Brian Shy, MD, PhD
Assistant Professor
Laboratory Medicine
School of Medicine

Publications (24)

Top publication keywords:
Gene EditingDNA, Single-StrandedGenetic EngineeringMouse Embryonic Stem CellsConsumer Product SafetyCRISPR-Cas SystemsWnt Signaling PathwayMutagenesis, InsertionalRecombinational DNA RepairGene Transfer TechniquesTranscription Factor 7-Like 1 ProteinGenomeCell EngineeringDNA End-Joining RepairCell- and Tissue-Based Therapy

• Engineering an Escherichia coli strain for production of long single-stranded DNA.
  Nucleic acids research 2024 Shen K, Flood JJ, Zhang Z, Ha A, Shy BR, Dueber JE, Douglas SM
• Engineering an Escherichia coli strain for production of long single-stranded DNA.
  bioRxiv : the preprint server for biology 2024 Shen K, Flood JJ, Zhang Z, Ha A, Shy BR, Dueber JE, Douglas SM
• Ultra-high efficiency T cell reprogramming at multiple loci with SEED-Selection.
  bioRxiv : the preprint server for biology 2024 Chang CR, Vykunta VS, Goodman DB, Muldoon JJ, Nyberg WA, Liu C, Allain V, Rothrock A, Wang CH, Marson A, Shy BR, Eyquem J
• Base-editing mutagenesis maps alleles to tune human T cell functions.
  Nature 2023 Schmidt R, Ward CC, Dajani R, Armour-Garb Z, Ota M, Allain V, Hernandez R, Layeghi M, Xing G, Goudy L, Dorovskyi D, Wang C, Chen YY, Ye CJ, Shy BR, Gilbert LA, Eyquem J, Pritchard JK, Dodgson SE, …
• Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes.
  Nature biomedical engineering 2023 Foss DV, Muldoon JJ, Nguyen DN, Carr D, Sahu SU, Hunsinger JM, Wyman SK, Krishnappa N, Mendonsa R, Schanzer EV, Shy BR, Vykunta VS, Allain V, Li Z, Marson A, Eyquem J, Wilson RC

