Martin Kampmann, PhD
Professor
Biochemistry and Biophysics
School of Medicine

We have pioneered a CRISPR-based functional genomics platform in human iPSC-derived neurons, glia and 3D assembloids, which enables genome-wide modifier screens of disease-relevant cell biology in patient-derived cells. We have also developed cell-type specific CRISPR screening approaches for mouse models of disease.

We use biochemistry, biophysics and cell biology to test mechanistic hypotheses generated by our functional genomics platform.

Major research questions are:

What controls protein aggregation in neurons, and why is it toxic? Why are some neuronal subtypes selectively vulnerable? Our main focus here is on the protein tau. How do non-neuronal cells such as microglia and astrocytes contribute to disease? What controls their beneficial and toxic states and functions in disease?

• Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes.
  Nature biomedical engineering 2024 Yin JA, Frick L, Scheidmann MC, Liu T, Trevisan C, Dhingra A, Spinelli A, Wu Y, Yao L, Vena DL, Knapp B, Guo J, De Cecco E, Ging K, Armani A, Oakeley EJ, Nigsch F, Jenzer J, Haegele J, Pikusa M, Täger…
• Implementation and validation of single-cell genomics experiments in neuroscience.
  Nature neuroscience 2024 Colonna M, Konopka G, Liddelow SA, Nowakowski T, Awatramani R, Bateup HS, Cadwell CR, Caglayan E, Chen JL, Gillis J, Kampmann M, Krienen F, Marsh SE, Monje M, O'Dea MR, Patani R, Pollen AA, Quintana …
• CRISPRi-based screens in iAssembloids to elucidate neuron-glia interactions.
  bioRxiv : the preprint server for biology 2024 Li E, Benitez C, Boggess SC, Koontz M, Rose IVL, Martinez D, Draeger N, Teter OM, Samelson AJ, Pierce N, Ullian EM, Kampmann M
• CRISPRi-based screen of Autism Spectrum Disorder risk genes in microglia uncovers roles of ADNP in microglia endocytosis and synaptic pruning.
  bioRxiv : the preprint server for biology 2024 Teter OM, McQuade A, Hagan V, Liang W, Dräger NM, Sattler SM, Holmes BB, Castillo VC, Papakis V, Leng K, Boggess S, Nowakowski TJ, Wells J, Kampmann M
• CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis.
  bioRxiv : the preprint server for biology 2024 Samelson AJ, Ariqat N, McKetney J, Rohanitazangi G, Bravo CP, Bose R, Travaglini KJ, Lam VL, Goodness D, Dixon G, Marzette E, Jin J, Tian R, Tse E, Abskharon R, Pan H, Carroll EC, Lawrence RE, …
• CRISPR screening by AAV episome-sequencing (CrAAVe-seq) is a highly scalable cell type-specific in vivo screening platform.
  bioRxiv : the preprint server for biology 2024 Ramani B, Rose IVL, Teyssier N, Pan A, Danner-Bocks S, Sanghal T, Yadanar L, Tian R, Ma K, Palop JJ, Kampmann M
• pyPAGE: A framework for Addressing biases in gene-set enrichment analysis-A case study on Alzheimer's disease.
  PLoS computational biology 2024 Bakulin A, Teyssier NB, Kampmann M, Khoroshkin M, Goodarzi H
• An engineered trafficking biosensor reveals a role for DNAJC13 in DOR downregulation.
  Nature chemical biology 2024 Novy B, Dagunts A, Weishaar T, Holland EE, Adoff H, Hutchinson E, De Maria M, Kampmann M, Tsvetanova NG, Lobingier BT
• mTOR activation induces endolysosomal remodeling and nonclassical secretion of IL-32 via exosomes in inflammatory reactive astrocytes.
  Journal of neuroinflammation 2024 Leng K, Rooney B, McCarthy F, Xia W, Rose IVL, Bax S, Chin M, Fathi S, Herrington KA, Leonetti M, Kao A, Fancy SPJ, Elias JE, Kampmann M
• The Neurolipid Atlas: a lipidomics resource for neurodegenerative diseases uncovers cholesterol as a regulator of astrocyte reactivity impaired by ApoE4.
  bioRxiv : the preprint server for biology 2024 Feringa FM, Hertog SJK, Wang L, Derks RJE, Kruijff I, Erlebach L, Heijneman J, Miramontes R, Pömpner N, Blomberg N, Olivier-Jimenez D, Johansen LE, Cammack AJ, Giblin A, Toomey CE, Rose IVL, Yuan H, …
• Neuronal DNA repair reveals strategies to influence CRISPR editing outcomes.
  bioRxiv : the preprint server for biology 2024 Ramadoss GN, Namaganda SJ, Hamilton JR, Sharma R, Chow KG, Macklin BL, Sun M, Liu JC, Fellmann C, Watry HL, Jin J, Perez BS, Sandoval Espinoza CR, Matia MP, Lu SH, Judge LM, Nussenzweig A, Adamson B, …
• The disease-causing tau V337M mutation induces tau hypophosphorylation and perturbs axon morphology pathways.
  bioRxiv : the preprint server for biology 2024 Mohl GA, Dixon G, Marzette E, McKetney J, Samelson AJ, Serras CP, Jin J, Li A, Boggess SC, Swaney DL, Kampmann M
• Functional multi-omics reveals genetic and pharmacologic regulation of surface CD38 in multiple myeloma.
