Bruce Conklin, MD
Professor
Medicine
School of Medicine
bconklin@gladstone.ucsf.edu 415-734-2000
Therapeutic Approaches to Genetic Disease
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Genome Engineering The late Richard Feynman once said, “What I cannot create, I do not understand.” Human genetics began as observational science, but newly developed genome-engineering tools now allow us to directly test the cellular consequences of discrete genetic changes. We have developed efficient methods to edit one residue at a time in living human induced pluripotent stem (iPS) cells, resulting in “isogenic” lines of iPS cells. These isogenic lines form models that are now yielding phenotypes that are helping to explain the molecular basis of several human diseases. We are constructing collections of these isogenic lines that carry a range of disease mutations, from the most severe (rare) to the moderate (common) forms of cardiomyopathy.
Precise Genome Editing for Human Therapy The rapid development of genome engineering, such as with the CRISPR system, now allows us to contemplate using these tools for human therapy in selective tissues. Using genome editing to “fix” a disease gene has great promise, because it could permanently correct the disease. However, tremendous challenges remain to ensure that only a specific part of the genome is edited, without causing “off-target” DNA damage that could lead to cancer. We focused on developing therapeutic editing for diseases of the heart and retina, because each tissue has unique challenges and opportunities. The heart has many newly described gene mutations that result in lethal heart failure (cardiomyopathy). Genome engineering can be used to cure cardiomyopathy mutations and validate targets for drug therapy; however, current engineering methods will need to be greatly enhanced before it can be used in vivo in the heart. In contrast, the retina has the advantage of a limited number of cells that can be directly targeted for therapy. These properties could potentially cure blindness caused by many gene mutations that currently have no therapy. We are developing proof-of-concept therapeutic editing methods in the retina that can be expanded to other tissues, such as the heart.
Human Cardiac Disease Models We use iPS cells to model the genetic causes of human cardiomyopathy. Surprisingly, little is known about many genes associated with heart failure, from cardiomyopathy to abnormal heart rhythm, that results in “sudden death.” The heart provides an ideal system to determine the molecular basis of gene associations in human genetics. Until recently, modeling human gene variants in human cardiac tissue has been impossible. Human iPS cells now allow us to produce cardiovascular tissues that are identical, except for a single gene that has been altered by genome engineering. Our efforts have already been used to uncover multiple disease phenotypes and targets for drug therapy.
CRISPR-Based Screens in Human Cardiac Disease Models Recently, we developed CRISPR-inhibition (CRISPRi) cell lines for highthroughput gene inactivation of thousands of different genes. CRISPRi screens can be used to identify the molecular basis of development, so that we can construct more mature human tissues and improved disease models. These studies will also allow us to identify drug targets that could be used for treating cardiomyopathy and other major diseases. We are currently focused on finding new pathways to enhance cardiac regeneration. In the future, other versions of the CRISPR system could be used to activate genes and control the epigenetic state of the genome.
Future Directions We are developing new genome engineering methods in human iPS cells to identify therapeutic targets in cardiac disease. We are using these same tools to develop strategies for therapeutic genome editing. The combination of human iPS cells and genome editing provide unprecedented opportunities to explore new areas of biology and discover new therapies for disease.
Selected Recent Publications 1. Mandegar MA, Huebsch N, Frolov E, SHin E, Weissman JS, Qi LS, So P-L, Conklin BR. (2016) CRISPR interference efficiently induces gene knockdown and models disease in iPSCs. Cell Stem Cell. 18 1-13, April 7, 2016 2. Huebsch N et al. (2015) Automated video-based analysis of contractility and calcium flux in human-induced pluripotent stem cell-derived cardiomyocytes cultured over different spatial scales. Tissue Eng. Part C Methods 21:467. 3. Ma Z et al. (2015) Self-organizing human cardiac microchambers mediated by geometric confinement. Nat. Commun. 6:7413. 4. Miyaoka Y et al. (2014) Isolation of single-base genome-edited human iPS cells without antibiotic selection. Nat. Methods 11:291. 5. Spencer CI et al. (2014) Calcium transients closely reflect prolonged action potentials in iPSC models of inherited cardiac arrhythmia. Stem Cell Reports 3:269.
Awards
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- CWRU School of Medicine Distinguished Alumni Award, 2016
- Fellow, California Academy of Sciences, 2011
- Scientific American 50 Award, 2008
- American Society for Clinical Investigation, 2003
- Medical Resident Research Award-NIDDK, 1990
Education & Training
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- DEI Champion University of California San Francisco 2021
- Molecular Pharmacology University of California San Francisco 01/1995
- Internal Medicine Johns Hopkins Hospital 06/1990
- M.D. Medicine Case Western Reserve School of Medicine 06/1988
- A.B. Public Health University of California, Berkeley, CA 06/1982
Interests
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- Genome Engineering
- Cardiomyopathy
- Genetics
- iPS cells
Websites
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- UCSF Biomedical sciences (bms.ucsf.edu)
- Conklin Lab (labs.gladstone.org)
- UCSF Division of Cardiology (ucsfhealthcardiology.ucsf.edu)
- Profile at endocrine.medicine.ucsf.edu (endocrine.medicine.ucsf.edu)
Grants and Projects
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- C9orf72 frontotemporal dementia (FTD) and amyotrophic lateral sclerosis(ALS): using patient cells and CRISPR to reveal therapeutic approaches, NIH, 2021-2024
- Therapeutic genome editing to treat Best disease, NIH, 2017-2022
- Protein quality control, cardiomyopathy, cardiotoxicity and human isogenic iPSCs, NIH, 2017-2021
- Identifying Therapeutic Targets for RNA Splicing-Related Cardiomyopathy, NIH, 2015-2019
- Transcriptional Networks During Cardiac Differentiation, NIH, 2007-2018
- Induced Pluripotent Stem Cells in the Understanding and Treatment of Heart Diseas, NIH, 2009-2016
- Structures of Protein Complexes Regulating Transcription in Enbryonic Stem Cells, NIH, 2010-2016
- The Epigenetic Landscape of Heart Development, NIH, 2009-2015
- Disease Specific Cardiac Tissue Models, NIH, 2011-2015
- Tissue Engineering with a Modular RASSL Toolbox, NIH, 1998-2013
- GenMAPP-CS, a dynamic resource pathway analysis, NIH, 2004-2012
- Induced pluripotent stem cells in the understanding and treatment of heart diseas, NIH, 2008-2009
- GenMAPP: A tool for pathway analysis of genomic data, NIH, 2004-2007
- Collaborative R01--Roles of G Proteins in Cardiomyopathy, NIH, 1998-2003
- Mutant G Proteins Reveal Signaling Pathways, NIH, 1991-1995
Publications (180)
Top publication keywords:
GTP-Binding Protein alpha Subunits, Gi-GoCRISPR-Cas SystemsPolymerase Chain ReactionGene EditingDNA End-Joining RepairNucleotidesRecombinational DNA RepairPluripotent Stem CellsReceptors, G-Protein-CoupledReceptors, Opioid, kappaGTP-Binding Protein alpha Subunits, GsClustered Regularly Interspaced Short Palindromic RepeatsInduced Pluripotent Stem CellsMyocytes, CardiacEmbryonic Stem Cells
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Allele-Specific Gene Editing Rescues Pathology in a Human Model of Charcot-Marie-Tooth Disease Type 2E.
