Halina Ewa Witkowski, PhD
Professor Emeritus
Ob/Gyn, Reproductive Sciences
School of Medicine

Grants and Projects

Show all (1) Hide

• Structural Characterization of Polypeptides, Protein Complexes, Oligosaccharides, NIH, 2010-2012

Publications (49)

Top publication keywords:
Desulfovibrio vulgarisNeoplasms, ExperimentalPeptidesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTandem Mass SpectrometryHigh-Throughput Screening AssaysMultiprotein ComplexesProteomicsParotid GlandChromatography, LiquidFatty Acid SynthasesMass Spectrometry1,2-DipalmitoylphosphatidylcholineSpectrometry, Mass, Electrospray IonizationBacterial Outer Membrane Proteins

• High-throughput isolation and characterization of untagged membrane protein complexes: outer membrane complexes of Desulfovibrio vulgaris.
  Journal of proteome research 2012 Walian PJ, Allen S, Shatsky M, Zeng L, Szakal ED, Liu H, Hall SC, Fisher SJ, Lam BR, Singer ME, Geller JT, Brenner SE, Chandonia JM, Hazen TC, Witkowska HE, Biggin MD, Jap BK
• Lectin chromatography/mass spectrometry discovery workflow identifies putative biomarkers of aggressive breast cancers.
  Journal of proteome research 2012 Drake PM, Schilling B, Niles RK, Prakobphol A, Li B, Jung K, Cho W, Braten M, Inerowicz HD, Williams K, Albertolle M, Held JM, Iacovides D, Sorensen DJ, Griffith OL, Johansen E, Zawadzka AM, Cusack MP…
• Cells Tissues Organs
  Amelogenin Exons 8 and 9 Encoded Peptide Enhances Leucine Rich Amelogenin Peptide Mediated Dental Pulp Repair 2012 Huang Y, Goldberg M, Le T, Qiang R, Warner D, Witkowska HE, Liu H, Zhu L, Denbesten P, and Li W.
• Peptides released by physiological cleavage of semen coagulum proteins form amyloids that enhance HIV infection.
  Cell host & microbe 2011 Roan NR, Müller JA, Liu H, Chu S, Arnold F, Stürzel CM, Walther P, Dong M, Witkowska HE, Kirchhoff F, Münch J, Greene WC
• Breaking the bottleneck in the protein biomarker pipeline.
  Clinical chemistry 2011 Witkowska HE, Hall SC, Fisher SJ

