David Nguyen, MD, PhD
Assistant Professor
Medicine
School of Medicine

Robert Langer with applications in DNA vaccines and immunostimulatory RNA vaccine adjuvants. He then went to Stanford University for medical school, and since 2012 has been at the UCSF Medical Center where he completed medical residency and a clinical fellowship in Infectious Diseases, and a postdoc fellowship in Alex Marson's laboratory developing technologies for CRISPR gene editing in immune cells.

David is currently an Assistant Professor of Medicine at the University of California San Francisco, Department of Medicine & Division of Infectious Diseases. His laboratory is based at the University of California, Berkeley Innovative Genomics Institute (IGI). The Nguyen lab focuses on three unmet needs for helping patients with primary immunodeficiency (PID) diseases: (i) genome engineering in primary human cells to identify and diagnose patient-specific PID mutations, (ii) functional CRISPR screens to understand and predict how PID mutations impact immune cell behavior, and (iii) developing novel gene editing technologies for correcting pathogenic mutations in the immune and blood (hematopoietic lineage) system – including PIDs and hemoglobinopathies. David is also an attending physician at UCSF with the adult infectious diseases service and immunology clinic, where he specializes in preventing and treating infections in patients with complex genetic immune defects.

• Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic stem cells.
  bioRxiv : the preprint server for biology 2024 Sahu S, Castro M, Muldoon JJ, Asija K, Wyman SK, Krishnappa N, de Onate L, Eyquem J, Nguyen DN, Wilson RC
• NKp30 and NKG2D contribute to natural killer recognition of HIV-infected cells.
  bioRxiv : the preprint server for biology 2024 Zhao NQ, Pi R, Nguyen DN, Ranganath T, Seiler C, Holmes S, Marson A, Blish CA
• Transition of care in inborn errors of immunity: Outcomes of a single-center quality improvement initiative.
  The journal of allergy and clinical immunology. In practice 2023 Sanchez LA, Tang M, Ahmed A, Nussbaum JC, Nguyen DN, Muskat M, Chen XH, Pham MN
• Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes.
  Nature biomedical engineering 2023 Foss DV, Muldoon JJ, Nguyen DN, Carr D, Sahu SU, Hunsinger JM, Wyman SK, Krishnappa N, Mendonsa R, Schanzer EV, Shy BR, Vykunta VS, Allain V, Li Z, Marson A, Eyquem J, Wilson RC
• High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails.
  Nature biotechnology 2022 Shy BR, Vykunta VS, Ha A, Talbot A, Roth TL, Nguyen DN, Pfeifer WG, Chen YY, Blaeschke F, Shifrut E, Vedova S, Mamedov MR, Chung JJ, Li H, Yu R, Wu D, Wolf J, Martin TG, Castro CE, Ye L, Esensten JH, …
• Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturing
  BioRxiv 2021 Shy B, Vykunta V, Ha A, Roth TL, Talbot A, Nguyen DN, Chen YY, Blaeschke F, Vedova S, Mamedov MR, Chung JY, Li H, Wolf J, Martin TG, Ye L, Eyquem J, Esensten JH, Marson A.
• Abcc1 and Ggt5 support lymphocyte guidance through export and catabolism of S-geranylgeranyl-l-glutathione.
  Science immunology 2021 Gallman AE, Wolfreys FD, Nguyen DN, Sandy M, Xu Y, An J, Li Z, Marson A, Lu E, Cyster JG
• XYZeq: Spatially-resolved single-cell RNA-sequencing reveals expression heterogeneity in the tumor microenvironment
  Science Advances 2021 Lee Y*, Bogdanoff D*, Wang Y*, Hartoularos G, Woo JM, Mowery CT, Nisonoff HM, Lee DS, Sun Y, Lee J, Medhizadeh S, Cantlon J, Shifrut E, Nguyen DN, Song YS, Marson A, Chow ED, Ye CJ
• A diagnostic host response biosignature for COVID-19 from RNA profiling of nasal swabs and blood.
