Daniele Canzio, PhD
Assistant Professor
Neurology
School of Medicine

My laboratory is interested in understanding the molecular mechanism regulating the generation of cell-surface neural identities by Clustered Protocadherin genes. This process is of fundamental importance to the assembly of neural circuits in the mammalian brain.

Awards

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• Chan Zuckerberg Biohub Investigator Award, UCSF, 2022
• NIH Director New Innovator Award (DP2), UCSF, 2021
• Helen Hay Whitney Postdoctoral Research Fellowship, Columbia University, 2014-2017

Education & Training

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• Molecular Biology/Neuroscience Columbia University 12/2018
• PhD Chemical Biology University of California, San Francisco 12/2012
• BS Chemistry/Biochemistry University of California, Santa Barbara 03/2006

Websites

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• Profile in the Neuroscience Graduate Program (neurograd.ucsf.edu)
• Canzio Lab Website (canziolab.ucsf.edu)

Grants and Projects

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• How do neurons recognize self from non-self?, NIH, 2021-2024
• Coupling promoter choice and alternative RNA splicing in the mammalian Protocadherin gene cluster, NIH, 2017-2022
• Coupling promoter choice and alternative RNA splicing in the mammalian Protocadherin gene cluster, NIH, 2017-2019

Publications (15)

Top publication keywords:
Protein IsoformsRNA, Long NoncodingNucleosomesHeterochromatinRNA Polymerase IIEukaryotaSchizosaccharomycesSchizosaccharomyces pombe ProteinsChromosomal Proteins, Non-HistoneNeuronsCadherinsRNA, AntisenseDNA DemethylationCCCTC-Binding FactorChromatin Assembly and Disassembly

• WAPL functions as a rheostat of Protocadherin isoform diversity that controls neural wiring.
  Science (New York, N.Y.) 2023 Kiefer L, Chiosso A, Langen J, Buckley A, Gaudin S, Rajkumar SM, Servito GIF, Cha ES, Vijay A, Yeung A, Horta A, Mui MH, Canzio D
• STL-seq reveals pause-release and termination kinetics for promoter-proximal paused RNA polymerase II transcripts.
  Molecular cell 2021 Zimmer JT, Rosa-Mercado NA, Canzio D, Steitz JA, Simon MD
• The Loss of TBK1 Kinase Activity in Motor Neurons or in All Cell Types Differentially Impacts ALS Disease Progression in SOD1 Mice.
  Neuron 2020 Gerbino V, Kaunga E, Ye J, Canzio D, O'Keeffe S, Rudnick ND, Guarnieri P, Lutz CM, Maniatis T
• The generation of a protocadherin cell-surface recognition code for neural circuit assembly.
  Current opinion in neurobiology 2019 Canzio D, Maniatis T
• Clustered protocadherins methylation alterations in cancer.
  Clinical epigenetics 2019 Vega-Benedetti AF, Loi E, Moi L, Blois S, Fadda A, Antonelli M, Arcella A, Badiali M, Giangaspero F, Morra I, Columbano A, Restivo A, Zorcolo L, Gismondi V, Varesco L, Bellomo SE, Giordano S, Canale M…

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• Antisense lncRNA Transcription Mediates DNA Demethylation to Drive Stochastic Protocadherin α Promoter Choice.
  Cell 2019 Canzio D, Nwakeze CL, Horta A, Rajkumar SM, Coffey EL, Duffy EE, Duffié R, Monahan K, O'Keeffe S, Simon MD, Lomvardas S, Maniatis T
• Writing, Reading, and Translating the Clustered Protocadherin Cell Surface Recognition Code for Neural Circuit Assembly.
  Annual review of cell and developmental biology 2018 Mountoufaris G, Canzio D, Nwakeze CL, Chen WV, Maniatis T
• Solid phase chemistry to covalently and reversibly capture thiolated RNA.
  Nucleic acids research 2018 Duffy EE, Canzio D, Maniatis T, Simon MD
• CRISPR Inversion of CTCF Sites Alters Genome Topology and Enhancer/Promoter Function.
  Cell 2015 Guo Y, Xu Q, Canzio D, Shou J, Li J, Gorkin DU, Jung I, Wu H, Zhai Y, Tang Y, Lu Y, Wu Y, Jia Z, Li W, Zhang MQ, Ren B, Krainer AR, Maniatis T, Wu Q
• Mechanisms of functional promiscuity by HP1 proteins.
  Trends in cell biology 2014 Canzio D, Larson A, Narlikar GJ
• A conformational switch in HP1 releases auto-inhibition to drive heterochromatin assembly.
  Nature 2013 Canzio D, Liao M, Naber N, Pate E, Larson A, Wu S, Marina DB, Garcia JF, Madhani HD, Cooke R, Schuck P, Cheng Y, Narlikar GJ
• Structural basis of silencing: Sir3 BAH domain in complex with a nucleosome at 3.0 Å resolution.
  Science (New York, N.Y.) 2011 Armache KJ, Garlick JD, Canzio D, Narlikar GJ, Kingston RE
• Chromodomain-mediated oligomerization of HP1 suggests a nucleosome-bridging mechanism for heterochromatin assembly.
  Molecular cell 2011 Canzio D, Chang EY, Shankar S, Kuchenbecker KM, Simon MD, Madhani HD, Narlikar GJ, Al-Sady B
• Incorporation of positively charged ribonucleic guanidine linkages into oligodeoxyribonucleotides: Development of potent antisense agents.
  Bioorganic & medicinal chemistry letters 2008 Park M, Canzio D, Bruice TC
• DNA sequence recognition by Hoechst 33258 conjugates of hairpin pyrrole/imidazole polyamides.
  Bioorganic & medicinal chemistry letters 2006 Correa BJ, Canzio D, Kahane AL, Reddy PM, Bruice TC