Gary Cecchini, PhD
Employee of Affiliated Org.
Biochemistry and Biophysics
School of Medicine

Grants and Projects

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• BLR&D Research Career Scientist Award Application, NIH, 2018-2025
• Structure/Function of Complex II Oxidoreductase, NIH, 2001-2022
• The Role of Acetylation in Mitochondrial Function, NIH, 2011-2015
• Regulation of NADH: ubiquinone oxidoreductase (complex *, NIH, 2002-2008
• Molecular &Cellular Bioenergetics Gordon Conference, NIH, 2004-2005
• Membrane Bound Enzymes in Heart and Other Tissues, NIH, 1978-2002

Publications (70)

Top publication keywords:
HemeEscherichia coli ProteinsFlavoproteinsOxidoreductasesHydrogen SulfideElectron Transport Complex IEscherichia coliFumaratesElectron TransportFlavin-Adenine DinucleotideElectron Transport Complex IISuccinate DehydrogenaseNADUbiquinoneSulfides

• CryoEM structures reveal how the bacterial flagellum rotates and switches direction.
  Nature microbiology 2024 Singh PK, Sharma P, Afanzar O, Goldfarb MH, Maklashina E, Eisenbach M, Cecchini G, Iverson TM
• Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners.
  Nature communications 2024 Sharma P, Maklashina E, Voehler M, Balintova S, Dvorakova S, Kraus M, Hadrava Vanova K, Nahacka Z, Zobalova R, Boukalova S, Cunatova K, Mracek T, Ghayee HK, Pacak K, Rohlena J, Neuzil J, Cecchini G, …
• CryoEM structure of a post-assembly MS-ring reveals plasticity in stoichiometry and conformation.
  PloS one 2023 Singh PK, Cecchini G, Nakagawa T, Iverson TM
• An evolving view of complex II-noncanonical complexes, megacomplexes, respiration, signaling, and beyond.
  The Journal of biological chemistry 2023 Iverson TM, Singh PK, Cecchini G
• How an assembly factor enhances covalent FAD attachment to the flavoprotein subunit of complex II.
  The Journal of biological chemistry 2022 Maklashina E, Iverson TM, Cecchini G

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• Complexities of complex II: Sulfide metabolism in vivo.
  The Journal of biological chemistry 2022 Cecchini G
• The roles of SDHAF2 and dicarboxylate in covalent flavinylation of SDHA, the human complex II flavoprotein.
  Proceedings of the National Academy of Sciences of the United States of America 2020 Sharma P, Maklashina E, Cecchini G, Iverson TM
• Determination of Flavin Potential in Proteins by Xanthine/Xanthine Oxidase Method.
  Bio-protocol 2020 Maklashina E, Cecchini G
• A Mechanism of Modulating the Direction of Flagellar Rotation in Bacteria by Fumarate and Fumarate Reductase.
  Journal of molecular biology 2019 Koganitsky A, Tworowski D, Dadosh T, Cecchini G, Eisenbach M
• Maturation of the respiratory complex II flavoprotein.
  Current opinion in structural biology 2019 Sharma P, Maklashina E, Cecchini G, Iverson TM
• The unassembled flavoprotein subunits of human and bacterial complex II have impaired catalytic activity and generate only minor amounts of ROS.
  The Journal of biological chemistry 2018 Maklashina E, Rajagukguk S, Iverson TM, Cecchini G
• Crystal structure of an assembly intermediate of respiratory Complex II.
  Nature communications 2018 Sharma P, Maklashina E, Cecchini G, Iverson TM
• New crystal forms of the integral membrane Escherichia coli quinol:fumarate reductase suggest that ligands control domain movement.
  Journal of structural biology 2017 Starbird CA, Tomasiak TM, Singh PK, Yankovskaya V, Maklashina E, Eisenbach M, Cecchini G, Iverson TM
• Structural and biochemical analyses reveal insights into covalent flavinylation of the Escherichia coli Complex II homolog quinol:fumarate reductase.
  The Journal of biological chemistry 2017 Starbird CA, Maklashina E, Sharma P, Qualls-Histed S, Cecchini G, Iverson TM
• Binding of the Covalent Flavin Assembly Factor to the Flavoprotein Subunit of Complex II.
  The Journal of biological chemistry 2015 Maklashina E, Rajagukguk S, Starbird CA, McDonald WH, Koganitsky A, Eisenbach M, Iverson TM, Cecchini G
• Redox state of flavin adenine dinucleotide drives substrate binding and product release in Escherichia coli succinate dehydrogenase.
