Bassem Al-Sady, PhD
Associate Professor
Microbiology and Immunology
School of Medicine

Awards

Show all (1) Hide

• New Innovator Award (DP2), National Institutes of Health, 2016-2021

Education & Training

Show all (3) Hide

• Diversity, Equity, and Inclusion Champion Training University of California 09/2020
• Ph.D. Plant and Microbial Biology University of California Berkeley 2006
• Master of Biochemistry Biochemistry Oxford University 2000

Websites

Show all (2) Hide

• Al-Sady lab (alsadylab.ucsf.edu)
• Posts on X ASadylab (X.com)

Grants and Projects

Show all (3) Hide

• Tracking how molecular machines propagate epigenetic information in time and space, NIH, 2021-2026
• Probing heterochromatin growth and memory dynamics in live single cells, National Science Foundation (NSF), 2021-2025
• Reconstructing dynamic epigenetic genome partitioning in single stem cells, NIH, 2016-2021

Publications (29)

Top publication keywords:
Chromosomal Proteins, Non-HistoneHeterochromatinGenomic InstabilityHistocompatibility AntigensRNA, UntranslatedHistone-Lysine N-MethyltransferaseNucleosomesProtein MultimerizationSchizosaccharomyces pombe ProteinsHistonesPhytochromeCell Cycle ProteinsGene SilencingChromatin Assembly and DisassemblySchizosaccharomyces

• A systematic quantitative approach comprehensively defines domain-specific functional pathways linked to Schizosaccharomyces pombe heterochromatin regulation.
  Nucleic acids research 2024 Muhammad A, Sarkadi Z, Mazumder A, Ait Saada A, van Emden T, Capella M, Fekete G, Suma Sreechakram VN, Al-Sady B, Lambert SAE, Papp B, Barrales RR, Braun S
• Phosphorylation of HP1/Swi6 relieves competition with Suv39/Clr4 on nucleosomes and enables H3K9 trimethyl spreading.
  bioRxiv : the preprint server for biology 2024 Kennedy DR, Lemiere J, Tan C, Simental E, Braxton J, Maxwell RA, Amine AA, Al-Sady B
• The nuclear periphery confers repression on H3K9me2-marked genes and transposons to shape cell fate.
  bioRxiv : the preprint server for biology 2024 Marin H, Simental E, Allen C, Martin E, Panning B, Al-Sady B, Buchwalter A
• A systematic quantitative approach comprehensively defines domain-specific functional pathways linked to Schizosaccharomyces pombe heterochromatin regulation.
  bioRxiv : the preprint server for biology 2024 Muhammad A, Sarkadi Z, van Emden T, Mazumder A, Capella M, Fekete G, Sreechakram VNS, Al-Sady B, Papp B, Barrales RR, Braun S
• Regulation of the heterochromatin spreading reaction by trans-acting factors.
  Open biology 2023 Hamali B, Amine AAA, Al-Sady B