  2024 Priya Choudhry, Corynn Kasap, Bonell Patiño-Escobar, Olivia Gugliemini, Huimin Geng, Vishesh Sarin, Amrik Kang, Audrey Kishishita, Sham Rampersaud, Letitia Sarah, Yu-Hsiu T. Lin, Neha Paranjape, …
• Tau fibrils induce nanoscale membrane damage and nucleate cytosolic tau at lysosomes.
  Proceedings of the National Academy of Sciences of the United States of America 2024 Rose K, Jepson T, Shukla S, Maya-Romero A, Kampmann M, Xu K, Hurley JH
• B Cell-mediated Neurotoxicity in Multiple Sclerosis (P7-6.003).
  Neurology 2024 Chaitrali Saha, Amy Zhang, Kun Leng, Chloe Gerungan, Ahmed Abdelhak, Refujia Gomez, Meagan Harms, Asritha Tubati, Tiffany Cooper, Martineau Louine, Joseph Sabatino, Martin Kampmann, Ari Green, Jorge …
• Human iPSC 4R tauopathy model uncovers modifiers of tau propagation.
  Cell 2024 Parra Bravo C, Giani AM, Madero-Perez J, Zhao Z, Wan Y, Samelson AJ, Wong MY, Evangelisti A, Cordes E, Fan L, Ye P, Zhu D, Pozner T, Mercedes M, Patel T, Yarahmady A, Carling GK, Sterky FH, Lee VMY, …
• Molecular and cellular mechanisms of selective vulnerability in neurodegenerative diseases.
  Nature reviews. Neuroscience 2024 Kampmann M
• International consensus guidelines for the definition, detection, and interpretation of autophagy-dependent ferroptosis.
  Autophagy 2024 Chen X, Tsvetkov AS, Shen HM, Isidoro C, Ktistakis NT, Linkermann A, Koopman WJH, Simon HU, Galluzzi L, Luo S, Xu D, Gu W, Peulen O, Cai Q, Rubinsztein DC, Chi JT, Zhang DD, Li C, Toyokuni S, Liu J, …
• Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesis.
  bioRxiv : the preprint server for biology 2024 Sun N, Teyssier N, Wang B, Drake S, Seyler M, Zaltsman Y, Everitt A, Teerikorpi N, Willsey HR, Goodarzi H, Tian R, Kampmann M, Willsey AJ
• Alzheimer’s disease risk genes modulate interferon-responsive microglia.
  Alzheimer's & Dementia 2023 Amanda McQuade, Vincent Cele Castillo, Nina Drager, Martin Kampmann
• Multiomics of iPSC-derived ApoE3- and ApoE4 astrocytes and microglia uncovers cell type specific dysregulation of immunometabolism.
  Alzheimer's & Dementia 2023 Rik van der Kant, Niek Blomberg, Lena Erlebach, Femke M Feringa, Jorin Heijneman, Sascha Koppes, Iris Daphne Kruijff, Amanda McQuade, Ricardo Miramontes, Damien Olivier-Jimenez, Aiko Robert, Leslie M.…
• Phospholipase D3 drives microglial response to amyloid pathology in Alzheimer’s disease.
  Alzheimer's & Dementia 2023 Matthew J Rosene, Emma Danhash, Shih-Feng You, Abhirami Kannan Iyer, Simon Hsu, Mariana Acquarone de Sa Lopes, Logan Brase, Anthony Verbeck, Rita Martinez, Clare E Wallace, Zeran Li, Ping Yan, Nina …
• Toward a CRISPR understanding of gene function in human brain development.
  Cell stem cell 2023 Li E, Kampmann M
• Pharmacologic activation of a compensatory integrated stress response kinase promotes mitochondrial remodeling in PERK-deficient cells.
  Cell chemical biology 2023 Perea V, Baron KR, Dolina V, Aviles G, Kim G, Rosarda JD, Guo X, Kampmann M, Wiseman RL
• Neurodegeneration enters the era of functional genomics.
  Science (New York, N.Y.) 2023 Aguzzi A, Kampmann M
• Tau fibrils induce nanoscale membrane damage and nucleate cytosolic tau at lysosomes.
  bioRxiv : the preprint server for biology 2023 Rose K, Jepson T, Shukla S, Maya-Romero A, Kampmann M, Xu K, Hurley JH
• DELE1 oligomerization promotes integrated stress response activation.
  Nature structural & molecular biology 2023 Yang J, Baron KR, Pride DE, Schneemann A, Guo X, Chen W, Song AS, Aviles G, Kampmann M, Wiseman RL, Lander GC
• Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid droplet regulation.
  Developmental cell 2023 Roberts MA, Deol KK, Mathiowetz AJ, Lange M, Leto DE, Stevenson J, Hashemi SH, Morgens DW, Easter E, Heydari K, Nalls MA, Bassik MC, Kampmann M, Kopito RR, Faghri F, Olzmann JA
• Human iPSC 4R tauopathy model uncovers modifiers of tau propagation.
  bioRxiv : the preprint server for biology 2023 Bravo CP, Giani AM, Perez JM, Zhao Z, Samelson A, Wong MY, Evangelisti A, Fan L, Pozner T, Mercedes M, Ye P, Patel T, Yarahmady A, Carling G, Lee VMY, Sharma M, Mok SA, Luo W, Zhao M, Kampmann M, Gong…
• Pharmacologic Activation of a Compensatory Integrated Stress Response Kinase Promotes Mitochondrial Remodeling in PERK-deficient Cells.
  bioRxiv : the preprint server for biology 2023 Perea V, Baron KR, Dolina V, Aviles G, Rosarda JD, Guo X, Kampmann M, Wiseman RL
• Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles.
