Abigail (Abby) Buchwalter
Associate Professor
Cardiovascular Research Inst
School of Medicine

Interests

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• nuclear organization
• cell biology
• cell identity

Grants and Projects

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• Understanding mechanisms of heterochromatin tethering at the nuclear lamina, NIH, 2021-2026
• Identifying links between the nuclear pore complex and the cell cycle, NIH, 2012-2015
• Mechanism and regulation of LULL 1-directed NE enrichment of TorsinA, NIH, 2009-2011

Publications (27)

Top publication keywords:
HeterochromatinDNA, RibosomalMembrane ProteinsProgeriaCell NucleolusCell NucleusMouse Embryonic Stem CellsNuclear PoreNuclear ProteinsNuclear EnvelopeLamin Type BLamin Type AIntermediate FilamentsCell ShapeNuclear Lamina

• Coaching from the sidelines: the nuclear periphery in genome regulation.
  Nature reviews. Genetics 2019 Buchwalter A, Kaneshiro JM, Hetzer MW
• Nucleolar expansion and elevated protein translation in premature aging.
  Nature communications 2017 Buchwalter A, Hetzer MW
• Nup50 is required for cell differentiation and exhibits transcription-dependent dynamics.
  Molecular biology of the cell 2014 Buchwalter AL, Liang Y, Hetzer MW
• LULL1 retargets TorsinA to the nuclear envelope revealing an activity that is impaired by the DYT1 dystonia mutation.
  Molecular biology of the cell 2009 Vander Heyden AB, Naismith TV, Snapp EL, Hodzic D, Hanson PI
• C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin.
  eLife 2025 Mella J, Volk RF, Zaro BW, Buchwalter A

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• The nuclear periphery confers repression on H3K9me2-marked genes and transposons to shape cell fate.
  Nature cell biology 2025 Marin HC, Allen C, Simental E, Martin EW, Panning B, Al-Sady B, Buchwalter A
• Lamin B1 and LAP2β resist cytoskeletal force to maintain lamin A/C meshwork organization and preserve nuclear integrity.
  Molecular biology of the cell 2025 Alabi Y, Aksenova V, Arnaoutov A, Marin H, Dasso M, Buchwalter A
• C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin.
  bioRxiv : the preprint server for biology 2025 Mella J, Volk R, Zaro B, Buchwalter A
• Proteome Birthdating: A Single-Sample Approach for Measuring Global Turnover Dynamics and "Protein Age".
  Bio-protocol 2025 Meadow ME, Broas S, Hoare M, Ahmed M, Alimohammadi F, Welle KA, Swovick K, Hryhorenko JR, Jain A, Martinez JC, Seluanov A, Gorbunova V, Buchwalter A, Ghaemmaghami S
• Modeling the consequences of age-linked rDNA hypermethylation with dCas9-directed DNA methylation in human cells.
  PloS one 2024 Blokhina Y, Buchwalter A
• What does it take to build a nucleus?
  Nature reviews. Molecular cell biology 2024 Buchwalter A
• The nuclear periphery confers repression on H3K9me2-marked genes and transposons to shape cell fate.
  bioRxiv : the preprint server for biology 2024 Marin H, Simental E, Allen C, Martin E, Panning B, Al-Sady B, Buchwalter A
• Proteome Birthdating Reveals Age-Selectivity of Protein Ubiquitination.
  Molecular & cellular proteomics : MCP 2024 Meadow ME, Broas S, Hoare M, Alimohammadi F, Welle KA, Swovick K, Hryhorenko JR, Martinez JC, Biashad SA, Seluanov A, Gorbunova V, Buchwalter A, Ghaemmaghami S
• In vivo protein turnover rates in varying oxygen tensions nominate MYBBP1A as a mediator of the hyperoxia response.
  Science advances 2023 Chen X, Haribowo AG, Baik AH, Fossati A, Stevenson E, Chen YR, Reyes NS, Peng T, Matthay MA, Traglia M, Pico AR, Jarosz DF, Buchwalter A, Ghaemmaghami S, Swaney DL, Jain IH
• Long lifetime and tissue-specific accumulation of lamin A/C in Hutchinson-Gilford progeria syndrome.
  The Journal of cell biology 2023 Hasper J, Welle K, Swovick K, Hryhorenko J, Ghaemmaghami S, Buchwalter A
• Modeling the consequences of age-linked rDNA hypermethylation with dCas9-directed DNA methylation in human cells.
  bioRxiv : the preprint server for biology 2023 Blokhina Y, Buchwalter A
• Intermediate, but not average: The unusual lives of the nuclear lamin proteins.
  Current opinion in cell biology 2023 Buchwalter A
• Long lifetime and selective accumulation of the A-type lamins accounts for the tissue specificity of Hutchinson-Gilford progeria syndrome.
  bioRxiv : the preprint server for biology 2023 Hasper J, Welle K, Swovick K, Hryhorenko J, Ghaemmaghami S, Buchwalter A
• Turnover and replication analysis by isotope labeling (TRAIL) reveals the influence of tissue context on protein and organelle lifetimes.
  Molecular systems biology 2023 Hasper J, Welle K, Hryhorenko J, Ghaemmaghami S, Buchwalter A
• Moving fast and breaking things: Incidence and repair of DNA damage within ribosomal DNA repeats.
  Mutation research 2020 Blokhina YP, Buchwalter A
• Selective clearance of the inner nuclear membrane protein emerin by vesicular transport during ER stress.
  eLife 2019 Buchwalter A, Schulte R, Tsai H, Capitanio J, Hetzer M
• Access of torsinA to the inner nuclear membrane is activity dependent and regulated in the endoplasmic reticulum.
  Journal of cell science 2015 Goodchild RE, Buchwalter AL, Naismith TV, Holbrook K, Billion K, Dauer WT, Liang CC, Dear ML, Hanson PI
• Nuclear pores set the speed limit for mitosis.
  Cell 2014 Buchwalter A, Hetzer MW
• Static retention of the lumenal monotopic membrane protein torsinA in the endoplasmic reticulum.
  The EMBO journal 2011 Vander Heyden AB, Naismith TV, Snapp EL, Hanson PI
• Expression of VACM-1/cul5 mutant in endothelial cells induces MAPK phosphorylation and maspin degradation and converts cells to the angiogenic phenotype.
  Microvascular research 2007 Buchwalter A, Van Dort C, Schultz S, Smith R, Le IP, Abbott JL, Oosterhouse E, Johnson AE, Hansen-Smith F, Burnatowska-Hledin M
• Estrogen-dependent growth and estrogen receptor (ER)-alpha concentration in T47D breast cancer cells are inhibited by VACM-1, a cul 5 gene.
  Molecular and cellular biochemistry 2006 Johnson AE, Le IP, Buchwalter A, Burnatowska-Hledin MA
• R7BP augments the function of RGS7*Gbeta5 complexes by a plasma membrane-targeting mechanism.
  The Journal of biological chemistry 2006 Drenan RM, Doupnik CA, Jayaraman M, Buchwalter AL, Kaltenbronn KM, Huettner JE, Linder ME, Blumer KJ