Show all (24 more) Hide

• A dual, catalytic role for the fission yeast Ccr4-Not complex in gene silencing and heterochromatin spreading.
  Genetics 2023 Challal D, Menant A, Goksal C, Leroy E, Al-Sady B, Rougemaille M
• Proteome effects of genome-wide single gene perturbations.
  Nature communications 2022 Öztürk M, Freiwald A, Cartano J, Schmitt R, Dejung M, Luck K, Al-Sady B, Braun S, Levin M, Butter F
• Local chromatin context regulates the genetic requirements of the heterochromatin spreading reaction.
  PLoS genetics 2022 Greenstein RA, Ng H, Barrales RR, Tan C, Braun S, Al-Sady B
• The histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states.
  Cell reports 2021 Murawska M, Greenstein RA, Schauer T, Olsen KCF, Ng H, Ladurner AG, Al-Sady B, Braun S
• Heterodimerization of H3K9 histone methyltransferases G9a and GLP activates methyl reading and writing capabilities.
  The Journal of biological chemistry 2021 Sanchez NA, Kallweit LM, Trnka MJ, Clemmer CL, Al-Sady B
• Set1/COMPASS repels heterochromatin invasion at euchromatic sites by disrupting Suv39/Clr4 activity and nucleosome stability.
  Genes & development 2019 Greenstein RA, Barrales RR, Sanchez NA, Bisanz JE, Braun S, Al-Sady B
• Epigenetic fates of gene silencing established by heterochromatin spreading in cell identity and genome stability.
  Current genetics 2018 Greenstein RA, Al-Sady B
• Noncoding RNA-nucleated heterochromatin spreading is intrinsically labile and requires accessory elements for epigenetic stability.
  eLife 2018 Greenstein RA, Jones SK, Spivey EC, Rybarski JR, Finkelstein IJ, Al-Sady B
• Chromatin Curtains: A Single-Molecule Method for Visualizing Histone Marks on Chromatin Substrates In Vitro.
  Biophysical Journal 2018 Liv E. Jensen, Bassem Al-Sady, Sy Redding
• Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics.
  PloS one 2016 Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
• Molecular convergence of clock and photosensory pathways through PIF3-TOC1 interaction and co-occupancy of target promoters.
  Proceedings of the National Academy of Sciences of the United States of America 2016 Soy J, Leivar P, González-Schain N, Martín G, Diaz C, Sentandreu M, Al-Sady B, Quail PH, Monte E
• Intrinsic Toxicity of Unchecked Heterochromatin Spread Is Suppressed by Redundant Chromatin Boundary Functions in Schizosacchromyces pombe.
  G3 (Bethesda, Md.) 2015 Garcia JF, Al-Sady B, Madhani HD
• Division of labor between the chromodomains of HP1 and Suv39 methylase enables coordination of heterochromatin spread.
  Molecular cell 2013 Al-Sady B, Madhani HD, Narlikar GJ
• Reconstitution of nucleosome demethylation and catalytic properties of a Jumonji histone demethylase.
  Chemistry & biology 2013 Shiau C, Trnka MJ, Bozicevic A, Ortiz Torres I, Al-Sady B, Burlingame AL, Narlikar GJ, Fujimori DG
• Dynamic antagonism between phytochromes and PIF family basic helix-loop-helix factors induces selective reciprocal responses to light and shade in a rapidly responsive transcriptional network in Arabidopsis.
  The Plant cell 2012 Leivar P, Tepperman JM, Cohn MM, Monte E, Al-Sady B, Erickson E, Quail PH
• Chromodomain-mediated oligomerization of HP1 suggests a nucleosome-bridging mechanism for heterochromatin assembly.
  Molecular cell 2011 Canzio D, Chang EY, Shankar S, Kuchenbecker KM, Simon MD, Madhani HD, Narlikar GJ, Al-Sady B
• The Arabidopsis phytochrome-interacting factor PIF7, together with PIF3 and PIF4, regulates responses to prolonged red light by modulating phyB levels.
  The Plant cell 2008 Leivar P, Monte E, Al-Sady B, Carle C, Storer A, Alonso JM, Ecker JR, Quail PH
• Mechanistic duality of transcription factor function in phytochrome signaling.
  Proceedings of the National Academy of Sciences of the United States of America 2008 Al-Sady B, Kikis EA, Monte E, Quail PH
• Out of the dark: how the PIFs are unmasking a dual temporal mechanism of phytochrome signalling.
  Journal of experimental botany 2007 Monte E, Al-Sady B, Leivar P, Quail PH
• Photoactivated phytochrome induces rapid PIF3 phosphorylation prior to proteasome-mediated degradation.
  Molecular cell 2006 Al-Sady B, Ni W, Kircher S, Schäfer E, Quail PH
• The phytochrome-interacting transcription factor, PIF3, acts early, selectively, and positively in light-induced chloroplast development.
  Proceedings of the National Academy of Sciences of the United States of America 2004 Monte E, Tepperman JM, Al-Sady B, Kaczorowski KA, Alonso JM, Ecker JR, Li X, Zhang Y, Quail PH
• A novel molecular recognition motif necessary for targeting photoactivated phytochrome signaling to specific basic helix-loop-helix transcription factors.
  The Plant cell 2004 Khanna R, Huq E, Kikis EA, Al-Sady B, Lanzatella C, Quail PH
• Phytochrome-interacting factor 1 is a critical bHLH regulator of chlorophyll biosynthesis.
  Science (New York, N.Y.) 2004 Huq E, Al-Sady B, Hudson M, Kim C, Apel K, Quail PH
• Nuclear translocation of the photoreceptor phytochrome B is necessary for its biological function in seedling photomorphogenesis.
  The Plant journal : for cell and molecular biology 2003 Huq E, Al-Sady B, Quail PH