Frontiers in cell and developmental biology 2021 Feliciano CM, Wu K, Watry HL, Marley CBE, Ramadoss GN, Ghanim HY, Liu AZ, Zholudeva LV, McDevitt TC, Saporta MA, Conklin BR, Judge LM -
SARS-CoV-2 infection of human iPSC-derived cardiac cells reflects cytopathic features in hearts of patients with COVID-19.
Science translational medicine 2021 Perez-Bermejo JA, Kang S, Rockwood SJ, Simoneau CR, Joy DA, Silva AC, Ramadoss GN, Flanigan WR, Fozouni P, Li H, Chen PY, Nakamura K, Whitman JD, Hanson PJ, McManus BM, Ott M, Conklin BR, McDevitt TC -
Rapid, precise quantification of large DNA excisions and inversions by ddPCR.
Scientific reports 2020 Watry HL, Feliciano CM, Gjoni K, Takahashi G, Miyaoka Y, Conklin BR, Judge LM -
AlleleAnalyzer: a tool for personalized and allele-specific sgRNA design.
Genome biology 2019 Keough KC, Lyalina S, Olvera MP, Whalen S, Conklin BR, Pollard KS -
Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq.
Science (New York, N.Y.) 2019 Wienert B, Wyman SK, Richardson CD, Yeh CD, Akcakaya P, Porritt MJ, Morlock M, Vu JT, Kazane KR, Watry HL, Judge LM, Conklin BR, Maresca M, Corn JE -
A BAG3 chaperone complex maintains cardiomyocyte function during proteotoxic stress.
JCI insight 2017 Judge LM, Perez-Bermejo JA, Truong A, Ribeiro AJ, Yoo JC, Jensen CL, Mandegar MA, Huebsch N, Kaake RM, So PL, Srivastava D, Pruitt BL, Krogan NJ, Conklin BR -
CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.
Cell stem cell 2016 Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP,… -
Isolation of single-base genome-edited human iPS cells without antibiotic selection.
Nature methods 2014 Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga PP, So PL, Conklin BR -
Sculpting genomes with a hammer and chisel.
Nature methods 2013 Conklin BR
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Dual a-globin-truncated erythropoietin receptor knockin restores hemoglobin production in a-thalassemia-derived erythroid cells.
Cell reports 2025 Chu SN, Soupene E, Sharma D, Sinha R, McCreary T, Hernandez B, Shen H, Wienert B, Bowman C, Yin H, Lesch BJ, Jia K, Romero KA, Kostamo Z, Zhang Y, Tran T, Cordero M, Homma S, Hampton JP, Gardner JM, … -
Haplotype editing with CRISPR/Cas9 as a therapeutic approach for dominant-negative missense mutations in NEFL.
bioRxiv : the preprint server for biology 2024 Dua PH, Simon BMJ, Marley CBE, Feliciano CM, Watry HL, Steury D, Abraham A, Gilbertson EN, Ramey GD, Capra JA, Conklin BR, Judge LM -
Generation and characterization of an isogenic control line by correcting the BAG3 P209L mutation of a human induced pluripotent stem cell (hiPSC) line from a patient with myofibrillar myopathy-6.
Stem cell research 2024 Filippi K, Riße I, Judge LM, Conklin BR, Fleischmann BK, Hesse M -
Reversal of C9orf72 mutation-induced transcriptional dysregulation and pathology in cultured human neurons by allele-specific excision.
Alzheimer's & Dementia 2024 Aradhana Sachdev, Kamaljot Gil, Maria Sckaff, Alisha M Birk, Olubankole Aladesuyi Arogundade, Katherine A Brown, Runvir S Chouhan, Patrick Issagholian Lewin, Esha Patel, Kathleen Keough, Bruce Conklin… -
Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteins.
bioRxiv : the preprint server for biology 2024 Xu D, Besselink S, Ramadoss GN, Dierks PH, Lubin JP, Pattali RK, Brim JI, Christenson AE, Colias PJ, Ornelas IJ, Nguyen CD, Chasins SE, Conklin BR, Nuñez JK -
Neuronal DNA repair reveals strategies to influence CRISPR editing outcomes.
bioRxiv : the preprint server for biology 2024 Ramadoss GN, Namaganda SJ, Hamilton JR, Sharma R, Chow KG, Macklin BL, Sun M, Liu JC, Fellmann C, Watry HL, Jin J, Perez BS, Sandoval Espinoza CR, Matia MP, Lu SH, Judge LM, Nussenzweig A, Adamson B, … -
Permittivity tensor imaging: modular label-free imaging of 3D dry mass and 3D orientation at high resolution.
Nature methods 2024 Yeh LH, Ivanov IE, Chandler T, Byrum JR, Chhun BB, Guo SM, Foltz C, Hashemi E, Perez-Bermejo JA, Wang H, Yu Y, Kazansky PG, Conklin BR, Han MH, Mehta SB -
Advances and challenges in modeling inherited peripheral neuropathies using iPSCs.
Experimental & molecular medicine 2024 Van Lent J, Prior R, Pérez Siles G, Cutrupi AN, Kennerson ML, Vangansewinkel T, Wolfs E, Mukherjee-Clavin B, Nevin Z, Judge L, Conklin B, Tyynismaa H, Clark AJ, Bennett DL, Van Den Bosch L, Saporta M,… -
Dual α-globin and truncated EPO receptor knockin restores hemoglobin production in α-thalassemia-derived red blood cells.
bioRxiv : the preprint server for biology 2024 Chu SN, Soupene E, Wienert B, Yin H, Sharma D, McCreary T, Jia K, Homma S, Hampton JP, Gardner JM, Conklin BR, MacKenzie TC, Porteus MH, Cromer MK -
Reversal of C9orf72 mutation-induced transcriptional dysregulation and pathology in cultured human neurons by allele-specific excision.
Proceedings of the National Academy of Sciences of the United States of America 2024 Sachdev A, Gill K, Sckaff M, Birk AM, Aladesuyi Arogundade O, Brown KA, Chouhan RS, Issagholian-Lewin PO, Patel E, Watry HL, Bernardi MT, Keough KC, Tsai YC, Smith AST, Conklin BR, Clelland CD -
Give heart cells a beat: An interactive museum exhibit that synchronizes stem cell-derived cardiomyocytes to visitors' heartbeat.
Stem cell reports 2024 Perez-Bermejo JA, Reisman SJ, Ma J, Carrison-Stone D, Cerrito C, Ribeiro AJS, Conklin BR, Yu K -
Validated assays for the quantification of C9orf72 human pathology.
Scientific reports 2024 Salomonsson SE, Maltos AM, Gill K, Aladesuyi Arogundade O, Brown KA, Sachdev A, Sckaff M, Lam KJK, Fisher IJ, Chouhan RS, Van Laar VS, Marley CB, McLaughlin I, Bankiewicz KS, Tsai YC, Conklin BR, … -
Dual a-Globin and Truncated EPO Receptor Knockin Restores Hemoglobin Production in a-Thalassemia-Derived Hematopoietic Stem and Progenitor Cells.
Blood 2023 Simon N Chu, Eric Soupene, Beeke Wienert, Han Yin, Devesh Sharma, Kun Jia, Shota Homma, Jessica P Hampton, Bruce Conklin, Tippi C. MacKenzie, Matthew Porteus, Kyle Cromer -
Functional analysis of a common BAG3 allele associated with protection from heart failure.
