Diane Barber, PhD
Professor
Cell and Tissue Biology
School of Dentistry

Education & Training

Show all (1) Hide

• Diversity, Equity, and Inclusion Champion Training University of California 02/2021

Websites

Show all (2) Hide

• Lab website (dentistry.ucsf.edu)
• Profile at UCSF Cancer Center (cancer.ucsf.edu)

Grants and Projects

Show all (11) Hide

• Roles for Intracellular pH Dynamics in Cancer, NIH, 2016-2022
• Regulation of Epithelial Plasticity, NIH, 2015-2019
• Retention of somatic mutations in cancers by changes in pH sensing, NIH, 2013-2015
• Mechanisms of Receptor Regulated Na+-H+ Exchange, NIH, 1993-2015
• Actions of the Sodium-Hydrogen Exchanger Subtype, NHE1, NIH, 1999-2013
• Mechanotransduction in Fibroblast, NIH, 2007-2011
• Signaling Mechanisms of Somatostatin Receptors, NIH, 1987-2002
• Mechanisms Regulating Intestinal Neurotensin Expression, NIH, 1989-1993
• Regulation of Neurotensin-containing Enteric Cells, NIH, 1987-1993
• Vip Release from Enteric Neurons in Culture, NIH, 1986-1900
• Vip Release from Enteric Neurons in Culture, NIH, 1985-1900

Publications (107)

Top publication keywords:
Focal AdhesionsActin-Related Protein 2-3 ComplexReceptors, ErythropoietinHistidineSodium-Hydrogen ExchangersActin CytoskeletonHydrogen-Ion ConcentrationSodium-Hydrogen Exchanger 1FibroblastsActin-Related Protein 2ProtonsCation Transport ProteinsDictyosteliumActinsMicrofilament Proteins

• TCR signaling promotes formation of an STS1-Cbl-b complex with pH-sensitive phosphatase activity that suppresses T cell function in acidic environments.
  Immunity 2023 Tsai YL, Arias-Badia M, Kadlecek TA, Lwin YM, Srinath A, Shah NH, Wang ZE, Barber D, Kuriyan J, Fong L, Weiss A
• Intracellular pH dynamics regulates intestinal stem cell lineage specification.
  Nature communications 2023 Liu Y, Reyes E, Castillo-Azofeifa D, Klein OD, Nystul T, Barber DL
• Arp2/3 complex activity is necessary for mouse ESC differentiation, times formative pluripotency, and enables lineage specification.
  Stem cell reports 2022 Aloisio FM, Barber DL
• Low pH Facilitates Heterodimerization of Mutant Isocitrate Dehydrogenase IDH1-R132H and Promotes Production of 2-Hydroxyglutarate.
  Biochemistry 2021 Sesanto R, Kuehn JF, Barber DL, White KA
• Ethyl isopropyl amiloride decreases oxidative phosphorylation and increases mitochondrial fusion in clonal untransformed and cancer cells.
  American journal of physiology. Cell physiology 2021 Manoli SS, Kisor K, Webb BA, Barber DL

