Joanne Engel, MD, PhD
Professor
Medicine
School of Medicine
My lab is interested in the complex interplay between bacterial pathogens and host cells. In particular, we study two important human pathogens, Chlamydia trachomatis and Pseudomonas aeruginosa. Our strengths include using multidisciplinary approaches to these studies—allowing the pathogen to be our tutor.
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We have utilized bacterial genetics and genetic screens, molecular biology, cellular microbiology, host cell biology with advanced immunofluorescence microscopy, genome-wide RNAi screens, bioinformatics, and proteomics to rigorously understand the mechanisms by which they subvert host cell functions to cause disease. Seminal contributions that our group has made to the study of P. aeruginosa-host interactions is (i) the discovery of the P. aeruginosa type III secretion system and one of the secreted effectors ExoU and the demonstration that the P. aeruginosa type III secretion system is important for virulence in cell-culture, mouse, and human infections (ii) demonstrating that the type III secreted toxin ExoT inhibits wound repair through redundant pathways (iii) elucidation of the pathway by which P. aeruginosa can be internalized by non-phagocytic cells and how the type III secretion system-encoded effectors modulate entry (iv) characterization of novel genes involved in type IV pilin biogenesis and in the regulation of diverse virulence pathways (v) the first identification of a host cell ubiquitin ligase (cbl-b) that specifically targets the degradation of a type III secreted factor (vii) development of 2D and 3D cell-culture based systems to dissect the interaction of pathogens with the apical versus basolateral surface of polarized epithelial cells (vi) discovery that upon binding to the apical surface of polarized epithelial cells, P. aeruginosa forms biofilm-like structures that are able to transform apical membrane into basolateral membrane by exploiting the phosphatidyl inositol kinase pathway to form membrane protrusions that are associated with a spatial and temporal activation of the innate immune response. Our current studies focus on the dissection of the Chp/Vfr/ regulatory pathway that regulates diverse virulence factor circuits in P. aeruginosa in determining the bacterial and host determinants involved in the formation of biofilms and spatially localized activation of the innate immune response at the apical surface of tissues. In our studies on the pathogenesis of chlamydial infections, we have focused on host cell biology and genome-wide RNA-based screens to understanding how C. trachomatis modulates host cell signaling systems to bind, enter, and establish a replicative niche. We have carried out a genome wide RNAi screen in a simple genetic host and have identified new host molecules that are involved in binding, entry, and establishment of a unique intracellular niche. We have discovered a potential role for host growth factors in binding and entry and elucidated a novel pathway by which this organism acquires sphingolipids from the host. We have complemented these studies with state of the art confocal microscopy to begin to elucidate the bacterial and host determinants and mechanism of vacuole fusion. We are currently carrying out high throughput proteomics to dissect the function of the approximately 150 proteins that Chlamydia inject into the host cell to create a unique replicative niche and to escape the innate immune response.
Education & Training
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- Clinical and postdoctoral fellowship Infectious Disease University of California, San Francisco 1990
- Residency Internal Medicine University of Pennsylvania 1986
- M.D., PH.D. School of Medicine Stanford University 1983
Interests
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- Signal transduction (bacterial and eukaryotic)
- Proteomics
- Infectious Disease
- Cell Biology
- Pseudomonas aeruginosa
- Chlamydia
- Intracellular trafficking
- Host-pathogen interactions
- Microbial Pathogenesis
Grants and Projects
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- Microbial Pathogenesis and Host Defense, NIH, 2004-2024
- Understanding the role of sensory adaptation in bacterial mechanochemical signaling pathways, NIH, 2020-2022
- Inclusion membrane protein (Inc) modulation of the innate immune response to Chlamydia trachomatis, NIH, 2020-2021
- Adapting to a changing environment: How surface contact induces virulence factor production in Pseudomonas aeruginosa, NIH, 2017-2021
- Decoding the Chlamydia inclusion membrane protein-host protein interactome, NIH, 2015-2020
- Novel approaches to identify host genes required for Chlamydia pathogenesis, NIH, 2007-2018
- New approaches to study Pseudomonas-host interactions, NIH, 2005-2017
- Comprehensive Biology: Exploiting the Yeast Genome, NIH, 1996-2016
- High throughput proteomics to dissect Chlamydia-host cell interactions, NIH, 2013-2015
- New approaches to study Pseudomonas-host interactions, NIH, 2005-2015
- Mucosal Immune Barrier in Infection and Immunity, NIH, 2002-2014
- Mechanism of Pseudomonas-mediated epithelial cell damage, NIH, 1998-2012
- Proteomic approach to identify mediators of PI3K activation by P. aeruginosa, NIH, 2008-2012
- New approaches to studying host-chlamydia interactions, NIH, 2005-2007
- Biogenesis of the Chlamydia Trachomatis Vacuole, NIH, 2000-2006
- Molecular Biology of Chlamydial Development, NIH, 1988-2002
- Molecular and Cell Biology of Chlamydia Trachomatis, NIH, 1996-2001
- Cell Biology of Chlamydia, Trachomatis, NIH, 1996-1997
Publications (97)
Top publication keywords:
Chlamydia InfectionsPseudomonas aeruginosaBacterial AdhesionHost-Pathogen InteractionsADP Ribose TransferasesBacterial ProteinsVirulence FactorsFimbriae, BacterialPneumonia, BacterialPseudomonas InfectionsChlamydiaCell PolarityChlamydia trachomatisInclusion BodiesFimbriae Proteins
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Spatial control of sensory adaptation modulates mechanosensing in Pseudomonas aeruginosa.