Nature cardiovascular research 2023 Perez-Bermejo JA, Judge LM, Jensen CL, Wu K, Watry HL, Truong A, Ho JJ, Carter M, Runyon WV, Kaake RM, Pulido EH, Mandegar MA, Swaney DL, So PL, Krogan NJ, Conklin BR -
Evaluation of CRISPR/Cas9 exon-skipping vector for choroideremia using human induced pluripotent stem cell-derived RPE.
The journal of gene medicine 2022 Iwagawa T, Masumoto H, Tabuchi H, Tani K, Conklin BR, Watanabe S -
A reference human induced pluripotent stem cell line for large-scale collaborative studies.
Cell stem cell 2022 Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, Oguro H, Kanaujiya J, Zou J, Sebesta D, Pratt G, Cross E, Blockwick J, Buxton P, Kinner-Bibeau L, Medura C, Tompkins C, Hughes S, … -
Transcription Factor GATA4 Regulates Cell Type-Specific Splicing Through Direct Interaction With RNA in Human Induced Pluripotent Stem Cell-Derived Cardiac Progenitors.
Circulation 2022 Zhu L, Choudhary K, Gonzalez-Teran B, Ang YS, Thomas R, Stone NR, Liu L, Zhou P, Zhu C, Ruan H, Huang Y, Jin S, Pelonero A, Koback F, Padmanabhan A, Sadagopan N, Hsu A, Costa MW, Gifford CA, van … -
Transcription factor protein interactomes reveal genetic determinants in heart disease.
Cell 2022 Gonzalez-Teran B, Pittman M, Felix F, Thomas R, Richmond-Buccola D, Hüttenhain R, Choudhary K, Moroni E, Costa MW, Huang Y, Padmanabhan A, Alexanian M, Lee CY, Maven BEJ, Samse-Knapp K, Morton SU, … -
BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2.
Nature cell biology 2022 Samelson AJ, Tran QD, Robinot R, Carrau L, Rezelj VV, Kain AM, Chen M, Ramadoss GN, Guo X, Lim SA, Lui I, Nuñez JK, Rockwood SJ, Wang J, Liu N, Carlson-Stevermer J, Oki J, Maures T, Holden K, Weissman… -
Abstract 11332: Integration of Protein Interactome Networks with Congenital Heart Disease Variants Reveals Candidate Disease Genes.
Circulation 2021 Barbara Gonzalez Teran, Maureen Pittman, Reuben Thomas, Franco Felix, Desmond Richmond-Buccola, Krishna Choudhary, Elisabetta Moroni, Colombo Giorgio, Arun Padmanabhan, Mauro Costa, Yu Huang, Michael … -
Gain-of-function cardiomyopathic mutations in RBM20 rewire splicing regulation and re-distribute ribonucleoprotein granules within processing bodies.
Nature communications 2021 Fenix AM, Miyaoka Y, Bertero A, Blue SM, Spindler MJ, Tan KKB, Perez-Bermejo JA, Chan AH, Mayerl SJ, Nguyen TD, Russell CR, Lizarraga PP, Truong A, So PL, Kulkarni A, Chetal K, Sathe S, Sniadecki NJ, … -
BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2.
bioRxiv : the preprint server for biology 2021 Samelson AJ, Tran QD, Robinot R, Carrau L, Rezelj VV, Mac Kain A, Chen M, Ramadoss GN, Guo X, Lim SA, Lui I, Nunez J, Rockwood SJ, Wang J, Liu N, Carlson-Stevermer J, Oki J, Maures T, Holden K, … -
Bioengineered optogenetic model of human neuromuscular junction.
Biomaterials 2021 Vila OF, Chavez M, Ma SP, Yeager K, Zholudeva LV, Colón-Mercado JM, Qu Y, Nash TR, Lai C, Feliciano CM, Carter M, Kamm RD, Judge LM, Conklin BR, Ward ME, McDevitt TC, Vunjak-Novakovic G -
Transcription factor overexpression drives reliable differentiation of retinal pigment epithelium from human induced pluripotent stem cells.
Stem cell research 2021 Dewell TE, Gjoni K, Liu AZ, Libby ARG, Moore AT, So PL, Conklin BR -
Investigating Zeta Globin Gene Expression to Develop a Potential Therapy for Alpha Thalassemia Major.
Blood 2020 Georgia L. Gregory, Beeke Wienert, Marisa Schwab, Billie Rachael Lianoglou, Roger P. Hollis, Donald B. Kohn, Bruce R. Conklin, Tippi C. MacKenzie -
SARS-CoV-2 infection of human iPSC-derived cardiac cells predicts novel cytopathic features in hearts of COVID-19 patients.
bioRxiv : the preprint server for biology 2020 Pérez-Bermejo JA, Kang S, Rockwood SJ, Simoneau CR, Joy DA, Ramadoss GN, Silva AC, Flanigan WR, Li H, Nakamura K, Whitman JD, Ott M, Conklin BR, McDevitt TC -
Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells.
Cell reports 2020 Takahashi K, Jeong D, Wang S, Narita M, Jin X, Iwasaki M, Perli SD, Conklin BR, Yamanaka S -
Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair.
Nature communications 2020 Wienert B, Nguyen DN, Guenther A, Feng SJ, Locke MN, Wyman SK, Shin J, Kazane KR, Gregory GL, Carter MAM, Wright F, Conklin BR, Marson A, Richardson CD, Corn JE -
CRISPR off-target detection with DISCOVER-seq.
Nature protocols 2020 Wienert B, Wyman SK, Yeh CD, Conklin BR, Corn JE -
Cardiac Microtissues: Maladaptive Contractility of 3D Human Cardiac Microtissues to Mechanical Nonuniformity (Adv. Healthcare Mater. 8/2020).
Advanced Healthcare Materials 2020 Chenyan Wang, Sangmo Koo, Minok Park, Zacharias Vangelatos, Plansky Hoang, Bruce R. Conklin, Costas P. Grigoropoulos, Kevin E. Healy, Zhen Ma -
Maladaptive Contractility of 3D Human Cardiac Microtissues to Mechanical Nonuniformity.
Advanced healthcare materials 2020 Wang C, Koo S, Park M, Vangelatos Z, Hoang P, Conklin BR, Grigoropoulos CP, Healy KE, Ma Z -
Automated Design of Pluripotent Stem Cell Self-Organization.
Cell systems 2019 Libby ARG, Briers D, Haghighi I, Joy DA, Conklin BR, Belta C, McDevitt TC -
Abstract 934: Physical and Genetic Interaction Analyses in Human Pluripotent Stem Cell-Derived Cardiomyocytes to Study Protein Quality Control Disease in the Heart.
Circulation Research 2019 Juan A Perez-Bermejo, Luke M Judge, Kenneth Wu, Christina L Jensen, Robyn M Kaake, Danielle Swaney, Po-Lin So, Nevan J Krogan, Bruce R Conklin -
Phenotype-Based High-Throughput Classification of Long QT Syndrome Subtypes Using Human Induced Pluripotent Stem Cells.
Stem cell reports 2019 Yoshinaga D, Baba S, Makiyama T, Shibata H, Hirata T, Akagi K, Matsuda K, Kohjitani H, Wuriyanghai Y, Umeda K, Yamamoto Y, Conklin BR, Horie M, Takita J, Heike T -
Mapping cis-regulatory chromatin contacts in neural cells links neuropsychiatric disorder risk variants to target genes.
