Daniel Minor, JR, PhD
Professor
Cardiovascular Research Inst
School of Medicine

Education & Training

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• postdoc Ion channel structure and function University of California San Francisco 12/2000
• postdoc Ion channel structure MRC Laboratory of Molecular Biology 09/1996
• Ph.D. Biochemistry Massachusetts Institute of Technology 02/1996
• B.A. magna cum laude Biochemistry (Honors) Biophysics (Honors) University of Pennsylvania 05/1989

Websites

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• Minor lab website (cvri.ucsf.edu)
• Profile at cvri.ucsf.edu (cvri.ucsf.edu)

Grants and Projects

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• Structure and function of voltage-gated calcium channels, NIH, 2005-2024
• Structural Studies of Ion Channel Assembly and Signaling Complexes, NIH, 2005-2022
• Genetic and chemical biological studies of K2P structure, function, and modulation, NIH, 2011-2021
• Expanding the Chemical Biology of K2P Channels with Selective Cellular and In vivo Probes, NIH, 2018-2021
• Center for Structure of Membrane Proteins, NIH, 2010-2016
• Membrane Protein Expression Center, NIH, 2004-2015
• Bio-Organic Biomedical Mass Spectrometry Resource, NIH, 1982-2015
• Structural studies of CaV alpha2delta subunits and interaction with anti-nocicept, NIH, 2009-2013
• Discovery and characterization of ion channel modulators, NIH, 2004-2010
• Eukaryotic Ion Channel Expression for Structural Study, NIH, 2004-2006

Publications (79)

Top publication keywords:
CalciumKCNQ Potassium ChannelsCalcium Channels, L-TypeProtein Structure, TertiaryCalcium Channels, N-TypeVoltage-Gated Sodium ChannelsCrystallography, X-RayIon ChannelsIon Channel GatingCalmodulinSaxitoxinPotassium Channels, Tandem Pore DomainModels, MolecularCalcium ChannelsProtein Subunits

• Expression, purification, and characterization of anuran saxiphilins using thermofluor, fluorescence polarization, and isothermal titration calorimetry.
  STAR protocols 2023 Chen Z, Zakrzewska S, Hajare HS, Du Bois J, Minor DL
• Development of covalent chemogenetic K2P channel activators.
  bioRxiv : the preprint server for biology 2023 Deal PE, Lee H, Mondal A, Lolicato M, de Mendonca PRF, Black H, El-Hilali X, Bryant C, Isacoff EY, Renslo AR, Minor DL
• EMC chaperone-CaV structure reveals an ion channel assembly intermediate.
  Nature 2023 Chen Z, Mondal A, Abderemane-Ali F, Jang S, Niranjan S, Montaño JL, Zaro BW, Minor DL
• Structural basis for CaVα2δ:gabapentin binding.
  Nature structural & molecular biology 2023 Chen Z, Mondal A, Minor DL
• Selective posttranslational inhibition of CaVβ1-associated voltage-dependent calcium channels with a functionalized nanobody.
  Nature communications 2022 Morgenstern TJ, Nirwan N, Hernández-Ochoa EO, Bibollet H, Choudhury P, Laloudakis YD, Ben Johny M, Bannister RA, Schneider MF, Minor DL, Colecraft HM