  Nature communications 2023 Lan Y, van Leur SW, Fernando JA, Wong HH, Kampmann M, Siu L, Zhang J, Li M, Nicholls JM, Sanyal S
• Neurons require glucose uptake and glycolysis in vivo.
  Cell reports 2023 Li H, Guglielmetti C, Sei YJ, Zilberter M, Le Page LM, Shields L, Yang J, Nguyen K, Tiret B, Gao X, Bennett N, Lo I, Dayton TL, Kampmann M, Huang Y, Rathmell JC, Vander Heiden M, Chaumeil MM, Nakamura…
• A molecular glue approach to control the half-life of CRISPR-based technologies.
  bioRxiv : the preprint server for biology 2023 Sreekanth V, Jan M, Zhao KT, Lim D, Davis JR, McConkey M, Kovalcik V, Barkal S, Law BK, Fife J, Tian R, Vinyard ME, Becerra B, Kampmann M, Sherwood RI, Pinello L, Liu DR, Ebert BL, Choudhary A
• Granulin loss of function in human mature brain organoids implicates astrocytes in TDP-43 pathology.
  Stem cell reports 2023 de Majo M, Koontz M, Marsan E, Salinas N, Ramsey A, Kuo YM, Seo K, Li H, Dräger N, Leng K, Gonzales SL, Kurnellas M, Miyaoka Y, Klim JR, Kampmann M, Ward ME, Huang EJ, Ullian EM
• Genomic profiling of HIV-1 integration in microglia cells links viral integration to the topologically associated domains.
  Cell reports 2023 Rheinberger M, Costa AL, Kampmann M, Glavas D, Shytaj IL, Sreeram S, Penzo C, Tibroni N, Garcia-Mesa Y, Leskov K, Fackler OT, Vlahovicek K, Karn J, Lucic B, Herrmann C, Lusic M
• MS4A4A modifies the risk of Alzheimer disease by regulating lipid metabolism and immune response in a unique microglia state.
  medRxiv : the preprint server for health sciences 2023 You SF, Brase L, Filipello F, Iyer AK, Del-Aguila J, He J, D'Oliveira Albanus R, Budde J, Norton J, Gentsch J, Dräger NM, Sattler SM, Kampmann M, Piccio L, Morris JC, Perrin RJ, McDade E, Dominantly …
• An E3 ligase network engages GCN1 to promote the degradation of translation factors on stalled ribosomes.
  Cell 2023 Oltion K, Carelli JD, Yang T, See SK, Wang HY, Kampmann M, Taunton J
• Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors.
  eLife 2022 Replogle JM, Bonnar JL, Pogson AN, Liem CR, Maier NK, Ding Y, Russell BJ, Wang X, Leng K, Guna A, Norman TM, Pak RA, Ramos DM, Ward ME, Gilbert LA, Kampmann M, Weissman JS, Jost M
• Pathological Validation of RORB and CDH9 as Selectively Vulnerable Neurons in Alzheimer’s Disease.
  Alzheimer's & Dementia 2022 Rakin T Nasar, Felipe Luiz Pereira, Song Hua Li, Silvia Miramontes, Martin Kampmann, Kun Leng, William W. Seeley, Salvatore Spina, Bruce L. Miller, Lea Tenenholz Grinberg
• A reference human induced pluripotent stem cell line for large-scale collaborative studies.
  Cell stem cell 2022 Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, Oguro H, Kanaujiya J, Zou J, Sebesta D, Pratt G, Cross E, Blockwick J, Buxton P, Kinner-Bibeau L, Medura C, Tompkins C, Hughes S, …
• Towards elucidating disease-relevant states of neurons and glia by CRISPR-based functional genomics.
  Genome medicine 2022 Leng K, Kampmann M
• Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin.
  Nature communications 2022 Kim H, Leng K, Park J, Sorets AG, Kim S, Shostak A, Embalabala RJ, Mlouk K, Katdare KA, Rose IVL, Sturgeon SM, Neal EH, Ao Y, Wang S, Sofroniew MV, Brunger JM, McMahon DG, Schrag MS, Kampmann M, …
• CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states.
  Nature neuroscience 2022 Leng K, Rose IVL, Kim H, Xia W, Romero-Fernandez W, Rooney B, Koontz M, Li E, Ao Y, Wang S, Krawczyk M, Tcw J, Goate A, Zhang Y, Ullian EM, Sofroniew MV, Fancy SPJ, Schrag MS, Lippmann ES, Kampmann M
• Reduced endosomal microautophagy activity in aging associates with enhanced exocyst-mediated protein secretion.
  Aging cell 2022 Krause GJ, Diaz A, Jafari M, Khawaja RR, Agullo-Pascual E, Santiago-Fernández O, Richards AL, Chen KH, Dmitriev P, Sun Y, See SK, Abdelmohsen K, Mazan-Mamczarz K, Krogan NJ, Gorospe M, Swaney DL, …
• Functional regulatory variants implicate distinct transcriptional networks in dementia.
  Science (New York, N.Y.) 2022 Cooper YA, Teyssier N, Dräger NM, Guo Q, Davis JE, Sattler SM, Yang Z, Patel A, Wu S, Kosuri S, Coppola G, Kampmann M, Geschwind DH
• A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states.