Nature genetics 2019 Song M, Yang X, Ren X, Maliskova L, Li B, Jones IR, Wang C, Jacob F, Wu K, Traglia M, Tam TW, Jamieson K, Lu SY, Ming GL, Li Y, Yao J, Weiss LA, Dixon JR, Judge LM, Conklin BR, Song H, Gan L, Shen Y -
A NOVEL ROLE FOR THE FTD-CAUSING PROTEIN PROGRANULIN IN APOPTOSIS SIGNALING.
Alzheimer's & Dementia 2019 Claire Clelland, J. Iker Etchegaray, Lay Kodama, Faten Sayed, Aradhana Sachdev, Yaqiao Li, David Le, Lihong Zhan, Yungui Zhou, Duc Duong, Nicholas T. Seyfried, Thomas G. Kukar, Li Xuan Tan, Aparna … -
On the road to a gene drive in mammals.
Nature 2019 Conklin BR -
MESP1 knock-down in human iPSC attenuates early vascular progenitor cell differentiation after completed primitive streak specification.
Developmental biology 2018 Eskildsen TV, Ayoubi S, Thomassen M, Burton M, Mandegar MA, Conklin BR, Jensen CH, Andersen DC, Sheikh SP -
Spatiotemporal mosaic self-patterning of pluripotent stem cells using CRISPR interference.
eLife 2018 Libby AR, Joy DA, So PL, Mandegar MA, Muncie JM, Mendoza-Camacho FN, Weaver VM, Conklin BR, McDevitt TC -
Contractile deficits in engineered cardiac microtissues as a result of MYBPC3 deficiency and mechanical overload.
Nature biomedical engineering 2018 Ma Z, Huebsch N, Koo S, Mandegar MA, Siemons B, Boggess S, Conklin BR, Grigoropoulos CP, Healy KE -
Quantitatively characterizing drug-induced arrhythmic contractile motions of human stem cell-derived cardiomyocytes.
Biotechnology and bioengineering 2018 Hoang P, Huebsch N, Bang SH, Siemons BA, Conklin BR, Healy KE, Ma Z, Jacquir S -
Quantitatively Characterizing Drug-Induced Arrhythmic Contractile Motions of Human Stem Cell-Derived Cardiomyocytes.
Biotechnology and Bioengineering 2018 Plansky Hoang, Nathaniel Huebsch, Shin Hyuk Bang, Brian A. Siemons, Bruce R. Conklin, Kevin E. Healy, Zhen Ma, Sabir Jacquir -
Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells.
Nature protocols 2018 Hoang P, Wang J, Conklin BR, Healy KE, Ma Z -
Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci Using Droplet Digital PCR.
Methods in molecular biology (Clifton, N.J.) 2018 Miyaoka Y, Mayerl SJ, Chan AH, Conklin BR -
TMOD-09. HUMAN PLURIPOTENT STEM CELL-BASED MODELS OF NEUROBLASTOMA.
Neuro-Oncology 2017 Miller Huang, Tina Zheng, Jeffrey Guo, Colin Sperring, Matthew Miller, Lauren McHenry, Qiqi Zhen, Branden Moriarity, Marianne Bronner, Bruce Conklin, David Largaespada, John Maris, Katherine Matthay, … -
Multi-Imaging Method to Assay the Contractile Mechanical Output of Micropatterned Human iPSC-Derived Cardiac Myocytes.
Circulation research 2017 Ribeiro AJS, Schwab O, Mandegar MA, Ang YS, Conklin BR, Srivastava D, Pruitt BL -
CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells.
Science (New York, N.Y.) 2016 Liu SJ, Horlbeck MA, Cho SW, Birk HS, Malatesta M, He D, Attenello FJ, Villalta JE, Cho MY, Chen Y, Mandegar MA, Olvera MP, Gilbert LA, Conklin BR, Chang HY, Weissman JS, Lim DA -
Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system.
Nature medicine 2016 Workman MJ, Mahe MM, Trisno S, Poling HM, Watson CL, Sundaram N, Chang CF, Schiesser J, Aubert P, Stanley EG, Elefanty AG, Miyaoka Y, Mandegar MA, Conklin BR, Neunlist M, Brugmann SA, Helmrath MA, … -
GENT-49. SYSTEMATIC IDENTIFICATION OF ESSENTIAL LONG NON-CODING RNA GENES IN GLIOBLASTOMA.
Neuro-Oncology 2016 Siyuan Liu, Max Horlbeck, Seung Woo Cho, Harjus Birk, Martina Malatesta, Daniel He, Jacqueline Villalta, Min Cho, Yuwen Chen, Mohammad Mandegar, Michael Olvera, Bruce Conklin, Howard Chang, Jonathan … -
BMP-SMAD-ID promotes reprogramming to pluripotency by inhibiting p16/INK4A-dependent senescence.
Proceedings of the National Academy of Sciences of the United States of America 2016 Hayashi Y, Hsiao EC, Sami S, Lancero M, Schlieve CR, Nguyen T, Yano K, Nagahashi A, Ikeya M, Matsumoto Y, Nishimura K, Fukuda A, Hisatake K, Tomoda K, Asaka I, Toguchida J, Conklin BR, Yamanaka S -
Organs-on-a-chip – Microphysiological platforms as in vitro models of cardiac and adipose tissue.
Toxicology Letters 2016 P. Loskill, A. Mathur, B.R. Conklin, A. Stahl, L.P. Lee, K.E. Healy -
Detecting Single-Nucleotide Substitutions Induced by Genome Editing.
Cold Spring Harbor protocols 2016 Miyaoka Y, Chan AH, Conklin BR -
Using Digital Polymerase Chain Reaction to Detect Single-Nucleotide Substitutions Induced by Genome Editing.
Cold Spring Harbor protocols 2016 Miyaoka Y, Chan AH, Conklin BR -
Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity.
Nature communications 2016 Nguyen DP, Miyaoka Y, Gilbert LA, Mayerl SJ, Lee BH, Weissman JS, Conklin BR, Wells JA -
Miniaturized iPS-Cell-Derived Cardiac Muscles for Physiologically Relevant Drug Response Analyses.
Scientific reports 2016 Huebsch N, Loskill P, Deveshwar N, Spencer CI, Judge LM, Mandegar MA, Fox CB, Mohamed TM, Ma Z, Mathur A, Sheehan AM, Truong A, Saxton M, Yoo J, Srivastava D, Desai TA, So PL, Healy KE, Conklin BR -
Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing.
Scientific reports 2016 Miyaoka Y, Berman JR, Cooper SB, Mayerl SJ, Chan AH, Zhang B, Karlin-Neumann GA, Conklin BR -
Generating trunk neural crest from human pluripotent stem cells.
Scientific reports 2016 Huang M, Miller ML, McHenry LK, Zheng T, Zhen Q, Ilkhanizadeh S, Conklin BR, Bronner ME, Weiss WA -
Efficient CRISPR/Cas9-Based Genome Engineering in Human Pluripotent Stem Cells.
Current protocols in human genetics 2016 Kime C, Mandegar MA, Srivastava D, Yamanaka S, Conklin BR, Rand TA -
Abstract 3230: Human stem cell-based model of MYCN-driven neuroblastoma.