bioRxiv : the preprint server for biology 2024 Patino R, Kühn MJ, Macmillan H, Inclan YF, Chavez I, Von Dollen J, Johnson JR, Swaney DL, Krogan NJ, Persat A, Engel JN -
Peptidoglycan-Targeted [18F]3,3,3-Trifluoro-d-alanine Tracer for Imaging Bacterial Infection.
JACS Au 2024 Alexandre M. Sorlin, Marina Lo´pez-A´lvarez, Jacob Biboy, Joe Gray, Sarah J. Rabbitt, Junaid Ur Rahim, Sang Hee Lee, Kondapa Naidu Bobba, Joseph Blecha, Mathew F.L. Parker, Robert R. Flavell, Joanne … -
Two antagonistic response regulators control Pseudomonas aeruginosa polarization during mechanotaxis.
The EMBO journal 2023 Kühn MJ, Macmillan H, Talà L, Inclan Y, Patino R, Pierrat X, Al-Mayyah Z, Engel JN, Persat A -
Imaging joint infections using D-methyl-11C-methionine PET/MRI: initial experience in humans.
European journal of nuclear medicine and molecular imaging 2022 Polvoy I, Seo Y, Parker M, Stewart M, Siddiqua K, Manacsa HS, Ravanfar V, Blecha J, Hope TA, Vanbrocklin H, Flavell RR, Barry J, Hansen E, Villanueva-Meyer JE, Engel J, Rosenberg OS, Wilson DM, … -
Mechanotaxis directs Pseudomonas aeruginosa twitching motility.
Proceedings of the National Academy of Sciences of the United States of America 2021 Kühn MJ, Talà L, Inclan YF, Patino R, Pierrat X, Vos I, Al-Mayyah Z, Macmillan H, Negrete J, Engel JN, Persat A
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Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
Nature microbiology 2019 Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS -
Both the N- and C- terminal regions of the Chlamydial inclusion protein D (IncD) are required for interaction with the pleckstrin homology domain of the ceramide transport protein CERT.
Biochemical and biophysical research communications 2018 Kumagai K, Elwell CA, Ando S, Engel JN, Hanada K -
Emerging Role of Retromer in Modulating Pathogen Growth.
Trends in microbiology 2018 Elwell C, Engel J -
Targeting the Mucosal Barrier: How Pathogens Modulate the Cellular Polarity Network.
Cold Spring Harbor perspectives in biology 2017 Ruch TR, Engel JN -
Chlamydia interfere with an interaction between the mannose-6-phosphate receptor and sorting nexins to counteract host restriction.
eLife 2017 Elwell CA, Czudnochowski N, von Dollen J, Johnson JR, Nakagawa R, Mirrashidi K, Krogan NJ, Engel JN, Rosenberg OS -
Bacterial internalization is required to trigger NIK-dependent NF-κB activation in response to the bacterial type three secretion system.
PloS one 2017 Duncan MC, Herrera NG, Johnson KS, Engel JN, Auerbuch V -
Par3 integrates Tiam1 and phosphatidylinositol 3-kinase signaling to change apical membrane identity.
Molecular biology of the cell 2016 Ruch TR, Bryant DM, Mostov KE, Engel JN -
A scaffold protein connects type IV pili with the Chp chemosensory system to mediate activation of virulence signaling in Pseudomonas aeruginosa.