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• Dynamic phosphatase-recruitment controls B-cell selection and oncogenic signaling.
  bioRxiv : the preprint server for biology 2023 Lee J, Robinson ME, Sun R, Kume K, Ma N, Cosgun KN, Chan LN, Leveille E, Geng H, Vykunta VS, Shy BR, Marson A, Katz S, Chen J, Paietta E, Meffre E, Vaidehi N, Müschen M
• Pooled screening of CAR T cells identifies diverse immune signaling domains for next-generation immunotherapies.
  Science translational medicine 2022 Goodman DB, Azimi CS, Kearns K, Talbot A, Garakani K, Garcia J, Patel N, Hwang B, Lee D, Park E, Vykunta VS, Shy BR, Ye CJ, Eyquem J, Marson A, Bluestone JA, Roybal KT
• High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails.
  Nature biotechnology 2022 Shy BR, Vykunta VS, Ha A, Talbot A, Roth TL, Nguyen DN, Pfeifer WG, Chen YY, Blaeschke F, Shifrut E, Vedova S, Mamedov MR, Chung JJ, Li H, Yu R, Wu D, Wolf J, Martin TG, Castro CE, Ye L, Esensten JH, …
• Proceedings from the Blood and Marrow Transplant Clinical Trials Network Myeloma Intergroup Workshop on Immune and Cellular Therapy in Multiple Myeloma.
  Transplantation and cellular therapy 2022 Holstein SA, Asimakopoulos F, Azab AK, Bianchi G, Bhutani M, Crews LA, Cupedo T, Giles H, Gooding S, Hillengass J, John L, Kaiser S, Lee L, Maclachlan K, Pasquini MC, Pichiorri F, Shah N, Shokeen M, …
• CRISPR-Cas9-mediated nuclear transport and genomic integration of nanostructured genes in human primary cells.
  Nucleic acids research 2022 Lin-Shiao E, Pfeifer WG, Shy BR, Saffari Doost M, Chen E, Vykunta VS, Hamilton JR, Stahl EC, Lopez DM, Sandoval Espinoza CR, Deyanov AE, Lew RJ, Poirer MG, Marson A, Castro CE, Doudna JA
• Polyclonal Regulatory T Cell Manufacturing Under cGMP: A Decade of Experience.
  Frontiers in immunology 2021 Balcerek J, Shy BR, Putnam AL, Masiello LM, Lares A, Dekovic F, Acevedo L, Lee MR, Nguyen V, Liu W, Paruthiyil S, Xu J, Leinbach AS, Bluestone JA, Tang Q, Esensten JH
• Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineering.
  Cell reports 2021 Hamilton JR, Tsuchida CA, Nguyen DN, Shy BR, McGarrigle ER, Sandoval Espinoza CR, Carr D, Blaeschke F, Marson A, Doudna JA
• Hospital-Based Donor Recruitment and Predonation Serologic Testing for COVID-19 Convalescent Plasma.
  American journal of clinical pathology 2021 Balcerek J, Trejo E, Levine K, Couey P, Kornberg ZV, Rogine C, Young C, Li PJ, Shy BR, Taylor JE, Bakhtary S, Friedlander T, Lynch KL, Bern C, Esensten JH
• Magnitude and Kinetics of Anti-Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Responses and Their Relationship to Disease Severity.
  Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2021 Lynch KL, Whitman JD, Lacanienta NP, Beckerdite EW, Kastner SA, Shy BR, Goldgof GM, Levine AG, Bapat SP, Stramer SL, Esensten JH, Hightower AW, Bern C, Wu AHB
• SARS-CoV-2 seroprevalence and neutralizing activity in donor and patient blood.
  Nature communications 2020 Ng DL, Goldgof GM, Shy BR, Levine AG, Balcerek J, Bapat SP, Prostko J, Rodgers M, Coller K, Pearce S, Franz S, Du L, Stone M, Pillai SK, Sotomayor-Gonzalez A, Servellita V, Martin CSS, Granados A, …
• Evaluation of SARS-CoV-2 serology assays reveals a range of test performance.
  Nature biotechnology 2020 Whitman JD, Hiatt J, Mowery CT, Shy BR, Yu R, Yamamoto TN, Rathore U, Goldgof GM, Whitty C, Woo JM, Gallman AE, Miller TE, Levine AG, Nguyen DN, Bapat SP, Balcerek J, Bylsma SA, Lyons AM, Li S, Wong …
• Magnitude and kinetics of anti-SARS-CoV-2 antibody responses and their relationship to disease severity.
  Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2020 Lynch KL, Whitman JD, Lacanienta NP, Beckerdite EW, Kastner SA, Shy BR, Goldgof GM, Levine AG, Bapat SP, Stramer SL, Esensten JH, Hightower AW, Bern C, Wu AHB
• SARS-CoV-2 seroprevalence and neutralizing activity in donor and patient blood from the San Francisco Bay Area.
  medRxiv : the preprint server for health sciences 2020 Ng DL, Goldgof GM, Shy BR, Levine AG, Balcerek J, Bapat SP, Prostko J, Rodgers M, Coller K, Pearce S, Franz S, Du L, Stone M, Pillai SK, Sotomayor-Gonzalez A, Servellita V, Martin CSS, Granados A, …
• Test performance evaluation of SARS-CoV-2 serological assays.
  medRxiv : the preprint server for health sciences 2020 Whitman JD, Hiatt J, Mowery CT, Shy BR, Yu R, Yamamoto TN, Rathore U, Goldgof GM, Whitty C, Woo JM, Gallman AE, Miller TE, Levine AG, Nguyen DN, Bapat SP, Balcerek J, Bylsma SA, Lyons AM, Li S, Wong …
• Co-incident insertion enables high efficiency genome engineering in mouse embryonic stem cells.
  Nucleic acids research 2016 Shy BR, MacDougall MS, Clarke R, Merrill BJ
• Regulation of Tcf7l1 DNA binding and protein stability as principal mechanisms of Wnt/β-catenin signaling.
  Cell reports 2013 Shy BR, Wu CI, Khramtsova GF, Zhang JY, Olopade OI, Goss KH, Merrill BJ
• MpzR98C arrests Schwann cell development in a mouse model of early-onset Charcot-Marie-Tooth disease type 1B.
  Brain : a journal of neurology 2012 Saporta MA, Shy BR, Patzko A, Bai Y, Pennuto M, Ferri C, Tinelli E, Saveri P, Kirschner D, Crowther M, Southwood C, Wu X, Gow A, Feltri ML, Wrabetz L, Shy ME
• Function of Wnt/β-catenin in counteracting Tcf3 repression through the Tcf3-β-catenin interaction.
  Development (Cambridge, England) 2012 Wu CI, Hoffman JA, Shy BR, Ford EM, Fuchs E, Nguyen H, Merrill BJ
• Opposing effects of Tcf3 and Tcf1 control Wnt stimulation of embryonic stem cell self-renewal.
  Nature cell biology 2011 Yi F, Pereira L, Hoffman JA, Shy BR, Yuen CM, Liu DR, Merrill BJ