Show all (102 more) Hide

• pHLARE: a new biosensor reveals decreased lysosome pH in cancer cells.
  Molecular biology of the cell 2020 Webb BA, Aloisio FM, Charafeddine RA, Cook J, Wittmann T, Barber DL
• An acidic residue buried in the dimer interface of isocitrate dehydrogenase 1 (IDH1) helps regulate catalysis and pH sensitivity.
  The Biochemical journal 2020 Luna LA, Lesecq Z, White KA, Hoang A, Scott DA, Zagnitko O, Bobkov AA, Barber DL, Schiffer JM, Isom DG, Sohl CD
• Intracellular pH Regulates Cancer and Stem Cell Behaviors: A Protein Dynamics Perspective.
  Frontiers in oncology 2020 Liu Y, White KA, Barber DL
• Editorial overview: Cell dynamics: Integrating cell dynamics across scales.
  Current opinion in cell biology 2020 Barber DL, Trepat X
• Filament assembly by the liver phosphofructokinase-1, the “gatekeeper” of glycolysis.
  The FASEB Journal 2020 Bradley Webb, Anne Dosey, Torsten Wittmann, Justin Kollmann, Diane Barber
• Intracellular pH dynamics and charge-changing somatic mutations in cancer.
  Cancer metastasis reviews 2019 White KA, Kisor K, Barber DL
• Drosophila anion exchanger 2 is required for proper ovary development and oogenesis.
  Developmental biology 2019 Benitez M, Tatapudy S, Liu Y, Barber DL, Nystul TG
• Tau repeat regions contain conserved histidine residues that modulate microtubule-binding in response to changes in pH.
  The Journal of biological chemistry 2019 Charafeddine RA, Cortopassi WA, Lak P, Tan R, McKenney RJ, Jacobson MP, Barber DL, Wittmann T
• β-Catenin is a pH sensor with decreased stability at higher intracellular pH.
  The Journal of cell biology 2018 White KA, Grillo-Hill BK, Esquivel M, Peralta J, Bui VN, Chire I, Barber DL
• Formin-dependent TGF-β signaling for epithelial to mesenchymal transition.
  Molecular biology of the cell 2018 Rana MK, Aloisio FM, Choi C, Barber DL
• The Glycolytic Enzyme Phosphofructokinase-1 Assembles into Filaments.
  Biophysical Journal 2018 Bradley Webb, Annie Dosey, Torsten Wittmann, Justin Kollman, Diane Barber
• Cell fate decisions: emerging roles for metabolic signals and cell morphology.
  EMBO reports 2017 Tatapudy S, Aloisio F, Barber D, Nystul T
• A Histidine pH sensor regulates activation of the Ras-specific guanine nucleotide exchange factor RasGRP1.
  eLife 2017 Vercoulen Y, Kondo Y, Iwig JS, Janssen AB, White KA, Amini M, Barber DL, Kuriyan J, Roose JP
• Cancer-associated arginine-to-histidine mutations confer a gain in pH sensing to mutant proteins.
  Science signaling 2017 White KA, Ruiz DG, Szpiech ZA, Strauli NB, Hernandez RD, Jacobson MP, Barber DL
• Prominent features of the amino acid mutation landscape in cancer.
  PloS one 2017 Szpiech ZA, Strauli NB, White KA, Ruiz DG, Jacobson MP, Barber DL, Hernandez RD
• The glycolytic enzyme phosphofructokinase-1 assembles into filaments.
  The Journal of cell biology 2017 Webb BA, Dosey AM, Wittmann T, Kollman JM, Barber DL
• Cancer cell behaviors mediated by dysregulated pH dynamics at a glance.
  Journal of cell science 2017 White KA, Grillo-Hill BK, Barber DL
• Increased intracellular pH is necessary for adult epithelial and embryonic stem cell differentiation.
  The Journal of cell biology 2016 Ulmschneider B, Grillo-Hill BK, Benitez M, Azimova DR, Barber DL, Nystul TG
• A Histidine Cluster in the Cytoplasmic Domain of the Na-H Exchanger NHE1 Confers pH-sensitive Phospholipid Binding and Regulates Transporter Activity.
  The Journal of biological chemistry 2016 Webb BA, White KA, Grillo-Hill BK, Schönichen A, Choi C, Barber DL
• Structures of Human Phosphofructokinase-1 and Atomic Basis of Cancer-Associated Mutations.
  Biophysical Journal 2016 Bradley Webb, Farhad Forouhar, Fu-En Szu, Jayaraman Seetharaman, Liang Tong, Diane Barber
• Abstract B65: Dissecting the tumor myeloid compartment reveals rare activating antigen presenting cells, critical for T cell immunity.