  Biochemistry 2015 Cheng VW, Piragasam RS, Rothery RA, Maklashina E, Cecchini G, Weiner JH
• Investigating the thermostability of succinate: quinone oxidoreductase enzymes by direct electrochemistry at SWNTs-modified electrodes and FTIR spectroscopy.
  Chemphyschem : a European journal of chemical physics and physical chemistry 2014 Melin F, Noor MR, Pardieu E, Boulmedais F, Banhart F, Cecchini G, Soulimane T, Hellwig P
• Electron-transfer pathways in the heme and quinone-binding domain of complex II (succinate dehydrogenase).
  Biochemistry 2014 Anderson RF, Shinde SS, Hille R, Rothery RA, Weiner JH, Rajagukguk S, Maklashina E, Cecchini G
• Plasticity of the quinone-binding site of the complex II homolog quinol:fumarate reductase.
  The Journal of biological chemistry 2013 Singh PK, Sarwar M, Maklashina E, Kotlyar V, Rajagukguk S, Tomasiak TM, Cecchini G, Iverson TM
• A conserved lysine residue controls iron-sulfur cluster redox chemistry in Escherichia coli fumarate reductase.
  Biochimica et biophysica acta 2013 Cheng VW, Tran QM, Boroumand N, Rothery RA, Maklashina E, Cecchini G, Weiner JH
• Respiratory complex II: role in cellular physiology and disease.
  Biochimica et biophysica acta 2013 Cecchini G
• Defining a direction: electron transfer and catalysis in Escherichia coli complex II enzymes.
  Biochimica et biophysica acta 2013 Maklashina E, Cecchini G, Dikanov SA
• Structural basis for malfunction in complex II.
  The Journal of biological chemistry 2012 Iverson TM, Maklashina E, Cecchini G
• CD133 is a marker for long-term repopulating murine epidermal stem cells.
  The Journal of investigative dermatology 2012 Charruyer A, Strachan LR, Yue L, Toth AS, Cecchini G, Mancianti ML, Ghadially R
• A novel intracellular isoform of matrix metalloproteinase-2 induced by oxidative stress activates innate immunity.
  PloS one 2012 Lovett DH, Mahimkar R, Raffai RL, Cape L, Maklashina E, Cecchini G, Karliner JS
• Out of plane distortions of the heme b of Escherichia coli succinate dehydrogenase.
  PloS one 2012 Tran QM, Fong C, Rothery RA, Maklashina E, Cecchini G, Weiner JH
• Energy complexes are apparently associated with the switch-motor complex of bacterial flagella.
  Journal of molecular biology 2011 Zarbiv G, Li H, Wolf A, Cecchini G, Caplan SR, Sourjik V, Eisenbach M
• A structural model for binding of the serine-rich repeat adhesin GspB to host carbohydrate receptors.
  PLoS pathogens 2011 Pyburn TM, Bensing BA, Xiong YQ, Melancon BJ, Tomasiak TM, Ward NJ, Yankovskaya V, Oliver KM, Cecchini G, Sulikowski GA, Tyska MJ, Sullam PM, Iverson TM
• Perturbation of the quinone-binding site of complex II alters the electronic properties of the proximal [3Fe-4S] iron-sulfur cluster.
  The Journal of biological chemistry 2011 Ruprecht J, Iwata S, Rothery RA, Weiner JH, Maklashina E, Cecchini G
• Molecular identification of the enzyme responsible for the mitochondrial NADH-supported ammonium-dependent hydrogen peroxide production.
  FEBS letters 2010 Kareyeva AV, Grivennikova VG, Cecchini G, Vinogradov AD
• Geometric restraint drives on- and off-pathway catalysis by the Escherichia coli menaquinol:fumarate reductase.
  The Journal of biological chemistry 2010 Tomasiak TM, Archuleta TL, Andréll J, Luna-Chávez C, Davis TA, Sarwar M, Ham AJ, McDonald WH, Yankovskaya V, Stern HA, Johnston JN, Maklashina E, Cecchini G, Iverson TM
• Purification, crystallization and preliminary X-ray diffraction analysis of the carbohydrate-binding region of the Streptococcus gordonii adhesin GspB.
  Acta crystallographica. Section F, Structural biology and crystallization communications 2010 Pyburn TM, Yankovskaya V, Bensing BA, Cecchini G, Sullam PM, Iverson TM
• The quinone-binding and catalytic site of complex II.
