Li A, Hess GT, Bassik MC, Chen JK, Nachury MV
• BORC Regulates the Axonal Transport of Synaptic Vesicle Precursors by Activating ARL-8.
  Current biology : CB 2017 Niwa S, Tao L, Lu SY, Liew GM, Feng W, Nachury MV, Shen K
• Chemical structure-guided design of dynapyrazoles, cell-permeable dynein inhibitors with a unique mode of action.
  eLife 2017 Steinman JB, Santarossa CC, Miller RM, Yu LS, Serpinskaya AS, Furukawa H, Morimoto S, Tanaka Y, Nishitani M, Asano M, Zalyte R, Ondrus AE, Johnson AG, Ye F, Nachury MV, Fukase Y, Aso K, Foley MA, …
• Microtubules acquire resistance from mechanical breakage through intralumenal acetylation.
  Science (New York, N.Y.) 2017 Xu Z, Schaedel L, Portran D, Aguilar A, Gaillard J, Marinkovich MP, Théry M, Nachury MV
• Membrane traffic control by cytoskeletal and molecular machines.
  Molecular biology of the cell 2017 Lee TH, Nachury MV
• Fifteen years of research on oral-facial-digital syndromes: from 1 to 16 causal genes.
  Journal of medical genetics 2017 Bruel AL, Franco B, Duffourd Y, Thevenon J, Jego L, Lopez E, Deleuze JF, Doummar D, Giles RH, Johnson CA, Huynen MA, Chevrier V, Burglen L, Morleo M, Desguerres I, Pierquin G, Doray B, Gilbert-…
• Measurement of tubulin oligomers self-assembly by FRET.
  Protocol Exchange 2017 Didier Portran, Didier Portran, Maxence Nachury
• Tubulin acetylation protects long-lived microtubules against mechanical ageing.
  Nature cell biology 2017 Portran D, Schaedel L, Xu Z, Théry M, Nachury MV
• An Actin Network Dispatches Ciliary GPCRs into Extracellular Vesicles to Modulate Signaling.
  Cell 2016 Nager AR, Goldstein JS, Herranz-Pérez V, Portran D, Ye F, Garcia-Verdugo JM, Nachury MV
• Abstract B068: Structural basis for Notch1 engagement of Delta-like 4 and Jagged1.
  Immune Regulation of T Cell Responses/ Evolving Concepts in Cancer Immunology 2016 Vincent C. Luca, Kevin M. Jude, Nathan W. Pierce, Maxence V. Nachury, Suzanne Fischer, K. Christopher Garcia
• Localization of GFP-Tagged Proteins at the Electron Microscope.
  Receptor and Ion Channel Detection in the Brain 2016 Sara Gil-Perotin, A. Cebrián-Silla, V. Herranz-Pérez, P. García-Belda, S. Gil-García, M. Fil, J. S. Lee, M. V. Nachury, José Manuel García-Verdugo
• Loss of the BBSome perturbs endocytic trafficking and disrupts virulence of Trypanosoma brucei.
  Proceedings of the National Academy of Sciences of the United States of America 2015 Langousis G, Shimogawa MM, Saada EA, Vashisht AA, Spreafico R, Nager AR, Barshop WD, Nachury MV, Wohlschlegel JA, Hill KL
• The Intraflagellar Transport Protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3.
  Cilia 2015 G Liew, F Ye, A Nager, J Murphy, J Lee, M Aguiar, D Breslow, S Gygi, M Nachury
• Cytoplasmic Dynein Antagonists with Improved Potency and Isoform Selectivity.
  ACS chemical biology 2015 See SK, Hoogendoorn S, Chung AH, Ye F, Steinman JB, Sakata-Kato T, Miller RM, Cupido T, Zalyte R, Carter AP, Nachury MV, Kapoor TM, Chen JK
• Microtubules self-repair in response to mechanical stress.
