Jeroen Roose, PhD
Professor
Anatomy
School of Medicine

Our laboratory focuses on understanding cell fate decisions driven by cell-cell interactions and signaling pathways, in the context of immunology, cancer, and stem cell biology. We have a very international team that embraces DEI. Post docs in my lab go off to start their own academic labs or secure exciting positions in industry. http://rooselab.ucsf.edu/

In addition to running our lab, I enjoy setting up large collaborative research programs so that my team members (and everyone else) can benefit. In 2018, I co-founded ImmunoX. Also in 2018, I started an Organoid disease to biology (D2B) unit. I co-lead the UCSF Endeavor Program that focuses on cancer metastasis and co-lead the UCSF AutoImmunoProfiler Initiative.

• SARS-CoV-2 infection of airway organoids reveals conserved use of Tetraspanin-8 by Ancestral, Delta, and Omicron variants.
  Stem cell reports 2023 Hysenaj L, Little S, Kulhanek K, Magnen M, Bahl K, Gbenedio OM, Prinz M, Rodriguez L, Andersen C, Rao AA, Shen A, Lone JC, Lupin-Jimenez LC, Bonser LR, Serwas NK, Mick E, Khalid MM, Taha TY, Kumar R, …
• Abstract IA022: Cell archetypes in human brain metastases: Connecting programs in tumor cells with the microenvironment.
  Cancer Research 2023 Hugo Gonzalez, Wenbin Mei, Isabella Robles, Catharina Hagerling, Breanna Allen, Trine Hauge Okholm, Ankitha Nanjaraj, Tamara Verbeek, Sandhya Kalavacherla, Merel van Gogh, Stephen Georgiou, Mariza …
• NF-κB inhibitor alpha has a cross-variant role during SARS-CoV-2 infection in ACE2-overexpressing human airway organoids.
  bioRxiv : the preprint server for biology 2022 Simoneau CR, Chen PY, Xing GK, Khalid MM, Meyers NL, Hayashi JM, Taha TY, Leon KE, Ashuach T, Fontaine KA, Rodriguez L, Joehnk B, Walcott K, Vasudevan S, Fang X, Maishan M, Schultz S, Roose J, Matthay…
• Immediate myeloid depot for SARS-CoV-2 in the human lung.
  Research square 2022 Magnen M, You R, Rao AA, Davis RT, Rodriguez L, Simoneau CR, Hysenaj L, Hu KH, UCSF COMET Consortium, Love C, Woodruff PG, Erle DJ, Hendrickson CM, Calfee CS, Matthay MA, Roose JP, Sil A, Ott M, …
• Immediate myeloid depot for SARS-CoV-2 in the human lung.
  bioRxiv : the preprint server for biology 2022 Magnen M, You R, Rao AA, Davis RT, Rodriguez L, Simoneau CR, Hysenaj L, Hu KH, UCSF COMET Consortium, Love C, Woodruff PG, Erle DJ, Hendrickson CM, Calfee CS, Matthay MA, Roose JP, Sil A, Ott M, …
• Tcof1 haploinsufficiency promotes early T cell precursor-like leukemia in NrasQ61R/+ mice.
  Leukemia 2022 Wen Z, Finn R, Gao X, Li L, Hebert A, Ranheim EA, Zhou Y, Yun G, Roose JP, Coon JJ, Shiang R, Wen R, Yu M, Wang D, Zhang J
• Cellular architecture of human brain metastases.
  Cell 2022 Gonzalez H, Mei W, Robles I, Hagerling C, Allen BM, Hauge Okholm TL, Nanjaraj A, Verbeek T, Kalavacherla S, van Gogh M, Georgiou S, Daras M, Phillips JJ, Spitzer MH, Roose JP, Werb Z
• Editorial overview: Communities in immune cell signaling.
  Current opinion in immunology 2021 Roose J, Barton G
• 603 Autoreactivity drives increased metabolism in T cells from SLE patients?.
  Lupus Science & Medicine 2021 Kriti Bahl, Darienne Myers, Jeroen Roose
• Nras Q61R/+ and Kras-/- cooperate to downregulate Rasgrp1 and promote lympho-myeloid leukemia in early T-cell precursors.