Molecular microbiology 2016 Inclan YF, Persat A, Greninger A, Von Dollen J, Johnson J, Krogan N, Gitai Z, Engel JN -
Chlamydia cell biology and pathogenesis.
Nature reviews. Microbiology 2016 Elwell C, Mirrashidi K, Engel J -
Global Mapping of the Inc-Human Interactome Reveals that Retromer Restricts Chlamydia Infection.
Cell host & microbe 2015 Mirrashidi KM, Elwell CA, Verschueren E, Johnson JR, Frando A, Von Dollen J, Rosenberg O, Gulbahce N, Jang G, Johnson T, Jäger S, Gopalakrishnan AM, Sherry J, Dunn JD, Olive A, Penn B, Shales M, Cox … -
Type IV pili mechanochemically regulate virulence factors in Pseudomonas aeruginosa.
Proceedings of the National Academy of Sciences of the United States of America 2015 Persat A, Inclan YF, Engel JN, Stone HA, Gitai Z -
The Pseudomonas aeruginosa type III translocon is required for biofilm formation at the epithelial barrier.
PLoS pathogens 2014 Tran CS, Rangel SM, Almblad H, Kierbel A, Givskov M, Tolker-Nielsen T, Hauser AR, Engel JN -
Host cell polarity proteins participate in innate immunity to Pseudomonas aeruginosa infection.
Cell host & microbe 2014 Tran CS, Eran Y, Ruch TR, Bryant DM, Datta A, Brakeman P, Kierbel A, Wittmann T, Metzger RJ, Mostov KE, Engel JN -
Cystic fibrosis epithelial cell and bacterial binding.
American journal of physiology. Lung cellular and molecular physiology 2013 Engel J, Matthay M -
Sugar administration is an effective adjunctive therapy in the treatment of Pseudomonas aeruginosa pneumonia.
American journal of physiology. Lung cellular and molecular physiology 2013 Bucior I, Abbott J, Song Y, Matthay MA, Engel JN -
Chlamydial intracellular survival strategies.
Cold Spring Harbor perspectives in medicine 2013 Bastidas RJ, Elwell CA, Engel JN, Valdivia RH -
Endosulfatases SULF1 and SULF2 limit Chlamydia muridarum infection.
Cellular microbiology 2013 Kim JH, Chan C, Elwell C, Singer MS, Dierks T, Lemjabbar-Alaoui H, Rosen SD, Engel JN -
Lipid acquisition by intracellular Chlamydiae.
Cellular microbiology 2012 Elwell CA, Engel JN -
Pseudomonas aeruginosa pili and flagella mediate distinct binding and signaling events at the apical and basolateral surface of airway epithelium.
PLoS pathogens 2012 Bucior I, Pielage JF, Engel JN -
Chlamydia trachomatis co-opts the FGF2 signaling pathway to enhance infection.
PLoS pathogens 2011 Kim JH, Jiang S, Elwell CA, Engel JN -
Chlamydia trachomatis co-opts GBF1 and CERT to acquire host sphingomyelin for distinct roles during intracellular development.
PLoS pathogens 2011 Elwell CA, Jiang S, Kim JH, Lee A, Wittmann T, Hanada K, Melancon P, Engel JN -
Subversion of mucosal barrier polarity by pseudomonas aeruginosa.
Frontiers in microbiology 2011 Engel J, Eran Y -
Species-specific interactions of Src family tyrosine kinases regulate Chlamydia intracellular growth and trafficking.
mBio 2011 Elwell CA, Kierbel A, Engel JN -
Cell migration regulates the kinetics of cytokinesis.
Cell cycle (Georgetown, Tex.) 2011 Wood S, Sivaramakrishnan G, Engel J, Shafikhani SH -
FimL regulates cAMP synthesis in Pseudomonas aeruginosa.
PloS one 2011 Inclan YF, Huseby MJ, Engel JN -
Pseudomonas aeruginosa-mediated damage requires distinct receptors at the apical and basolateral surfaces of the polarized epithelium.
Infection and immunity 2009 Bucior I, Mostov K, Engel JN -
Genetic analysis of the regulation of type IV pilus function by the Chp chemosensory system of Pseudomonas aeruginosa.
Journal of bacteriology 2009 Bertrand JJ, West JT, Engel JN -
CbpA: a polarly localized novel cyclic AMP-binding protein in Pseudomonas aeruginosa.
Journal of bacteriology 2009 Endoh T, Engel JN -
Role of Pseudomonas aeruginosa type III effectors in disease.
Current opinion in microbiology 2009 Engel J, Balachandran P -
Focal adhesion components are essential for mammalian cell cytokinesis.
