David Toczyski, PhD
Professor
HDF Comprehensive Cancer Ctr
School of Medicine

Websites

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• Profile at UCSF Cancer Center (cancer.ucsf.edu)

Grants and Projects

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• Regulation by post-translation modifications in response to stress, NIH, 2016-2026
• Identifying the targets of oncogenic/tumor-suppressive F box proteins, NIH, 2015-2018
• Regulation and Targets of the DNA Damage Checkpoint, NIH, 1999-2017
• Cell cycle regulation by ubiquitin ligases, NIH, 2004-2017
• Yeast Chromosome Structure, Replication and Segregation, NIH, 2006-2011

Publications (58)

Top publication keywords:
Ubiquitin-Protein LigasesCell Cycle ProteinsUbiquitinationMitosisSaccharomyces cerevisiaeCheckpoint Kinase 2DNA ReplicationAnaphase-Promoting Complex-CyclosomeSaccharomyces cerevisiae ProteinsPolyubiquitinUbiquitinDNA DamageCell CycleF-Box ProteinsUbiquitin-Protein Ligase Complexes

• The PRC2.1 Subcomplex Opposes G1 Progression through Regulation of CCND1 and CCND2.
  bioRxiv : the preprint server for biology 2024 Longhurst AD, Wang K, Suresh HG, Ketavarapu M, Ward HN, Jones IR, Narayan V, Hundley FV, Hassan AZ, Boone C, Myers CL, Shen Y, Ramani V, Andrews BJ, Toczyski DP
• Chemical-genetic CRISPR-Cas9 screens in human cells using a pathway-specific library.
  STAR protocols 2021 Hundley FV, Toczyski DP
• A comprehensive phenotypic CRISPR-Cas9 screen of the ubiquitin pathway uncovers roles of ubiquitin ligases in mitosis.
  Molecular cell 2021 Hundley FV, Sanvisens Delgado N, Marin HC, Carr KL, Tian R, Toczyski DP
• Fifty years of cycling.
  Molecular biology of the cell 2020 Biggins S, Hartwell L, Toczyski D
• Redundant targeting of Isr1 by two CDKs in mitotic cells.
  Current genetics 2020 Alme EB, Toczyski DP