  Blood 2021 Wen Z, Yun G, Hebert A, Kong G, Ranheim EA, Finn R, Rajagoplan A, Li S, Zhou Y, Yu M, Damnernsawad A, Roose JP, Coon JJ, Wen R, Wang D, Zhang J
• SARS-CoV-2 infection studies in lung organoids identify TSPAN8 as novel mediator.
  bioRxiv : the preprint server for biology 2021 Hysenaj L, Little S, Kulhanek K, Gbenedio OM, Rodriguez L, Shen A, Lone JC, Lupin-Jimenez LC, Bonser LR, Serwas NK, Bahl K, Mick E, Li JZ, Ding VW, Matsumoto S, Maishan M, Simoneau C, Fragiadakis G, …
• T cells: a dedicated effector kinase pathways for every trait?
  The Biochemical journal 2021 Bahl K, Roose JP
• Investigating increased hematopoietic stem cell fitness in a novel mouse model.
  Small GTPases 2021 Karra L, Roose JP
• Bioinformatic Approaches to Validation and Functional Analysis of 3D Lung Cancer Models.
  Cancers 2021 Li PJ, Roose JP, Jablons DM, Kratz JR
• Regulation of the Small GTPase Ras and Its Relevance to Human Disease.
  Methods in molecular biology (Clifton, N.J.) 2021 Kulhanek KR, Roose JP, Rubio I
• Dysregulated RASGRP1 expression through RUNX1 mediated transcription promotes autoimmunity.
  European journal of immunology 2020 Baars MJD, Douma T, Simeonov DR, Myers DR, Kulhanek K, Banerjee S, Zwakenberg S, Baltissen MP, Amini M, de Roock S, van Wijk F, Vermeulen M, Marson A, Roose JP, Vercoulen Y
• Increased baseline RASGRP1 signals enhance stem cell fitness during native hematopoiesis.
  Oncogene 2020 Karra L, Romero-Moya D, Ksionda O, Krush M, Gu Z, Mues M, Depeille P, Mullighan C, Roose JP
• Next-Generation Surrogate Wnts Support Organoid Growth and Deconvolute Frizzled Pleiotropy In Vivo.
  Cell stem cell 2020 Miao Y, Ha A, de Lau W, Yuki K, Santos AJM, You C, Geurts MH, Puschhof J, Pleguezuelos-Manzano C, Peng WC, Senlice R, Piani C, Buikema JW, Gbenedio OM, Vallon M, Yuan J, de Haan S, Hemrika W, Rösch K,…
• Protocol for Barcoding T Cells Combined with Timed Stimulations.
  STAR protocols 2020 Kulhanek KR, Myers DR, Ksionda O, Vercoulen Y, Romero-Moya D, Roose JP
• Protocol for Comprehensive Synthetic Lethality Screens.
  STAR protocols 2020 Romero-Moya D, Roose JP
• Unraveling Heterogeneity in Epithelial Cell Fates of the Mammary Gland and Breast Cancer.
  Cancers 2019 Samocha A, Doh H, Kessenbrock K, Roose JP
• Cryo-EM structure of a dimeric B-Raf:14-3-3 complex reveals asymmetry in the active sites of B-Raf kinases.
  Science (New York, N.Y.) 2019 Kondo Y, Ognjenovic J, Banerjee S, Karandur D, Merk A, Kulhanek K, Wong K, Roose JP, Subramaniam S, Kuriyan J
• mTOR and other effector kinase signals that impact T cell function and activity.
  Immunological reviews 2019 Myers DR, Wheeler B, Roose JP
• Alternative ZAP70-p38 signals prime a classical p38 pathway through LAT and SOS to support regulatory T cell differentiation.
  Science signaling 2019 Jun JE, Kulhanek KR, Chen H, Chakraborty A, Roose JP
• RasGRP1 is a potential biomarker to stratify anti-EGFR therapy response in colorectal cancer.