Show all (44 more) Hide

• Evidence-based annotation of transcripts and proteins in the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough.
  Journal of bacteriology 2011 Price MN, Deutschbauer AM, Kuehl JV, Liu H, Witkowska HE, Arkin AP
• Automated iterative MS/MS acquisition: a tool for improving efficiency of protein identification using a LC-MALDI MS workflow.
  Analytical chemistry 2011 Liu H, Yang L, Khainovski N, Dong M, Hall SC, Fisher SJ, Biggin MD, Jin J, Witkowska HE
• ABRF-PRG07: advanced quantitative proteomics study.
  Journal of biomolecular techniques : JBT 2011 Falick AM, Lane WS, Lilley KS, MacCoss MJ, Phinney BS, Sherman NE, Weintraub ST, Witkowska HE, Yates NA
• The ABRF Proteomics Research Group studies: educational exercises for qualitative and quantitative proteomic analyses.
  Proteomics 2011 Friedman DB, Andacht TM, Bunger MK, Chien AS, Hawke DH, Krijgsveld J, Lane WS, Lilley KS, MacCoss MJ, Moritz RL, Settlage RE, Sherman NE, Weintraub ST, Witkowska HE, Yates NA, Turck CW
• Arch Oral Biol
  Hydrolysis of amelogenin by matrix metalloprotease-20 accelerates mineralization in vitro 2011 Uskokovic V, Khan F, Liu H, Witkowska HE, Zhu L, Li W, and Habelitz S.
• Eur J Oral Sci
  Fluoride incorporation into apatite crystals delays amelogenin hydrolysis 2011 Denbesten PK, Zhu L, Li W, Tanimoto K, Liu H, and Witkowska HE
• A lectin affinity workflow targeting glycosite-specific, cancer-related carbohydrate structures in trypsin-digested human plasma.
  Analytical biochemistry 2010 Drake PM, Schilling B, Niles RK, Braten M, Johansen E, Liu H, Lerch M, Sorensen DJ, Li B, Allen S, Hall SC, Witkowska HE, Regnier FE, Gibson BW, Fisher SJ
• Survey of large protein complexes in D. vulgaris reveals great structural diversity.
  Proceedings of the National Academy of Sciences of the United States of America 2009 Han BG, Dong M, Liu H, Camp L, Geller J, Singer M, Hazen TC, Choi M, Witkowska HE, Ball DA, Typke D, Downing KH, Shatsky M, Brenner SE, Chandonia JM, Biggin MD, Glaeser RM
• The sodium-hydrogen exchanger NHE1 is an Akt substrate necessary for actin filament reorganization by growth factors.
  The Journal of biological chemistry 2009 Meima ME, Webb BA, Witkowska HE, Barber DL
• Acid-catalyzed oxygen-18 labeling of peptides.
  Analytical chemistry 2009 Niles R, Witkowska HE, Allen S, Hall SC, Fisher SJ, Hardt M
• Rapid alterations in cortical protein profiles underlie spontaneous sleep and wake bouts.
  Journal of cellular biochemistry 2008 Vazquez J, Hall SC, Witkowska HE, Greco MA
• The proteomes of human parotid and submandibular/sublingual gland salivas collected as the ductal secretions.
  Journal of proteome research 2008 Denny P, Hagen FK, Hardt M, Liao L, Yan W, Arellanno M, Bassilian S, Bedi GS, Boontheung P, Cociorva D, Delahunty CM, Denny T, Dunsmore J, Faull KF, Gilligan J, Gonzalez-Begne M, Halgand F, Hall SC, …
• A "tagless" strategy for identification of stable protein complexes genome-wide by multidimensional orthogonal chromatographic separation and iTRAQ reagent tracking.
  Journal of proteome research 2008 Dong M, Yang LL, Williams K, Fisher SJ, Hall SC, Biggin MD, Jin J, Witkowska HE
• A mass spectrometry-based strategy for detecting and characterizing endogenous proteinase activities in complex biological samples.
  Proteomics 2008 Robinson S, Niles RK, Witkowska HE, Rittenbach KJ, Nichols RJ, Sargent JA, Dixon SE, Prakobphol A, Hall SC, Fisher SJ, Hardt M
• J Dent Res
  Reduced amelogenin-MMP20 interactions in amelogenesis imperfecta 2008 Tanimoto K, Le T, Zhu L, Witkowska HE, Robinson S, Hall S, Hwang P, Denbesten P, and Li Wu
• Natural Product Communications
  Analysis of saponin mixtures from Alfaalfa (Medicago sativa L.) roots using mass spectrometry with Maldi techniques 2008 Witkowska HE, Bialy Z, Jurzysta M, and Waller GR
• Apolipoprotein E*dipalmitoylphosphatidylcholine particles are ellipsoidal in solution.
  Journal of lipid research 2007 Peters-Libeu CA, Newhouse Y, Hall SC, Witkowska HE, Weisgraber KH
• Octomeric pyruvate-ferredoxin oxidoreductase from Desulfovibrio vulgaris.
  Journal of structural biology 2007 Garczarek F, Dong M, Typke D, Witkowska HE, Hazen TC, Nogales E, Biggin MD, Glaeser RM
• High-molecular-mass APOBEC3G complexes restrict Alu retrotransposition.
  Proceedings of the National Academy of Sciences of the United States of America 2006 Chiu YL, Witkowska HE, Hall SC, Santiago M, Soros VB, Esnault C, Heidmann T, Greene WC
• Assessing the effects of diurnal variation on the composition of human parotid saliva: quantitative analysis of native peptides using iTRAQ reagents.
  Analytical chemistry 2005 Hardt M, Witkowska HE, Webb S, Thomas LR, Dixon SE, Hall SC, Fisher SJ
• Toward defining the human parotid gland salivary proteome and peptidome: identification and characterization using 2D SDS-PAGE, ultrafiltration, HPLC, and mass spectrometry.
  Biochemistry 2005 Hardt M, Thomas LR, Dixon SE, Newport G, Agabian N, Prakobphol A, Hall SC, Witkowska HE, Fisher SJ
• Head-to-head coiled arrangement of the subunits of the animal fatty acid synthase.