  Tumor Microenvironment 2015 Miranda Broz, Mikhail Binnewies, Bijan Boldajipour, Amanda Nelson, Joshua Pollock, David Erle, Andrea Barczak, Michael Rosenblum, Adil Daud, Diane Barber, Sebastian Amigorena, Laura J. van 't Veer, …
• Structures of human phosphofructokinase-1 and atomic basis of cancer-associated mutations.
  Nature 2015 Webb BA, Forouhar F, Szu FE, Seetharaman J, Tong L, Barber DL
• Increased H⁺ efflux is sufficient to induce dysplasia and necessary for viability with oncogene expression.
  eLife 2015 Grillo-Hill BK, Choi C, Jimenez-Vidal M, Barber DL
• Hypoxia increases the abundance but not the assembly of extracellular fibronectin during epithelial cell transdifferentiation.
  Journal of cell science 2015 Rana MK, Srivastava J, Yang M, Chen CS, Barber DL
• The Nck-interacting kinase NIK increases Arp2/3 complex activity by phosphorylating the Arp2 subunit.
  The Journal of cell biology 2015 LeClaire LL, Rana M, Baumgartner M, Barber DL
• Dissecting the Tumor Myeloid Compartment Reveals Rare Activating Antigen-Presenting Cells Critical for T Cell Immunity.
  Cancer cell 2014 Broz ML, Binnewies M, Boldajipour B, Nelson AE, Pollack JL, Erle DJ, Barczak A, Rosenblum MD, Daud A, Barber DL, Amigorena S, Van't Veer LJ, Sperling AI, Wolf DM, Krummel MF
• Dissecting the tumor myeloid compartment reveals rare activating antigen-presenting cells critical for T cell immunity.
  Cancer cell 2014 Broz ML, Binnewies M, Boldajipour B, Nelson AE, Pollack JL, Erle DJ, Barczak A, Rosenblum MD, Daud A, Barber DL, Amigorena S, Van't Veer LJ, Sperling AI, Wolf DM, Krummel MF
• Filament Assembly by Phosphofructokinase-1, the Gatekeeper of Glycolysis.
  Biophysical Journal 2014 Bradley Webb, Larry Ackerman, Diane Barber
• Ratiometric imaging of pH probes.
  Methods in cell biology 2014 Grillo-Hill BK, Webb BA, Barber DL
• pH Sensing by FAK-His58 Regulates Focal Adhesion Remodeling.
  The Journal of General Physiology 2013 Chang-Hoon Choi, B Webb, M Chimenti, M Jacobson, Diane L Barber
• pH sensing by FAK-His58 regulates focal adhesion remodeling.
  The Journal of cell biology 2013 Choi CH, Webb BA, Chimenti MS, Jacobson MP, Barber DL
• Electrostatics control actin filament nucleation and elongation kinetics.
  The Journal of biological chemistry 2013 Crevenna AH, Naredi-Rainer N, Schönichen A, Dzubiella J, Barber DL, Lamb DC, Wedlich-Söldner R
• Considering protonation as a posttranslational modification regulating protein structure and function.
  Annual review of biophysics 2013 Schönichen A, Webb BA, Jacobson MP, Barber DL
• Phosphorylation of actin-related protein 2 (Arp2) is required for normal development and cAMP chemotaxis in Dictyostelium.
  The Journal of biological chemistry 2012 Choi CH, Thomason PA, Zaki M, Insall RH, Barber DL
• Characterization of cytoskeletal protein 4.1R interaction with NHE1 (Na(+)/H(+) exchanger isoform 1).
  The Biochemical journal 2012 Nunomura W, Denker SP, Barber DL, Takakuwa Y, Gascard P
• Phosphorylation of the Arp2 subunit relieves auto-inhibitory interactions for Arp2/3 complex activation.
  PLoS computational biology 2011 Narayanan A, LeClaire LL, Barber DL, Jacobson MP
• Dynamic actin remodeling during epithelial-mesenchymal transition depends on increased moesin expression.
  Molecular biology of the cell 2011 Haynes J, Srivastava J, Madson N, Wittmann T, Barber DL
• Dysregulated pH: a perfect storm for cancer progression.
  Nature reviews. Cancer 2011 Webb BA, Chimenti M, Jacobson MP, Barber DL
• A human MAP kinase interactome.
  Nature methods 2010 Bandyopadhyay S, Chiang CY, Srivastava J, Gersten M, White S, Bell R, Kurschner C, Martin C, Smoot M, Sahasrabudhe S, Barber DL, Chanda SK, Ideker T
• Nuclear-localized calcineurin homologous protein CHP1 interacts with upstream binding factor and inhibits ribosomal RNA synthesis.