  Biochimica et biophysica acta 2010 Maklashina E, Cecchini G
• Mutation of the heme axial ligand of Escherichia coli succinate-quinone reductase: implications for heme ligation in mitochondrial complex II from yeast.
  Biochimica et biophysica acta 2010 Maklashina E, Rajagukguk S, McIntire WS, Cecchini G
• Cardioprotective activity of a novel and potent competitive inhibitor of lactate dehydrogenase.
  FEBS letters 2010 Kotlyar AB, Randazzo A, Honbo N, Jin ZQ, Karliner JS, Cecchini G
• Structure of Escherichia coli succinate:quinone oxidoreductase with an occupied and empty quinone-binding site.
  The Journal of biological chemistry 2009 Ruprecht J, Yankovskaya V, Maklashina E, Iwata S, Cecchini G
• Assembly of a chimeric respiratory chain from bovine heart submitochondrial particles and cytochrome bd terminal oxidase of Escherichia coli.
  FEBS letters 2009 Gavrikova EV, Grivennikova VG, Borisov VB, Cecchini G, Vinogradov AD
• Ammonium-dependent hydrogen peroxide production by mitochondria.
  FEBS letters 2008 Grivennikova VG, Cecchini G, Vinogradov AD
• A threonine on the active site loop controls transition state formation in Escherichia coli respiratory complex II.
  The Journal of biological chemistry 2008 Tomasiak TM, Maklashina E, Cecchini G, Iverson TM
• The bacterial flagellar switch complex is getting more complex.
  The EMBO journal 2008 Cohen-Ben-Lulu GN, Francis NR, Shimoni E, Noy D, Davidov Y, Prasad K, Sagi Y, Cecchini G, Johnstone RM, Eisenbach M
• Reversible dissociation of flavin mononucleotide from the mammalian membrane-bound NADH: ubiquinone oxidoreductase (complex I).
  FEBS letters 2007 Gostimskaya IS, Grivennikova VG, Cecchini G, Vinogradov AD
• Escherichia coli succinate dehydrogenase variant lacking the heme b.
  Proceedings of the National Academy of Sciences of the United States of America 2007 Tran QM, Rothery RA, Maklashina E, Cecchini G, Weiner JH
• Redox-dependent change of nucleotide affinity to the active site of the mammalian complex I.
  Biochemistry 2007 Grivennikova VG, Kotlyar AB, Karliner JS, Cecchini G, Vinogradov AD
• Transgenic MMP-2 expression induces latent cardiac mitochondrial dysfunction.
  Biochemical and biophysical research communications 2007 Zhou HZ, Ma X, Gray MO, Zhu BQ, Nguyen AP, Baker AJ, Simonis U, Cecchini G, Lovett DH, Karliner JS
• Succinate as Donor; Fumarate as Acceptor.
  EcoSal Plus 2007 Tomasiak TM, Cecchini G, Iverson TM
• The quinone binding site in Escherichia coli succinate dehydrogenase is required for electron transfer to the heme b.
  The Journal of biological chemistry 2006 Tran QM, Rothery RA, Maklashina E, Cecchini G, Weiner JH
• Differences in protonation of ubiquinone and menaquinone in fumarate reductase from Escherichia coli.
  The Journal of biological chemistry 2006 Maklashina E, Hellwig P, Rothery RA, Kotlyar V, Sher Y, Weiner JH, Cecchini G
• Comparison of pyrroloquinoline quinone and/or metoprolol on myocardial infarct size and mitochondrial damage in a rat model of ischemia/reperfusion injury.
  Journal of cardiovascular pharmacology and therapeutics 2006 Zhu BQ, Simonis U, Cecchini G, Zhou HZ, Li L, Teerlink JR, Karliner JS
• Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I).
  Biochimica et biophysica acta 2006 Gostimskaya IS, Cecchini G, Vinogradov AD
• Poly(ADP-ribose) polymerase-1 hyperactivation and impairment of mitochondrial respiratory chain complex I function in reperfused mouse hearts.
  American journal of physiology. Heart and circulatory physiology 2006 Zhou HZ, Swanson RA, Simonis U, Ma X, Cecchini G, Gray MO
• Fumarate reductase and succinate oxidase activity of Escherichia coli complex II homologs are perturbed differently by mutation of the flavin binding domain.
  The Journal of biological chemistry 2006 Maklashina E, Iverson TM, Sher Y, Kotlyar V, Andréll J, Mirza O, Hudson JM, Armstrong FA, Rothery RA, Weiner JH, Cecchini G
• Structural and computational analysis of the quinone-binding site of complex II (succinate-ubiquinone oxidoreductase): a mechanism of electron transfer and proton conduction during ubiquinone reduction.