Tumor Biology 2015 Miller Huang, Lauren K. McHenry, Matthew L. Miller, Grace E. Kim, Branden S. Moriarity, Yuichiro Miyaoka, Marianne E. Bronner, David A. Largaespada, Bruce R. Conklin, Hanlee P. Ji, John M. Maris, … -
Self-organizing human cardiac microchambers mediated by geometric confinement.
Nature communications 2015 Ma Z, Wang J, Loskill P, Huebsch N, Koo S, Svedlund FL, Marks NC, Hua EW, Grigoropoulos CP, Conklin BR, Healy KE -
Dysregulation of locus coeruleus development in congenital central hypoventilation syndrome.
Acta neuropathologica 2015 Nobuta H, Cilio MR, Danhaive O, Tsai HH, Tupal S, Chang SM, Murnen A, Kreitzer F, Bravo V, Czeisler C, Gokozan HN, Gygli P, Bush S, Weese-Mayer DE, Conklin B, Yee SP, Huang EJ, Gray PA, Rowitch D, … -
Loss of Gi G-Protein-Coupled Receptor Signaling in Osteoblasts Accelerates Bone Fracture Healing.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2015 Wang L, Hsiao EC, Lieu S, Scott M, O'Carroll D, Urrutia A, Conklin BR, Colnot C, Nissenson RA -
Structure-based discovery of NANOG variant with enhanced properties to promote self-renewal and reprogramming of pluripotent stem cells.
Proceedings of the National Academy of Sciences of the United States of America 2015 Hayashi Y, Caboni L, Das D, Yumoto F, Clayton T, Deller MC, Nguyen P, Farr CL, Chiu HJ, Miller MD, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Tomoda K, Conklin BR, Wilson IA, Yamanaka S, Fletterick … -
A non-invasive platform for functional characterization of stem-cell-derived cardiomyocytes with applications in cardiotoxicity testing.
Stem cell reports 2015 Maddah M, Heidmann JD, Mandegar MA, Walker CD, Bolouki S, Conklin BR, Loewke KE -
Human iPSC-based cardiac microphysiological system for drug screening applications.
Scientific reports 2015 Mathur A, Loskill P, Shao K, Huebsch N, Hong S, Marcus SG, Marks N, Mandegar M, Conklin BR, Lee LP, Healy KE -
Assessing the osteoblast transcriptome in a model of enhanced bone formation due to constitutive Gs-G protein signaling in osteoblasts.
Experimental cell research 2015 Wattanachanya L, Wang L, Millard SM, Lu WD, O'Carroll D, Hsiao EC, Conklin BR, Nissenson RA -
Gαi/o-coupled receptor signaling restricts pancreatic β-cell expansion.
Proceedings of the National Academy of Sciences of the United States of America 2015 Berger M, Scheel DW, Macias H, Miyatsuka T, Kim H, Hoang P, Ku GM, Honig G, Liou A, Tang Y, Regard JB, Sharifnia P, Yu L, Wang J, Coughlin SR, Conklin BR, Deneris ES, Tecott LH, German MS -
Astrocytic adenosine receptor A2A and Gs-coupled signaling regulate memory.
Nature neuroscience 2015 Orr AG, Hsiao EC, Wang MM, Ho K, Kim DH, Wang X, Guo W, Kang J, Yu GQ, Adame A, Devidze N, Dubal DB, Masliah E, Conklin BR, Mucke L -
Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cultured over Different Spatial Scales.
Tissue engineering. Part C, Methods 2015 Huebsch N, Loskill P, Mandegar MA, Marks NC, Sheehan AS, Ma Z, Mathur A, Nguyen TN, Yoo JC, Judge LM, Spencer CI, Chukka AC, Russell CR, So PL, Conklin BR, Healy KE -
Classifying the Electrophysiological Effects of Chronotropic Drugs on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes using Voltage Sensitive Dyes and Supervised Machine Learning.
Biophysical Journal 2015 Christopher M. Heylman, Rupsa Datta, Bruce R. Conklin, Steven C. George, Enrico Gratton -
Calcium transients closely reflect prolonged action potentials in iPSC models of inherited cardiac arrhythmia.
Stem cell reports 2014 Spencer CI, Baba S, Nakamura K, Hua EA, Sears MA, Fu CC, Zhang J, Balijepalli S, Tomoda K, Hayashi Y, Lizarraga P, Wojciak J, Scheinman MM, Aalto-Setälä K, Makielski JC, January CT, Healy KE, Kamp TJ,… -
Synthetic control of mammalian-cell motility by engineering chemotaxis to an orthogonal bioinert chemical signal.
Proceedings of the National Academy of Sciences of the United States of America 2014 Park JS, Rhau B, Hermann A, McNally KA, Zhou C, Gong D, Weiner OD, Conklin BR, Onuffer J, Lim WA -
Human induced pluripotent stem cell-based microphysiological tissue models of myocardium and liver for drug development.
Stem cell research & therapy 2013 Mathur A, Loskill P, Hong S, Lee J, Marcus SG, Dumont L, Conklin BR, Willenbring H, Lee LP, Healy KE -
Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation.
Orphanet journal of rare diseases 2013 Matsumoto Y, Hayashi Y, Schlieve CR, Ikeya M, Kim H, Nguyen TD, Sami S, Baba S, Barruet E, Nasu A, Asaka I, Otsuka T, Yamanaka S, Conklin BR, Toguchida J, Hsiao EC -
Three-dimensional filamentous human diseased cardiac tissue model.
Biomaterials 2013 Ma Z, Koo S, Finnegan MA, Loskill P, Huebsch N, Marks NC, Conklin BR, Grigoropoulos CP, Healy KE -
The C-terminus of the long AKAP13 isoform (AKAP-Lbc) is critical for development of compensatory cardiac hypertrophy.
Journal of molecular and cellular cardiology 2013 Taglieri DM, Johnson KR, Burmeister BT, Monasky MM, Spindler MJ, DeSantiago J, Banach K, Conklin BR, Carnegie GK -
A robust method to derive functional neural crest cells from human pluripotent stem cells.
American journal of stem cells 2013 Kreitzer FR, Salomonis N, Sheehan A, Huang M, Park JS, Spindler MJ, Lizarraga P, Weiss WA, So PL, Conklin BR -
AKAP13 Rho-GEF and PKD-binding domain deficient mice develop normally but have an abnormal response to β-adrenergic-induced cardiac hypertrophy.
PloS one 2013 Spindler MJ, Burmeister BT, Huang Y, Hsiao EC, Salomonis N, Scott MJ, Srivastava D, Carnegie GK, Conklin BR -
Structure of a novel winged-helix like domain from human NFRKB protein.
PloS one 2012 Kumar A, Möcklinghoff S, Yumoto F, Jaroszewski L, Farr CL, Grzechnik A, Nguyen P, Weichenberger CX, Chiu HJ, Klock HE, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Conklin BR, Fletterick RJ, Wilson IA -
Derivation conditions impact X-inactivation status in female human induced pluripotent stem cells.
Cell stem cell 2012 Tomoda K, Takahashi K, Leung K, Okada A, Narita M, Yamada NA, Eilertson KE, Tsang P, Baba S, White MP, Sami S, Srivastava D, Conklin BR, Panning B, Yamanaka S -
GO-Elite: a flexible solution for pathway and ontology over-representation.
Bioinformatics (Oxford, England) 2012 Zambon AC, Gaj S, Ho I, Hanspers K, Vranizan K, Evelo CT, Conklin BR, Pico AR, Salomonis N -
WikiPathways: building research communities on biological pathways.
