Celine Taglang, PhD
Associate Specialist
Radiology
School of Medicine
Post-doctoral researcher at UCSF, with a background in chemistry, working on 13C labeling and hyperpolarization of biocompatible agents for MRI with applications from enzymatic activity to animal models, my current research, in Chaumeil Lab, focuses on the evaluation of strategies to attenuate primary CNS lymphoma growth by testing promising agents with or without established therapies.
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I completed my PhD degree in 2015 at the Paris Saclay University, after three internships in organic and medicinal chemistry in two private companies (GSK, Sanofi) and one public institution (CEA). In the course of my trainings, I worked on the synthesis of anti-cancer and anti-diabetic therapeutic molecules and discovered my passion for Research in life sciences.
During my PhD, my research focused on the enantiospecific C(sp3)-H activation followed by the deuterium incorporation onto stereogenic centers of small molecules. Using ruthenium nanoparticles, I applied this powerful and general method on 25 important chemical and biological compounds such as amines, aminoacids and small peptides. The mechanism of this fully stereoretentive process was investigated by a set of chemical experiments and DFT calculations which led to infer the reaction implied an unprecedented mechanism involving multiple ruthenium atoms and a σ-complex-assisted metathesis.
After these diverse experiences, I decided to join Dr. Wilson's lab at UCSF: in hyperpolarized 13C MRI, one of the fundamental limitations is the effective lifetime of the signal, called T1. I developed a robust late stage deuteration methodology, which is broadly applicable to amino, and α-hydroxyl acids, like alanine and lactate. Incorporation of deuterium in these substrates led to a significant T1 prolongation, ranging from 16-29% at a position adjacent to the 13C nucleus. Moreover, when applied to directly attached 13C nuclei, this led to a greater than 4 fold increase in T1. Most importantly, when applied to in vivo imaging, [1-13C,2-2H]alanine demonstrated a greater than doubling of effective signal to noise ratio. I was also working with Dr. Flavell's lab on molecular strategies to image the acidic tumoral microenvironment. For example, [2-13C,D10]diethylmalonic acid is a strong potential candidate for high spatial resolution in vivo pH mapping. Instead of using a ratiometric method, it uses differences in chemical shifts and allows for the detection of multiple pH compartments within the same voxel in mice kidneys imaging experiments.
Awards
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- 2016 Prostate Cancer Research Program - Early Investigator Research Award, US Department of Defense Office of the Congressionally Directed Medical Research Programs, 2017-2019
- IRTELIS-CEA PhD Program-Life Sciences Division, French Atomic Energy Commission, 2012-2015
Education & Training
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- Postdoctoral research 13C labeling and hyperpolarization of biocompatible agents for MRI University of California San Francisco, Department of Radiology and Biomedical Imaging 2019
- Ph.D in Organic Chemistry Enantiospecific C(sp3)-H activation catalyzed by ruthenium nanoparticles and isotopic labeling French Atomic Energy Commission - Paris Saclay University 2015
- Synthesis of chitosan NHC-N heterogeneous catalysts and study of their reactivity French Atomic Energy Commission - Paris Saclay University 2012
- Synthesis of anti-diabetic therapeutic molecules GlaxoSmithKline, Research Center, Les Ulis 2011
- Synthesis of kinase inhibitors Sanofi, Medicinal Chemistry – Oncology 2010
- Postdoctoral research Evaluation of strategies to attenuate primary CNS lymphoma growth by testing promising agents University of California San Francisco, Department of Physical Therapy
Interests
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- C-H activation
- HPLC
- Nuclear Magnetic Resonance
- Lymphoma
- Mass spectrometry
- Hyperpolarized 13C MRI
- Isotopic labeling
- Prostate cancer
- Nanoparticles
- pH Imaging
- Chemistry
Publications (13)
Top publication keywords:
4-Aminobenzoic AcidTelomeraseBacteriaCarbon RadioisotopesOligodendrogliomaDeuteriumNeuroimagingCarbon IsotopesGlioblastomaAza CompoundsRadioactive TracersGA-Binding Protein Transcription FactorMagnetic Resonance SpectroscopyDicarboxylic AcidsRuthenium
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Imaging telomerase reverse transcriptase expression in oligodendrogliomas using hyperpolarized δ-[1-13C]-gluconolactone.