  The Journal of biological chemistry 2010 Jiménez-Vidal M, Srivastava J, Putney LK, Barber DL
• Expression of actin-interacting protein 1 suppresses impaired chemotaxis of Dictyostelium cells lacking the Na+-H+ exchanger NHE1.
  Molecular biology of the cell 2010 Choi CH, Patel H, Barber DL
• Molecular Dynamics Simulations Predict A pH-Dependent Conformational Change in the C-Helix of Cell Cycle Checkpoint Kinase Wee1.
  Biophysical Journal 2010 Michael S. Chimenti, Mark J.S. Kelly, Diane L. Barber, Matthew P. Jacobson
• The sodium-hydrogen exchanger NHE1 is an Akt substrate necessary for actin filament reorganization by growth factors.
  The Journal of biological chemistry 2009 Meima ME, Webb BA, Witkowska HE, Barber DL
• Mislocalized scaffolding by the Na-H exchanger NHE1 dominantly inhibits fibronectin production and TGF-beta activation.
  Molecular biology of the cell 2009 Karydis A, Jimenez-Vidal M, Denker SP, Barber DL
• Cofilin is a pH sensor for actin free barbed end formation: role of phosphoinositide binding.
  The Journal of cell biology 2008 Frantz C, Barreiro G, Dominguez L, Chen X, Eddy R, Condeelis J, Kelly MJ, Jacobson MP, Barber DL
• Structural model and functional significance of pH-dependent talin-actin binding for focal adhesion remodeling.
  Proceedings of the National Academy of Sciences of the United States of America 2008 Srivastava J, Barreiro G, Groscurth S, Gingras AR, Goult BT, Critchley DR, Kelly MJ, Jacobson MP, Barber DL
• Phosphorylation of the Arp2/3 complex is necessary to nucleate actin filaments.
  The Journal of cell biology 2008 LeClaire LL, Baumgartner M, Iwasa JH, Mullins RD, Barber DL
• Actin co-sedimentation assay; for the analysis of protein binding to F-actin.
  Journal of visualized experiments : JoVE 2008 Srivastava J, Barber D
• Positive feedback between Cdc42 activity and H+ efflux by the Na-H exchanger NHE1 for polarity of migrating cells.
  The Journal of cell biology 2007 Frantz C, Karydis A, Nalbant P, Hahn KM, Barber DL
• Beyond ion translocation: structural functions of the sodium-hydrogen exchanger isoform-1.
  Current opinion in nephrology and hypertension 2007 Meima ME, Mackley JR, Barber DL
• Intracellular pH sensors: design principles and functional significance.
  Physiology (Bethesda, Md.) 2007 Srivastava J, Barber DL, Jacobson MP
• The Nck-interacting kinase phosphorylates ERM proteins for formation of lamellipodium by growth factors.
  Proceedings of the National Academy of Sciences of the United States of America 2006 Baumgartner M, Sillman AL, Blackwood EM, Srivastava J, Madson N, Schilling JW, Wright JH, Barber DL
• A developmentally regulated Na-H exchanger in Dictyostelium discoideum is necessary for cell polarity during chemotaxis.
  The Journal of cell biology 2005 Patel H, Barber DL
• Na(+)/H(+) exchanger NHE1 as plasma membrane scaffold in the assembly of signaling complexes.
  American journal of physiology. Cell physiology 2004 Baumgartner M, Patel H, Barber DL
• Expression profile of genes regulated by activity of the Na-H exchanger NHE1.
  BMC genomics 2004 Putney LK, Barber DL
• Na-H exchange-dependent increase in intracellular pH times G2/M entry and transition.
  The Journal of biological chemistry 2003 Putney LK, Barber DL
• Conserved motifs in somatostatin, D2-dopamine, and alpha 2B-adrenergic receptors for inhibiting the Na-H exchanger, NHE1.
  The Journal of biological chemistry 2003 Lin CY, Varma MG, Joubel A, Madabushi S, Lichtarge O, Barber DL
• Cell migration requires both ion translocation and cytoskeletal anchoring by the Na-H exchanger NHE1.
  The Journal of cell biology 2002 Denker SP, Barber DL
• Ion transport proteins anchor and regulate the cytoskeleton.
  Current opinion in cell biology 2002 Denker SP, Barber DL