  JCI insight 2019 Gbenedio OM, Bonnans C, Grun D, Wang CY, Hatch AJ, Mahoney MR, Barras D, Matli M, Miao Y, Garcia KC, Tejpar S, Delorenzi M, Venook AP, Nixon AB, Warren RS, Roose JP, Depeille P
• Active Tonic mTORC1 Signals Shape Baseline Translation in Naive T Cells.
  Cell reports 2019 Myers DR, Norlin E, Vercoulen Y, Roose JP
• High-Complexity shRNA Libraries and PI3 Kinase Inhibition in Cancer: High-Fidelity Synthetic Lethality Predictions.
  Cell reports 2019 Mues M, Karra L, Romero-Moya D, Wandler A, Hangauer MJ, Ksionda O, Thus Y, Lindenbergh M, Shannon K, McManus MT, Roose JP
• Comprehensive analysis of T cell leukemia signals reveals heterogeneity in the PI3 kinase-Akt pathway and limitations of PI3 kinase inhibitors as monotherapy.
  PloS one 2018 Ksionda O, Mues M, Wandler AM, Donker L, Tenhagen M, Jun J, Ducker GS, Matlawska-Wasowska K, Shannon K, Shokat KM, Roose JP
• A Histidine pH sensor regulates activation of the Ras-specific guanine nucleotide exchange factor RasGRP1.
  eLife 2017 Vercoulen Y, Kondo Y, Iwig JS, Janssen AB, White KA, Amini M, Barber DL, Kuriyan J, Roose JP
• Tonic Signals: Why Do Lymphocytes Bother?
  Trends in immunology 2017 Myers DR, Zikherman J, Roose JP
• Tonic LAT-HDAC7 Signals Sustain Nur77 and Irf4 Expression to Tune Naive CD4 T Cells.
  Cell reports 2017 Myers DR, Lau T, Markegard E, Lim HW, Kasler H, Zhu M, Barczak A, Huizar JP, Zikherman J, Erle DJ, Zhang W, Verdin E, Roose JP
• RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma.
  Cancer cell 2017 Chen X, Wu Q, Depeille P, Chen P, Thornton S, Kalirai H, Coupland SE, Roose JP, Bastian BC
• Lost GRP on cytotoxicity?
  Nature immunology 2016 Roose JP
• One-way membrane trafficking of SOS in receptor-triggered Ras activation.
  Nature structural & molecular biology 2016 Christensen SM, Tu HL, Jun JE, Alvarez S, Triplet MG, Iwig JS, Yadav KK, Bar-Sagi D, Roose JP, Groves JT
• Distinct oncogenic Ras signals characterized by profound differences in flux through the RasGDP/RasGTP cycle.
  Small GTPases 2016 Mues M, Roose JP
• Abstract B22: Screening and validation of combination therapy in T cell leukemia.
  Technologies Used in Basic Cancer Research 2016 Marsilius Mues, Marthe F. Lindenbergh, Michael T. McManus, Jeroen P. Roose
• Suppressive signals in colorectal cancer: RasGRP1 a new marker for anti-EGFR therapy?.
  Journal of Clinical Oncology 2016 Jeroen Roose, Philippe Depeille, Robert S. Warren
• Human iPS Cell-Derived Neurons Uncover the Impact of Increased Ras Signaling in Costello Syndrome.
  The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Rooney GE, Goodwin AF, Depeille P, Sharir A, Schofield CM, Yeh E, Roose JP, Klein OD, Rauen KA, Weiss LA, Ullian EM
• Kinase and Phosphatase Effector Pathways in T Cells.
  Encyclopedia of Immunobiology 2016 Darienne R. Myers, Jeroen P. Roose
• RasGRP1 overexpression in T-ALL increases basal nucleotide exchange on Ras rendering the Ras/PI3K/Akt pathway responsive to protumorigenic cytokines.
  Oncogene 2015 Ksionda O, Melton AA, Bache J, Tenhagen M, Bakker J, Harvey R, Winter SS, Rubio I, Roose JP
• Unexpected insights for anti-EGFR cancer therapy.