  Nature neuroscience 2022 Dräger NM, Sattler SM, Huang CT, Teter OM, Leng K, Hashemi SH, Hong J, Aviles G, Clelland CD, Zhan L, Udeochu JC, Kodama L, Singleton AB, Nalls MA, Ichida J, Ward ME, Faghri F, Gan L, Kampmann M
• Phenotypic Screening Using High-Content Imaging to Identify Lysosomal pH Modulators in a Neuronal Cell Model.
  ACS chemical neuroscience 2022 Chin MY, Ang KH, Davies J, Alquezar C, Garda VG, Rooney B, Leng K, Kampmann M, Arkin MR, Kao AW
• Conversations with LGBT+ scientists about visibility, leadership and climbing the career ladder.
  Journal of cell science 2022 Bristow RG, Engel J, Jayasinghe I, Kampmann M, James Sansom O, Bryant DM
• Selective vulnerabilities in the proteostasis network of castration-resistant prostate cancer.
  Cell chemical biology 2022 Shkedi A, Taylor IR, Echtenkamp F, Ramkumar P, Alshalalfa M, Rivera-Márquez GM, Moses MA, Shao H, Karnes RJ, Neckers L, Feng F, Kampmann M, Gestwicki JE
• NudC guides client transfer between the Hsp40/70 and Hsp90 chaperone systems.
  Molecular cell 2022 Biebl MM, Delhommel F, Faust O, Zak KM, Agam G, Guo X, Mühlhofer M, Dahiya V, Hillebrand D, Popowicz GM, Kampmann M, Lamb DC, Rosenzweig R, Sattler M, Buchner J
• BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2.
  Nature cell biology 2022 Samelson AJ, Tran QD, Robinot R, Carrau L, Rezelj VV, Kain AM, Chen M, Ramadoss GN, Guo X, Lim SA, Lui I, Nuñez JK, Rockwood SJ, Wang J, Liu N, Carlson-Stevermer J, Oki J, Maures T, Holden K, Weissman…
• CRISPR-Based Screening for Stress Response Factors in Mammalian Cells.
  Methods in molecular biology (Clifton, N.J.) 2022 Guo X, Kampmann M
• Evaluation of a nasopharyngeal stent in patients with obstructive sleep-related breathing disorders.
  The Journal of international medical research 2022 Dellweg A, Kampmann M, Tschopp K
• The Lonafarnib target, Rhes, is uniquely dysregulated in tauopathies: A human postmortem study.
  Alzheimer's & Dementia 2021 Alexander J. Ehrenberg, Kun Leng, Kaitlin Letourneau, Israel Hernandez, Caroline Lew, William W. Seeley, Salvatore Spina, Bruce L. Miller, Helmut Heinsen, Martin Kampmann, Kenneth S. Kosik, Lea T. …
• Functional Multi-Omics Reveals Genetic and Pharmacologic Regulation of Surface CD38 in Multiple Myeloma.
  Blood 2021 Priya Choudhry, Olivia Gugliemini, Huimin Geng, Vishesh Sarin, Letitia Sarah, Yu-Hsiu T. Lin, Neha Paranjape, Poornima Ramkumar, Makeba Marcoulis, Donghui Wang, Paul Phojanakong, Veronica Steri, Byron…
• BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2.
  bioRxiv : the preprint server for biology 2021 Samelson AJ, Tran QD, Robinot R, Carrau L, Rezelj VV, Mac Kain A, Chen M, Ramadoss GN, Guo X, Lim SA, Lui I, Nunez J, Rockwood SJ, Wang J, Liu N, Carlson-Stevermer J, Oki J, Maures T, Holden K, …
• Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.
  eLife 2021 Le Vasseur M, Friedman J, Jost M, Xu J, Yamada J, Kampmann M, Horlbeck MA, Salemi MR, Phinney BS, Weissman JS, Nunnari J
• Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis.
  Nature neuroscience 2021 Tian R, Abarientos A, Hong J, Hashemi SH, Yan R, Dräger N, Leng K, Nalls MA, Singleton AB, Xu K, Faghri F, Kampmann M
• A high-throughput CRISPR interference screen for dissecting functional regulators of GPCR/cAMP signaling.
  The FASEB Journal 2021 Khairunnisa Semesta, Ruilin Tian, Martin Kampmann, Mark Zastrow, Nikoleta Tsvetanova
• Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing.
  Cell 2021 Nuñez JK, Chen J, Pommier GC, Cogan JZ, Replogle JM, Adriaens C, Ramadoss GN, Shi Q, Hung KL, Samelson AJ, Pogson AN, Kim JYS, Chung A, Leonetti MD, Chang HY, Kampmann M, Bernstein BE, Hovestadt V, …
• Patterns of neuronal Rhes as a novel hallmark of tauopathies.
  Acta neuropathologica 2021 Ehrenberg AJ, Leng K, Letourneau KN, Hernandez I, Lew C, Seeley WW, Spina S, Miller B, Heinsen H, Kampmann M, Kosik KS, Grinberg LT
• Functional genomics screen identifies proteostasis targets that modulate prion protein (PrP) stability.
  Cell stress & chaperones 2021 Abrams J, Arhar T, Mok SA, Taylor IR, Kampmann M, Gestwicki JE
• Image-based pooled whole-genome CRISPRi screening for subcellular phenotypes.