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• The kinase Isr1 negatively regulates hexosamine biosynthesis in S. cerevisiae.
  PLoS genetics 2020 Alme EB, Stevenson E, Krogan NJ, Swaney DL, Toczyski DP
• Mck1 kinase is a new player in the DNA damage checkpoint pathway.
  PLoS genetics 2019 Sanvisens Delgado N, Toczyski DP
• A genetic approach to study polyubiquitination in Saccharomyces cerevisiae.
  Methods in enzymology 2019 Meza-Gutierrez F, Simsek D, Toczyski DP
• Genetic analysis reveals functions of atypical polyubiquitin chains.
  eLife 2018 Meza Gutierrez F, Simsek D, Mizrak A, Deutschbauer A, Braberg H, Johnson J, Xu J, Shales M, Nguyen M, Tamse-Kuehn R, Palm C, Steinmetz LM, Krogan NJ, Toczyski DP
• The Yeast DNA Damage Checkpoint Kinase Rad53 Targets the Exoribonuclease, Xrn1.
  G3 (Bethesda, Md.) 2018 Lao JP, Ulrich KM, Johnson JR, Newton BW, Vashisht AA, Wohlschlegel JA, Krogan NJ, Toczyski DP
• Shelterin and subtelomeric DNA sequences control nucleosome maintenance and genome stability.
  EMBO reports 2018 van Emden TS, Forn M, Forné I, Sarkadi Z, Capella M, Martín Caballero L, Fischer-Burkart S, Brönner C, Simonetta M, Toczyski D, Halic M, Imhof A, Braun S
• Phosphorylation and Proteasome Recognition of the mRNA-Binding Protein Cth2 Facilitates Yeast Adaptation to Iron Deficiency.
  mBio 2018 Romero AM, Martínez-Pastor M, Du G, Solé C, Carlos M, Vergara SV, Sanvisens N, Wohlschlegel JA, Toczyski DP, Posas F, de Nadal E, Martínez-Pastor MT, Thiele DJ, Puig S
• Isolation of ubiquitinated substrates by tandem affinity purification of E3 ligase-polyubiquitin-binding domain fusions (ligase traps).
  Nature protocols 2016 Mark KG, Loveless TB, Toczyski DP
• Parallel Parkin: Cdc20 Takes a New Partner.
  Molecular cell 2015 Meza-Gutierrez F, Hundley FV, Toczyski DP
• Prb1 Protease Activity Is Required for Its Recognition by the F-Box Protein Saf1.
  Biochemistry 2015 Mark KG, Meza-Gutierrez F, Johnson JR, Newton BW, Krogan NJ, Toczyski DP
• DNA Damage Regulates Translation through β-TRCP Targeting of CReP.
  PLoS genetics 2015 Loveless TB, Topacio BR, Vashisht AA, Galaang S, Ulrich KM, Young BD, Wohlschlegel JA, Toczyski DP
• Ndd1 turnover by SCF(Grr1) is inhibited by the DNA damage checkpoint in Saccharomyces cerevisiae.
  PLoS genetics 2015 Edenberg ER, Mark KG, Toczyski DP
• Acetylome profiling reveals overlap in the regulation of diverse processes by sirtuins, gcn5, and esa1.
  Molecular & cellular proteomics : MCP 2014 Downey M, Johnson JR, Davey NE, Newton BW, Johnson TL, Galaang S, Seller CA, Krogan N, Toczyski DP
• Polymerase stalling during replication, transcription and translation.
  Current biology : CB 2014 Edenberg ER, Downey M, Toczyski D
• Hst3 is turned over by a replication stress-responsive SCF(Cdc4) phospho-degron.
  Proceedings of the National Academy of Sciences of the United States of America 2014 Edenberg ER, Vashisht AA, Topacio BR, Wohlschlegel JA, Toczyski DP
• Ubiquitin ligase trapping identifies an SCF(Saf1) pathway targeting unprocessed vacuolar/lysosomal proteins.
  Molecular cell 2014 Mark KG, Simonetta M, Maiolica A, Seller CA, Toczyski DP
• Rad53 downregulates mitotic gene transcription by inhibiting the transcriptional activator Ndd1.
  Molecular and cellular biology 2013 Edenberg ER, Vashisht A, Benanti JA, Wohlschlegel J, Toczyski DP
• Gcn5 and sirtuins regulate acetylation of the ribosomal protein transcription factor Ifh1.
  Current biology : CB 2013 Downey M, Knight B, Vashisht AA, Seller CA, Wohlschlegel JA, Shore D, Toczyski DP
• F-box protein specificity for g1 cyclins is dictated by subcellular localization.
  PLoS genetics 2012 Landry BD, Doyle JP, Toczyski DP, Benanti JA
• Ubiquitin ligases: Taming the APC.
  Nature chemical biology 2012 Foe IT, Toczyski DP
• Keeping it together in times of stress: checkpoint function at stalled replication forks.
  Molecular cell 2012 Berens TJ, Toczyski DP
• Colocalization of Mec1 and Mrc1 is sufficient for Rad53 phosphorylation in vivo.
  Molecular biology of the cell 2012 Berens TJ, Toczyski DP
• Ubiquitination of Cdc20 by the APC occurs through an intramolecular mechanism.
  Current biology : CB 2011 Foe IT, Foster SA, Cheung SK, DeLuca SZ, Morgan DO, Toczyski DP
• Structural biology: a new look for the APC.
  Nature 2011 Foe I, Toczyski D
• Cross-species chemogenomic profiling reveals evolutionarily conserved drug mode of action.