  Oncotarget 2015 Depeille P, Warren RS, Roose JP
• RasGRP1 opposes proliferative EGFR-SOS1-Ras signals and restricts intestinal epithelial cell growth.
  Nature cell biology 2015 Depeille P, Henricks LM, van de Ven RA, Lemmens E, Wang CY, Matli M, Werb Z, Haigis KM, Donner D, Warren R, Roose JP
• Cutting Edge: Codeletion of the Ras GTPase-Activating Proteins (RasGAPs) Neurofibromin 1 and p120 RasGAP in T Cells Results in the Development of T Cell Acute Lymphoblastic Leukemia.
  Journal of immunology (Baltimore, Md. : 1950) 2015 Lubeck BA, Lapinski PE, Oliver JA, Ksionda O, Parada LF, Zhu Y, Maillard I, Chiang M, Roose J, King PD
• Editorial overview: lymphocyte development and activation: lymphocytes, integrators of information.
  Current opinion in immunology 2015 Roose J, Fagarasan S
• Flavors of EGFR-Ras signals impacting intestinal homeostasis.
  Cell cycle (Georgetown, Tex.) 2015 Depeille P, Roose JP
• Protein kinase Cδ promotes transitional B cell-negative selection and limits proximal B cell receptor signaling to enforce tolerance.
  Molecular and cellular biology 2014 Limnander A, Zikherman J, Lau T, Leitges M, Weiss A, Roose JP
• Rasgrp1 mutation increases naive T-cell CD44 expression and drives mTOR-dependent accumulation of Helios⁺ T cells and autoantibodies.
  eLife 2013 Daley SR, Coakley KM, Hu DY, Randall KL, Jenne CN, Limnander A, Myers DR, Polakos NK, Enders A, Roots C, Balakishnan B, Miosge LA, Sjollema G, Bertram EM, Field MA, Shao Y, Andrews TD, Whittle B, …
• PLC-γ and PI3K link cytokines to ERK activation in hematopoietic cells with normal and oncogenic Kras.
  Science signaling 2013 Diaz-Flores E, Goldschmidt H, Depeille P, Ng V, Akutagawa J, Krisman K, Crone M, Burgess MR, Williams O, Houseman B, Shokat K, Sampath D, Bollag G, Roose JP, Braun BS, Shannon K
• RasGRP Ras guanine nucleotide exchange factors in cancer.
  Frontiers in biology 2013 Ksionda O, Limnander A, Roose JP
• Regulation of ras exchange factors and cellular localization of ras activation by lipid messengers in T cells.
  Frontiers in immunology 2013 Jun JE, Rubio I, Roose JP
• Structural analysis of autoinhibition in the Ras-specific exchange factor RasGRP1.
  eLife 2013 Iwig JS, Vercoulen Y, Das R, Barros T, Limnander A, Che Y, Pelton JG, Wemmer DE, Roose JP, Kuriyan J
• Biochemical heterogeneity and developmental varieties in T-cell leukemia.
  Cell cycle (Georgetown, Tex.) 2013 Chakraborty AK, Roose JP
• Activation of extracellular signal-regulated kinase but not of p38 mitogen-activated protein kinase pathways in lymphocytes requires allosteric activation of SOS.
  Molecular and cellular biology 2013 Jun JE, Yang M, Chen H, Chakraborty AK, Roose JP
• Dysregulated RasGRP1 responds to cytokine receptor input in T cell leukemogenesis.
  Science signaling 2013 Hartzell C, Ksionda O, Lemmens E, Coakley K, Yang M, Dail M, Harvey RC, Govern C, Bakker J, Lenstra TL, Ammon K, Boeter A, Winter SS, Loh M, Shannon K, Chakraborty AK, Wabl M, Roose JP
• A phospholipase C-γ1-independent, RasGRP1-ERK-dependent pathway drives lymphoproliferative disease in linker for activation of T cells-Y136F mutant mice.
  Journal of immunology (Baltimore, Md. : 1950) 2012 Kortum RL, Rouquette-Jazdanian AK, Miyaji M, Merrill RK, Markegard E, Pinski JM, Wesselink A, Nath NN, Alexander CP, Li W, Kedei N, Roose JP, Blumberg PM, Samelson LE, Sommers CL
• The membrane environment can promote or suppress bistability in cell signaling networks.