Nucleic acids research 2011 Kelder T, van Iersel MP, Hanspers K, Kutmon M, Conklin BR, Evelo CT, Pico AR -
Model for long QT syndrome type 2 using human iPS cells demonstrates arrhythmogenic characteristics in cell culture.
Disease models & mechanisms 2011 Lahti AL, Kujala VJ, Chapman H, Koivisto AP, Pekkanen-Mattila M, Kerkelä E, Hyttinen J, Kontula K, Swan H, Conklin BR, Yamanaka S, Silvennoinen O, Aalto-Setälä K -
Mineral composition is altered by osteoblast expression of an engineered G(s)-coupled receptor.
Calcified tissue international 2011 Kazakia GJ, Speer D, Shanbhag S, Majumdar S, Conklin BR, Nissenson RA, Hsiao EC -
Blockade of receptor-activated G(i) signaling in osteoblasts in vivo leads to site-specific increases in cortical and cancellous bone formation.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2011 Millard SM, Louie AM, Wattanachanya L, Wronski TJ, Conklin BR, Nissenson RA -
Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice.
Stem cell research & therapy 2011 Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR -
Finding the right questions: exploratory pathway analysis to enhance biological discovery in large datasets.
PLoS biology 2010 Kelder T, Conklin BR, Evelo CT, Pico AR -
Stem cell pluripotency: alternative modes of transcription regulation.
Cell cycle (Georgetown, Tex.) 2010 Salomonis N, Conklin BR -
Reporter-based isolation of induced pluripotent stem cell- and embryonic stem cell-derived cardiac progenitors reveals limited gene expression variance.
Circulation research 2010 van Laake LW, Qian L, Cheng P, Huang Y, Hsiao EC, Conklin BR, Srivastava D -
AltAnalyze and DomainGraph: analyzing and visualizing exon expression data.
Nucleic acids research 2010 Emig D, Salomonis N, Baumbach J, Lengauer T, Conklin BR, Albrecht M -
Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation.
Proceedings of the National Academy of Sciences of the United States of America 2010 Salomonis N, Schlieve CR, Pereira L, Wahlquist C, Colas A, Zambon AC, Vranizan K, Spindler MJ, Pico AR, Cline MS, Clark TA, Williams A, Blume JE, Samal E, Mercola M, Merrill BJ, Conklin BR -
Gs G protein-coupled receptor signaling in osteoblasts elicits age-dependent effects on bone formation.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2010 Hsiao EC, Boudignon BM, Halloran BP, Nissenson RA, Conklin BR -
Ligand-mediated activation of an engineered gs g protein-coupled receptor in osteoblasts increases trabecular bone formation.
Molecular endocrinology (Baltimore, Md.) 2010 Hsiao EC, Millard SM, Louie A, Huang Y, Conklin BR, Nissenson RA -
Cloning mice and men: prohibiting the use of iPS cells for human reproductive cloning.
Cell stem cell 2010 Lo B, Parham L, Alvarez-Buylla A, Cedars M, Conklin B, Fisher S, Gates E, Giudice L, Halme DG, Hershon W, Kriegstein A, Kwok PY, Wagner R -
The BridgeDb framework: standardized access to gene, protein and metabolite identifier mapping services.
BMC bioinformatics 2010 van Iersel MP, Pico AR, Kelder T, Gao J, Ho I, Hanspers K, Conklin BR, Evelo CT -
G(i)-coupled GPCR signaling controls the formation and organization of human pluripotent colonies.
PloS one 2009 Nakamura K, Salomonis N, Tomoda K, Yamanaka S, Conklin BR -
Alternative splicing in the differentiation of human embryonic stem cells into cardiac precursors.
PLoS computational biology 2009 Salomonis N, Nelson B, Vranizan K, Pico AR, Hanspers K, Kuchinsky A, Ta L, Mercola M, Conklin BR -
Consent: criteria should be drawn up for tissue donors.
Nature 2009 Lo B, Conklin BR -
Journal club. A geneticist wonders why we need to sleep.
Nature 2009 Conklin BR -
Mining biological pathways using WikiPathways web services.
PloS one 2009 Kelder T, Pico AR, Hanspers K, van Iersel MP, Evelo C, Conklin BR -
AKAP10 (I646V) functional polymorphism predicts heart rate and heart rate variability in apparently healthy, middle-aged European-Americans.
Psychophysiology 2009 Neumann SA, Tingley WG, Conklin BR, Shrader CJ, Peet E, Muldoon MF, Jennings JR, Ferrell RE, Manuck SB -
Endogenous Gi signaling in osteoblasts participates in sexually dimorphic regulation of cancellous bone structure.
Bone 2009 S. Millard, A. Louie, C. Manalac, W. Lu, N. Cotte, T.J. Wronski, B. Conklin, R.A. Nissenson -
Lentiviral vectors and protocols for creation of stable hESC lines for fluorescent tracking and drug resistance selection of cardiomyocytes.
PloS one 2009 Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen… -
Obtaining consent for future research with induced pluripotent cells: opportunities and challenges.
PLoS biology 2009 Aalto-Setälä K, Conklin BR, Lo B -
SNPLogic: an interactive single nucleotide polymorphism selection, annotation, and prioritization system.
Nucleic acids research 2008 Pico AR, Smirnov IV, Chang JS, Yeh RF, Wiemels JL, Wiencke JK, Tihan T, Conklin BR, Wrensch M -
Presenting and exploring biological pathways with PathVisio.
BMC bioinformatics 2008 van Iersel MP, Kelder T, Pico AR, Hanspers K, Coort S, Conklin BR, Evelo C -
Engineering GPCR signaling pathways with RASSLs.
Nature methods 2008 Conklin BR, Hsiao EC, Claeysen S, Dumuis A, Srinivasan S, Forsayeth JR, Guettier JM, Chang WC, Pei Y, McCarthy KD, Nissenson RA, Wess J, Bockaert J, Roth BL -
WikiPathways: pathway editing for the people.
PLoS biology 2008 Pico AR, Kelder T, van Iersel MP, Hanspers K, Conklin BR, Evelo C -
Marking embryonic stem cells with a 2A self-cleaving peptide: a NKX2-5 emerald GFP BAC reporter.
PloS one 2008 Hsiao EC, Yoshinaga Y, Nguyen TD, Musone SL, Kim JE, Swinton P, Espineda I, Manalac C, deJong PJ, Conklin BR -
An Introduction to Pathway Analysis with GenMAPP.
NCI Nature Pathway Interaction Database 2008 Kristina Hanspers, Alexander R. Pico, Nathan Salomonis, Alexander C. Zambon, Karen Vranizan, Steven C. Lawlor, Kam D. Dahlquist, Scott W. Doniger, Bruce R. Conklin -
MicroRNA regulation of cell lineages in mouse and human embryonic stem cells.
Cell stem cell 2008 Ivey KN, Muth A, Arnold J, King FW, Yeh RF, Fish JE, Hsiao EC, Schwartz RJ, Conklin BR, Bernstein HS, Srivastava D -
Comparative analysis of wild-type and cAMP-deathless S49 lymphoma cells identifies genes and pathways of cAMP/PKA-promoted apoptosis.