Cell cycle (Georgetown, Tex.) 2008 Shafikhani SH, Mostov K, Engel J -
Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms.
Environmental microbiology 2008 Barken KB, Pamp SJ, Yang L, Gjermansen M, Bertrand JJ, Klausen M, Givskov M, Whitchurch CB, Engel JN, Tolker-Nielsen T -
RNAi screen reveals an Abl kinase-dependent host cell pathway involved in Pseudomonas aeruginosa internalization.
PLoS pathogens 2008 Pielage JF, Powell KR, Kalman D, Engel JN -
RNA interference screen identifies Abl kinase and PDGFR signaling in Chlamydia trachomatis entry.
PLoS pathogens 2008 Elwell CA, Ceesay A, Kim JH, Kalman D, Engel JN -
The Pseudomonas aeruginosa type III secreted toxin ExoT is necessary and sufficient to induce apoptosis in epithelial cells.
Cellular microbiology 2007 Shafikhani SH, Morales C, Engel J -
Use of a novel coinfection system reveals a role for Rac1, H-Ras, and CrkII phosphorylation in Helicobacter pylori-induced host cell actin cytoskeletal rearrangements.
FEMS immunology and medical microbiology 2007 Brandt S, Shafikhani S, Balachandran P, Jin S, Hartig R, König W, Engel J, Backert S -
Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane.
The Journal of cell biology 2007 Kierbel A, Gassama-Diagne A, Rocha C, Radoshevich L, Olson J, Mostov K, Engel J -
Copper and nitric oxide meet in the plasma.
Nature chemical biology 2006 Samuel TK, Gitlin JD -
Drosophila melanogaster S2 cells: a model system to study Chlamydia interaction with host cells.
Cellular microbiology 2005 Elwell C, Engel JN -
An essential, putative MEK kinase of Leishmania major.
Molecular and biochemical parasitology 2005 Agron PG, Reed SL, Engel JN -
The phosphoinositol-3-kinase-protein kinase B/Akt pathway is critical for Pseudomonas aeruginosa strain PAK internalization.
Molecular biology of the cell 2005 Kierbel A, Gassama-Diagne A, Mostov K, Engel JN -
Pseudomonas aeruginosa fimL regulates multiple virulence functions by intersecting with Vfr-modulated pathways.
Molecular microbiology 2005 Whitchurch CB, Beatson SA, Comolli JC, Jakobsen T, Sargent JL, Bertrand JJ, West J, Klausen M, Waite LL, Kang PJ, Tolker-Nielsen T, Mattick JS, Engel JN -
Lipid raft-mediated entry is not required for Chlamydia trachomatis infection of cultured epithelial cells.
Infection and immunity 2004 Gabel BR, Elwell C, van Ijzendoorn SC, Engel JN -
A mycobacterial virulence gene cluster extending RD1 is required for cytolysis, bacterial spreading and ESAT-6 secretion.
Molecular microbiology 2004 Gao LY, Guo S, McLaughlin B, Morisaki H, Engel JN, Brown EJ -
Tarp and Arp: How Chlamydia induces its own entry.
Proceedings of the National Academy of Sciences of the United States of America 2004 Engel J -
Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa.
Molecular microbiology 2004 Whitchurch CB, Leech AJ, Young MD, Kennedy D, Sargent JL, Bertrand JJ, Semmler AB, Mellick AS, Martin PR, Alm RA, Hobbs M, Beatson SA, Huang B, Nguyen L, Commolli JC, Engel JN, Darzins A, Mattick JS -
The ADP ribosyltransferase domain of Pseudomonas aeruginosa ExoT contributes to its biological activities.
Infection and immunity 2004 Garrity-Ryan L, Shafikhani S, Balachandran P, Nguyen L, Oza J, Jakobsen T, Sargent J, Fang X, Cordwell S, Matthay MA, Engel JN -
Effector ExoU from the Type III Secretion System Is An Important Modulator of Gene Expression in Lung Epithelial Cells in Response to Pseudomonas aeruginosa Infection.
Infection and Immunity 2003 B. McMorran, L. Town, E. Costelloe, J. Palmer, J. Engel, D. Hume, B. Wainwright -
Epithelial cell polarity alters Rho-GTPase responses to Pseudomonas aeruginosa.
Molecular biology of the cell 2003 Kazmierczak BI, Mostov K, Engel JN -
Effector ExoU from the type III secretion system is an important modulator of gene expression in lung epithelial cells in response to Pseudomonas aeruginosa infection.