  The Journal of cell biology 2021 Kanfer G, Sarraf SA, Maman Y, Baldwin H, Dominguez-Martin E, Johnson KR, Ward ME, Kampmann M, Lippincott-Schwartz J, Youle RJ
• A high-throughput CRISPR interference screen for dissecting functional regulators of GPCR/cAMP signaling.
  PLoS genetics 2020 Semesta KM, Tian R, Kampmann M, von Zastrow M, Tsvetanova NG
• Defining the ATPome reveals cross-optimization of metabolic pathways.
  Nature communications 2020 Bennett NK, Nguyen MK, Darch MA, Nakaoka HJ, Cousineau D, Ten Hoeve J, Graeber TG, Schuelke M, Maltepe E, Kampmann M, Mendelsohn BA, Nakamura JL, Nakamura K
• Robust Sequence Determinants of α-Synuclein Toxicity in Yeast Implicate Membrane Binding.
  ACS chemical biology 2020 Newberry RW, Arhar T, Costello J, Hartoularos GC, Maxwell AM, Naing ZZC, Pittman M, Reddy NR, Schwarz DMC, Wassarman DR, Wu TS, Barrero D, Caggiano C, Catching A, Cavazos TB, Estes LS, Faust B, Fink …
• CRISPR-based screens uncover determinants of immunotherapy response in multiple myeloma.
  Blood advances 2020 Ramkumar P, Abarientos AB, Tian R, Seyler M, Leong JT, Chen M, Choudhry P, Hechler T, Shah N, Wong SW, Martin TG, Wolf JL, Roybal KT, Pahl A, Taunton J, Wiita AP, Kampmann M
• Pharmaceutical-Grade Rigosertib Is a Microtubule-Destabilizing Agent.
  Molecular cell 2020 Jost M, Chen Y, Gilbert LA, Horlbeck MA, Krenning L, Menchon G, Rai A, Cho MY, Stern JJ, Prota AE, Kampmann M, Akhmanova A, Steinmetz MO, Tanenbaum ME, Weissman JS
• A High-throughput CRISPR Interference Screen to Dissect Functional Regulators of GPCR/cAMP Signaling.
  The FASEB Journal 2020 Khairunnisa Mentari Semesta, Ruilin Tian, Martin Kampmann, Mark von Zastrow, Nikoleta G. Tsvetanova
• LRP1 is a master regulator of tau uptake and spread.
  Nature 2020 Rauch JN, Luna G, Guzman E, Audouard M, Challis C, Sibih YE, Leshuk C, Hernandez I, Wegmann S, Hyman BT, Gradinaru V, Kampmann M, Kosik KS
• Deep mutational scanning reveals the structural basis for α-synuclein activity.
  Nature chemical biology 2020 Newberry RW, Leong JT, Chow ED, Kampmann M, DeGrado WF
• Gene expression and cell identity controlled by anaphase-promoting complex.
  Nature 2020 Oh E, Mark KG, Mocciaro A, Watson ER, Prabu JR, Cha DD, Kampmann M, Gamarra N, Zhou CY, Rape M
• Compromised function of the ESCRT pathway promotes endolysosomal escape of tau seeds and propagation of tau aggregation.
  The Journal of biological chemistry 2019 Chen JJ, Nathaniel DL, Raghavan P, Nelson M, Tian R, Tse E, Hong JY, See SK, Mok SA, Hein MY, Southworth DR, Grinberg LT, Gestwicki JE, Leonetti MD, Kampmann M
• Quantitative Proteomics of Uukuniemi Virus-host Cell Interactions Reveals GBF1 as Proviral Host Factor for Phleboviruses.
  Molecular & cellular proteomics : MCP 2019 Uckeley ZM, Moeller R, Kühn LI, Nilsson E, Robens C, Lasswitz L, Lindqvist R, Lenman A, Passos V, Voss Y, Sommerauer C, Kampmann M, Goffinet C, Meissner F, Överby AK, Lozach PY, Gerold G
• Ceapins block the unfolded protein response sensor ATF6α by inducing a neomorphic inter-organelle tether.
  eLife 2019 Torres SE, Gallagher CM, Plate L, Gupta M, Liem CR, Guo X, Tian R, Stroud RM, Kampmann M, Weissman JS, Walter P
• Probing the Global Cellular Responses to Lipotoxicity Caused by Saturated Fatty Acids.
  Molecular cell 2019 Piccolis M, Bond LM, Kampmann M, Pulimeno P, Chitraju C, Jayson CBK, Vaites LP, Boland S, Lai ZW, Gabriel KR, Elliott SD, Paulo JA, Harper JW, Weissman JS, Walther TC, Farese RV
• Heterochromatin anomalies and double-stranded RNA accumulation underlie C9orf72 poly(PR) toxicity.
  Science (New York, N.Y.) 2019 Zhang YJ, Guo L, Gonzales PK, Gendron TF, Wu Y, Jansen-West K, O'Raw AD, Pickles SR, Prudencio M, Carlomagno Y, Gachechiladze MA, Ludwig C, Tian R, Chew J, DeTure M, Lin WL, Tong J, Daughrity LM, Yue …
• Relapse-associated AURKB blunts the glucocorticoid sensitivity of B cell acute lymphoblastic leukemia.
  Proceedings of the National Academy of Sciences of the United States of America 2019 Poulard C, Kim HN, Fang M, Kruth K, Gagnieux C, Gerke DS, Bhojwani D, Kim YM, Kampmann M, Stallcup MR, Pufall MA
• Identifying Factors in Multiple Myeloma Controlling Response to B-Cell Maturation Antigen (BCMA)-Targeted Immunotherapy Using CRISPR-Based Functional Genomics.