The FASEB Journal 2008 Lingzhi Zhang, Alexander C Zambon, Karen Vranizan, Kanishka Pothula, Bruce R Conklin, Paul A Insel -
Osteoblast expression of an engineered Gs-coupled receptor dramatically increases bone mass.
Proceedings of the National Academy of Sciences of the United States of America 2008 Hsiao EC, Boudignon BM, Chang WC, Bencsik M, Peng J, Nguyen TD, Manalac C, Halloran BP, Conklin BR, Nissenson RA -
Modifying ligand-induced and constitutive signaling of the human 5-HT4 receptor.
PloS one 2007 Chang WC, Ng JK, Nguyen T, Pellissier L, Claeysen S, Hsiao EC, Conklin BR -
Conditional expression of a Gi-coupled receptor in osteoblasts results in trabecular osteopenia.
Endocrinology 2007 Peng J, Bencsik M, Louie A, Lu W, Millard S, Nguyen P, Burghardt A, Majumdar S, Wronski TJ, Halloran B, Conklin BR, Nissenson RA -
Gene expression signatures of cAMP/protein kinase A (PKA)-promoted, mitochondrial-dependent apoptosis. Comparative analysis of wild-type and cAMP-deathless S49 lymphoma cells.
The Journal of biological chemistry 2007 Zhang L, Zambon AC, Vranizan K, Pothula K, Conklin BR, Insel PA -
Engineering the melanocortin-4 receptor to control constitutive and ligand-mediated G(S) signaling in vivo.
PloS one 2007 Srinivasan S, Santiago P, Lubrano C, Vaisse C, Conklin BR -
Modeling insertional mutagenesis using gene length and expression in murine embryonic stem cells.
PloS one 2007 Nord AS, Vranizan K, Tingley W, Zambon AC, Hanspers K, Fong LG, Hu Y, Bacchetti P, Ferrin TE, Babbitt PC, Doniger SW, Skarnes WC, Young SG, Conklin BR -
GenMAPP 2: new features and resources for pathway analysis.
BMC bioinformatics 2007 Salomonis N, Hanspers K, Zambon AC, Vranizan K, Lawlor SC, Dahlquist KD, Doniger SW, Stuart J, Conklin BR, Pico AR -
Canonical Wnt signaling is a positive regulator of mammalian cardiac progenitors.
Proceedings of the National Academy of Sciences of the United States of America 2007 Kwon C, Arnold J, Hsiao EC, Taketo MM, Conklin BR, Srivastava D -
Interfacing silicon nanowires with mammalian cells.
Journal of the American Chemical Society 2007 Kim W, Ng JK, Kunitake ME, Conklin BR, Yang P -
Gene-trapped mouse embryonic stem cell-derived cardiac myocytes and human genetics implicate AKAP10 in heart rhythm regulation.
Proceedings of the National Academy of Sciences of the United States of America 2007 Tingley WG, Pawlikowska L, Zaroff JG, Kim T, Nguyen T, Young SG, Vranizan K, Kwok PY, Whooley MA, Conklin BR -
New tools to build synthetic hormonal pathways.
Proceedings of the National Academy of Sciences of the United States of America 2007 Conklin BR -
Development of hydrocephalus in mice expressing the G(i)-coupled GPCR Ro1 RASSL receptor in astrocytes.
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Sweger EJ, Casper KB, Scearce-Levie K, Conklin BR, McCarthy KD -
Integration of biological networks and gene expression data using Cytoscape.
Nature protocols 2007 Cline MS, Smoot M, Cerami E, Kuchinsky A, Landys N, Workman C, Christmas R, Avila-Campilo I, Creech M, Gross B, Hanspers K, Isserlin R, Kelley R, Killcoyne S, Lotia S, Maere S, Morris J, Ono K, … -
mGluR2 acts through inhibitory Galpha subunits to regulate transmission and long-term plasticity at hippocampal mossy fiber-CA3 synapses.
Proceedings of the National Academy of Sciences of the United States of America 2006 Nicholls RE, Zhang XL, Bailey CP, Conklin BR, Kandel ER, Stanton PK -
A Series of RASSLs Activated by a Single Agonist.
The FASEB Journal 2006 Peter Chang, Edward Hsiao, Alex C Zambon, Bruce R Conklin -
The International Gene Trap Consortium Website: a portal to all publicly available gene trap cell lines in mouse.
Nucleic acids research 2006 Nord AS, Chang PJ, Conklin BR, Cox AV, Harper CA, Hicks GG, Huang CC, Johns SJ, Kawamoto M, Liu S, Meng EC, Morris JH, Rossant J, Ruiz P, Skarnes WC, Soriano P, Stanford WL, Stryke D, von Melchner H, … -
Sustained preconditioning induced by cardiac transgenesis with the tetracycline transactivator.
American journal of physiology. Heart and circulatory physiology 2005 Turnbull L, Zhou HZ, Swigart PM, Turcato S, Karliner JS, Conklin BR, Simpson PC, Baker AJ -
Gene expression patterns define key transcriptional events in cell-cycle regulation by cAMP and protein kinase A.
Proceedings of the National Academy of Sciences of the United States of America 2005 Zambon AC, Zhang L, Minovitsky S, Kanter JR, Prabhakar S, Salomonis N, Vranizan K, Dubchak I, Conklin BR, Insel PA -
Cardiac transgenesis with the tetracycline transactivator changes myocardial function and gene expression.
Physiological genomics 2005 McCloskey DT, Turnbull L, Swigart PM, Zambon AC, Turcato S, Joho S, Grossman W, Conklin BR, Simpson PC, Baker AJ -
Engineered G protein coupled receptors reveal independent regulation of internalization, desensitization and acute signaling.
BMC biology 2005 Scearce-Levie K, Lieberman MD, Elliott HH, Conklin BR -
Identifying genetic networks underlying myometrial transition to labor.
Genome biology 2005 Salomonis N, Cotte N, Zambon AC, Pollard KS, Vranizan K, Doniger SW, Dolganov G, Conklin BR -
Constitutive activity of the melanocortin-4 receptor is maintained by its N-terminal domain and plays a role in energy homeostasis in humans.
The Journal of clinical investigation 2004 Srinivasan S, Lubrano-Berthelier C, Govaerts C, Picard F, Santiago P, Conklin BR, Vaisse C -
A public gene trap resource for mouse functional genomics.
Nature genetics 2004 Skarnes WC, von Melchner H, Wurst W, Hicks G, Nord AS, Cox T, Young SG, Ruiz P, Soriano P, Tessier-Lavigne M, Conklin BR, Stanford WL, Rossant J, International Gene Trap Consortium -
Time- and exercise-dependent gene regulation in human skeletal muscle.
Genome biology 2003 Zambon AC, McDearmon EL, Salomonis N, Vranizan KM, Johansen KL, Adey D, Takahashi JS, Schambelan M, Conklin BR -
Engineering the melanocortin-4 receptor to control G(s) signaling in vivo.
Annals of the New York Academy of Sciences 2003 Srinivasan S, Vaisse C, Conklin BR -
MAPPFinder: using Gene Ontology and GenMAPP to create a global gene-expression profile from microarray data.
Genome biology 2003 Doniger SW, Salomonis N, Dahlquist KD, Vranizan K, Lawlor SC, Conklin BR -
Regression approaches for microarray data analysis.
Journal of computational biology : a journal of computational molecular cell biology 2003 Segal MR, Dahlquist KD, Conklin BR -
GenMAPP, a new tool for viewing and analyzing microarray data on biological pathways.