Infection and immunity 2003 McMorran B, Town L, Costelloe E, Palmer J, Engel J, Hume D, Wainwright B -
Molecular Pathogenesis of Acute Pseudomonas Aeruginosa Infections.
Severe Infections Caused by Pseudomonas Aeruginosa 2003 Joanne N. Engel -
Pseudomonas aeruginosa ExoT acts in vivo as a GTPase-activating protein for RhoA, Rac1, and Cdc42.
Infection and immunity 2002 Kazmierczak BI, Engel JN -
Type III protein secretion is associated with poor clinical outcomes in patients with ventilator-associated pneumonia caused by Pseudomonas aeruginosa.
Critical care medicine 2002 Hauser AR, Cobb E, Bodi M, Mariscal D, Vallés J, Engel JN, Rello J -
Pseudomonas aeruginosa ExoT inhibits in vitro lung epithelial wound repair.
Cellular microbiology 2001 Geiser TK, Kazmierczak BI, Garrity-Ryan LK, Matthay MA, Engel JN -
Rho GTPase activity modulates Pseudomonas aeruginosa internalization by epithelial cells.
Cellular microbiology 2001 Kazmierczak BI, Jou TS, Mostov K, Engel JN -
Interaction of bacterial pathogens with polarized epithelium.
Annual review of microbiology 2001 Kazmierczak BI, Mostov K, Engel JN -
Host cell-derived sphingolipids are required for the intracellular growth of Chlamydia trachomatis.
Cellular microbiology 2000 van Ooij C, Kalman L, Nishijima M, Hanada K, Mostov K, Engel JN -
The arginine finger domain of ExoT contributes to actin cytoskeleton disruption and inhibition of internalization of Pseudomonas aeruginosa by epithelial cells and macrophages.
Infection and immunity 2000 Garrity-Ryan L, Kazmierczak B, Kowal R, Comolli J, Hauser A, Engel JN -
Eukaryotic cell uptake of heparin-coated microspheres: a model of host cell invasion by Chlamydia trachomatis.
Infection and immunity 2000 Stephens RS, Fawaz FS, Kennedy KA, Koshiyama K, Nichols B, van Ooij C, Engel JN -
Pseudomonas aeruginosa induces type-III-secretion-mediated apoptosis of macrophages and epithelial cells.
Infection and immunity 1999 Hauser AR, Engel JN -
Pili binding to asialo-GM1 on epithelial cells can mediate cytotoxicity or bacterial internalization by Pseudomonas aeruginosa.
Infection and immunity 1999 Comolli JC, Waite LL, Mostov KE, Engel JN -
Pseudomonas aeruginosa gene products PilT and PilU are required for cytotoxicity in vitro and virulence in a mouse model of acute pneumonia.
Infection and immunity 1999 Comolli JC, Hauser AR, Waite L, Whitchurch CB, Mattick JS, Engel JN -
Fusion of Chlamydia trachomatis-containing inclusions is inhibited at low temperatures and requires bacterial protein synthesis.
Infection and immunity 1998 Van Ooij C, Homola E, Kincaid E, Engel J -
Mutational analysis of the Chlamydia trachomatis rRNA P1 promoter defines four regions important for transcription in vitro.
Journal of bacteriology 1998 Tan M, Gaal T, Gourse RL, Engel JN -
Defects in type III secretion correlate with internalization of Pseudomonas aeruginosa by epithelial cells.
Infection and immunity 1998 Hauser AR, Fleiszig S, Kang PJ, Mostov K, Engel JN -
PepA, a secreted protein of Pseudomonas aeruginosa, is necessary for cytotoxicity and virulence.
Molecular microbiology 1998 Hauser AR, Kang PJ, Engel JN -
Infection with Chlamydia trachomatis alters the tyrosine phosphorylation and/or localization of several host cell proteins including cortactin.
Infection and immunity 1997 Fawaz FS, van Ooij C, Homola E, Mutka SC, Engel JN -
Identification of Pseudomonas aeruginosa genes required for epithelial cell injury.
Molecular microbiology 1997 Kang PJ, Hauser AR, Apodaca G, Fleiszig SM, Wiener-Kronish J, Mostov K, Engel JN -
Characterization of the Chlamydia trachomatis vacuole and its interaction with the host endocytic pathway in HeLa cells.
Infection and immunity 1997 van Ooij C, Apodaca G, Engel J -
Identification of sequences necessary for transcription in vitro from the Chlamydia trachomatis rRNA P1 promoter.