  Science advances 2021 Ng DL, Granados AC, Santos YA, Servellita V, Goldgof GM, Meydan C, Sotomayor-Gonzalez A, Levine AG, Balcerek J, Han LM, Akagi N, Truong K, Neumann NM, Nguyen DN, Bapat SP, Cheng J, Martin CS, Federman…
• Functional CRISPR dissection of gene networks controlling human regulatory T cell identity.
  Nature immunology 2020 Schumann K, Raju SS, Lauber M, Kolb S, Shifrut E, Cortez JT, Skartsis N, Nguyen VQ, Woo JM, Roth TL, Yu R, Nguyen MLT, Simeonov DR, Nguyen DN, Targ S, Gate RE, Tang Q, Bluestone JA, Spitzer MH, Ye CJ,…
• SARS-CoV-2 seroprevalence and neutralizing activity in donor and patient blood.
  Nature communications 2020 Ng DL, Goldgof GM, Shy BR, Levine AG, Balcerek J, Bapat SP, Prostko J, Rodgers M, Coller K, Pearce S, Franz S, Du L, Stone M, Pillai SK, Sotomayor-Gonzalez A, Servellita V, Martin CSS, Granados A, …
• Evaluation of SARS-CoV-2 serology assays reveals a range of test performance.
  Nature biotechnology 2020 Whitman JD, Hiatt J, Mowery CT, Shy BR, Yu R, Yamamoto TN, Rathore U, Goldgof GM, Whitty C, Woo JM, Gallman AE, Miller TE, Levine AG, Nguyen DN, Bapat SP, Balcerek J, Bylsma SA, Lyons AM, Li S, Wong …
• SARS-CoV-2 seroprevalence and neutralizing activity in donor and patient blood from the San Francisco Bay Area.
  medRxiv : the preprint server for health sciences 2020 Ng DL, Goldgof GM, Shy BR, Levine AG, Balcerek J, Bapat SP, Prostko J, Rodgers M, Coller K, Pearce S, Franz S, Du L, Stone M, Pillai SK, Sotomayor-Gonzalez A, Servellita V, Martin CSS, Granados A, …
• Test performance evaluation of SARS-CoV-2 serological assays.
  medRxiv : the preprint server for health sciences 2020 Whitman JD, Hiatt J, Mowery CT, Shy BR, Yu R, Yamamoto TN, Rathore U, Goldgof GM, Whitty C, Woo JM, Gallman AE, Miller TE, Levine AG, Nguyen DN, Bapat SP, Balcerek J, Bylsma SA, Lyons AM, Li S, Wong …
• Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies.
  Cell 2020 Roth TL, Li PJ, Blaeschke F, Nies JF, Apathy R, Mowery C, Yu R, Nguyen MLT, Lee Y, Truong A, Hiatt J, Wu D, Nguyen DN, Goodman D, Bluestone JA, Ye CJ, Roybal K, Shifrut E, Marson A
• Regulatory T cells use arginase 2 to enhance their metabolic fitness in tissues.
  JCI insight 2019 Lowe MM, Boothby I, Clancy S, Ahn RS, Liao W, Nguyen DN, Schumann K, Marson A, Mahuron KM, Kingsbury GA, Liu Z, Munoz Sandoval P, Rodriguez RS, Pauli ML, Taravati K, Arron ST, Neuhaus IM, Harris HW, …
• Reprogramming human T cell function and specificity with non-viral genome targeting.
  Nature 2018 Roth TL, Puig-Saus C, Yu R, Shifrut E, Carnevale J, Li PJ, Hiatt J, Saco J, Krystofinski P, Li H, Tobin V, Nguyen DN, Lee MR, Putnam AL, Ferris AL, Chen JW, Schickel JN, Pellerin L, Carmody D, Alkorta…
• Small-Molecule End-Groups of Linear Polymer Determine Cell-type Gene-Delivery Efficacy.
  Advanced materials (Deerfield Beach, Fla.) 2009 Sunshine J, Green JJ, Mahon KP, Yang F, Eltoukhy AA, Nguyen DN, Langer R, Anderson DG
• A novel high-throughput cell-based method for integrated quantification of type I interferons and in vitro screening of immunostimulatory RNA drug delivery.
  Biotechnology and bioengineering 2009 Nguyen DN, Kim P, Martínez-Sobrido L, Beitzel B, García-Sastre A, Langer R, Anderson DG
• A combinatorial library of lipid-like materials for delivery of RNAi therapeutics.
  Nature biotechnology 2008 Akinc A, Zumbuehl A, Goldberg M, Leshchiner ES, Busini V, Hossain N, Bacallado SA, Nguyen DN, Fuller J, Alvarez R, Borodovsky A, Borland T, Constien R, de Fougerolles A, Dorkin JR, Narayanannair …
• Enhancement of poly(orthoester) microspheres for DNA vaccine delivery by blending with poly(ethylenimine).
  Biomaterials 2008 Nguyen DN, Raghavan SS, Tashima LM, Lin EC, Fredette SJ, Langer RS, Wang C