• The changing face of the Na+/H+ exchanger, NHE1: structure, regulation, and cellular actions.
  Annual review of pharmacology and toxicology 2002 Putney LK, Denker SP, Barber DL
• TEL-JAK2 mediates constitutive activation of the phosphatidylinositol 3'-kinase/protein kinase B signaling pathway.
  The Journal of biological chemistry 2001 Nguyen MH, Ho JM, Beattie BK, Barber DL
• The Nck-interacting kinase (NIK) phosphorylates the Na+-H+ exchanger NHE1 and regulates NHE1 activation by platelet-derived growth factor.
  The Journal of biological chemistry 2001 Yan W, Nehrke K, Choi J, Barber DL
• A common epitope is shared by activated signal transducer and activator of transcription-5 (STAT5) and the phosphorylated erythropoietin receptor: implications for the docking model of STAT activation.
  Blood 2001 Barber DL, Beattie BK, Mason JM, Nguyen MH, Yoakim M, Neel BG, D'Andrea AD, Frank DA
• A minimal cytoplasmic subdomain of the erythropoietin receptor mediates p70 S6 kinase phosphorylation.
  Experimental hematology 2001 Zhang MY, Barber DL, Alessi DR, Bell LL, Stine C, Nguyen MH, Beattie BK, Cheung JY, Miller BA
• Direct binding of the Na--H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation.
  Molecular cell 2000 Denker SP, Huang DC, Orlowski J, Furthmayr H, Barber DL
• The SH2 inositol 5-phosphatase Ship1 is recruited in an SH2-dependent manner to the erythropoietin receptor.
  The Journal of biological chemistry 2000 Mason JM, Beattie BK, Liu Q, Dumont DJ, Barber DL
• Effect of Rho GTPases on Na-H exchanger in mammalian cells.
  Methods in enzymology 2000 Denker SP, Yan W, Barber DL
• Molecular analysis of system N suggests novel physiological roles in nitrogen metabolism and synaptic transmission.
  Cell 1999 Chaudhry FA, Reimer RJ, Krizaj D, Barber D, Storm-Mathisen J, Copenhagen DR, Edwards RH
• Inhibition of calcineurin phosphatase activity by a calcineurin B homologous protein.
  The Journal of biological chemistry 1999 Lin X, Sikkink RA, Rusnak F, Barber DL
• Fusion of the ets transcription factor TEL to Jak2 results in constitutive Jak-Stat signaling.
  Blood 1999 Ho JM, Beattie BK, Squire JA, Frank DA, Barber DL
• p160ROCK mediates RhoA activation of Na-H exchange.
  The EMBO journal 1998 Tominaga T, Ishizaki T, Narumiya S, Barber DL
• Na-H exchange acts downstream of RhoA to regulate integrin-induced cell adhesion and spreading.
  Molecular biology of the cell 1998 Tominaga T, Barber DL
• Maintenance of human T cell anergy: blocking of IL-2 gene transcription by activated Rap1.
  Science (New York, N.Y.) 1997 Boussiotis VA, Freeman GJ, Berezovskaya A, Barber DL, Nadler LM
• Erythropoietin and interleukin-3 activate tyrosine phosphorylation of CBL and association with CRK adaptor proteins.
  Blood 1997 Barber DL, Mason JM, Fukazawa T, Reedquist KA, Druker BJ, Band H, D'Andrea AD
• Erythropoietin activates Raf1 by an Shc-independent pathway in CTLL-EPO-R cells.
  Blood 1997 Barber DL, Corless CN, Xia K, Roberts TM, D'Andrea AD
• Activation of Na+-H+ exchange is necessary for RhoA-induced stress fiber formation.
  Journal of Biological Chemistry 1996 Zinaida S. Vexler, Marc Symons, Diane L. Barber
• A calcineurin homologous protein inhibits GTPase-stimulated Na-H exchange.
  Proceedings of the National Academy of Sciences of the United States of America 1996 Lin X, Barber DL
• The cytokine-activated tyrosine kinase JAK2 activates Raf-1 in a p21ras-dependent manner.
  Proceedings of the National Academy of Sciences of the United States of America 1996 Xia K, Mukhopadhyay NK, Inhorn RC, Barber DL, Rose PE, Lee RS, Narsimhan RP, D'Andrea AD, Griffin JD, Roberts TM
• Activation of Na+-H+ exchange is necessary for RhoA-induced stress fiber formation.
  The Journal of biological chemistry 1996 Vexler ZS, Symons M, Barber DL
• Galpha12 differentially regulates Na+-H+ exchanger isoforms.