  The journal of physical chemistry. B 2012 Abel SM, Roose JP, Groves JT, Weiss A, Chakraborty AK
• Basal LAT-diacylglycerol-RasGRP1 signals in T cells maintain TCRα gene expression.
  PloS one 2011 Markegard E, Trager E, Yang CW, Zhang W, Weiss A, Roose JP
• Stim1, PKCd and RasGRP proteins set a threshold for pro-apoptotic Erk signaling during B cell development (109.6).
  The Journal of Immunology 2011 Andre Limnander, Philippe Depeille, Tanya Freedman, Jen Liou, Michael Leitges, Tomohiro Kurosaki, Jeroen Roose, Arthur Weiss
• STIM1, PKC-δ and RasGRP set a threshold for proapoptotic Erk signaling during B cell development.
  Nature immunology 2011 Limnander A, Depeille P, Freedman TS, Liou J, Leitges M, Kurosaki T, Roose JP, Weiss A
• Abstract 3837: PI-3 kinase acts upstream of oncogenic Ras in hematopoietic cells through a cytokine-PLC?-RasGRP pathway.
  Cellular and Molecular Biology 2010 Ernesto Diaz-Flores, Hana Goldschmidt, Philippe Depeille, Victor Ng, Jeroen Roose, Kevan Shokat, Benjamin Braun, Kevin Shannon
• PI3 Kinase, Phospholipase C (PLC)-?, and RasGRPs Act Cooperatively to Activate the Ras-Extracellular-Related Kinase (ERK) Pathway in Response to Cytokines in Normal and Kras Mutant Myeloid Cells.
  Blood 2009 Ernesto Diaz-Flores, Hana Leah Goldschmidt, Philippe Depeille, Victor Ng, Jeroen Roose, Kevan Shokat, Benjamin S. Braun, Kevin Shannon
• Response and resistance to MEK inhibition in leukaemias initiated by hyperactive Ras.
  Nature 2009 Lauchle JO, Kim D, Le DT, Akagi K, Crone M, Krisman K, Warner K, Bonifas JM, Li Q, Coakley KM, Diaz-Flores E, Gorman M, Przybranowski S, Tran M, Kogan SC, Roose JP, Copeland NG, Jenkins NA, Parada L, …
• Molecular origin and functional consequences of digital signaling and hysteresis during Ras activation in lymphocytes.
  Science signaling 2009 Chakraborty AK, Das J, Zikherman J, Yang M, Govern CC, Ho M, Weiss A, Roose J
• Digital signaling and hysteresis characterize ras activation in lymphoid cells.
  Cell 2009 Das J, Ho M, Zikherman J, Govern C, Yang M, Weiss A, Chakraborty AK, Roose JP
• Origin of the sharp boundary that discriminates positive and negative selection of thymocytes.
  Proceedings of the National Academy of Sciences of the United States of America 2008 Prasad A, Zikherman J, Das J, Roose JP, Weiss A, Chakraborty AK
• Unusual interplay of two types of Ras activators, RasGRP and SOS, establishes sensitive and robust Ras activation in lymphocytes.
  Molecular and cellular biology 2007 Roose JP, Mollenauer M, Ho M, Kurosaki T, Weiss A
• Phosphoinositide-dependent kinase 1 targets protein kinase A in a pathway that regulates interleukin 4.
  The Journal of experimental medicine 2006 Nirula A, Ho M, Phee H, Roose J, Weiss A
• A diacylglycerol-protein kinase C-RasGRP1 pathway directs Ras activation upon antigen receptor stimulation of T cells.
  Molecular and cellular biology 2005 Roose JP, Mollenauer M, Gupta VA, Stone J, Weiss A
• Intramolecular regulatory switch in ZAP-70: analogy with receptor tyrosine kinases.
  Molecular and cellular biology 2005 Brdicka T, Kadlecek TA, Roose JP, Pastuszak AW, Weiss A
• T cell receptor-independent basal signaling via Erk and Abl kinases suppresses RAG gene expression.