  The Journal of biological chemistry 2005 Horsefield R, Yankovskaya V, Sexton G, Whittingham W, Shiomi K, Omura S, Byrne B, Cecchini G, Iwata S
• A novel strong competitive inhibitor of complex I.
  FEBS letters 2005 Kotlyar AB, Karliner JS, Cecchini G
• Electron transfer within complex II. Succinate:ubiquinone oxidoreductase of Escherichia coli.
  The Journal of biological chemistry 2005 Anderson RF, Hille R, Shinde SS, Cecchini G
• Electron transfer and catalytic control by the iron-sulfur clusters in a respiratory enzyme, E. coli fumarate reductase.
  Journal of the American Chemical Society 2005 Hudson JM, Heffron K, Kotlyar V, Sher Y, Maklashina E, Cecchini G, Armstrong FA
• Inhibitory effect of palmitate on the mitochondrial NADH:ubiquinone oxidoreductase (complex I) as related to the active-de-active enzyme transition.
  The Biochemical journal 2005 Loskovich MV, Grivennikova VG, Cecchini G, Vinogradov AD
• MnSOD in mouse heart: acute responses to ischemic preconditioning and ischemia-reperfusion injury.
  American journal of physiology. Heart and circulatory physiology 2005 Jin ZQ, Zhou HZ, Cecchini G, Gray MO, Karliner JS
• Defining the Q-site of Escherichia coli fumarate reductase by site-directed mutagenesis, fluorescence quench titrations and EPR spectroscopy.
  The FEBS journal 2005 Rothery RA, Seime AM, Spiers AM, Maklashina E, Schröder I, Gunsalus RP, Cecchini G, Weiner JH
• Absence of NADH channeling in coupled reaction of mitochondrial malate dehydrogenase and complex I in alamethicin-permeabilized rat liver mitochondria.
  Biochemical and biophysical research communications 2004 Kotlyar AB, Maklashina E, Cecchini G
• Effect of oxygen on activation state of complex I and lack of oxaloacetate inhibition of complex II in Langendorff perfused rat heart.
  FEBS letters 2004 Maklashina E, Kotlyar AB, Karliner JS, Cecchini G
• Active/de-active transition of respiratory complex I in bacteria, fungi, and animals.
  Biochimica et biophysica acta 2003 Maklashina E, Kotlyar AB, Cecchini G
• Variation in proton donor/acceptor pathways in succinate:quinone oxidoreductases.
  FEBS letters 2003 Cecchini G, Maklashina E, Yankovskaya V, Iverson TM, Iwata S
• Using rational screening and electron microscopy to optimize the crystallization of succinate:ubiquinone oxidoreductase from Escherichia coli.
  Acta crystallographica. Section D, Biological crystallography 2003 Horsefield R, Yankovskaya V, Törnroth S, Luna-Chavez C, Stambouli E, Barber J, Byrne B, Cecchini G, Iwata S
• Architecture of succinate dehydrogenase and reactive oxygen species generation.
  Science (New York, N.Y.) 2003 Yankovskaya V, Horsefield R, Törnroth S, Luna-Chavez C, Miyoshi H, Léger C, Byrne B, Cecchini G, Iwata S
• Function and structure of complex II of the respiratory chain.
  Annual review of biochemistry 2003 Cecchini G
• Effect of anoxia/reperfusion on the reversible active/de-active transition of NADH-ubiquinone oxidoreductase (complex I) in rat heart.
  Biochimica et biophysica acta 2002 Maklashina E, Sher Y, Zhou HZ, Gray MO, Karliner JS, Cecchini G
• Electron-transfer mechanisms through biological redox chains in multicenter enzymes.
  Journal of the American Chemical Society 2002 Jeuken LJ, Jones AK, Chapman SK, Cecchini G, Armstrong FA
• Crystallographic studies of the Escherichia coli quinol-fumarate reductase with inhibitors bound to the quinol-binding site.
  The Journal of biological chemistry 2002 Iverson TM, Luna-Chavez C, Croal LR, Cecchini G, Rees DC
• Purification, crystallisation and preliminary crystallographic studies of succinate:ubiquinone oxidoreductase from Escherichia coli.
  Biochimica et biophysica acta 2002 Törnroth S, Yankovskaya V, Cecchini G, Iwata S
• Succinate dehydrogenase and fumarate reductase from Escherichia coli.
  Biochimica et biophysica acta 2002 Cecchini G, Schröder I, Gunsalus RP, Maklashina E