  Blood 2018 Poornima Ramkumar, Jaime Leong, Meghan Seyler, Stratton J Georgoulis, Axel Hyrenius Wittsten, Priya Choudhry, Kole Roybal, Arun P Wiita, Martin Kampmann
• A high-throughput screen of real-time ATP levels in individual cells reveals mechanisms of energy failure.
  PLoS biology 2018 Mendelsohn BA, Bennett NK, Darch MA, Yu K, Nguyen MK, Pucciarelli D, Nelson M, Horlbeck MA, Gilbert LA, Hyun W, Kampmann M, Nakamura JL, Nakamura K
• The Psychiatric Cell Map Initiative: A Convergent Systems Biological Approach to Illuminating Key Molecular Pathways in Neuropsychiatric Disorders.
  Cell 2018 Willsey AJ, Morris MT, Wang S, Willsey HR, Sun N, Teerikorpi N, Baum TB, Cagney G, Bender KJ, Desai TA, Srivastava D, Davis GW, Doudna J, Chang E, Sohal V, Lowenstein DH, Li H, Agard D, Keiser MJ, …
• Extending chemical perturbations of the ubiquitin fitness landscape in a classroom setting reveals new constraints on sequence tolerance.
  Biology open 2018 Mavor D, Barlow KA, Asarnow D, Birman Y, Britain D, Chen W, Green EM, Kenner LR, Mensa B, Morinishi LS, Nelson CA, Poss EM, Suresh P, Tian R, Arhar T, Ary BE, Bauer DP, Bergman ID, Brunetti RM, Chio …
• Mapping the Genetic Landscape of Human Cells.
  Cell 2018 Horlbeck MA, Xu A, Wang M, Bennett NK, Park CY, Bogdanoff D, Adamson B, Chow ED, Kampmann M, Peterson TR, Nakamura K, Fischbach MA, Weissman JS, Gilbert LA
• Tau Internalization is Regulated by 6-O Sulfation on Heparan Sulfate Proteoglycans (HSPGs).
  Scientific reports 2018 Rauch JN, Chen JJ, Sorum AW, Miller GM, Sharf T, See SK, Hsieh-Wilson LC, Kampmann M, Kosik KS
• CRISPR-based genetic interaction maps inform therapeutic strategies in cancer.
  Translational cancer research 2018 Ramkumar P, Kampmann M
• Dual gene activation and knockout screen reveals directional dependencies in genetic networks.
  Nature biotechnology 2018 Boettcher M, Tian R, Blau JA, Markegard E, Wagner RT, Wu D, Mo X, Biton A, Zaitlen N, Fu H, McCormick F, Kampmann M, McManus MT
• CRISPRi and CRISPRa Screens in Mammalian Cells for Precision Biology and Medicine.
  ACS chemical biology 2017 Kampmann M
• Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent.
  Molecular cell 2017 Jost M, Chen Y, Gilbert LA, Horlbeck MA, Krenning L, Menchon G, Rai A, Cho MY, Stern JJ, Prota AE, Kampmann M, Akhmanova A, Steinmetz MO, Tanenbaum ME, Weissman JS
• Abstract A30: Identifying microenvironment-dependent vulnerabilities in multiple myeloma using CRISPRi.
  Finding Synthetic Lethal Interactions through Functional Genomics 2017 Poornima Ramkumar, Jaime Tawney, Diego Acosta-Alvear, Martin Kampmann
• CRISPulator: a discrete simulation tool for pooled genetic screens.
  BMC bioinformatics 2017 Nagy T, Kampmann M
• Elucidating drug targets and mechanisms of action by genetic screens in mammalian cells.
  Chemical communications (Cambridge, England) 2017 Kampmann M
• A CRISPR Approach to Neurodegenerative Diseases.
  Trends in molecular medicine 2017 Kampmann M
• Suppression of B-cell development genes is key to glucocorticoid efficacy in treatment of acute lymphoblastic leukemia.
  Blood 2017 Kruth KA, Fang M, Shelton DN, Abu-Halawa O, Mahling R, Yang H, Weissman JS, Loh ML, Müschen M, Tasian SK, Bassik MC, Kampmann M, Pufall MA
• Inhibiting pathways involved in B-cell development enhances sensitivity of B-cell acute lymphoblastic leukemia to glucocorticoids.
  The FASEB Journal 2017 Miles A Pufall, Karina Kruth, Mimi A Fang, Dawne Shelton, Ossama Abu-Halawa, Sarah K Tasian, Martin Kampmann
• The mTOR Complex Controls HIV Latency.
  Cell host & microbe 2016 Besnard E, Hakre S, Kampmann M, Lim HW, Hosmane NN, Martin A, Bassik MC, Verschueren E, Battivelli E, Chan J, Svensson JP, Gramatica A, Conrad RJ, Ott M, Greene WC, Krogan NJ, Siliciano RF, Weissman …
• Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation.
  eLife 2016 Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS
• Parallel shRNA and CRISPR-Cas9 screens enable antiviral drug target identification.
  Nature chemical biology 2016 Deans RM, Morgens DW, Ökesli A, Pillay S, Horlbeck MA, Kampmann M, Gilbert LA, Li A, Mateo R, Smith M, Glenn JS, Carette JE, Khosla C, Bassik MC
• Abstract A2-12: Opposing roles of the 19S regulatory and 20S core proteasomal subunits in controlling sensitivity to proteasome inhibitors.