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• Definition of a saxitoxin (STX) binding code enables discovery and characterization of the anuran saxiphilin family.
  Proceedings of the National Academy of Sciences of the United States of America 2022 Chen Z, Zakrzewska S, Hajare HS, Alvarez-Buylla A, Abderemane-Ali F, Bogan M, Ramirez D, O'Connell LA, Du Bois J, Minor DL
• Quaternary structure independent folding of voltage-gated ion channel pore domain subunits.
  Nature structural & molecular biology 2022 Arrigoni C, Lolicato M, Shaya D, Rohaim A, Findeisen F, Fong LK, Colleran CM, Dominik P, Kim SS, Schuermann JP, DeGrado WF, Grabe M, Kossiakoff AA, Minor DL
• Differential effects of modified batrachotoxins on voltage-gated sodium channel fast and slow inactivation.
  Cell chemical biology 2021 MacKenzie TMG, Abderemane-Ali F, Garrison CE, Minor DL, Bois JD
• Evidence that toxin resistance in poison birds and frogs is not rooted in sodium channel mutations and may rely on "toxin sponge" proteins.
  The Journal of general physiology 2021 Abderemane-Ali F, Rossen ND, Kobiela ME, Craig RA, Garrison CE, Chen Z, Colleran CM, O'Connell LA, Du Bois J, Dumbacher JP, Minor DL
• Inroads into Membrane Physiology through Transport Nanomachines.
  Journal of molecular biology 2021 Stockbridge RB, Gaudet R, Grabe M, Minor DL
• Ion Channels: Intersection of Structure, Function, and Pharmacology.
  Journal of molecular biology 2021 Moiseenkova-Bell V, Delemotte L, Minor DL
• Structural Insights into the Mechanisms and Pharmacology of K2P Potassium Channels.
  Journal of molecular biology 2021 Natale AM, Deal PE, Minor DL
• Production of K2P2.1 (TREK-1) for structural studies.
  Methods in enzymology 2021 Lee H, Lolicato M, Arrigoni C, Minor DL
• Preface.
  Methods in enzymology 2021 Minor DL, Colecraft HM
• Preface.
  Methods in enzymology 2021 Minor DL, Colecraft HM
• Preface.
  Methods in enzymology 2021 Minor DL, Colecraft HM
• The Polysite Pharmacology of TREK K2P Channels.
  Advances in experimental medicine and biology 2021 Pope L, Minor DL
• K2P channel C-type gating involves asymmetric selectivity filter order-disorder transitions.
  Science advances 2020 Lolicato M, Natale AM, Abderemane-Ali F, Crottès D, Capponi S, Duman R, Wagner A, Rosenberg JM, Grabe M, Minor DL
• Polynuclear Ruthenium Amines Inhibit K2P Channels via a "Finger in the Dam" Mechanism.
  Cell chemical biology 2020 Pope L, Lolicato M, Minor DL
• Structure of the saxiphilin:saxitoxin (STX) complex reveals a convergent molecular recognition strategy for paralytic toxins.
  Science advances 2019 Yen TJ, Lolicato M, Thomas-Tran R, Du Bois J, Minor DL
• SARAF Luminal Domain Structure Reveals a Novel Domain-Swapped β-Sandwich Fold Important for SOCE Modulation.
  Journal of molecular biology 2019 Kimberlin CR, Meshcheriakova A, Palty R, Raveh A, Karbat I, Reuveny E, Minor DL
• A Selectivity Filter Gate Controls Voltage-Gated Calcium Channel Calcium-Dependent Inactivation.
  Neuron 2019 Abderemane-Ali F, Findeisen F, Rossen ND, Minor DL
• Protein and Chemical Determinants of BL-1249 Action and Selectivity for K2P Channels.
  ACS chemical neuroscience 2018 Pope L, Arrigoni C, Lou H, Bryant C, Gallardo-Godoy A, Renslo AR, Minor DL
• A Calmodulin C-Lobe Ca2+-Dependent Switch Governs Kv7 Channel Function.
  Neuron 2018 Chang A, Abderemane-Ali F, Hura GL, Rossen ND, Gate RE, Minor DL
• Global versus local mechanisms of temperature sensing in ion channels.
  Pflugers Archiv : European journal of physiology 2018 Arrigoni C, Minor DL
• Structural Basis for Activity and Specificity of an Anticoagulant Anti-FXIa Monoclonal Antibody and a Reversal Agent.
  Structure (London, England : 1993) 2018 Ely LK, Lolicato M, David T, Lowe K, Kim YC, Samuel D, Bessette P, Garcia JL, Mikita T, Minor DL, Coughlin SR
• Cryo-EM structures of the TMEM16A calcium-activated chloride channel.
  Nature 2017 Dang S, Feng S, Tien J, Peters CJ, Bulkley D, Lolicato M, Zhao J, Zuberbühler K, Ye W, Qi L, Chen T, Craik CS, Jan YN, Minor DL, Cheng Y, Jan LY
• Channel surfing uncovers a dual-use transporter.
  The EMBO journal 2017 Minor DL
• K2P2.1 (TREK-1)-activator complexes reveal a cryptic selectivity filter binding site.
  Nature 2017 Lolicato M, Arrigoni C, Mori T, Sekioka Y, Bryant C, Clark KA, Minor DL
• Stapled Voltage-Gated Calcium Channel (CaV) α-Interaction Domain (AID) Peptides Act As Selective Protein-Protein Interaction Inhibitors of CaV Function.
  ACS chemical neuroscience 2017 Findeisen F, Campiglio M, Jo H, Abderemane-Ali F, Rumpf CH, Pope L, Rossen ND, Flucher BE, DeGrado WF, Minor DL
• Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation.
  Cell 2016 Arrigoni C, Rohaim A, Shaya D, Findeisen F, Stein RA, Nurva SR, Mishra S, Mchaourab HS, Minor DL
• Let It Go and Open Up, an Ensemble of Ion Channel Active States.
  Cell 2016 Minor DL
• Insights into the molecular foundations of electrical excitation.
  Journal of molecular biology 2015 Gaudet R, Roux B, Minor DL
• Transmembrane helix straightening and buckling underlies activation of mechanosensitive and thermosensitive K(2P) channels.
  Neuron 2014 Lolicato M, Riegelhaupt PM, Arrigoni C, Clark KA, Minor DL
• Bacterial voltage-gated sodium channels (BacNa(V)s) from the soil, sea, and salt lakes enlighten molecular mechanisms of electrical signaling and pharmacology in the brain and heart.
  Journal of molecular biology 2014 Payandeh J, Minor DL
• Tethered protein display identifies a novel Kir3.2 (GIRK2) regulator from protein scaffold libraries.
  ACS chemical neuroscience 2014 Bagriantsev SN, Chatelain FC, Clark KA, Alagem N, Reuveny E, Minor DL
• Conduits of life's spark: a perspective on ion channel research since the birth of neuron.
  Neuron 2013 Isacoff EY, Jan LY, Minor DL
• Structure of a prokaryotic sodium channel pore reveals essential gating elements and an outer ion binding site common to eukaryotic channels.
  Journal of molecular biology 2013 Shaya D, Findeisen F, Abderemane-Ali F, Arrigoni C, Wong S, Nurva SR, Loussouarn G, Minor DL
• Apo states of calmodulin and CaBP1 control CaV1 voltage-gated calcium channel function through direct competition for the IQ domain.
  Journal of molecular biology 2013 Findeisen F, Rumpf CH, Minor DL
• A high-throughput functional screen identifies small molecule regulators of temperature- and mechano-sensitive K2P channels.
  ACS chemical biology 2013 Bagriantsev SN, Ang KH, Gallardo-Godoy A, Clark KA, Arkin MR, Renslo AR, Minor DL
• Patch-clamp characterization of the MscS-like mechanosensitive channel from Silicibacter pomeroyi.
  Biophysical journal 2013 Petrov E, Palanivelu D, Constantine M, Rohde PR, Cox CD, Nomura T, Minor DL, Martinac B
• Identification of a dimerization domain in the TMEM16A calcium-activated chloride channel (CaCC).
  Proceedings of the National Academy of Sciences of the United States of America 2013 Tien J, Lee HY, Minor DL, Jan YN, Jan LY
• Using yeast to study potassium channel function and interactions with small molecules.
  Methods in molecular biology (Clifton, N.J.) 2013 Bagriantsev SN, Minor DL
• Structure of a Ca(2+)/CaM:Kv7.4 (KCNQ4) B-helix complex provides insight into M current modulation.
  Journal of molecular biology 2012 Xu Q, Chang A, Tolia A, Minor DL
• Metabolic and thermal stimuli control K(2P)2.1 (TREK-1) through modular sensory and gating domains.
  The EMBO journal 2012 Bagriantsev SN, Clark KA, Minor DL
• A glutamate switch controls voltage-sensitive phosphatase function.
  Nature structural & molecular biology 2012 Liu L, Kohout SC, Xu Q, Müller S, Kimberlin CR, Isacoff EY, Minor DL
• Chemical informatics and target identification in a zebrafish phenotypic screen.
  Nature chemical biology 2011 Laggner C, Kokel D, Setola V, Tolia A, Lin H, Irwin JJ, Keiser MJ, Cheung CY, Minor DL, Roth BL, Peterson RT, Shoichet BK
• Multiple modalities converge on a common gate to control K2P channel function.
  The EMBO journal 2011 Bagriantsev SN, Peyronnet R, Clark KA, Honoré E, Minor DL
• Voltage-gated sodium channel (NaV) protein dissection creates a set of functional pore-only proteins.
  Proceedings of the National Academy of Sciences of the United States of America 2011 Shaya D, Kreir M, Robbins RA, Wong S, Hammon J, Brüggemann A, Minor DL
• Calmodulin overexpression does not alter Cav1.2 function or oligomerization state.
  Channels (Austin, Tex.) 2011 Findeisen F, Tolia A, Arant R, Kim EY, Isacoff E, Minor DL
• Structural basis for the differential effects of CaBP1 and calmodulin on Ca(V)1.2 calcium-dependent inactivation.
  Structure (London, England : 1993) 2010 Findeisen F, Minor DL
• Progress in the structural understanding of voltage-gated calcium channel (CaV) function and modulation.
  Channels (Austin, Tex.) 2010 Minor DL, Findeisen F
• Multiple C-terminal tail Ca(2+)/CaMs regulate Ca(V)1.2 function but do not mediate channel dimerization.
  The EMBO journal 2010 Kim EY, Rumpf CH, Van Petegem F, Arant RJ, Findeisen F, Cooley ES, Isacoff EY, Minor DL
• Small molecule ion channel match making: a natural fit for new ASIC ligands.