  Chemistry & biology 2004 Witkowski A, Ghosal A, Joshi AK, Witkowska HE, Asturias FJ, Smith S
• Characterization of embryonic globin genes of the zebrafish.
  Developmental biology 2003 Brownlie A, Hersey C, Oates AC, Paw BH, Falick AM, Witkowska HE, Flint J, Higgs D, Jessen J, Bahary N, Zhu H, Lin S, Zon L
• J Chromatogr B Biomed Sci Appl
  Characterization of glycated hemoglobin in diabetic patients: usefulness of electrospray mass spectrometry in monitoring the extent and distribution of glycation 2001 Zhang X, Medzihradszky KF, Cunningham J, Lee PD, Rognerud CL, Ou CN, Harmatz P, and Witkowska HE
• Blood
  Fetal expression of a human Agamma globin transgene rescues globin chain imbalance but not hemolysis in EKLF null mouse embryos. 2000 Perkins AC, Peterson KR, Stamatoyannopoulos G, Witkowska HE, and Orkin SH
• J Biol Chem
  Dibromopropanone cross-linking of the phosphopantetheine and active-site cysteine thiols of the animal fatty acid synthase can occur both inter- and intrasubunit. Reevaluation of the side-by-side, antiparallel subunit model. 1999 Witkowski A, Joshi AK, Rangan VS, Falick AM, Witkowska HE, and Smith S.
• Nat Genet
  Positional cloning of the zebrafish sauternes gene: a model for congenital sideroblastic anaemia. 1998 Brownlie A, Donovan A, Pratt SJ, Paw BH, Oates AC, Brugnara C, Witkowska HE, Sassa S, and Zon LI.
• Blood
  Characterization of adult alpha- and beta-globin genes in the zebrafish. 1997 Chan FY, Robinson J, Brownlie A, Shivdasani RA, Donovan A, Brugnara C, Kim J, Lau BC, Witkowska HE, and Zon LI.
• Blood
  The human beta globin locus introduced by YAC transfer exhibits a specific and reproducible pattern of developmental regulation in transgenic mice 1997 Porcu S, Kitamura M, Witkowska E, Zhang Z, Mutero A, Lin C, Chang J, and Gaensler KM
• Br J Haematol
  HbC compound heterozygotes [HbC/Hb Riyadh and HbC/Hb N-Baltimore] with opposing effects upon HbC crystallization 1997 Hirsch RE, Witkowska HE, Shafer F, Lin MJ, Balazs TC, Bookchin RM, and Nagel RL.
• Science
  Transgenic knockout mice with exclusively human sickle hemoglobin and sickle cell disease 1997 Paszty C, Brion CM, Manci E, Witkowska HE, Stevens ME, Mohandas N, and Rubin EM.
• Steroids
  Characterization of bacterially expressed rat estrogen receptor beta ligand binding domain by mass spectrometry: structural comparison with estrogen receptor alpha 1997 Witkowska HE, Carlquist M, Engstrom O, Carlsson B, Bonn T, Gustafsson JA, and Shackleton CH.
• Anal Chem
  Characterizing abnormal hemoglobin by MS 1996 Shackleton CH, and Witkowska HE.
• J Biol Chem
  Expression of soluble human beta-globin chains in bacteria and assembly in vitro with alpha-globin chains 1996 Yamaguchi T, Pang J, Reddy KS, Witkowska HE, Surrey S, and Adachi K.
• J Am Chem Soc,
  Mass spectrometric analysis of a native zinc finger structure: the glucocorticoid receptor DNA binding domain. 1995 Witkowska HE,Shackleton CHL, Dahlman-Wright K, Kim JY, Gustaffson JA. (1995)
• Rapid Commun Mass Spectrom
  Intact protein electrospray ionization tandem mass spectrometry can be the sole technique used for confirming the structure of a variant hemoglobin 1995 Witkowska HE, Green BN, Morris M, and Shackleton CH
• Biochemistry
  Incorporation of norleucine at methionine positions in recombinant human macrophage colony stimulating factor (M-CSF, 4-153) expressed in Escherichia coli: structural analysis 1994 Randhawa ZI, Witkowska HE, Cone J, Wilkins JA, Hughes P, Yamanishi K, Yasuda S, Masui Y, Arthur P, Kletke C, and et al.
• Biol Mass Spectrom
  Collisionally activated dissociation and tandem mass spectrometry of intact hemoglobin beta-chain variant proteins with electrospray ionization 1993 Light-Wahl KJ, Loo JA, Edmonds CG, Smith RD, Witkowska HE, Shackleton CH, and Wu CS
• Hemoglobin
  Expediting rare variant hemoglobin characterization by combined HPLC/electrospray mass spectrometry 1993 Witkowska HE, Bitsch F, and Shackleton CH
• N Engl J Med
  A short-term trial of butyrate to stimulate fetal-globin-gene expression in the beta-globin disorders 1993 Perrine SP, Ginder GD, Faller DV, Dover GH, Ikuta T, Witkowska HE, Cai SP, Vichinsky EP, and Olivieri NF
• Protein Sci
  A study of intermediates involved in the folding pathway for recombinant human macrophage colony-stimulating factor (M-CSF): evidence for two distinct folding pathways 1993 Wilkins JA, Cone J, Randhawa ZI, Wood D, Warren MK, and Witkowska HE
• J Clin Invest
  Hypoxia-induced in vivo sickling of transgenic mouse red cells 1991 Rubin EM, Witkowska HE, Spangler E, Curtin P, Lubin BH, Mohandas N, and Clift SM
• N Engl J Med
  Sickle cell disease in a patient with sickle cell trait and compound heterozygosity for hemoglobin S and hemoglobin Quebec-Chori 1991 Witkowska HE, Lubin BH, Beuzard Y, Baruchel S, Esseltine DW, Vichinsky EP, Kleman KM, Bardakdjian-Michau J, Pinkoski L, Cahn S, and et al
• J Biol Chem
  The carboxyl-terminal region of thioesterase II participates in the interaction with fatty acid synthase. Use of electrospray ionization mass spectrometry to identify a carboxyl-terminally truncated form of the enzyme 1990 Witkowska HE, Green BN, and Smith S