Journal of bacteriology 1996 Tan M, Engel JN -
Transcriptional organization and regulation of the dnaK and groE operons of Chlamydia trachomatis.
Journal of bacteriology 1996 Tan M, Wong B, Engel JN -
Characterization of Pseudomonas aeruginosa-induced MDCK cell injury: glycosylation-defective host cells are resistant to bacterial killing.
Infection and immunity 1995 Apodaca G, Bomsel M, Lindstedt R, Engel J, Frank D, Mostov KE, Wiener-Kronish J -
Stage-specific regulation of protein phosphorylation in Leishmania major.
Molecular and biochemical parasitology 1994 Dell KR, Engel JN -
Cloning and characterization of the RNA polymerase alpha-subunit operon of Chlamydia trachomatis.
Journal of bacteriology 1993 Tan M, Klein R, Grant R, Ganem D, Engel J -
Azithromycin-induced block of elementary body formation in Chlamydia trachomatis.
Antimicrobial agents and chemotherapy 1992 Engel JN -
A developmentally regulated chlamydial gene with apparent homology to eukaryotic histone H1.
Proceedings of the National Academy of Sciences of the United States of America 1992 Perara E, Ganem D, Engel JN -
Heat shock response of murine Chlamydia trachomatis.
Journal of bacteriology 1990 Engel JN, Pollack J, Perara E, Ganem D -
Cloning and characterization of RNA polymerase core subunits of Chlamydia trachomatis by using the polymerase chain reaction.
Journal of bacteriology 1990 Engel JN, Pollack J, Malik F, Ganem D -
A polymerase chain reaction-based approach to cloning sigma factors from eubacteria and its application to the isolation of a sigma-70 homolog from Chlamydia trachomatis.
Journal of bacteriology 1990 Engel JN, Ganem D -
21 Identification and Comparison of Putative Chlamydial Promoter Elements.
Immune Recognition and Evasion: Molecular Aspects of Host?parasite Interaction 1990 JOANNE N. ENGEL, DON GANEM -
Chlamydial gene encoding a 70-kilodalton antigen in Escherichia coli: analysis of expression signals and identification of the gene product.
Journal of bacteriology 1989 Sardinia LM, Engel JN, Ganem D -
Chlamydial rRNA operons: gene organization and identification of putative tandem promoters.
Journal of bacteriology 1987 Engel JN, Ganem D -
Phenytoin hypersensitivity: a case of severe acute rhabdomyolysis.
The American journal of medicine 1986 Engel JN, Mellul VG, Goodman DB -
Phenytoin hypersensitivity: a case of severe acute rhabdomyolysis
American Journal of Medicine 1986 Engel, JN, Mellul, VG, Goodman, DBP -
Evolutionary conservation in the untranslated regions of actin mRNA's
Nucleic Acids Research 1984 Ponte, P, Ng, SY, Engel, JN, Kedes, LH -
Isolation and characterization of full-length cDNA clones for human a-, b-, and c- actin mRNA's: skeletal but not cytoplasmic actins have an amino terminal cysteine that is subsequently removed
Molecular and Cellular Biology 1983 Gunning, P, Ponte, P, Okayama, H, Engel, JN, Kedes, L -
Isolation and characterization of human actin genes. Ph.D. Dissertation, Stanford University
Isolation and characterization of human actin genes. Ph.D. Dissertation, Stanford University 1983 Engel, JN -
Molecular genetics of human myogenesis
Experimental Biology and Medicine 1983 Kedes, LH, Blau, H, Gunning, P, Ponte, P, Chiu, C-P, Bain, W, Engel, JN, Guttman, S -
Human cytoplasmic actin proteins are encoded by a multigene family
Molecular and Cellular Biology 1982 Engel, JN, Gunning, P, Kedes, LH -
Isolation and characterization of human actin genes.
Proceedings of the National Academy of Sciences of the United States of America 1981 Engel JN, Gunning PW, Kedes L -
Isolation and characterization of human actin genes
Proceedings National Academy of Sciences 1981 Engel, JN, Gunning, P, Kedes, LH -
Inhibition of the in vitro pituitary response to luteinizing hormone-releasing hormone by melatonin, serotonin, and 5-methoxytryptamine.
Endocrinology 1977 Martin JE, Engel JN, Klein DC -
Inhibition of the in vitro pituitary response to luteinizing hormone releasing hormone by melatonin, serotonin, and 5-methoxytryptamine
Endocrinology 1977 Martin, JE, Engel, JN, Klein, DC