  PLoS biology 2003 Roose JP, Diehn M, Tomlinson MG, Lin J, Alizadeh AA, Botstein D, Brown PO, Weiss A
• All Tcf HMG box transcription factors interact with Groucho-related co-repressors.
  Nucleic acids research 2001 Brantjes H, Roose J, van De Wetering M, Clevers H
• T cells: getting a GRP on Ras.
  Nature immunology 2000 Roose J, Weiss A
• dTcf antagonises Wingless signalling during the development and patterning of the wing in Drosophila.
  The International journal of developmental biology 2000 Lawrence N, Dearden P, Hartley D, Roose J, Clevers H, Arias AM
• Differential expression of the Groucho-related genes 4 and 5 during early development of Xenopus laevis.
  Mechanisms of development 2000 Molenaar M, Brian E, Roose J, Clevers H, Destrée O
• TCF transcription factors: molecular switches in carcinogenesis.
  Biochimica et biophysica acta 1999 Roose J, Clevers H
• Synergy between tumor suppressor APC and the beta-catenin-Tcf4 target Tcf1.
  Science (New York, N.Y.) 1999 Roose J, Huls G, van Beest M, Moerer P, van der Horn K, Goldschmeding R, Logtenberg T, Clevers H
• The Sox-13 gene: structure, promoter characterization, and chromosomal localization.
  Genomics 1999 Roose J, Korver W, de Boer R, Kuipers J, Hurenkamp J, Clevers H
• Drosophila Tcf and Groucho interact to repress Wingless signalling activity.
  Nature 1998 Cavallo RA, Cox RT, Moline MM, Roose J, Polevoy GA, Clevers H, Peifer M, Bejsovec A
• The Xenopus Wnt effector XTcf-3 interacts with Groucho-related transcriptional repressors.
  Nature 1998 Roose J, Molenaar M, Peterson J, Hurenkamp J, Brantjes H, Moerer P, van de Wetering M, Destrée O, Clevers H
• Differential expression of the HMG box transcription factors XTcf-3 and XLef-1 during early xenopus development.
  Mechanisms of development 1998 Molenaar M, Roose J, Peterson J, Venanzi S, Clevers H, Destrée O
• Two members of the Tcf family implicated in Wnt/beta-catenin signaling during embryogenesis in the mouse.
  Molecular and cellular biology 1998 Korinek V, Barker N, Willert K, Molenaar M, Roose J, Wagenaar G, Markman M, Lamers W, Destree O, Clevers H
• High expression of the HMG box factor sox-13 in arterial walls during embryonic development.
  Nucleic acids research 1998 Roose J, Korver W, Oving E, Wilson A, Wagenaar G, Markman M, Lamers W, Clevers H
• The human TRIDENT/HFH-11/FKHL16 gene: structure, localization, and promoter characterization.
  Genomics 1997 Korver W, Roose J, Heinen K, Weghuis DO, de Bruijn D, van Kessel AG, Clevers H
• The winged-helix transcription factor Trident is expressed in actively dividing lymphocytes.
  Immunobiology 1997 Korver W, Roose J, Wilson A, Clevers H
• The winged-helix transcription factor Trident is expressed in cycling cells.
  Nucleic acids research 1997 Korver W, Roose J, Clevers H
• XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos.
  Cell 1996 Molenaar M, van de Wetering M, Oosterwegel M, Peterson-Maduro J, Godsave S, Korinek V, Roose J, Destrée O, Clevers H
• Characterization of the gene encoding the polymorphic immunodominant molecule, a neutralizing antigen of Theileria parva.
  Journal of immunology (Baltimore, Md. : 1950) 1995 Toye PG, Metzelaar MJ, Wijngaard PL, Nene V, Iams K, Roose J, Nyanjui JK, Gobright E, Musoke AJ, Clevers HC
• Synthetic protease inhibitors: promising compounds to arrest pathobiologic processes.
  The Journal of laboratory and clinical medicine 1995 Roose JP, Van Noorden CJ