  The Journal of biological chemistry 1996 Lin X, Voyno-Yasenetskaya TA, Hooley R, Lin CY, Orlowski J, Barber DL
• A constitutively activated chimeric cytokine receptor confers factor-independent growth in hematopoietic cell lines.
  Blood 1996 Shikama Y, Barber DL, D'Andrea AD, Sieff CA
• G alpha 13 stimulates Na+-H+ exchange through distinct Cdc42-dependent and RhoA-dependent pathways.
  The Journal of biological chemistry 1996 Hooley R, Yu CY, Symons M, Barber DL
• [12] Regulation of Na+-H+ exchange by G protein-coupled receptors.
  Receptor Molecular Biology 1995 Kim A. Neve, Mary P. Rosser, Diane L. Barber
• The Fanconi anemia polypeptide FACC is localized to the cytoplasm.
  Proceedings of the National Academy of Sciences of the United States of America 1994 Yamashita T, Barber DL, Zhu Y, Wu N, D'Andrea AD
• Subtype-specific signaling mechanisms of somatostatin receptors SSTR1 and SSTR2.
  The Journal of biological chemistry 1994 Hou C, Gilbert RL, Barber DL
• A dominant negative erythropoietin (EPO) receptor inhibits EPO-dependent growth and blocks F-gp55-dependent transformation.
  Molecular and cellular biology 1994 Barber DL, DeMartino JC, Showers MO, D'Andrea AD
• G alpha 13 stimulates Na-H exchange.
  The Journal of biological chemistry 1994 Voyno-Yasenetskaya T, Conklin BR, Gilbert RL, Hooley R, Bourne HR, Barber DL
• Guanine nucleotides regulate beta-adrenergic activation of Na-H exchange independently of receptor coupling to Gs.
  The Journal of biological chemistry 1992 Barber DL, Ganz MB
• The erythropoietin receptor and the molecular basis of signal transduction.
  Seminars in hematology 1992 Barber DL, D'Andrea AD
• Mutant constructs of the beta-adrenergic receptor that are uncoupled from adenylyl cyclase retain functional activation of Na-H exchange.
  Molecular pharmacology 1992 Barber DL, Ganz MB, Bongiorno PB, Strader CD
• Fatty acids stereospecifically stimulate neurotensin release and increase [Ca2+]i in enteric endocrine cells.
  The American journal of physiology 1991 Barber DL, Cacace AM, Raucci DT, Ganz MB
• Mechanisms of receptor-mediated regulation of Na-H exchange.
  Cellular signalling 1991 Barber DL
• Multiple receptors coupled to adenylate cyclase regulate Na-H exchange independent of cAMP.
  The Journal of biological chemistry 1990 Ganz MB, Pachter JA, Barber DL
• Beta-adrenergic and somatostatin receptors regulate Na-H exchange independent of cAMP.
  The Journal of biological chemistry 1989 Barber DL, McGuire ME, Ganz MB
• Canine enteric submucosal cultures: transmitter release from neurotensin-immunoreactive neurons.
  Neuroscience 1989 Barber DL, Buchan AM, Leeman SE, Soll AH
• Somatostatin and muscarinic inhibition of canine enteric endocrine cells: cellular mechanisms.
  The American journal of physiology 1987 Barber DL, Gregor M, Soll AH
• Morphologic and physiologic studies of canine ileal enteroglucagon-containing cells in short-term culture.
  Gastroenterology 1987 Buchan AM, Barber DL, Gregor M, Soll AH
• Cloning and sequence analysis of cDNA for the canine neurotensin/neuromedin N precursor.
  Proceedings of the National Academy of Sciences of the United States of America 1987 Dobner PR, Barber DL, Villa-Komaroff L, McKiernan C
• Neurotensin-containing neurons in the canine enteric innervation.
  Neuroscience letters 1987 Buchan AM, Barber DL
• Chapter 9 Separation of Epithelial Cells from Gastric and Intestinal Mucosa.
  Cell Separation 1987 DIANE L. BARBER, ANDREW H. SOLL
• Isolated canine ileal mucosal cells in short-term culture: a model for study of neurotensin release.
  The American journal of physiology 1986 Barber DL, Buchan AM, Walsh JH, Soll AH
• Regulation of neurotensin release from canine enteric primary cell cultures.
  The American journal of physiology 1986 Barber DL, Buchan AM, Walsh JH, Soll AH