  Genomics and Target Discovery 2015 Min Y. Cho, Diego Acosta-Alvear, Martin Kampmann, Thomas Wild, Chuna Ram Choudhary, Jonathan S. Weissman, Peter Walter
• Abstract A2-56: Cancer-specific synthetic lethality and network rewiring elucidated by genetic interaction maps and CRISPR-based gain- and loss-of-function screens.
  Network-Based Cancer Biology 2015 Martin Kampmann, Diego Acosta-Alvear, Luke A. Gilbert, Max A. Horlbeck, Min Y. Cho, Britt Adamson, Jacqueline Villalta, Yuwen Chen, Peter Walter, Jonathan S. Weissman
• Abstract A2-57: Identification of genetic vulnerabilities within the proteostasis network of multiple myeloma.
  Network-Based Cancer Biology 2015 Diego Acosta-Alvear, Martin Kampmann, Min Y. Cho, Blake T. Aftab, Xiaokai Li, Jason E. Gestwicki, Marc A. Shuman, Jonathan S. Weissman, Peter Walter
• Abstract PR05: Using genome-scale CRISPR-mediated loss-of-function and gain-of-function screens to analyze gene-drug interactions in cancer.
  Genomics and Target Discovery 2015 Luke A. Gilbert, Marvin E. Tanenbaum, Max A. Horlbeck, Britt Adamson, Jacqueline E. Villalta, Ronald D. Vale, Martin Kampmann, Jonathan S. Weissman
• Targeting the AAA ATPase p97 as an Approach to Treat Cancer through Disruption of Protein Homeostasis.
  Cancer cell 2015 Anderson DJ, Le Moigne R, Djakovic S, Kumar B, Rice J, Wong S, Wang J, Yao B, Valle E, Kiss von Soly S, Madriaga A, Soriano F, Menon MK, Wu ZY, Kampmann M, Chen Y, Weissman JS, Aftab BT, Yakes FM, …
• Abstract A43: Opposing roles of the 19S regulatory- and 20S core-proteasomal subunits in controlling sensitivity of multiple myeloma cells to proteasome inhibition.
  Myeloma 2015 Diego Acosta-Alvear, Martin Kampmann, Min Y. Cho, Thomas Wild, Chunaram Choudhary, Marc A. Shuman, Jonathan S. Weissman, Peter Walter
• Paradoxical resistance of multiple myeloma to proteasome inhibitors by decreased levels of 19S proteasomal subunits.
  eLife 2015 Acosta-Alvear D, Cho MY, Wild T, Buchholz TJ, Lerner AG, Simakova O, Hahn J, Korde N, Landgren O, Maric I, Choudhary C, Walter P, Weissman JS, Kampmann M
• Next-generation libraries for robust RNA interference-based genome-wide screens.
  Proceedings of the National Academy of Sciences of the United States of America 2015 Kampmann M, Horlbeck MA, Chen Y, Tsai JC, Bassik MC, Gilbert LA, Villalta JE, Kwon SC, Chang H, Kim VN, Weissman JS
• Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.
  eLife 2015 Sidrauski C, Tsai JC, Kampmann M, Hearn BR, Vedantham P, Jaishankar P, Sokabe M, Mendez AS, Newton BW, Tang EL, Verschueren E, Johnson JR, Krogan NJ, Fraser CS, Weissman JS, Renslo AR, Walter P
• Corrigendum: functional genomics platform for pooled screening and generation of mammalian genetic interaction maps.
  Nature protocols 2015 Kampmann M, Bassik MC, Weissman JS
• Validation of the Hsp70-Bag3 protein-protein interaction as a potential therapeutic target in cancer.
  Molecular cancer therapeutics 2015 Li X, Colvin T, Rauch JN, Acosta-Alvear D, Kampmann M, Dunyak B, Hann B, Aftab BT, Murnane M, Cho M, Walter P, Weissman JS, Sherman MY, Gestwicki JE
• Weak base pairing in both seed and 3' regions reduces RNAi off-targets and enhances si/shRNA designs.
  Nucleic acids research 2014 Gu S, Zhang Y, Jin L, Huang Y, Zhang F, Bassik MC, Kampmann M, Kay MA
• Unraveling the mechanism of cell death induced by chemical fibrils.
  Nature chemical biology 2014 Julien O, Kampmann M, Bassik MC, Zorn JA, Venditto VJ, Shimbo K, Agard NJ, Shimada K, Rheingold AL, Stockwell BR, Weissman JS, Wells JA
• Functional genomics platform for pooled screening and generation of mammalian genetic interaction maps.
  Nature protocols 2014 Kampmann M, Bassik MC, Weissman JS
• Unraveling the Mechanism of Cell Death Induced by Chemical and Protein Fibrils.
  Biophysical Journal 2014 Olivier Julien, Martin Kampmann, Michael C. Bassik, Vincent J. Venditto, Julie A. Zorn, Arnold L. Rheingold, Jonathan S. Weissman, James A. Wells
• Next-Generation NAMPT Inhibitors For ALL Identified By Sequential High-Throughput Phenotypic Chemical and Functional Genomic Screens.