  Neuron 2010 Bagriantsev SN, Minor DL
• Electrochemical coupling in the voltage-dependent phosphatase Ci-VSP.
  Nature chemical biology 2010 Kohout SC, Bell SC, Liu L, Xu Q, Minor DL, Isacoff EY
• Selection of inhibitor-resistant viral potassium channels identifies a selectivity filter site that affects barium and amantadine block.
  PloS one 2009 Chatelain FC, Gazzarrini S, Fujiwara Y, Arrigoni C, Domigan C, Ferrara G, Pantoja C, Thiel G, Moroni A, Minor DL
• Crystal structure of a trimeric form of the K(V)7.1 (KCNQ1) A-domain tail coiled-coil reveals structural plasticity and context dependent changes in a putative coiled-coil trimerization motif.
  Protein science : a publication of the Protein Society 2009 Xu Q, Minor DL
• Searching for interesting channels: pairing selection and molecular evolution methods to study ion channel structure and function.
  Molecular bioSystems 2009 Minor DL
• Disruption of the IS6-AID linker affects voltage-gated calcium channel inactivation and facilitation.
  The Journal of general physiology 2009 Findeisen F, Minor DL
• 2007 annual progress report synopsis of the Center for Structures of Membrane Proteins.
  Journal of structural and functional genomics 2009 Stroud RM, Choe S, Holton J, Kaback HR, Kwiatkowski W, Minor DL, Riek R, Sali A, Stahlberg H, Harries W
• A green fluorescent protein screen for identification of well-expressed membrane proteins from a cohort of extremophilic organisms.
  Protein science : a publication of the Protein Society 2009 Hammon J, Palanivelu DV, Chen J, Patel C, Minor DL
• Structures of CaV2 Ca2+/CaM-IQ domain complexes reveal binding modes that underlie calcium-dependent inactivation and facilitation.
  Structure (London, England : 1993) 2008 Kim EY, Rumpf CH, Fujiwara Y, Cooley ES, Van Petegem F, Minor DL
• X-ray crystal structure of a TRPM assembly domain reveals an antiparallel four-stranded coiled-coil.
  Journal of molecular biology 2008 Fujiwara Y, Minor DL
• Transmembrane domain length of viral K+ channels is a signal for mitochondria targeting.
  Proceedings of the National Academy of Sciences of the United States of America 2008 Balss J, Papatheodorou P, Mehmel M, Baumeister D, Hertel B, Delaroque N, Chatelain FC, Minor DL, Van Etten JL, Rassow J, Moroni A, Thiel G
• Alanine-scanning mutagenesis defines a conserved energetic hotspot in the CaValpha1 AID-CaVbeta interaction site that is critical for channel modulation.
  Structure (London, England : 1993) 2008 Van Petegem F, Duderstadt KE, Clark KA, Wang M, Minor DL
• Puzzle plugged by protein pore plasticity.
  Molecular cell 2007 Minor DL
• The neurobiologist's guide to structural biology: a primer on why macromolecular structure matters and how to evaluate structural data.
  Neuron 2007 Minor DL
• Structural insight into KCNQ (Kv7) channel assembly and channelopathy.
  Neuron 2007 Howard RJ, Clark KA, Holton JM, Minor DL
• Three-dimensional structure of the KChIP1-Kv4.3 T1 complex reveals a cross-shaped octamer.
  Nature structural & molecular biology 2006 Pioletti M, Findeisen F, Hura GL, Minor DL
• Coiled coils direct assembly of a cold-activated TRP channel.
  Neuron 2006 Tsuruda PR, Julius D, Minor DL
• Wanting contact: how to pick up a channel.
  Nature chemical biology 2006 Minor DL
• A multimeric membrane protein reveals 14-3-3 isoform specificity in forward transport in yeast.
  Traffic (Copenhagen, Denmark) 2006 Michelsen K, Mrowiec T, Duderstadt KE, Frey S, Minor DL, Mayer MP, Schwappach B
• A sensitive channel family replete with sense and motion.
  Nature structural & molecular biology 2006 Minor DL
• Insights into voltage-gated calcium channel regulation from the structure of the CaV1.2 IQ domain-Ca2+/calmodulin complex.
  Nature structural & molecular biology 2005 Van Petegem F, Chatelain FC, Minor DL
• The pore helix dipole has a minor role in inward rectifier channel function.
  Neuron 2005 Chatelain FC, Alagem N, Xu Q, Pancaroglu R, Reuveny E, Minor DL
• Bend to open?
  Structure (London, England : 1993) 2005 Minor DL
• Structure of a complex between a voltage-gated calcium channel beta-subunit and an alpha-subunit domain.
  Nature 2004 Van Petegem F, Clark KA, Chatelain FC, Minor DL
• The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1.
  Molecular cell 2003 Walden H, Podgorski MS, Huang DT, Miller DW, Howard RJ, Minor DL, Holton JM, Schulman BA
• Consensus-derived structural determinants of the ankyrin repeat motif.
  Proceedings of the National Academy of Sciences of the United States of America 2002 Mosavi LK, Minor DL, Peng ZY