  Nature materials 2015 Schaedel L, John K, Gaillard J, Nachury MV, Blanchoin L, Théry M
• Structural biology. Structural basis for Notch1 engagement of Delta-like 4.
  Science (New York, N.Y.) 2015 Luca VC, Jude KM, Pierce NW, Nachury MV, Fischer S, Garcia KC
• Analysis of soluble protein entry into primary cilia using semipermeabilized cells.
  Methods in cell biology 2015 Breslow DK, Nachury MV
• Structural basis for membrane targeting of the BBSome by ARL6.
  Nature structural & molecular biology 2014 Mourão A, Nager AR, Nachury MV, Lorentzen E
• The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3.
  Developmental cell 2014 Liew GM, Ye F, Nager AR, Murphy JP, Lee JS, Aguiar M, Breslow DK, Gygi SP, Nachury MV
• How do cilia organize signalling cascades?
  Philosophical transactions of the Royal Society of London. Series B, Biological sciences 2014 Nachury MV
• The oral-facial-digital syndrome gene C2CD3 encodes a positive regulator of centriole elongation.
  Nature genetics 2014 Thauvin-Robinet C, Lee JS, Lopez E, Herranz-Pérez V, Shida T, Franco B, Jego L, Ye F, Pasquier L, Loget P, Gigot N, Aral B, Lopes CA, St-Onge J, Bruel AL, Thevenon J, González-Granero S, Alby C, …
• Α-tubulin K40 acetylation is required for contact inhibition of proliferation and cell-substrate adhesion.
  Molecular biology of the cell 2014 Aguilar A, Becker L, Tedeschi T, Heller S, Iomini C, Nachury MV
• Movement of Signaling Receptors Inside Primary Cilia.
  Biophysical Journal 2014 Maxence Nachury
• Effects of tubulin acetylation and tubulin acetyltransferase binding on microtubule structure.
  Molecular biology of the cell 2013 Howes SC, Alushin GM, Shida T, Nachury MV, Nogales E
• Cilia grow by taking a bite out of the cell.
  Developmental cell 2013 Pierce NW, Nachury MV
• An in vitro assay for entry into cilia reveals unique properties of the soluble diffusion barrier.
  The Journal of cell biology 2013 Breslow DK, Koslover EF, Seydel F, Spakowitz AJ, Nachury MV
• αTAT1 catalyses microtubule acetylation at clathrin-coated pits.
  Nature 2013 Montagnac G, Meas-Yedid V, Irondelle M, Castro-Castro A, Franco M, Shida T, Nachury MV, Benmerah A, Olivo-Marin JC, Chavrier P
• Exome sequencing of Bardet-Biedl syndrome patient identifies a null mutation in the BBSome subunit BBIP1 (BBS18).
  Journal of medical genetics 2013 Scheidecker S, Etard C, Pierce NW, Geoffroy V, Schaefer E, Muller J, Chennen K, Flori E, Pelletier V, Poch O, Marion V, Stoetzel C, Strähle U, Nachury MV, Dollfus H
• Single molecule imaging reveals a major role for diffusion in the exploration of ciliary space by signaling receptors.
  eLife 2013 Ye F, Breslow DK, Koslover EF, Spakowitz AJ, Nelson WJ, Nachury MV
• Structure of the α-tubulin acetyltransferase, αTAT1, and implications for tubulin-specific acetylation.
  Proceedings of the National Academy of Sciences of the United States of America 2012 Friedmann DR, Aguilar A, Fan J, Nachury MV, Marmorstein R
• A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened.
  PLoS genetics 2011 Seo S, Zhang Q, Bugge K, Breslow DK, Searby CC, Nachury MV, Sheffield VC
• Give chance a chance.
  Molecular biology of the cell 2011 Nachury MV
• Primary cilia: how to keep the riff-raff in the plasma membrane.
  Current biology : CB 2011 Breslow DK, Nachury MV
• Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome.