Neuro-oncology advances 2023 Batsios G, Taglang C, Gillespie AM, Viswanath P -
Hyperpolarized δ-[1- 13C]gluconolactone imaging visualizes response to TERT or GABPB1 targeting therapy for glioblastoma.
Scientific reports 2023 Minami N, Hong D, Taglang C, Batsios G, Gillespie AM, Viswanath P, Stevers N, Barger CJ, Costello JF, Ronen SM -
Deuterium Metabolic Imaging Reports on TERT Expression and Early Response to Therapy in Cancer.
Clinical cancer research : an official journal of the American Association for Cancer Research 2022 Batsios G, Taglang C, Tran M, Stevers N, Barger C, Gillespie AM, Ronen SM, Costello JF, Viswanath P -
Metabolic imaging detects elevated glucose flux through the pentose phosphate pathway associated with TERT expression in low-grade gliomas.
Neuro-oncology 2021 Viswanath P, Batsios G, Ayyappan V, Taglang C, Gillespie AM, Larson PEZ, Luchman HA, Costello JF, Pieper RO, Ronen SM -
Imaging 6-Phosphogluconolactonase Activity in Brain Tumors In Vivo Using Hyperpolarized δ-[1-13C]gluconolactone.
Frontiers in oncology 2021 Batsios G, Taglang C, Cao P, Gillespie AM, Najac C, Subramani E, Wilson DM, Flavell RR, Larson PEZ, Ronen SM, Viswanath P
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Imaging Brain Metabolism Using Hyperpolarized 13C Magnetic Resonance Spectroscopy.
Trends in neurosciences 2020 Le Page LM, Guglielmetti C, Taglang C, Chaumeil MM -
Amino Acid-Derived Sensors for Specific Zn2+ Detection Using Hyperpolarized 13 C Magnetic Resonance Spectroscopy.
Chemistry (Weinheim an der Bergstrasse, Germany) 2019 Wang S, Korenchan DE, Perez PM, Taglang C, Hayes TR, Sriram R, Bok R, Hong AS, Wu Y, Li H, Wang Z, Kurhanewicz J, Wilson DM, Flavell RR -
Late-stage deuteration of 13C-enriched substrates for T1 prolongation in hyperpolarized 13C MRI.
Chemical communications (Cambridge, England) 2018 Taglang C, Korenchan DE, von Morze C, Yu J, Najac C, Wang S, Blecha JE, Subramaniam S, Bok R, VanBrocklin HF, Vigneron DB, Ronen SM, Sriram R, Kurhanewicz J, Wilson DM, Flavell RR -
[11C]Para-Aminobenzoic Acid: A Positron Emission Tomography Tracer Targeting Bacteria-Specific Metabolism.
ACS infectious diseases 2018 Mutch CA, Ordonez AA, Qin H, Parker M, Bambarger LE, Villanueva-Meyer JE, Blecha J, Carroll V, Taglang C, Flavell R, Sriram R, VanBrocklin H, Rosenberg O, Ohliger MA, Jain SK, Neumann KD, Wilson DM -
Ruthenium-catalyzed hydrogen isotope exchange of C(sp3)-H bonds directed by a sulfur atom.
Chemical communications (Cambridge, England) 2018 Gao L, Perato S, Garcia-Argote S, Taglang C, Martínez-Prieto LM, Chollet C, Buisson DA, Dauvois V, Lesot P, Chaudret B, Rousseau B, Feuillastre S, Pieters G -
Dicarboxylic acids as pH sensors for hyperpolarized 13C magnetic resonance spectroscopic imaging.
The Analyst 2017 Korenchan DE, Taglang C, von Morze C, Blecha JE, Gordon JW, Sriram R, Larson PEZ, Vigneron DB, VanBrocklin HF, Kurhanewicz J, Wilson DM, Flavell RR -
Enantiospecific C-H Activation Using Ruthenium Nanocatalysts.
Angewandte Chemie (International ed. in English) 2015 Taglang C, Martínez-Prieto LM, del Rosal I, Maron L, Poteau R, Philippot K, Chaudret B, Perato S, Sam Lone A, Puente C, Dugave C, Rousseau B, Pieters G -
Regioselective and stereospecific deuteration of bioactive aza compounds by the use of ruthenium nanoparticles.
Angewandte Chemie (International ed. in English) 2013 Pieters G, Taglang C, Bonnefille E, Gutmann T, Puente C, Berthet JC, Dugave C, Chaudret B, Rousseau B