  Blood 2013 Michael C. Wei, Christina J. Matheny, Michael C. Bassik, Alicia J. Donnelly, Martin Kampmann, Masayuki Iwasaki, Obdulio Piloto, David E. Solow-Cordero, Donna Bouley, Rachel E. Rau, Patrick Brown, …
• Next-generation NAMPT inhibitors identified by sequential high-throughput phenotypic chemical and functional genomic screens.
  Chemistry & biology 2013 Matheny CJ, Wei MC, Bassik MC, Donnelly AJ, Kampmann M, Iwasaki M, Piloto O, Solow-Cordero DE, Bouley DM, Rau R, Brown P, McManus MT, Weissman JS, Cleary ML
• Abstract C01: Systematic blueprinting of genetic vulnerabilities using ultra-complex shRNA libraries identifies genes synergizing with proteasome inhibition or blockade of IRE1 in multiple myeloma cells.
  Signaling and Drug Resistance 2013 Diego Acosta-Alvear, Martin Kampmann, Michael C. Bassik, Crystal P. Lee, Min Y. Cho, Blake T. Aftab, Xiaokai Li, Jason E. Gestwicki, Marc A. Shuman, Jonathan S. Weissman, Peter Walter
• Abstract PR07: Systematic genetic interaction maps reveal rewiring of the stress response network and resulting vulnerabilities in leukemia and multiple myeloma cells.
  Interpreting the Cancer Genome 2013 Martin Kampmann, Diego Acosta-Alvear, Min Cho, Yuwen Chen, Xiaokai Li, Luke Gilbert, Blake T. Aftab, Jason Gestwicki, Peter Walter, Jonathan S. Weissman
• Integrated platform for genome-wide screening and construction of high-density genetic interaction maps in mammalian cells.
  Proceedings of the National Academy of Sciences of the United States of America 2013 Kampmann M, Bassik MC, Weissman JS
• Abstract A08: Systematic identification of genetic vulnerabilities synergizing with proteasome and IRE1 inhibition in multiple myeloma cells using ultra-complex shRNA libraries.
  Genome Scale Synthetic Lethal Screens in Model Organisms 2013 Diego Acosta-Alvear, Martin Kampmann, Michael C. Bassik, Crystal P. Lee, Marc A. Shuman, Jonathan S. Weissman, Peter Walter
• Abstract PR06: Systematic genetic interaction maps reveal synthetic-lethal vulnerabilities in leukemia and multiple myeloma.
  Systemic Functional Genomics 2013 Martin Kampmann, Diego Acosta-Alvear, Michael C. Bassik, Yuwen Chen, Peter Walter, Jonathan S. Weissman
• A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility.
  Cell 2013 Bassik MC, Kampmann M, Lebbink RJ, Wang S, Hein MY, Poser I, Weibezahn J, Horlbeck MA, Chen S, Mann M, Hyman AA, Leproust EM, McManus MT, Weissman JS
• Conserved spatial organization of FG domains in the nuclear pore complex.
  Biophysical journal 2013 Atkinson CE, Mattheyses AL, Kampmann M, Simon SM
• Effect of FG Motifs on Ordering Proteins within the Nuclear Pore Complex.
  Biophysical Journal 2012 Claire E. Atkinson, Alexa L. Mattheyses, Martin Kampmann, Sanford M. Simon
• Knocking out the door to tunicamycin entry.
  Proceedings of the National Academy of Sciences of the United States of America 2011 Bassik MC, Kampmann M
• Mapping the orientation of nuclear pore proteins in living cells with polarized fluorescence microscopy.
  Nature structural & molecular biology 2011 Kampmann M, Atkinson CE, Mattheyses AL, Simon SM
• Protein Domain Organization in the Nuclear Pore Complex Studied by Fluorescence Anisotropy.
  Biophysical Journal 2011 Alexa L. Mattheyses, Martin Kampmann, Claire E. Atkinson, Sanford M. Simon
• The Structure of the Nuclear Pore Complex Studied by Fluorescence Anisotropy.
  Biophysical Journal 2011 Claire E. Atkinson, Martin Kampmann, Alexa L. Mattheyses, Sanford M. Simon
• Fluorescence anisotropy reveals order and disorder of protein domains in the nuclear pore complex.
  Biophysical journal 2010 Mattheyses AL, Kampmann M, Atkinson CE, Simon SM
• Use of RNA tertiary interaction modules for the crystallisation of the spliceosomal snRNP core domain.
  Journal of molecular biology 2010 Leung AK, Kambach C, Kondo Y, Kampmann M, Jinek M, Nagai K
• Biochemistry. Nascent proteins caught in the act.
  Science (New York, N.Y.) 2009 Kampmann M, Blobel G
• Structure of a trimeric nucleoporin complex reveals alternate oligomerization states.
  Proceedings of the National Academy of Sciences of the United States of America 2009 Nagy V, Hsia KC, Debler EW, Kampmann M, Davenport AM, Blobel G, Hoelz A
• Three-dimensional structure and flexibility of a membrane-coating module of the nuclear pore complex.
  Nature structural & molecular biology 2009 Kampmann M, Blobel G
• Facilitated diffusion in chromatin lattices: mechanistic diversity and regulatory potential.
  Molecular microbiology 2005 Kampmann M
• Reverse gyrase has heat-protective DNA chaperone activity independent of supercoiling.
  Nucleic acids research 2004 Kampmann M, Stock D
• Obstacle bypass in protein motion along DNA by two-dimensional rather than one-dimensional sliding.
  The Journal of biological chemistry 2004 Kampmann M