Nature genetics 2002 Dahlquist KD, Salomonis N, Vranizan K, Lawlor SC, Conklin BR -
Tools for dissecting signaling pathways in vivo: receptors activated solely by synthetic ligands.
Methods in enzymology 2002 Scearce-Levie K, Coward P, Redfern CH, Conklin BR -
Engineering receptors activated solely by synthetic ligands (RASSLs).
Trends in pharmacological sciences 2001 Scearce-Levie K, Coward P, Redfern CH, Conklin BR -
Abnormal contraction caused by expression of G(i)-coupled receptor in transgenic model of dilated cardiomyopathy.
American journal of physiology. Heart and circulatory physiology 2001 Baker AJ, Redfern CH, Harwood MD, Simpson PC, Conklin BR -
Tetracycline-inducible system for photoreceptor-specific gene expression.
Investigative ophthalmology & visual science 2000 Chang MA, Horner JW, Conklin BR, DePinho RA, Bok D, Zack DJ -
Conditional expression of a Gi-coupled receptor causes ventricular conduction delay and a lethal cardiomyopathy.
Proceedings of the National Academy of Sciences of the United States of America 2000 Redfern CH, Degtyarev MY, Kwa AT, Salomonis N, Cotte N, Nanevicz T, Fidelman N, Desai K, Vranizan K, Lee EK, Coward P, Shah N, Warrington JA, Fishman GI, Bernstein D, Baker AJ, Conklin BR -
A critical period of vulnerability to hyperactive Gq signaling revealed by inducible and heart-specific expression of Gq* in transgenic mice.
Journal of Cardiac Failure 1999 Nathalie Cotte, Charles H. Redfern, Peter Mikus, Nathan Salomonis, Michael Y. Degtyarev, Peter S. Coward, Andrew Kwa, Tetsuo Shioi, Nila Shah, Tim McKinsey, Eric Olson, Seigo Izumo, Janet Warrington, … -
Genomic analysis of an inducible and reversible model of dilated cardiomyopathy.
Journal of Cardiac Failure 1999 Charles H. Redfern, Michael Y. Degtyarev, Kavin Desai, Andrew Kwa, Glenn I. Fishman, Daniel Bernstein, Anthony J. Baker, Bruce R. Conklin -
Chimeric G proteins allow a high-throughput signaling assay of Gi-coupled receptors.
Analytical biochemistry 1999 Coward P, Chan SD, Wada HG, Humphries GM, Conklin BR -
Conditional expression and signaling of a specifically designed Gi-coupled receptor in transgenic mice.
Nature biotechnology 1999 Redfern CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen L, Bujard H, Fishman GI, Conklin BR -
Controlling signaling with a specifically designed Gi-coupled receptor.
Proceedings of the National Academy of Sciences of the United States of America 1998 Coward P, Wada HG, Falk MS, Chan SD, Meng F, Akil H, Conklin BR -
The N-terminal extension of Galphaq is critical for constraining the selectivity of receptor coupling.
The Journal of biological chemistry 1997 Kostenis E, Degtyarev MY, Conklin BR, Wess J -
Molecular basis of receptor/G protein coupling selectivity studied by coexpression of wild type and mutant m2 muscarinic receptors with mutant G alpha(q) subunits.
Biochemistry 1997 Kostenis E, Conklin BR, Wess J -
Interactions of muscarinic receptors with the heterotrimeric G proteins Gq and G12: transduction of proliferative signals.
Journal of neurochemistry 1997 Burstein ES, Bräuner-Osborne H, Spalding TA, Conklin BR, Brann MR -
Carboxyl-terminal mutations of Gq alpha and Gs alpha that alter the fidelity of receptor activation.
Molecular pharmacology 1996 Conklin BR, Herzmark P, Ishida S, Voyno-Yasenetskaya TA, Sun Y, Farfel Z, Bourne HR -
Molecular mechanisms involved in muscarinic acetylcholine receptor-mediated G protein activation studied by insertion mutagenesis.
The Journal of biological chemistry 1996 Liu J, Blin N, Conklin BR, Wess J -
Identification of a receptor/G-protein contact site critical for signaling specificity and G-protein activation.
Proceedings of the National Academy of Sciences of the United States of America 1995 Liu J, Conklin BR, Blin N, Yun J, Wess J -
Coupling of the alpha 2A-adrenergic receptor to multiple G-proteins. A simple approach for estimating receptor-G-protein coupling efficiency in a transient expression system.
The Journal of biological chemistry 1994 Chabre O, Conklin BR, Brandon S, Bourne HR, Limbird LE -
G alpha 13 stimulates Na-H exchange.
The Journal of biological chemistry 1994 Voyno-Yasenetskaya T, Conklin BR, Gilbert RL, Hooley R, Bourne HR, Barber DL -
Homeostatic signals. Marriage of the flytrap and the serpent.
Nature 1994 Conklin BR, Bourne HR -
Mouse coat colour reconsidered.
Nature 1993 Conklin BR, Bourne HR -
Type II adenylylcyclase integrates coincident signals from Gs, Gi, and Gq.
The Journal of biological chemistry 1993 Lustig KD, Conklin BR, Herzmark P, Taussig R, Bourne HR -
Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors.
Cell 1993 Conklin BR, Bourne HR -
Substitution of three amino acids switches receptor specificity of Gq alpha to that of Gi alpha.
Nature 1993 Conklin BR, Farfel Z, Lustig KD, Julius D, Bourne HR -
A recombinant calcitonin receptor independently stimulates 3',5'-cyclic adenosine monophosphate and Ca2+/inositol phosphate signaling pathways.
Molecular endocrinology (Baltimore, Md.) 1992 Chabre O, Conklin BR, Lin HY, Lodish HF, Wilson E, Ives HE, Catanzariti L, Hemmings BA, Bourne HR -
Hormonal stimulation of adenylyl cyclase through Gi-protein beta gamma subunits.
Nature 1992 Federman AD, Conklin BR, Schrader KA, Reed RR, Bourne HR -
Gz-mediated hormonal inhibition of cyclic AMP accumulation.
Science (New York, N.Y.) 1992 Wong YH, Conklin BR, Bourne HR -
Recombinant Gq alpha. Mutational activation and coupling to receptors and phospholipase C.
The Journal of biological chemistry 1992 Conklin BR, Chabre O, Wong YH, Federman AD, Bourne HR -
Detection of coincident signals by G proteins and adenylyl cyclase.
Cold Spring Harbor symposia on quantitative biology 1992 Bourne HR, Lustig KD, Wong YH, Conklin BR -
Carbachol-induced reverse transformation of Chinese hamster ovary cells transfected with and expressing the m5 muscarinic acetylcholine receptor.
FEBS letters 1989 Felder CC, Ma AL, Conklin BR -
Stimulation of arachidonic acid release and inhibition of mitogenesis by cloned genes for muscarinic receptor subtypes stably expressed in A9 L cells.
Proceedings of the National Academy of Sciences of the United States of America 1988 Conklin BR, Brann MR, Buckley NJ, Ma AL, Bonner TI, Axelrod J -
Distinct bradykinin receptors mediate stimulation of prostaglandin synthesis by endothelial cells and fibroblasts.
The Journal of pharmacology and experimental therapeutics 1988 Conklin BR, Burch RM, Steranka LR, Axelrod J