  Molecular systems biology 2010 Kapitzky L, Beltrao P, Berens TJ, Gassner N, Zhou C, Wüster A, Wu J, Babu MM, Elledge SJ, Toczyski D, Lokey RS, Krogan NJ
• Cdc5 blocks in vivo Rad53 activity, but not in situ activity (ISA).
  Cell cycle (Georgetown, Tex.) 2010 Lopez-Mosqueda J, Vidanes GM, Toczyski DP
• Damage-induced phosphorylation of Sld3 is important to block late origin firing.
  Nature 2010 Lopez-Mosqueda J, Maas NL, Jonsson ZO, Defazio-Eli LG, Wohlschlegel J, Toczyski DP
• Repair scaffolding reaches new heights at blocked replication forks.
  Molecular cell 2010 Downey M, Edenberg ER, Toczyski DP
• CDC5 inhibits the hyperphosphorylation of the checkpoint kinase Rad53, leading to checkpoint adaptation.
  PLoS biology 2010 Vidanes GM, Sweeney FD, Galicia S, Cheung S, Doyle JP, Durocher D, Toczyski DP
• Functionally distinct isoforms of Cik1 are differentially regulated by APC/C-mediated proteolysis.
  Molecular cell 2009 Benanti JA, Matyskiela ME, Morgan DO, Toczyski DP
• Cdc20, an activator at last.
  Molecular cell 2008 Benanti JA, Toczyski DP
• Colocalization of sensors is sufficient to activate the DNA damage checkpoint in the absence of damage.
  Molecular cell 2008 Bonilla CY, Melo JA, Toczyski DP
• A proteomic screen reveals SCFGrr1 targets that regulate the glycolytic-gluconeogenic switch.
  Nature cell biology 2007 Benanti JA, Cheung SK, Brady MC, Toczyski DP
• Sensitivity of yeast strains with long G-tails to levels of telomere-bound telomerase.
  PLoS genetics 2007 Vega LR, Phillips JA, Thornton BR, Benanti JA, Onigbanjo MT, Toczyski DP, Zakian VA
• Functional dissection of protein complexes involved in yeast chromosome biology using a genetic interaction map.
  Nature 2007 Collins SR, Miller KM, Maas NL, Roguev A, Fillingham J, Chu CS, Schuldiner M, Gebbia M, Recht J, Shales M, Ding H, Xu H, Han J, Ingvarsdottir K, Cheng B, Andrews B, Boone C, Berger SL, Hieter P, Zhang…
• Precise destruction: an emerging picture of the APC.
  Genes & development 2006 Thornton BR, Toczyski DP
• Taking it off: regulation of H3 K56 acetylation by Hst3 and Hst4.
  Cell cycle (Georgetown, Tex.) 2006 Miller KM, Maas NL, Toczyski DP
• Cell cycle and checkpoint regulation of histone H3 K56 acetylation by Hst3 and Hst4.
  Molecular cell 2006 Maas NL, Miller KM, DeFazio LG, Toczyski DP
• An architectural map of the anaphase-promoting complex.
  Genes & development 2006 Thornton BR, Ng TM, Matyskiela ME, Carroll CW, Morgan DO, Toczyski DP
• Methods for studying adaptation to the DNA damage checkpoint in yeast.
  Methods in enzymology 2006 Toczyski DP
• Complicated tails: histone modifications and the DNA damage response.
  Cell 2005 Vidanes GM, Bonilla CY, Toczyski DP
• Damage in transition.
  Trends in biochemical sciences 2005 Garber PM, Vidanes GM, Toczyski DP
• DNA breaks promote genomic instability by impeding proper chromosome segregation.
  Current biology : CB 2004 Kaye JA, Melo JA, Cheung SK, Vaze MB, Haber JE, Toczyski DP
• Cycling without the cyclosome: modeling a yeast strain lacking the APC.
  Cell cycle (Georgetown, Tex.) 2004 Thornton BR, Chen KC, Cross FR, Tyson JJ, Toczyski DP
• Securin and B-cyclin/CDK are the only essential targets of the APC.
  Nature cell biology 2003 Thornton BR, Toczyski DP
• A unified view of the DNA-damage checkpoint.
  Current opinion in cell biology 2002 Melo J, Toczyski D
• Two checkpoint complexes are independently recruited to sites of DNA damage in vivo.
  Genes & development 2001 Melo JA, Cohen J, Toczyski DP
• Checkpoint adaptation precedes spontaneous and damage-induced genomic instability in yeast.
  Molecular and cellular biology 2001 Galgoczy DJ, Toczyski DP
• CDC5 and CKII control adaptation to the yeast DNA damage checkpoint.
  Cell 1997 Toczyski DP, Galgoczy DJ, Hartwell LH
• When checkpoints fail.
  Cell 1997 Paulovich AG, Toczyski DP, Hartwell LH
• The Epstein-Barr virus (EBV) small RNA EBER1 binds and relocalizes ribosomal protein L22 in EBV-infected human B lymphocytes.
  Proceedings of the National Academy of Sciences of the United States of America 1994 Toczyski DP, Matera AG, Ward DC, Steitz JA
• The cellular RNA-binding protein EAP recognizes a conserved stem-loop in the Epstein-Barr virus small RNA EBER 1.
  Molecular and cellular biology 1993 Toczyski DP, Steitz JA
• EAP, a highly conserved cellular protein associated with Epstein-Barr virus small RNAs (EBERs).
  The EMBO journal 1991 Toczyski DP, Steitz JA