  Proceedings of the National Academy of Sciences of the United States of America 2011 Westlake CJ, Baye LM, Nachury MV, Wright KJ, Ervin KE, Phu L, Chalouni C, Beck JS, Kirkpatrick DS, Slusarski DC, Sheffield VC, Scheller RH, Jackson PK
• The major alpha-tubulin K40 acetyltransferase alphaTAT1 promotes rapid ciliogenesis and efficient mechanosensation.
  Proceedings of the National Academy of Sciences of the United States of America 2010 Shida T, Cueva JG, Xu Z, Goodman MB, Nachury MV
• Maxence Nachury: a transporting view of the primary cilium. Interview by Ben Short.
  The Journal of cell biology 2010 Nachury M
• A septin diffusion barrier at the base of the primary cilium maintains ciliary membrane protein distribution.
  Science (New York, N.Y.) 2010 Hu Q, Milenkovic L, Jin H, Scott MP, Nachury MV, Spiliotis ET, Nelson WJ
• The perennial organelle: assembly and disassembly of the primary cilium.
  Journal of cell science 2010 Seeley ES, Nachury MV
• Trafficking to the ciliary membrane: how to get across the periciliary diffusion barrier?
  Annual review of cell and developmental biology 2010 Nachury MV, Seeley ES, Jin H
• Constructing and deconstructing roles for the primary cilium in tissue architecture and cancer.
  Methods in cell biology 2009 Seeley ES, Nachury MV
• The BBSome.
  Current biology : CB 2009 Jin H, Nachury MV
• A BBSome subunit links ciliogenesis, microtubule stability, and acetylation.
  Developmental cell 2008 Loktev AV, Zhang Q, Beck JS, Searby CC, Scheetz TE, Bazan JF, Slusarski DC, Sheffield VC, Jackson PK, Nachury MV
• How to build a signalling organelle: A molecular pathway for biogenesis of the ciliary membrane.
  The FASEB Journal 2008 Maxence Nachury
• The nucleolar phosphatase Cdc14B is dispensable for chromosome segregation and mitotic exit in human cells.
  Cell cycle (Georgetown, Tex.) 2008 Berdougo E, Nachury MV, Jackson PK, Jallepalli PV
• Tandem affinity purification of the BBSome, a critical regulator of Rab8 in ciliogenesis.
  Methods in enzymology 2008 Nachury MV
• The END network couples spindle pole assembly to inhibition of the anaphase-promoting complex/cyclosome in early mitosis.
  Developmental cell 2007 Ban KH, Torres JZ, Miller JJ, Mikhailov A, Nachury MV, Tung JJ, Rieder CL, Jackson PK
• A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis.
  Cell 2007 Nachury MV, Loktev AV, Zhang Q, Westlake CJ, Peränen J, Merdes A, Slusarski DC, Scheller RH, Bazan JF, Sheffield VC, Jackson PK
• Emi1 stably binds and inhibits the anaphase-promoting complex/cyclosome as a pseudosubstrate inhibitor.
  Genes & development 2006 Miller JJ, Summers MK, Hansen DV, Nachury MV, Lehman NL, Loktev A, Jackson PK
• A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly.
  Cell 2005 Blower MD, Nachury M, Heald R, Weis K
• Xenopus Cdc14 alpha/beta are localized to the nucleolus and centrosome and are required for embryonic cell division.
  BMC cell biology 2004 Kaiser BK, Nachury MV, Gardner BE, Jackson PK
• Importin beta is a mitotic target of the small GTPase Ran in spindle assembly.
  Cell 2001 Nachury MV, Maresca TJ, Salmon WC, Waterman-Storer CM, Heald R, Weis K
• The direction of transport through the nuclear pore can be inverted.
  Proceedings of the National Academy of Sciences of the United States of America 1999 Nachury MV, Weis K
• Cloning and characterization of hSRP1 gamma, a tissue-specific nuclear transport factor.
  Proceedings of the National Academy of Sciences of the United States of America 1998 Nachury MV, Ryder UW, Lamond AI, Weis K